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Cherbal A.,Jijel University | Kebieche M.,Jijel University | Madani K.,University of Abderrahmane Mira de Bejaia | El-Adawi H.,Genetic Engineering and Biotechnology Institute
Asian Journal of Plant Sciences | Year: 2012

Pistacia lentiscus is known since antiquity for its medicinal properties. Its leaves have anti¬inflammatory, antibacterial, antifungal, antipyretic, hepatoprotective and anti-diarrheal effects. They are also used in the treatment of several other diseases. This present study aims to the valorization of the hydro- methanolic extract of Pistacia lentiscus leaves as an antioxidant. First, a hydro-methanolic extraction of the total phenolic compounds contained in the leaves of Pistacia lentiscus was measured and then quantitative analysis of the polyphenol, flavonoids and tannins contents were carried out. The antioxidant activity was evaluated by measuring the reduction of hydrogen peroxide and the scavenging ability with respect to a relatively stable free radical (DPPH). Our results revealed that, the leaves extract has a significant scavenging activity, where it reached up to 76.4% at 200 fig mL-1 which is comparable with that of a-tocopherol. A moderate reducing power (37.04%) of H202 was recorded at 50 fig mL-1. In addition, the Pistacia lentiscus leaves extract was rich in polyphenols which seem to have a comparable antioxidant capacity and even more significant than that of the standards. © 2012 Asian Network for Scientific Information. Source


Sholkamy E.N.,King Saud University | Abdel-Megeed A.,King Saud University | Abdel-Megeed A.,Alexandria University | Elnakieb,Genetic Engineering and Biotechnology Institute | And 2 more authors.
Journal of Pure and Applied Microbiology | Year: 2012

Microalgae are a potential source of biodiesel. Isolates of the present study were isolated from Wadi-henifa, Rhiyad, Saudi Arabia. The urgent need for an alternative and sustainable energy has created renewed interest to analyse the microalgae for biodiesel production. The greatest lipid content reached 20.2, 16.4, 9.7 and 12.3% under the optimal conditions of nitrate concentration (0.75 g/l), temperature (24 and 30°C), salinity (0.05 and 0.001 mole/l) and pH (8 with Chlorellavulgarus and 9 with other strains), Chlorella vulgarusArthrospiraplatensisGomont and Spirulina major, respectively. It was demonstrated that the obtained model was effective for predicting lipid productivity of the isolated microalgae. The maximum protein content was at 24°C for Chlorella vulgarus,ArthrospiraplatensisGomont and Spirulinamajorkütz, (53, 56.8 and 54% respectively), while the maximum protein content ofArthrospira maxima was at 30°C (56.2%).The optimum protein content was found at pH9forArthrospiraplatensisGomont, Spirulina major kützandArthrospira maxima(48.47, 55.47 and 63.25% respectively) while in case of Chlorella vulgaruswas at pH 8 (51%).The maximum protein content was 76.96% at 0.001 moleNacl/L, 54, 75.38 and 75.09% at 0.05 mole/L for Chlorella vulgarus,Spirulina major kütz, Arthrospira maxima andArthrospiraplatensisGomont respectively. Results of this study revealed thatthe mention optimization conditions enhanced protein content of the tested isolates. ArthrospiraplatensisGomont, SpirulinamajorkützandArthrospira maxima are promising organisms with high nutritional value for animal and human beings. Source


EL-Kenawy A.E.L.-M.,Genetic Engineering and Biotechnology Institute
International Journal of Cancer Research and Prevention | Year: 2013

HCC is unique among cancers occurring mostly in patients with chronic inflammation and cirrhosis. It continues to be the sixth most common neoplasm worldwide and due to its high mortality the third most common cause of cancer mortality, causing 9.2% of all cancer deaths. HCC has become the second most prevalent cancer among men in Egypt. Of all the HCC cases, it is estimated that 66% are attributable to hepatitis B virus (HBV) and 42% are attributable to Hepatitis C virus (HCV), assuming that the relative risk of disease in both carriers is 20. The incidence of HCC is expected to increase in the next two decades, largely due to hepatitis C infection and secondary cirrhosis. The prognosis is poor unless HCC is diagnosed and treated early. In Egypt, there is a growing incidence of HCC (10-120/100,000), which represent the leading cause of death from all cancer sites. HCC has nearly doubled over the last decade from 4.0% in 1993 to 7.2% in 2002 among patients with chronic liver disease (CLD). In this review we present a summary of all the recent information on HCC and mention all the factors that might contribute to hepatocarcinogenesis, beside our recommendation approach at the end of the article which would help our policy makers in developing a new strategy to reduce the increasing incidence of HCC in Egypt. © Nova Science Publishers, Inc. Source


Ellis J.S.,University of Missouri | Guloglu F.B.,University of Missouri | Guloglu F.B.,Genetic Engineering and Biotechnology Institute | Zaghouani H.,University of Missouri
Immunology | Year: 2016

Effective humoral immunity ensues when antigen presentation by B cells culminates in productive cooperation with T lymphocytes. This collaboration, however, remains ill-defined because naive antigen-specific B cells are rare and difficult to track in vivo. Herein, we used a defined transfer model to examine how B lymphocytes, as antigen-presenting cells, shape the development of T-cell memory suitable for generation of relevant antibody responses. Specifically, we examined how B cells presenting different doses of antigen during the initial priming phase shape the development of CD4 T-cell memory and its influence on humoral immunity. The findings indicate that B cells presenting low dose of antigen favour the development of T helper type 1 (Th1) type memory, while those presenting a high antigen dose yielded better Th2 memory cells. The memory Th2 cells supported the production of antibodies by effector B cells and promoted isotype switching to IgG1. Moreover, among the B-cell subsets tested for induction of Th2 memory, the splenic but not peritoneal B220lo cells were most effective in sustaining Th2 memory development as well as immunoglobulin isotype switching, and this function involved a tight control by programmed death 1-programmed death ligand 2 interactions. © 2016 John Wiley & Sons Ltd. Source


Incesu Z.,Anadolu University | Hatipoglu I.,Genetic Engineering and Biotechnology Institute | Sivas H.,Anadolu University | Ergene E.,Anadolu University | Akalin Ciftci G.,Anadolu University
Indian Journal of Experimental Biology | Year: 2013

The aims of this study are the investigation of the effects of fibronectin and type IV collagen extracellular matrix proteins and the role of caspase-3 and-9 on cis-platin induced U2-OS apoptosis were studied. First the cytotoxic effects of cis-platin on cell system were investigated by colorimetric method and than morphological and ELISA analysis were used for determination of cell apoptosis when induced with cis-platin. In addition, after adhering the cells to fibronection or type IV collagen proteins, the apoptotic rate and the effects of caspase-3 and -9 were also investigated by ELISA in presence of specific inhibitors. U2-OS cells showed 20% cytotoxicity after treatment with 2.4 μM of cis-platin for 48 h. Morphological and the numerical data showed that cis-platin was able to induced apoptosis on cells as a dose-dependent manner. Caspase-3 and -9 inhibitors inhibited cis-platin-induced apoptosis in U2-OS cells, respectively. The binding of cells to 10 μg/mL of fibronectin but not type IV collagen enhanced the apoptosis about 2.5 fold that effects inhibited with caspase-3 inhibitor. The caspase-3 and -9 are involved in the apoptotic signals induced by cis-platin in U2-OS. The binding to fibronectin, but not type IV collagen enhanced the apoptotic response of U2-OS and fibronectin-dependent apoptosis was activated by caspase-3. These finding might be useful for patients to fight against osteosarcoma. Source

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