Akai H.,Yurtec Corporation |
Tochihara Y.,Kyushu University |
Ishimatsu S.,University of Occupational and Environmental Health Japan |
Kusakari H.,Yurtec Corporation |
And 4 more authors.
Journal of Environmental Engineering | Year: 2010
There are increasing reports of the evidence of Legionellosis. More rapid inspection of Legionella is necessary for minimizing damage to civilians. In this respect, on-site inspection method is required. This report provides the new method which can achieve on-site inspection of Legionella in water by combining the capture beads concentration and immunochromatography detection. In the concentration method, the beads which capture Legionella is used, and large volume sample (over 10 L) is treated by peristaltic pump. Beads concentration method can be also applicable for culture method and detection by polymerase chain reaction (PCR). The new method provides the practical solution for on-site legionella inspection.
Mitarai S.,Research Institute of Tuberculosis |
Karinaga R.,Genetein Co. |
Karinaga R.,Precision System Science Co. |
Yamada H.,Research Institute of Tuberculosis |
And 6 more authors.
Journal of Microbiological Methods | Year: 2012
Centrifugation is a necessary concentrating step for the detection of Mycobacterium tuberculosis in a liquid culture. However, centrifugation is biologically hazardous and presents an obstacle in the development of an automated culture system. A bead-based bacterial concentration method, TRICORE, was recently developed by Genetein Co., Ltd. We compared the efficacy of TRICORE and conventional centrifugation for concentrating M. tuberculosis in clinical sputum specimens by using liquid and solid culture systems. Among 90 pretreated clinical sputum specimens, 51 (57.3%) and 55 (61.8%) M. tuberculosis isolates were recovered by the MGIT culture system by using the centrifugation and TRICORE methods, respectively (chi-square test, p=. 0.5413). The detection time for the centrifugation method was 359.3 ± 117.0. h, while that for the bead-based concentration method was 377.6 ± 162.3. h (p=. 0.5637). However, the number of colonies recovered on solid media were significantly higher with the TRICORE method (p=. 0.003). In particular, among the smear-negative specimens, culture positivity of the TRICORE method was 39.6%, while that of the centrifugation method was 15.1%. The TRICORE bead-based concentration method was considered equivalent to centrifugation and enabled efficient collection of paucibacillary specimens in solution. Thus, the new noncentrifugation concentration method could yield more positive culture results. © 2012 Elsevier B.V..