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Tabata Y.,Next Generation Systems | Murai N.,Next Generation Systems | Sasaki T.,General Systems Research, LLC | Taniguchi S.,Next Generation Systems | And 3 more authors.
Journal of Biomolecular Screening | Year: 2015

Stem cell research has been progressing rapidly, contributing to regenerative biology and regenerative medicine. In this field, small-molecule compounds affecting stem cell proliferation/differentiation have been explored to understand stem cell biology and support regenerative medicine. In this study, we established a multiparametric screening system to detect bioactive compounds affecting the cell fate of human neural stem/progenitor cells (NSCs/NPCs), using human fetal hippocampal NSCs/NPCs, HIP-009 cells. We examined effects of 410 compounds, which were collected based on mechanisms of action (MOAs) and chemotypes, on HIP-009's cell fate (self-renewal, neuronal and astrocytic differentiation) and morphology by automated multiparametric assays and profiled induced cellular phenotypes. We found that this screening classified compounds with the same MOAs into subgroups according to additional pharmacological effects (e.g., mammalian target of rapamycin complex 1 [mTORC1] inhibitors and mTORC1/mTORC2 dual inhibitors among mTOR inhibitors). Moreover, it identified compounds that have off-target effects under matrix analyses of MOAs and structure similarities (e.g., neurotropic effects of amitriptyline among tri- and tetracyclic compounds). Therefore, this automated, medium-throughput and multiparametric screening system is useful for finding compounds that affect the cell fate of human NSCs/NPCs for supporting regenerative medicine and to fingerprint compounds based on human stem cells' multipotency, leading to understanding of stem cell biology. © 2015 Society for Laboratory Automation and Screening.

Tess A.,Beth Israel Deaconess Medical Center | Vidyarthi A.,General Systems Research, LLC | Yang J.,Beth Israel Deaconess Medical Center | Myers J.S.,University of Pennsylvania
Academic Medicine | Year: 2015

Integrating the quality and safety mission of teaching hospitals and graduate medical education (GME) is a necessary step to provide the next generation of physicians with the knowledge, skills, and attitudes they need to participate in health system improvement. Although many teaching hospital and health system leaders have made substantial efforts to improve the quality of patient care, few have fully included residents and fellows, who deliver a large portion of that care, in their efforts. Despite expectations related to the engagement of these trainees in health care quality improvement and patient safety outlined by the Accreditation Council for Graduate Medical Education in the Clinical Learning Environment Review program, a structure for approaching this integration has not been described. In this article, the authors present a framework that they hope will assist teaching hospitals in integrating residents and fellows into their quality and safety efforts and in fostering a positive clinical learning environment for education and patient care. The authors define the six essential elements of this framework - organizational culture, teaching hospital-GME alignment, infrastructure, curricular resources, faculty development, and interprofessional collaboration. They then describe the organizational characteristics required for each element and offer concrete strategies to achieve integration. This framework is meant to be a starting point for the development of robust national models of infrastructure, alignment, and collaboration between GME and health care quality and safety leaders at teaching hospitals. © 2015 by the Association of American Medical Colleges.

Rajagopal M.,Indiana University - Purdue University Indianapolis | Rajagopal M.,General Systems Research, LLC | Rajamanohar D.,Indian Institute of Technology Madras
Journal of Aerospace Engineering | Year: 2014

In this numerical study, the cooling of exhaust gas issued from a rocket engine in a ground test facility has been investigated by solving the three-dimensional governing equations. Simulations have been performed by employing discrete phase model with plain-orifice atomizer to cool the exhaust flow effectively by injecting the coolant (water) in the form of a fine spray. The effects of coolant flow rate, inlet gas temperature, injection pressure, injector diameter, gas cooler length, and distribution of water droplets on the cooling characteristics, such as the temperature at the gas cooler exit, droplet diameter, and percentage of unevaporated water have been discussed in detail. Simulations highlight that optimum particle diameter needs to be identified for effective cooling, and also uniform cooling of the exhaust gas is attained by employing staggered injector distribution (more injectors at the periphery compared to the core region). Predicted values of static temperature and pressure agree well with the experimental data obtained from a scaled-down model high-altitude test facility. © 2014 American Society of Civil Engineers.

Drake M.C.,General Systems Research, LLC
Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference: 2010 Laser Science to Photonic Applications, CLEO/QELS 2010 | Year: 2010

Imaging techniques (laser Mie scattering, particle image velocimetry, spectrally-resolved spark and combustion luminosity, and laser induced fluorescence) with high repetition rate lasers and multiple CMOS cameras allow fuel injection, fuel/air mixing, ignition, and combustion to be followed within one engine cycle. This aids the design of advanced gasoline direct injection engines by avoiding intermittent poor burning engine cycles. Improvements in tunable uv high repetition rate lasers would expand the range of possible in-cylinder experiments. ©2010 Optical Society of America.

Hicks S.,General Systems Research, LLC | Jones A.,University of Canterbury | Pennington A.,Heavy Engineering Research Association
Composite Construction in Steel and Concrete VII - Proceedings of the 2013 International Conference on Composite Construction in Steel and Concrete | Year: 2013

Composite slabs using cold-formed profiled steel sheeting have proved popular in multi-storey steel-framed buildings. This paper presents a comprehensive test programme that considers the performance of a modern trapezoidal profiled sheeting using Australasian 550 N/mm high strength steel, compared to that exhibited by the same profile using European 350 N/mm steel. Although the ductility of the Australasian steel is low, from composite slab tests it was found that the longitudinal shear behaviour could be considered ductile according to the Eurocode 4 requirements. As a consequence of this, the partial connection method was permitted. However, from the test results presented in this paper, it is shown that the rules given in Eurocode 4, Annex B.3.1 for a reduced investigation lead to unconservative results and should be revised. To remedy this situation, an amendment is proposed. © ASCE.

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