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Canadas M.P.,Retrovirology Laboratory IrsiCaixa | Canadas M.P.,General Laboratory | Videla S.,Lluita Contra La SIDA Foundation | Darwich L.,Retrovirology Laboratory IrsiCaixa | And 11 more authors.
Journal of Clinical Virology | Year: 2010

Background: Genomic integration of high-risk human papillomavirus into the cellular genome is considered an important event in the pathogenesis of cervical cancer related to the progression from premalignant cervical lesions to invasive cervical carcinoma. Objective: This cross-sectional study was aimed to characterize the viral integration of HPV-16, HPV-18, HPV-52 and HPV-58 in cervical cells. Study design: HPV genotypes were determined by PCR and HPV integration by multiplex PCR in HIV-1-infected women without a background of HPV-related pathology. Results: This study included 251 cervical cells samples of consecutive HIV-positive women who were visited between 1999 and 2003. The overall prevalence of HPV infection was 53% (133/251, 95%CI: 47-59%). The most prevalent genotypes were HPV-16 (27%), HPV-33 (15%), HPV-52 (8%) and HPV-58 (8%). The prevalence of abnormal cervical cytology was 33% (83/251, 95%CI: 27-39%). The overall prevalence of HPV integration was 11% (27/251, 95%CI: 7-15%), and the prevalence of HPV-16 integration was 33% (22/67, 95%CI: 22-45%), HPV-18 integration was 30% (3/10, 95%CI: 7-65%) and HPV-52 integration was 10% (2/19, 95%CI: 1-32%). No HPV-58 integration was detected. The percentage of HPV-16 and HPV-18 integration increased with the severity of the cervical lesions, HPV-16 integration was almost 70% and HPV-18 integration was 50% in high-grade squamous intraepithelial lesions. Integration was the most important risk factor associated with cervical dysplasia (OR = 30.6, 95%CI: 3.5-270.6). Conclusion: HPV integration might represent a good biomarker of the evolution from HPV infection to cervical cancer. Further prospective studies are required to validate our findings. © 2010 Elsevier B.V. All rights reserved.


This report studies General Laboratory Equipment in Global Market, especially in North America, Europe, China, Japan, Southeast Asia and India, focuses on top manufacturers in global market, with production, price, revenue and market share for each manufacturer, covering  Bruker  A&D Company, Limited  ADC Bioscientific  Bibby Scientific Limited   Bio-Rad Laboratories  Dispersion Technology Inc  Harvard Apparatus  Heidolph  Malvern Instruments  Merci   Michell Instruments  MSE (UK) ltd  Nanalysis  Polymer Characterization, S.A.  Sartorius AG  Scientifica Limited  Scilabware  Shimadzu Corporation  Syrris Ltd  Market Segment by Regions, this report splits Global into several key Regions, with production, consumption, revenue, market share and growth rate of General Laboratory Equipment in these regions, from 2011 to 2021 (forecast), like  North America  Europe  China  Japan  Southeast Asia  India  Split by product type, with production, revenue, price, market share and growth rate of each type, can be divided into  Glass product  Plastic products  General Laboratory instruments  Split by application, this report focuses on consumption, market share and growth rate of General Laboratory Equipment in each application, can be divided into  Pharmaceutical companies  Research Institute  University  Others Global General Laboratory Equipment Market Research Report 2016  1 General Laboratory Equipment Market Overview  1.1 Product Overview and Scope of General Laboratory Equipment  1.2 General Laboratory Equipment Segment by Type  1.2.1 Global Production Market Share of General Laboratory Equipment by Type in 2015  1.2.2 Glass product  1.2.3 Plastic products  1.2.4 General Laboratory instruments  1.3 General Laboratory Equipment Segment by Application  1.3.1 General Laboratory Equipment Consumption Market Share by Application in 2015  1.3.2 Pharmaceutical companies  1.3.3 Research Institute  1.3.4 University  1.3.5 Others  1.4 General Laboratory Equipment Market by Region  1.4.1 North America Status and Prospect (2011-2021)  1.4.2 Europe Status and Prospect (2011-2021)  1.4.3 China Status and Prospect (2011-2021)  1.4.4 Japan Status and Prospect (2011-2021)  1.4.5 Southeast Asia Status and Prospect (2011-2021)  1.4.6 India Status and Prospect (2011-2021)  1.5 Global Market Size (Value) of General Laboratory Equipment (2011-2021) 2 Global General Laboratory Equipment Market Competition by Manufacturers  2.1 Global General Laboratory Equipment Production and Share by Manufacturers (2015 and 2016)  2.2 Global General Laboratory Equipment Revenue and Share by Manufacturers (2015 and 2016)  2.3 Global General Laboratory Equipment Average Price by Manufacturers (2015 and 2016)  2.4 Manufacturers General Laboratory Equipment Manufacturing Base Distribution, Sales Area and Product Type  2.5 General Laboratory Equipment Market Competitive Situation and Trends  2.5.1 General Laboratory Equipment Market Concentration Rate  2.5.2 General Laboratory Equipment Market Share of Top 3 and Top 5 Manufacturers  2.5.3 Mergers & Acquisitions, Expansion 3 Global General Laboratory Equipment Production, Revenue (Value) by Region (2011-2016)  3.1 Global General Laboratory Equipment Production by Region (2011-2016)  3.2 Global General Laboratory Equipment Production Market Share by Region (2011-2016) 3.3 Global General Laboratory Equipment Revenue (Value) and Market Share by Region (2011-2016)  3.4 Global General Laboratory Equipment Production, Revenue, Price and Gross Margin (2011-2016)  3.5 North America General Laboratory Equipment Production, Revenue, Price and Gross Margin (2011-2016)  3.6 Europe General Laboratory Equipment Production, Revenue, Price and Gross Margin (2011-2016)  3.7 China General Laboratory Equipment Production, Revenue, Price and Gross Margin (2011-2016)  3.8 Japan General Laboratory Equipment Production, Revenue, Price and Gross Margin (2011-2016)  3.9 Southeast Asia General Laboratory Equipment Production, Revenue, Price and Gross Margin (2011-2016)  3.10 India General Laboratory Equipment Production, Revenue, Price and Gross Margin (2011-2016) For more information or any query mail at [email protected]


Canadas M.P.,Retrovirology Laboratory IrsiCaixa Foundation | Canadas M.P.,General Laboratory | Darwich L.,Retrovirology Laboratory IrsiCaixa Foundation | Darwich L.,Autonomous University of Barcelona | And 8 more authors.
Clinical Microbiology and Infection | Year: 2010

Human papillomavirus (HPV) infection is the cause of cervical cancer. Integration of HPV-16 DNA in cervical cells is considered to be a key event in the progression towards invasive cancer, but little is known about this event in anal carcinogenesis. The integration could be a useful biomarker for cancer progression. Optimized assays are needed to determine the value of real-time detection of HPV integration in longitudinal studies, and this approach is only possible with a high-throughput assay. The aim of this study was to develop a new multiplex real-time PCR assay based on simultaneous amplification of the E2 and E6 HPV open reading frames (ORFs) in order to assess the physical status (episomal and/or integrated) of HPV-16 in anal cells of HIV-positive men. The comparative threshold (Ct) cycle values for E2 and E6 obtained for SiHA cells and artificial mixtures of episomal and integrated DNA were as expected: similar Ct for episomal forms and absence of E2 amplification for integrated forms. The multiplex real-time PCR was tested in 77 consecutive samples from individual HIV-infected patients with HPV-16 anal infection. The integration of HPV-16 was detected in 25 (32%) patients: 23 as mixed (episomal and integrated) and two as completed integrated forms. The integration occurs in the early stage of anal lesions and was associated with the severity of the lesions (p 0.004). The multiplex real-time PCR assay developed in the course of this study was shown to be a simple, sensitive, specific and inexpensive technique which may be applied routinely to detect HPV-16 integration. © 2009 The Authors. Journal Compilation © 2009 European Society of Clinical Microbiology and Infectious Diseases.


Canadas M.-P.,Retrovirology Laboratory IrsiCaixa Foundation | Canadas M.-P.,General Laboratory | Darwich L.,Retrovirology Laboratory IrsiCaixa Foundation | Darwich L.,Autonomous University of Barcelona | And 12 more authors.
Sexually Transmitted Diseases | Year: 2010

Background: The integration of HPV-16 DNA into the host genome is considered an important event in the progression of premalignant cervical lesions to cervical cancer. The aim of our study was to assess the prevalence of HPV-16 integration in anal cytologic specimens of HIV-1 infected men and its association with risk factors. Patients methods: This cross-sectional study included 269 HIV-infected males. Detection and typing of HPV-infection was done by multiplex PCR, and integration of HPV-16 by real-time PCR. Results: The overall anal HPV-infection prevalence was 78% (209/269), 29% (77/269) for HPV-16 infection, and 9% (25/269) for HPV-16 integration. In HPV-16 infected group, the integration prevalence represented 32% (25/77). The only risk factor associated with HPV-16 integration was the time since HIV diagnosis (OR = 1.2, 95% CI: 1.0-1.3; P = 0.010). The risk factors associated with abnormal cytology results were: HPV infection (OR = 17.8, 95% CI: 6.8-46.6), HPV-16 infection (OR = 4.6, 95% CI: 2.5-8.4), and presence of HPV-16 integrated forms (OR = 11.7, 95% CI: 1.5-93.5). Moreover, in the multivariate analysis, the HPV-16 integration continued representing the most important risk factor (OR = 20, 95% CI: 1.6-226) for anal cytologic abnormalities. Conclusion: HPV-16 infection and its integration in anal cells were highly prevalent in HIV-infected men. The assessment of HPV-16 integration rather than HPV-infection could be a good biomarker for predicting anal precancerous lesions in HIV-positive men. Copyright © 2010 American Sexually Transmitted Diseases Association All rights reserved.


Ruibal A.,University of Santiago de Compostela | Aguiar P.,University of Santiago de Compostela | Del Rio M.C.,General Laboratory | Padin-Iruegas M.E.,University of Vigo | And 2 more authors.
International Journal of Molecular Sciences | Year: 2014

Breast cancer is currently becoming a disease of the elderly. We have studied the relation between CA 15.3 serum concentrations and clinical-pathological parameters in 69 women with IDC aged over 70 years (76.3 ± 4.2; range: 71-88; median 76). A group of 205 women with the same tumor but aged <70 years (62.8 ± 4.0; range: 55-70; median 63) was also considered for comparison. Tumor size, axillary lymph node involvement, distant metastasis and histological grade were taken account. Serum CA 15.3 was determined by luminescence assay. CA 15.3 serum concentrations ranged between 6 and 85 U/mL (median 22.9 U/mL), and were higher only in patients with greater (qualitative and quantitative; p: 0.041) tumor size. Our results show that in women with IDCs, and aged over 70 years, serum CA 15.3 serum concentrations are associated exclusively with a  greater tumor size, being these findings different to those described in women with the same subtype of tumor considered as a whole or with lower age. © 2014 by the authors; licensee MDPI, Basel, Switzerland.


PubMed | General Laboratory, University of Vigo, University of Santiago de Compostela and Hospital of Monte Naranco
Type: Journal Article | Journal: International journal of molecular sciences | Year: 2014

Breast cancer is currently becoming a disease of the elderly. We have studied the relation between CA 15.3 serum concentrations and clinical-pathological parameters in 69 women with IDC aged over 70 years (76.34.2; range: 71-88; median 76). A group of 205 women with the same tumor but aged <70 years (62.84.0; range: 55-70; median 63) was also considered for comparison. Tumor size, axillary lymph node involvement, distant metastasis and histological grade were taken account. Serum CA 15.3 was determined by luminescence assay. CA 15.3 serum concentrations ranged between 6 and 85 U/mL (median 22.9 U/mL), and were higher only in patients with greater (qualitative and quantitative; p: 0.041) tumor size. Our results show that in women with IDCs, and aged over 70 years, serum CA 15.3 serum concentrations are associated exclusively with a greater tumor size, being these findings different to those described in women with the same subtype of tumor considered as a whole or with lower age.


Montiel R.,CINVESTAV | Montiel R.,University of The Azores | Solorzano E.,Autonomous University of Barcelona | Diaz N.,Autonomous University of Barcelona | And 7 more authors.
PLoS ONE | Year: 2012

Ancient DNA (aDNA) analysis can be a useful tool in bacterial disease diagnosis in human remains. However, while the recovery of Mycobacterium spp. has been widely successful, several authors report unsuccessful results regarding ancient treponemal DNA, casting doubts on the usefulness of this technique for the diagnosis of ancient syphilis. Here, we present results from an analysis of four newborn specimens recovered from the crypt of "La Ermita de la Soledad" (XVI-XVII centuries), located in the province of Huelva in the southwest of Spain. We extracted and analyzed aDNA in three independent laboratories, following specific procedures generally practiced in the aDNA field, including cloning of the amplified DNA fragments and sequencing of several clones. This is the most ancient case, reported to date, from which detection of DNA from T. pallidum subspecies pallidum has been successful in more than one individual, and we put forward a hypothesis to explain this result, taking into account the course of the disease in neonate individuals. © 2012 Montiel et al.


Canadas M.P.,Lluita Contra La SIDA Foundation | Canadas M.P.,General Laboratory | Darwich L.,Lluita Contra La SIDA Foundation | Darwich L.,Autonomous University of Barcelona | And 7 more authors.
Clinical Microbiology and Infection | Year: 2013

Male circumcision is associated with a lower risk of penile human papillomavirus (HPV) infection in human immunodeficiency virus (HIV) uninfected men. Few studies have evaluated the role of male circumcision in penile HPV infection in HIV-infected men. The aim of this cross-sectional study was to examine the association between male circumcision and the prevalence of penile HPV infection among HIV-infected men-both men who have sex with men (MSM) and heterosexual men. Samples from 706 consecutive men included in the CARH-MEN cohort (overall 24% circumcised: 26% of MSM, 18% of heterosexual men) were examined by Multiplex-PCR. In the overall group (all HIV-infected men included), the prevalence of any penile HPV infection was 22% in circumcised men and 27% in uncircumcised men (OR=1.0, 95% CI 0.6-1.6, adjusted analysis). In the circumcised group the overall prevalence of HPV infection was 22% in MSM and 24% in the heterosexual men, whereas in the uncircumcised group the prevalence was 26% and 28%, respectively. The prevalence of high-risk HPV types tended to be lower in the circumcised MSM (14% vs 21%, OR=0.6, 95% CI 0.3-1.1, p0.088), but it was similar in the heterosexual men (18% in circumcised vs 20% in uncircumcised). These results suggest that male circumcision may be associated with a lower prevalence of oncogenic high-risk penile HPV infection in HIV-infected MSM. © 2012 European Society of Clinical Microbiology and Infectious Diseases.

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