General Hospital of the Yangtze River Shipping

Wuhan, China

General Hospital of the Yangtze River Shipping

Wuhan, China
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Wan Y.,New York State Department of Health | Wan Y.,General Hospital of the Yangtze River Shipping | Xue J.,New York State Department of Health | Kannan K.,New York State Department of Health | Kannan K.,King Abdulaziz University
Science of the Total Environment | Year: 2015

Benzophenone-3 (BP-3) is a widespread environmental contaminant and an estrogenic compound. Very little is known with regard to the occurrence in indoor air and the inhalation exposure of humans to BP-3. In this study, 81 indoor air samples were collected from various locations in Albany, New York, USA, in 2014 and analyzed for BP-3 by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). BP-3 was found in all indoor air samples and the overall concentrations in bulk air (vapor plus particulate phases) were in the range of 0.19-72.0ng/m3 (geometric mean: 2.67ng/m3). The highest concentrations (geometric mean: 10.7ng/m3) were found in cars, followed by barber shops (6.57) > public places (5.75)>homes (3.27) > offices (1.96) > garages (1.04) > laboratories (0.47). The estimated geometric mean daily intake (EDI) of BP-3 for infants, toddlers, children, teenagers, and adults through indoor air inhalation from homes was 1.83, 1.74, 1.18, 0.69, and 0.51ng/kg-bw/day, respectively. Although high concentrations of BP-3 were measured in some microenvironments, the estimated contribution of indoor air to total BP-3 intake was <5% of the total BP-3 intake in humans. This is the first survey on the occurrence of BP-3 in indoor air. © 2015 Elsevier B.V.


Xue J.,New York State Department of Health | Wan Y.,General Hospital of the Yangtze River Shipping | Kannan K.,New York State Department of Health | Kannan K.,King Abdulaziz University
Chemosphere | Year: 2016

Bisphenols, bisphenol A diglycidyl ethers (BADGEs), and novolac glycidyl ethers (NOGEs) are used in the production of epoxy resins and polycarbonate plastics. Despite the widespread application of these chemicals in household products, studies on their occurrence in indoor air are limited. In this study, 83 indoor air samples were collected in 2014 from various locations in Albany, New York, USA, to determine the concentrations of bisphenols, BADGEs (refer to BADGE and its derivatives), and NOGEs (refer to NOGE and its derivatives) and to calculate inhalation exposure to these compounds. Among eight bisphenols measured, BPA, BPF, and BPS were found in bulk air (i.e., vapor plus particulate phases), at geometric mean (GM) concentrations of 0.43, 0.69 and 0.09 ng m-3, respectively. Among 11 BADGEs and NOGEs determined, BADGE·2H2O was the predominant compound in indoor air (detection rate [DR]: 85.5%), at concentrations as high as 6.71 ng m-3. Estimation of inhalation exposure to these chemicals for various age groups showed that teenagers had the highest exposure doses to BPA, BPF, BPS, and BADGE·2H2O at 5.91, 9.48, 1.24, and 3.84 ng day-1, respectively. The body weight-normalized estimates of exposure were the highest for infants, with values at 0.24, 0.39, 0.05, and 0.16 ng kg bw-1 day-1 for BPA, BPF, BPS, and BADGE·2H2O, respectively. This is the first survey to report inhalation exposure to bisphenols, BADGEs, and NOGEs. © 2016 Elsevier Ltd.


Peng J.-J.,General Hospital of the Yangtze River Shipping | Wu B.,The First Hospital of Wuhan City | Xiao X.-B.,The First Hospital of Wuhan City | Shao Y.-S.,The First Hospital of Wuhan City | And 2 more authors.
Archives of Medical Research | Year: 2014

Background and Aims: To investigate the expression and prognostic significance of Krüppel-like factor 17 (KLF17) in human gastric cancer. Methods: KLF17 expressions in 158 paraffin-embedded gastric cancer samples were analyzed using immunohistochemistry. In addition, KLF17 expressions patterns in three fresh gastric cancer tissues and noncancerous gastric mucosa were examined by Western blotting. The correlation between KLF17 expression and clinicopathological factors as well as patient survival was investigated. Results: Immunohistochemical staining data indicated that KLF17 expression was significantly decreased in 98 of 158 gastric adenocarcinoma cases. Reduced KLF17 expression in fresh gastric cancer tissues was confirmed by Western blotting. Reduced expression of KLF17 was strongly correlated with tumor size, pN stage and lymphovascular invasion. Multivariate Cox regression analysis identified KLF17 expression as an independent prognostic factor for both overall survival (HR = 0.481, 95% CI = 0.225-0.665, p = 0.009) and disease-free survival (HR = 0.438, 95% CI = 0.254-0.758, p = 0.003). Conclusion: The reduced expression of KLF17 protein in gastric cancer was correlated with tumor size, pN stage and lymphovascular invasion and was an independent predictor for poor survival in patients undergoing surgery for gastric cancer. © 2014 IMSS.


Huang X.-F.,General Hospital of the Yangtze River Shipping | Yuan S.-J.,General Hospital of the Yangtze River Shipping | Yang C.,Huazhong University of Science and Technology
Molecular Medicine Reports | Year: 2012

Dental pulp stem cells (DPSCs) have the potential to form bone, nerve and fat, and are a candidate for use in regenerative medicine. Previous studies indicated that total flavonoids from Drynaria fortunei show a stimulative effect on the proliferation and osteogenic differentiation of osteoblastic MC3T3-E1 cells in vitro. This study aimed to investigate the effect of total flavonoids from Drynaria fortunei on the proliferation and osteogenic differentiation of rat DPSCs, and to further clarify the mechanisms involved. DPSCs were isolated by enzymatic digestion and identified using the CD44, CD29 and CD34 markers by immunohistochemistry, and exposed to 0.01, 0.05 and 0.1 g/l total flavonoids from Drynaria fortunei media. Total flavonoids from Drynaria fortunei promoted the proliferation of DPSCs in a dose-dependent manner and this effect may depend on the shortening of the G0/G1 phase and promotion of the S phase. Compared with the control group, the levels of alkaline phosphatase (ALP) and the expression of osteogenic genes increased with the concentrations of total flavonoids from Drynaria fortunei, and the volume and number of calcified nodules in the Drynaria groups was bigger compared to the control group. These results suggest that total flavonoid from Drynaria fortunei directly stimulates DPSC proliferation and osteogenic differentiation, and may serve as a new promising candidate drug for dental tissue engineering and bone regeneration.


Pu F.,Huazhong University of Science and Technology | Chen F.,General Hospital of The Yangtze River Shipping | Chen S.,Huazhong University of Science and Technology | Wang B.,Huazhong University of Science and Technology | And 2 more authors.
OncoTargets and Therapy | Year: 2015

Objective: The aim of this study was to evaluate the relationship between GSTP1 polymorphisms and prognosis of osteosarcoma in patients treated with chemotherapy, by performing a meta-analysis. Methods: The studies of effects of GSTP1 gene polymorphisms on osteosarcoma survival after chemotherapy were collected. STATA (version 12.0) was used to perform data synthesis. Results: Six studies involving 898 participants were included. A meta-analysis was performed on studies in GSTP1 313A>G(rs1695) assessing the association between tumor response and the polymorphisms in GSTP1 (AA vs AG, AA vs GG), the pooled odds ratios (ORs) were 2.06 (95% confidence interval [CI]: 1.48–2.86, P=0.628, I2=0.0%). There was significant association between the polymorphisms in GSTP1 (AA vs AG, AA vs GG) and the events that happened, the pooled ORs were 1.86 (95% CI: 1.14–3.06, P=0.034, I2=58.6%), and there was significant association between the polymorphisms in GSTP1 (AA vs AG, AA vs GG) and survival times (overall survival and progression-free survival) in osteosarcoma patients treated with chemotherapy, and the pooled ORs were 2.14 (95% CI: 1.51–3.04, P=0.675, I2=0.0%) and 2.77 (95% CI: 1.56–4.91, P=0.347, I2=9.3%), respectively. Two studies assessed the association of polymorphisms in GSTP1 I105V (IIe/IIe vs IIe/Val, IIe/IIe vs Val/Val) with overall survival in human osteosarcoma. The pooled ORs were 1.20 (95% CI: 0.64–2.27, P=0.010, I2=73.5%). The study showed an insignificant difference in overall survival for the polymorphisms in GSTP1 (IIe/IIe vs IIe/Val, IIe/IIe vs Val/Val). Conclusion: This meta-analysis indicated that GSTP1 polymorphisms might influence osteosarcoma risk and suggests that GSTP1 polymorphisms may be an important risk factor for osteosarcoma. © 2015 Pu et al.


Wang B.,Huazhong University of Science and Technology | Wu Y.,Huazhong University of Science and Technology | Shao Z.,Huazhong University of Science and Technology | Yang S.,Huazhong University of Science and Technology | And 4 more authors.
Journal of Biomedical Materials Research - Part A | Year: 2012

In this study, a new functionalized peptide RLN was designed containing the bioactive motif link N, the amino terminal peptide of link protein. A link N nanofiber scaffold (LN-NS) was self-assembled by mixing peptide solution of RLN and RADA16. The characterization of LN-NS was tested using atomic force microscopy (AFM). The biocompatibility and bioactivity of this nanofiber scaffold for rabbit nucleus pulposus cells (NPCs) were also evaluated. This designer functionalized nanofiber scaffold exhibited little cytotoxicity and promoted NPCs adhesion obviously. In three-dimensional cell culture experiments, confocal reconstructed images testified that the functionalized LN-NS-guided NPCs migration from the surface into the hydrogel considerably, in which the RADA16 scaffold did not. Moreover, the functionalized LN-NS significantly stimulated the biosynthesis of extracelluar matrices (ECM) by NPCs. Our findings demonstrate that the functionalized nanofiber scaffold containing link N had excellent biocompatibility and bioactivity with rabbit NPCs and could be useful in the nucleus pulposus regeneration. Copyright © 2011 Wiley Periodicals, Inc.


Pu F.,Huazhong University of Science and Technology | Chen F.,General Hospital of The Yangtze River Shipping | Shao Z.,Huazhong University of Science and Technology
Tumor Biology | Year: 2016

MicroRNAs are a group of small non-coding RNAs that play a complex role in post-transcriptional gene expression and can be used for diagnosis, prognosis, and targeted treatment. Despite advances in diagnosis and treatment of chondrosarcoma, its underpinning molecular mechanisms still remain elusive. Given the recent increasing knowledge base of micro RNA (miRNA) roles in neoplasia, both as oncogenes and tumor suppressor genes, this review will focus on discussing the available data on expression profiles and potential roles of miRNA in chondrosarcoma. Accumulating evidence suggests that microRNAs have the potential to be used in the future for clinical management of chondrosarcoma. © 2016 International Society of Oncology and BioMarkers (ISOBM)


PubMed | General Hospital of The Yangtze River Shipping and Huazhong University of Science and Technology
Type: | Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | Year: 2016

MicroRNAs are a group of small non-coding RNAs that play a complex role in post-transcriptional gene expression and can be used for diagnosis, prognosis, and targeted treatment. Despite advances in diagnosis and treatment of chondrosarcoma, its underpinning molecular mechanisms still remain elusive. Given the recent increasing knowledge base of micro RNA (miRNA) roles in neoplasia, both as oncogenes and tumor suppressor genes, this review will focus on discussing the available data on expression profiles and potential roles of miRNA in chondrosarcoma. Accumulating evidence suggests that microRNAs have the potential to be used in the future for clinical management of chondrosarcoma.


PubMed | General Hospital of The Yangtze River Shipping and Huazhong University of Science and Technology
Type: | Journal: OncoTargets and therapy | Year: 2016

Oldenlandia diffusa (OD) is a well-known traditional Chinese medicine, which is used to prevent and treat many disorders, especially cancers. However, its role in osteosarcoma has not been well understood. Here, we used OD and cisplatin individually and combined in osteosarcoma MG-63 cell to explore whether OD could induce cellular apoptosis and suppress the ability of proliferation and invasion of osteosarcoma MG-63 cell.The changes of cellular shape were analyzed by optical microscopy. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay was used to analyze cell survival rate in vitro. Flow cytometry was performed to detect cell cycle and cell death. Scratch migration assay was used to evaluate cell migration and invasion. Western blot was performed to determine the expression levels of pro-apoptotic and anti-apoptotic protein.In this study, we found that the survival rate reduced significantly in the combined group compared with the individual group and control group. The apoptosis-inducing effect of combined application was much more significant than that of individual application. The invasion ability of combined application was significantly lower than that of the individual application. In the combined group, there were high expression levels of pro-apoptotic protein and low expression of anti-apoptotic protein. Cell-cycle analysis showed a change in the cell-cycle distribution and arrested cells in G2-M phase.In this study, we found that OD inhibited proliferation and induced apoptosis in the human osteosarcoma MG-63 cell line in a time-dependent and dose-dependent manner. In addition, OD displayed inhibitory activity on MG-63 cell proliferation and invasion and the study also showed that OD activity might be mediated by caspase activation. These data suggest that OD might represent a novel, efficient candidate agent for further experimentation in osteosarcoma treatment.


PubMed | General Hospital of The Yangtze River Shipping
Type: Journal Article | Journal: Oncology research and treatment | Year: 2016

Bone metastases lead to local bone destruction and skeletal complications. Bisphosphonates, particlulaly zoledronic acid (ZA), play a central role in the treatment of bone metastases. Some studies have shown that denosumab may delay and prevent SREs in metastatic bone disease more effectively than ZA; therefore, we systematically reviewed and assessed the safety of denosumab and ZA.The PubMed, EMBASE, Cochrane Library, Web of Science with Conference Proceedings, Elsevier, and China National Knowledge Infrastructure (CNKI) databases were searched up to October 2015. 2 independent reviewers extracted data from each eligible study using a standard protocol, and both fixed-effects and random-effects models were used to analyze and evaluate the data extracted from eligible articles.6 randomized controlled trials enrolling 13,733 patients were included. Occurrences of adverse events were generally similar between the denosumab and ZA groups except anemia and anorexia in patients with bone metastases and back pain and bone pain. However, occurrences of serious adverse events such as hypocalcaemia , renal adverse events , and new primary malignancy were significantly different between the denosumab and ZA groups. Only the occurrence of osteonecrosis of the jaw showed no significant difference between the denosumab and ZA groups in patients with bone metastases.Denosumab was safer in delaying or preventing skeletal-related events in patients with bone metastases and prevented pain progression compared to ZA in this meta-analysis.

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