General Hospital of the second Artillery Force

Beijing, China

General Hospital of the second Artillery Force

Beijing, China

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Xu W.,Peking Union Medical College | Sun J.,Peking Union Medical College | Wang W.,Peking University | Wang X.,General Hospital of the second Artillery Force | And 14 more authors.
Hormones | Year: 2014

Objective: To determine if GC (group-specific component globulin) and CYP2R1 genetic variants have an association with serum 25-OHD3 levels, BMD or bone turnover markers in a population of Chinese postmenopausal women. Design: We randomly selected 1494 postmenopausal women of the Han ethnic group from seven communities in Beijing. BMD was determined by dual energy X-ray absorptiometry; serum bone turnover markers and 25-OHD3 were measured by the automated Roche electrochemiluminescence system; genotypes of GC and CYP2R1 were detected by the TaqMan allelic discrimination assay. Multiple statistic methods were used to test the associations of SNP genotypes and vitamin D levels. Results: In our sample, 89.6% women had vitamin D deficiency and another 9.8% had vitamin D insufficiency. The variants of rs2298849 (β=0.105, P<0.001) in GC were significantly associated with serum 25-OHD3 levels. Allele G of rs2298849 might be protective for serum 25-OHD3 level. Among the haplotypes of rs222020-rs2298849, CG (β=0.104, P=0.001) corresponded to increasing serum 25-OHD3concentrations. CYP2R1 polymorphisms showed some significant association with serum β-CTX and P1NP levels. Conclusions: We found that GC variants had a significant association with serum 25-OHD3 levels among postmenopausal women of the Han ethnic group in Beijing, while CYP2R1 variants were not found to be significant.

Zhao J.,Peking Union Medical College | Xia W.,Peking Union Medical College | Nie M.,Peking Union Medical College | Zheng X.,China Rehabilitation Research Center | And 24 more authors.
Bone | Year: 2012

The Matrilin3 gene (MATN3) encodes an extracellular matrix protein, which modulates chondrocyte differentiation. The aim of this study was to test for association of MATN3 polymorphisms with bone mineral density (BMD), fracture, vertebral fracture, bone turnover or 25-hydroxyvitamin D [25(OH)D] in postmenopausal women. A community-based population of 1488 postmenopausal women was randomly selected in Beijing. The history of fracture and vertebral fracture was obtained via questionnaire and vertebral X-ray respectively. BMD of lumbar spine (2-4), femoral neck and total hip were measured by dual energy X-ray absorptiometry. Serum N-terminal procollagen of type 1 collagen (P1NP), β-isomerized type I collagen C-telopeptide breakdown products (β-CTX) and 25(OH)D were quantified. Binary logistic regression revealed that Haplotype-4 was significantly associated with vertebral fracture risk in both additive model (p = 0.023, OR = 1.521) and dominant model (p = 0.028, OR = 1.623). The significance remained after 10,000 permutation tests to correct multiple testing (p = 0.042). Re-selected age matched vertebral fracture case-control groups revealed similar associations in additive model (p = 0.014, OR = 1.927, 95%CI = 1.142-3.253) and in dominant model (p = 0.011, OR = 2.231, 95%CI = 1.200-4.148). However, no significant association was found between MATN3 polymorphisms and serum β-CTX, P1NP, 25(OH)D levels, or BMD. In linear regression, Haplotype-2 approached marginal significance in association with femoral neck BMD T-score (p = 0.050), but this would account for only 0.2% of BMD variation in our sample. This study suggests that Haplotype-4 of MATN3 is associated with vertebral fracture risk independent of BMD in Chinese postmenopausal women. Efforts should be made to replicate our finding in other, similar and ethnically diverse, populations. © 2011 Elsevier Inc.

Hu X.,Beijing Normal University | Hu X.,Universities Confederated Institute of Proteomics | Hu X.,General Hospital of the Second Artillery Force | Zhang P.,Beijing Normal University | And 10 more authors.
Journal of International Medical Research | Year: 2012

Objective: The early diagnosis of nonfunctioning pituitary adenoma (NFPA) is difficult. The objective of this study was to find specific protein biomarkers to aid in the early detection of NFPA. Methods: Serum samples from 34 patients with NFPA and 34 age- and sex-matched healthy control subjects were analysed using surface-enhanced laser desorption/ ionization time-of-flight mass spectrometry (SELDI-TOF-MS) technology. The spectra were generated, protein peak clustering was performed and classification analyses were carried out using a decision tree classification algorithm. Results: Nine differentially expressed serum proteins were identified in the patients with NFPA compared with the control subjects. Both the sensitivity and specificity of the decision tree classification algorithm were 82.4% for NFPA. Conclusion: Nine new serum protein biomarkers for NFPA were identified. SELDI-TOF-MS coupled with data mining tools might provide a novel approach for the early diagnosis of NFPA and population screening for the disease. © 2012 Field House Publishing LLP.

Zhao J.,Peking Union Medical College | Xia W.,Peking Union Medical College | Nie M.,Peking Union Medical College | Zheng X.,China Rehabilitation Research Center | And 23 more authors.
Menopause | Year: 2011

Objective: The aim of this study was to evaluate serum N-aminoterminal propeptide of type I collagen (P1NP), C-terminal telopeptide of type I collagen (β-CTX), and vitamin D status in healthy Chinese postmenopausal women. The study was also designed to investigate their possible relationships with osteoporosis phenotypes. Methods: A community-based population of 1,724 postmenopausal women in Beijing was randomly selected. Serum bone turnover markers and 25-hydroxyvitamin D [25(OH)D] were tested by an automated Roche electrochemiluminescence system. Dual-energy X-ray absorptiometry was used to measure bone mineral density (BMD). Results: The mean (SD) values of serum β-CTX and P1NP were 0.439 (0.210) and 56.7 (27.9) ng/mL, respectively. The 25(OH)D level of postmenopausal women in Beijing was remarkably low (13.2 ± 5.4 ng/mL). Serum β-CTX and P1NP levels were negatively correlated with BMDs of lumbar spine, femoral neck, and total hip (all P < 0.01). The cubic regression model better fitted the relationships of BMD and bone turnover markers. Serum β-CTX levels were significantly higher in women with sustained osteoporotic fracture or vertebral fracture (P = 0.006 and 0.012, respectively). No association between P1NP and fracture or vertebral fracture was detected. The same situation applied to 25(OH)D. 25(OH)D was negatively correlated with β-CTX and P1NP (r =-0.073 and-0.088, P = 0.002 and <0.001, respectively). Conclusions: Serum β-CTX and P1NP levels were negatively correlated with BMD. β-CTX was significantly higher in postmenopausal women with sustained fracture or vertebral fracture. Vitamin D deficiency was highly prevalent in postmenopausal women in Beijing. © 2011 The North American Menopause Society.

Li N.,Chinese PLA General Hospital | Li N.,Peking Union Medical College | Wang X.,General Hospital of the Second Artillery Force | Jiang Y.,Peking Union Medical College | And 16 more authors.
Menopause | Year: 2014

OBJECTIVE: GALNT3 gene encodes the glycosyltransferase polypeptide N-acetylgalactosaminyltransferase-3 (ppGalNacT3), which initiates the O-glycosylation of fibroblast growth factor 23 (FGF23) that is important in phosphorous regulation. Inactivating mutations of the GALNT3 gene can cause familial tumoral calcinosis. The aim of present study is to investigate the association of GALNT3 polymorphisms with osteoporosis phenotypes in Chinese postmenopausal women. METHODS: A community-based population of 1,353 postmenopausal women was randomly selected in Beijing. Bone mineral densities (BMDs) of the lumbar spine, femoral neck (FN), and total hip (TH) were measured by dual-energy X-ray absorptiometry. Vertebral fracture phenotypes were ascertained by vertebral X-ray reading. Osteoporotic fracture phenotypes were obtained from questionnaires. Single nucleotide polymorphisms of GALNT3 were determined by TaqMan allelic discrimination assay. Differences in BMD, serum phosphorus, or serum calcium across diverse genotypes or haplotypes were analyzed by general linear model analysis of covariance. Linear regression or logistic regression was used for association analyses of different osteoporosis phenotypes, phosphorous, or calcium. Partial correlation was used to investigate the relationship between phosphorus or calcium and BMD. RESULTS: We found that polymorphisms of rs1863196, rs6710518, and rs13429321 were significantly associated with FN BMD (P values of 0.002, 0.003, and 0.002, respectively). Polymorphisms of rs1863196, rs6710518, rs4667492, rs13429321, and rs6721582 were associated with TH BMD (P values of 0.002, 0.004, 0.037, 0.005, and 0.014, respectively). Haplotype-1 additive and dominant models were found to be associated with TH BMD (P values of 0.035 and 0.024, respectively). Haplotype-2 dominant model was found to be associated with FN BMD (P = 0.003) and TH BMD (P = 0.001). CONCLUSIONS: GALNT3 may play a role in genetic susceptibility to osteoporosis among Chinese postmenopausal women. Efforts should be exerted to replicate our findings in other similar and ethnically diverse populations. © 2014 The North American Menopause Society.

Li P.,PLA Fourth Military Medical University | Li P.,Southern Medical University | Li P.,Chinese Institute of Basic Medical Sciences | Zhang Y.,General Hospital of the Second Artillery Force | And 6 more authors.
Molecular Biology Reports | Year: 2012

In this article we report on the culturing of dental enamel organ epithelia (EOE) using a rotary cell culture system (RCCS) bioreactor associated with a cytodex-3 microcarrier. This culture system enhanced the proliferation and differentiation of the EOE into ameloblasts. Primary dental EOE trypsinized from 4-day old post-natal rat pups were cultured in the RCCS associated with Cytodex-3. The results were analyzed in comparison to a conventional plate system (control). Cells grown in RCCS have shown higher viabilities (above 90%) and final cell densities in terms of cells/ml than in the control system. In the case of RCCS, 46 ± 2 manifold increases were obtained, while significantly lower yields of 10.8 ± 2.5 manifod were obtained for control plates. Throughout the experiments, glucose levels were maintained within the accepted physiological range. In this case, LDH levels are kept low (below 150 mmol/ml), which is in accordance with the low cell death observed in the RCCS. Scanning electron microscopy revealed cells that were spread and forming three dimensional aggregates on the surface of cytodex-3. Cells cultured in the RCCS exhibited a stronger positive immunofluorescence staining for ameloblastin than those in control plates. RT-PCR results revealed that cells cultured in RCCS have higher amelogenin mRNA levels compared to controls. We have done an exploratory study on biological characteristics and self-assembling of epithelium cellula intersitialis, which demonstrated that the special 3D environment enhanced the rat dental EOE cell proliferation and differentiation into ameloblasts. The study has revealed that RCCS could be used to study the reaction of the EOE cells, tooth enamel organ cells and mesenchymal cells under the spacial 3D culture system, which will also provide a novel hypothesis for dental regeneration. © 2011 Springer Science+Business Media B.V.

Yang B.,PLA General Hospital | Liu H.,PLA General Hospital | Zhang L.-Y.,General Hospital of the Second Artillery Force | Li J.-Y.,PLA General Hospital | And 3 more authors.
Acta Academiae Medicinae Sinicae | Year: 2010

Objective: To investigate the antitumor effect of natural killer (NK) cells on human colorectal cancer cells HT-29 in vitro by blocking transforming growth factor-β (TGF-β) signaling in NK cells transfected with vector containing dominant negative TGF-β type II receptor (DNTβR II). Methods: TGF-β1 was added at the final concentration of 10ng/ml for HT-29 cells. Primary NK cells were transfected with recombinant plasmid pIRES2-AcGFP-DNTβR II and control plasmid pIRES2-AcGFP using Amaxa Nucleofector technology respectively. The cytotoxicity of these two types of NK cells to HT-29 cells was detected and analyzed by cell counting kit-8. Results: The transfection efficiency of primary NK cells was 18.85% for the plasmid pIRES2-AcGFP-DNTβR II and 35.28% for the control plasmid pIRES2-AcGFP. The expression of DNTβR II in NK cells was confirmed by Western blotting and RT-PCR. Primary NK cells displayed significantly lower cytotoxity against HT-29 cells incubated with TGF-β1 than that without TGF-β1 (effect-target cell ratio 10:1, 14.40% ± 2.00% vs. 26.14% ± 2.50%, P < 0.05; effect-target cell ratio 20:1, 19.18% ± 2.49% vs. 40.81% ± 3.50%, P < 0.05). The cytotoxity of NK cells transfected with DNTβR II vector was significantly higher than that with control vector against HT-29 cells cultured with 10ng/ml TGF-β1 (effect-target cell ratio 10:1, 21.17% ± 2.49% vs. 11.48% ± 1.11%, P < 0.05; and effect-target cell ratio 20:1, 35.30% ± 3.78% vs. 17.19% ± 2.29%, P < 0.05). Conclusion: NK cells transfected with DNTβR II vector show better antitumor effect, which may provide new method for NK-based adoptive immunotherapy for cancer.

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