Chen W.,Chongqing Medical University |
Jiang Y.,Chongqing Medical University |
Han J.,Chongqing Medical University |
Hu J.,Chongqing Medical University |
And 8 more authors.
FEBS Journal | Year: 2017
Abnormal lipid metabolism, renal lipid accumulation and lipotoxicity are associated with the pathological features of glomerulopathy. However, the mechanisms by which lipid accumulation leads to the development or progression of this disease have not been fully elucidated. In this work, we have identified a role for the rate-limiting enzyme in lipolysis, adipose triglyceride lipase (ATGL; also called patatin-like phospholipase domain-containing protein 2), in renal lipid metabolism and kidney disease. ATGL-deficient (Atgl(−/−)) mice displayed albuminuria, accompanied by ectopic deposition of fat in the kidney. Magnetic resonance imaging demonstrated that the contrast agent gadopentetic acid was retained in kidney tissue, suggesting defects in the glomerular filtration barrier. Furthermore, transmission electron microscopy revealed lipid deposits in the podocyte, along with foot process fusion and morphological changes suggestive of apoptosis. Indeed, shRNA-mediated depletion of ATGL promoted podocyte apoptosis, accompanied by increased levels of intracellular reactive oxygen species (ROS) and F-actin fibre redistribution. These effects could be partially reversed by treatment with the antioxidant N-acetylcysteine. These data suggest that ATGL deficiency induces renal lipid accumulation, proteinuria and glomerular filtration barrier dysfunction and implicate increased intracellular ROS levels in inducing podocyte F-actin rearrangement, foot process fusion and apoptosis that underlie these pathological features. Enzymes: Adipose triglyceride lipase, EC22.214.171.124. © 2017 Federation of European Biochemical Societies
Yang J.,People's Care |
Chen J.,The General Hospital of the Chinese Peoples Armed Police Forces |
Liu H.,General Hospital of Chinese Peoples Armed Police Forces |
Zheng J.,The General Hospital of the Chinese Peoples Armed Police Forces
Natural Hazards | Year: 2014
Over a period of 5 years, two large earthquakes struck Sichuan Wenchuan and Lushan successively. The two main seismic zones are only 87 km apart along the same seismic belt on the Longmenshan fault. Although there was only one magnitude of difference between the two great quakes, losses from the 2013 Lushan Earthquake were much lower than that of 2008 Wenchuan Earthquake. This study compares these disasters in terms of preparation and response in order to develop effective ways to reduce casualty and economic loss in future earthquakes. By determining what was done right after the Wenchuan Earthquake, we can better understand how to reduce future losses. This study focuses on seven factors: basic information, preparedness, government response, local residents' responses, medical rescue teams' work, earthquake-induced secondary effects, and injury character. We also recommend that three major actions should be emphasized to facilitate the most effective course of disaster planning and action. First, sufficient preparedness and strict preventive measures form the foundation to minimize damage and reduce casualties. Once the disaster had occurred, a single, well-run headquarters increases efficiency in rescue efforts. Finally, local rescue strength of both professional staff and citizens is the most critical factor to lower disaster casualties. © 2014 The Author(s).