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Chen C.,General Hospital of Nanjing Military Base | Chen C.,Peking University | Luo G.-D.,Guangdong Sencee Pharmaceutical Co. | Fu H.-Z.,Peking University
Chinese Traditional and Herbal Drugs | Year: 2017

Objective: To study triterpenoids of Paliurus ramosissimus. Methods: Six triterpenoids were obtained by modern chromatography technologies, such as repeated chromatography on silica gel, ODS, Sephadex LH-20, and preparative HPLC. Their structures were elucidated on the basis of combination of IR, mass spectrometry, and NMR spectroscopy. Results: Six triterpenoids were isolated from the ethyl acetate soluble parts obtained from 95% aq. ethanol extract of the aerial parts of P. ramosissimus. They were identified as 22β,24-dihydroxy-A(1)-nor-2,20(29)-lupindien-27,28-dicarboxylic acid (1), zizyberenalic acid (2), 3-O-protocatechuoylceanothic acid (3), ceanothetric acid (4), 2-O-caffeoylalphitolic acid (5), and 3-O-caffeoylalphitolic acid (6). Conclusion: Compound 1 paliurus ramosissimus acid A is a new compound. Compounds 2-6 are isolated from P. ramosissimus for the first time. And the complete NMR data of compound 3 has been presented in this paper for the first time. © 2017, Editorial Office of Chinese Traditional and Herbal Drugs. All right reserved.


Ge Y.P.,Peking Union Medical College | Boekhout T.,Centraalbureau voor Schimmelcultures Fungal Biodiversity Center | Zhan P.,Dermatology Hospital of Jiangxi Province | Lu G.X.,Peking Union Medical College | And 4 more authors.
Medical Mycology | Year: 2012

Candida parapsilosis, which was previously considered to be a complex of three genetically distinct groups, has emerged as a significant agent of nosocomial infections. Recently, this complex was separated into three species: C. parapsilosis sensu stricto, C. orthopsilosis and C. metapsilosis. In China, data pertaining to these fungi are limited. In this study, we examined 57 isolates of members of the C. parapsilosis complex collected from four cities in East China, i.e., Nanjing (n = 22), Nanchang (n = 20), Shanghai (n = 12) and Jinan (n = 3). C. parapsilosis sensu stricto represented 71.9% of all isolates, while C. metapsilosis accounted for the remaining 28.1%. C. orthopsilosis could not be identified. A significantly high prevalence of C. metapsilosis was observed in strains recovered from Nanchang, 60% (12/20) of the isolates were C. metapsilosis. Sequence analysis of internal transcribed spacer region revealed two unevenly distributed genotypes among the C. metapsilosis strains. A PCR-restriction fragment length polymorphism assay was described for rapid identification. The strains were susceptible to fluconazole, voriconazole, amphoterincin B and micafungin. Six (15%) isolates of C. parapsilosis sensu stricto and three (18.8%) of C. metapsilosis were found to be dose-dependent susceptible to itraconazole. C. parapsilosis sensu stricto strains were less susceptible to micafungin than C. metapsilosis. © 2012 ISHAM.


PubMed | The General Hospital of Peoples Liberation Army, Shanghai JiaoTong University, Shandong University, Peking Union Medical College and 17 more.
Type: Journal Article | Journal: Antimicrobial agents and chemotherapy | Year: 2016

To evaluate the antimicrobial susceptibility of Gram-negative bacilli that caused hospital-acquired and community-acquired intra-abdominal infections (IAIs) in China between 2012 and 2013, we determined the susceptibilities to 12 antimicrobials and the extended-spectrum -lactamase (ESBL) statuses of 3,540 IAI isolates from seven geographic areas in China in a central laboratory using CLSI broth microdilution and interpretive standards. Most infections were caused by Escherichia coli (46.3%) and Klebsiella pneumoniae (19.7%). Rates of ESBL-producing E. coli (P = 0.031), K. pneumoniae (P = 0.017), and Proteus mirabilis (P = 0.004) were higher in hospital-acquired IAIs than in community-acquired IAIs. Susceptibilities of enterobacteriaceae to ertapenem, amikacin, piperacillin-tazobactam, and imipenem were 71.3% to 100%, 81.3% to 100%, 64.7% to 100%, and 83.1% to 100%, respectively, but imipenem was ineffective against P. mirabilis (<20%). Although most ESBL-positive hospital-acquired isolates were resistant to third- and fourth-generation cephalosporins, the majority were susceptible to cefoxitin (47.9% to 83.9%). Susceptibilities of ESBL-positive isolates to ampicillin-sulbactam (<10%) were low, whereas susceptibilities to ciprofloxacin (0% to 54.6%) and levofloxacin (0% to 63.6%) varied substantially. The prevalences of cephalosporin-susceptible E. coli and K. pneumoniae were higher in the northeastern and southern regions than in the central and eastern regions, reflecting the ESBL-positive rates in these areas, and were lowest in the Jiangsu-Zhejiang (Jiang-Zhe) area where the rates of carbapenem resistance were also highest. Ertapenem, amikacin, piperacillin-tazobactam, and imipenem are the most efficacious antibiotics for treating IAIs in China, especially those caused by E. coli or K. pneumoniae. Resistance to cephalosporins and carbapenems is more common in the Jiang-Zhe area than in other regions in China.


PubMed | The General Hospital of Peoples Liberation Army, Shanghai JiaoTong University, Shandong University, Peking Union Medical College and 16 more.
Type: Journal Article | Journal: Diagnostic microbiology and infectious disease | Year: 2016

Recently, the emergence of multidrug-resistant organisms such as extended-spectrum -lactamase (ESBL)-producing Enterobacteriaceae has raised considerable concern regarding the appropriate treatment of intra-abdominal infections (IAIs). In this study, we investigated the molecular characteristics of ESBL among clinical isolates of Escherichia coli and Klebsiella pneumoniae causing IAIs and their pattern of antimicrobial resistance, which can provide useful information about the epidemiology and risk factors associated with these infections.One hundred sixty-seven E.coli and 47 K. pneumoniae ESBL-producing strains causing IAIs were collected from 9 hospitals in China, during 2012 and 2013. The antimicrobial susceptibility profile of these strains was determined. Polymerase chain reaction and sequencing were performed to identify genes for -lactamase (blaTEM, blaSHV, blaOXA-1-like, and blaCTX-M). The isolates were also analyzed by pulsed-field gel electrophoresis (PFGE).In 167 ESBL-producing E. coli strains, 104 strains (62.3%) were positive for CTX-M, and 9 strains (5.39%) were positive for SHV. Among the 47 K. pneumoniae strains, 35 strains (74.5%) were positive for SHV-2a, 12 strains (25.5%) were positive for CTX-M. No TEM-type and OXA-1-like strain was detected among all the ESBL-producing strains. Regarding the CTX-M-positive E. coli and K. pneumoniae strains, CTX-M-15 was the most common genotype in E. coli and K. pneumoniae strains, accounting for 28.7% and 17.0%, respectively, followed by CTX-M-55 accounting for 16.2% and 2.13%, respectively; the remaining genotypes included CTX-M-123 and CTX-M-82. PFGE showed that E.coli and K. pneumoniae ESBL-producing strains causing IAIs were diverse and that emerging resistance may not be due to the dissemination of national clones.The present study revealed that in ESBL-producing strains causing IAIs in China, the most common genotype for E.coli was CTX-M-15 and for K. pneumoniae was SHV-2a. However, there was a wide diversity of strains causing IAIs among the ESBL-producing E. coli and K. pneumoniae.


Guo N.,Liaoning Medical University | Xue W.,General Hospital of Shenyang Military Command | Tang D.,Navy Hospital 411 | Ding J.,General Hospital of Wuhan Military | Zhao B.,General Hospital of Nanjing Military Base
American Journal of Infection Control | Year: 2016

The purpose of this study was to determine the risk factors and outcomes of bloodstream infections caused by multidrug-resistant (MDR) Acinetobacter baumannii complex in a hospital of Northern China. Risk factors associated with MDR A baumannii complex included older age, pneumonia, using drainage catheters, and intensive care unit stay. Multivariate analysis showed that multidrug resistance and mechanical ventilation were identified as independent risk factors for 30-day mortality in patients with A baumannii complex bacteremia. © 2016 Association for Professionals in Infection Control and Epidemiology, Inc.


PubMed | General Hospital of Nanjing Military Base, General Hospital of Wuhan Military, Liaoning Medical University, Navy Hospital 411 and General Hospital of Shenyang Military Command
Type: Journal Article | Journal: American journal of infection control | Year: 2016

The purpose of this study was to determine the risk factors and outcomes of bloodstream infections caused by multidrug-resistant (MDR) Acinetobacter baumannii complex in a hospital of Northern China. Risk factors associated with MDR A baumannii complex included older age, pneumonia, using drainage catheters, and intensive care unit stay. Multivariate analysis showed that multidrug resistance and mechanical ventilation were identified as independent risk factors for 30-day mortality in patients with A baumannii complex bacteremia.


Zhou Y.,Chongqing Medical University | Wang Z.,Chongqing Medical University | Chen Y.,CAS Shanghai Institute of Ceramics | Shen H.,Chongqing Medical University | And 10 more authors.
Advanced Materials | Year: 2013

A special "small to big" temperature-responsive phase-transformation strategy based on the "acoustic droplet vaporization (ADV)" mechanism is developed for efficient targeted ultrasonography and synergistic high intensity focused ultrasound (HIFU) cancer surgery by engineering targeted nanoemulsions, which is systematically evaluated and successfully demonstrated in vitro, ex vivo, and in vivo. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Niu C.,Chongqing Medical University | Wang Z.,Chongqing Medical University | Lu G.,General Hospital of Nanjing Military Base | Krupka T.M.,Stanford University | And 6 more authors.
Biomaterials | Year: 2013

Current strategies for tumor-induced sentinel lymph node detection and metastasis therapy have limitations. In this work, we co-encapsulated iron oxide nanoparticles and chemotherapeutic drug into poly(lactic-co-glycolic acid) (PLGA) microbubbles to form multifunctional polymer microbubbles (MPMBs) for both tumor lymph node imaging and therapy. Fe3O4 nanoparticles and doxorubicin (DOX) co-encapsulated PLGA microbubbles were prepared and filled with perfluorocarbon gas. Enhancement of ultrasound (US)/magnetic resonance (MR) imaging and US triggered drug delivery were evaluated both in vitro and in vivo. The MPMBs exhibited characters like narrow size distribution and smooth surface with a mean diameter of 868.0 ± 68.73 nm. In addition, varying the concentration of Fe3O4 nanoparticles in the bubbles did not significantly influence the DOX encapsulation efficiency or drug loading efficiency. Our in vitro results demonstrated that these MPMBs could enhance both US and MR imaging which was further validated in vivo showing that these MPMBs enhanced tumor lymph nodes signals. The anti-tumor effect of MPMBs mediated chemotherapy was assessed in vivo using end markers like tumor proliferation index, micro blood vessel density and micro lymphatic vessel density, which were shown consistently the lowest after the MPMBs plus sonication treatment compared to controls. In line with these findings, the tumor cell apoptotic index was found the largest after the MPMBs plus sonication treatment. In conclusion, we have successfully developed a doxorubicin loaded superparamagnetic PLGA-Iron Oxide multifunctional theranostic agent for dual-mode US/MR Imaging of lymph node, and for low frequency US triggered therapy of metastasis in lymph nodes, which might provide a strategy for the imaging and chemotherapy of primary tumor and their metastases. © 2012 Elsevier Ltd.


Liu S.-J.,Nanjing University of Traditional Chinese Medicine | Liu Z.-X.,Nanjing University of Traditional Chinese Medicine | Ju W.-Z.,Nanjing University of Traditional Chinese Medicine | Chen Z.-Y.,Nanjing University of Traditional Chinese Medicine | And 2 more authors.
Chromatographia | Year: 2010

A rapid and sensitive LC-MS-MS method was developed and validated for the determination of asiaticoside in rat plasma. Asiaticoside was extracted by protein precipitation with acetonitrile, and separated on a C18 column. The total analytical time was relatively short (4 min), and the limit of quantification was 38 ng mL-1 using 100 μL of rat plasma. Asiaticoside and the internal standard (felodipine) were monitored in the multi-reaction-monitoring mode as follows: m/z 957.4 → 469.3 and m/z 382.2 → 145.1, respectively. Calibration was linear over a concentration range from 38 to 7,600 ng mL-1, and the correlation coefficient was greater than 0.998. The recoveries of asiaticoside from plasma were better than 85%, and RSDs of inter-day and intra-day assays were below 10.1%. The method is sensitive and specific, and suitable for pharmacokinetic studies of asiaticoside in rats. © 2010 Vieweg+Teubner Verlag | Springer Fachmedien Wiesbaden GmbH.


PubMed | General Hospital of Nanjing Military Base and Nanjing University of Traditional Chinese Medicine
Type: Evaluation Studies | Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica | Year: 2014

To establish a LC-MS/MS method to determine caffeic acid, chlorogenic acid in rat plasma and study their pharmacokinetics in rats. Six Sprague-Dawley rats were intravenously injected with 4 mL x kg(-1) of Dengzhanxixin injection, respectively. Their drug plasma concentration was determined by LC-MS/MS, with tinidazole as an internal standard. The pharmacokinetic parameters were calculated by DAS 1.0. The linear concentration ranges of caffeic acid, and chlorogenic acid were 2-128 microg x L(-1) (r = 0.998 1) and 3-384 microg x L(-1) (r = 0.998 7), respectively. The methodological test showed conformance to the requirements. The intraday and inter-day variable coefficients were both less than 10.0%, indicating that both of legitimate precise and accuracy were in conformity with the requirements of biological sample analysis. For caffeic acid, the pharmacokinetic parameter t1/2beta AUC0-t, and CL were (130.91 +/- 38.77) min, (4.89 +/- 0.96) mg x min x L(-1) and (0.12 +/- 0.02) L x min(-1) x kg(-1), respectively. For chlorogenic acid, the pharmacokinetic parameter t1/2beta , AUC0-t, and CL were (49.38 +/- 8.85) min, (9.54 +/- 0.95) mg x min x L(-1) and (0.09 +/- 0.003) L x min(-1) x kg(-1), respectively. The LC-MS/MS analysis method established in this study was proved to be so accurate and sensitive that it can be applied to the pharmacokinetic study of caffeic acid and chlorogenic acid.

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