General Hospital of Liaohe Oil Field

Panjin, China

General Hospital of Liaohe Oil Field

Panjin, China
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Fan H.-B.,General Hospital of Liaohe Oil Field | Chen D.,General Hospital of Liaohe Oil Field | Ou L.-H.,General Hospital of Liaohe Oil Field | Song J.-L.,General Hospital of Liaohe Oil Field | Song G.,Urologic
Chinese Journal of Tissue Engineering Research | Year: 2013

Background: Few studies are reported at home and abroad regarding detection of leukemia stem cell concentration in childhood acute myeloid leukemia and the correlation between leukemia stem cell level in childhood acute myeloid leukemia after remission and minimal residual disease level. Objective: To determine leukemia stem cell level and leukemia stem cell-IPIC level in childhood acute myeloid leukemia both at initial diagnosis and at remission and correlate them to minimal residual disease level in childhood acute myeloid leukemia. Methods: A total of 113 samples from patients with childhood acute myeloid leukemia were collected. All heparinized bone marrow mononuclear cells were separated by cell density gradient centrifugation on ficoll-hypaque solution. After washes with PBS containing 0.1% fetal calf serum, mononuclear cell suspension was prepared. Mononuclear cells were stained with fluochrome labeled monoclonal antibodies. Leukemia stem cell level was determined and leukemia-related immunophenotypes were acquired. Immunophenotype determination and flow cytometry analysis were performed. Results and Conclusion: Leukemia stem cell level in childhood acute myeloid leukemia group at initial diagnosis was significantly higher than that in acute lymphocytic leukaemia and non-malignancy control groups (both P < 0.017). The leukemia stem cell-IPIC level in childhood acute lymphocytic leukemia at initial diagnosis was significantly higher than that in the non-malignancy control group (P < 0.017). There was a significant negative correlation between leukemia stem cell level and minimal residual disease level in childhood acute myeloid leukemia. The results indicate that (1) the phenotypically same leukemia stem cell populations were also found in the bone marrow of patients with acute lymphocytic leukaemia at initial diagnosis, but the levels were not significantly different when complete remission was achieved. These phenotypically same populations were hardly found in the non-malignancy control group. (2) There was a significant negative correlation between leukemia stem cell level in childhood acute myeloid leukemia and minimal residual disease level in childhood acute myeloid leukemia patients after remission.


Zhang Y.,First Hospital of Liaoning Medical College | Liang W.,Morphological Test Center | Peng D.,General Hospital of Liaohe Oil Field | Li C.,First Hospital of Liaoning Medical College | Gao Z.,First Hospital of Liaoning Medical College
Chinese Journal of Cancer Biotherapy | Year: 2013

Objective: To explore the clinical significance of the expressions of XIAP(X-linked inhibitor of apoptosis protein) and caspase-3 in colorectal adenocarcinoma and adenoma. Methods: Sixty-seven cases with colorectal adenocar-cinoma, 30 cases of colorectal adenoma cases selected from the Department of Pathology, First Affiliated Hospital of Liaoning Medical College from 2010 to 2012 with surgical resection, and 30 cases of corresponding adjacent mucosa (the distance from the edge of the cancerous tissue 5 cm) were used as a control. Immunohistochemistry was used to detect the expressions of XIAP and caspase-3 proteins in colorectal adenocarcinoma and adenoma tissues; Western blotting was used to detect the expression of XIAP in the colorectal adenocarcinoma and adenoma tissues; The relationship between the expression of XIAP and the clinical pathology parameters of colorectal adenocarcinoma was analyzed. Results: The positive rate of XIAP expression in the colorectal adenoma group (71. 6%) was higher than that in colorectal adenocarcinoma (46. 7%), and its expression rate was increasing with the decrease of the tissue differentiation degree (x2 = 16.132, P < 0. 05); the positive rate of caspase-3 expression in the colorectal adenocarcinoma tissues (18. 0%) was lower than that in the colorectal adenoma group (43.3%), and its expression rate was decreased with the decrease of the pathological differentiation degree (P <0. 05). The expression of XIAP protein was in a negative correlation with that of caspase-3 (r = 0. 396, P < 0. 05). Conclusion: The XIAP protein might play a significant role in promoting the progress from colorectal adenoma to colorectal adenocarcinoma bv inhibiting caspase-3.


Song Y.-q.,General Hospital of Liaohe Oil Field | Zhang Y.-m.,General Hospital of Liaohe Oil Field | Wang N.-n.,General Hospital of Liaohe Oil Field | Xiu J.,General Hospital of Liaohe Oil Field | Zhang R.-p.,General Hospital of Liaohe Oil Field
Chinese Journal of Tissue Engineering Research | Year: 2012

BACKGROUND: Intramyocardial transplantation of adipose-derived stem cells using biological technique for repair of injured myocardial tissue is a gradually increasing research field of cardiovascular disease. There has been no report describing cardiac-specific gene expression in cardiomyocytes induce-differentiated by rabbit adipose-derived stem cells. OBJECTIVE: To investigate cardiac-specific gene expression in cardiomyocytes differentiated by rabbit adipose-derived stem cells under 5-azacytidine induction. METHODS: Passage 2 adipose-derived stem cells were treated with 2.5, 5, 10, 20, 40, 80 μmo1/L 5- azacytidine for 24 hours and cultured with complete medium without 5-azacytidine. Four weeks later, cellular growth and morphological changes were observed. At the first, second, third and fourth weeks, the mRNA expression of atrial natriuretic peptide, brain natriuretic peptide and α-skeletal actin in adipose-derived stem cells was detected by RT-PCT. RESULTS AND CONCLUSION: After 5-azacytidine induction for 1 week, the majority of cells were arranged in parallel, with increased soma, and the proportion of shuttle-shaped cells was decreased, in particular in the 40, 80 μmol/L 5- azacytidine groups. At the fourth week, cell refraction was attenuated, cell activity was weakened, and more cells died in the 40, 80 μmol/L 5-azacytidine groups. The mRNA expression of atrial natriuretic peptide, brain natriuretic peptide and α-skeletal actin in adipose-derived stem cells increased gradually. The mRNA expression of atrial natriuretic peptide, brain natriuretic peptide and α-skeletal actin in the 10 μmol/L 5-azacytidine group was significantly higher than that in the other groups (P < 0.05). 5-azacytidine induced rabbit adipose-derived stem cells show cardiac-specific gene expression.


Xu L.,General Hospital of Liaohe Oil Field | Wei H.,General Hospital of Liaohe Oil Field
Journal of Cancer Research and Therapeutics | Year: 2015

Background: The aim of our study was to assess the association of CYP1A1 2454A > G polymorphism and colorectal cancer (CRC) risk. Materials and Methods: Electronic databases, including PubMed, MEDLINE Springer, Elsevier Science Direct, Cochrane Library, and Google Scholar were searched for relevant trials until December 2013. Pooled odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated to assess the strength of the association. Subgroup analyses were conducted based on geographical region and size of the study samples. Results: Thirteen eligible studies were included in this meta-analysis with 3490 cases and 4076 controls. There were significant associations under the overall ORs for G-allele comparison (G vs. A, pooled OR 1.29, 95% CI 1.03-1.61, P = 0.03), GG versus AA comparison (pooled OR 1.50, 95% CI 1.17-1.91, P < 0.01), recessive model (GG vs. GA + AA, pooled OR 1.52, 95% CI 1.20-1.94, P < 0.01) between CYP1A1 2454A > G polymorphism and CRC risk. Subgroup analyses stratified by geographical region demonstrated an association in Asia and Europe, but not in America. Conclusion : This meta-analysis suggests that CYP1A1 2454A > G polymorphism might be associated with susceptibility of CRC and the allele G might increase the CRC risk in Asia and Europe. © 2015 Journal of Cancer Research and Therapeutics | Published by Wolters Kluwer - Medknow.


Zhao J.,Shandong University | Zhao J.,General Hospital of Liaohe Oil Field | Li Z.,The General Hospital of PLA | Wang L.,Shandong University | And 5 more authors.
Transplant Immunology | Year: 2015

Objective: The study aimed to investigate whether Foxp3-expressing sensitized Teff cells could inhibit allograft rejection in corneal allograft transplantation mouse model. Methods: Foxp3-expressing sensitized Teff cells were constructed by transfection of retroviral expression plasmid expressing Foxp3 into the sensi-Teff cells from a Balb/c mouse immunized by C57BL/6(H2b) mouse splenocytes. Balb/c mice were randomly divided into 5 groups: Four groups received tail vein injection of Foxp3-expressing sensitized Teff cells, or Foxp3-expressing Teff cells, or Treg cells or no intervention 1day prior to corneal allograft transplantation. C57BL/6(H2b) was the donor mouse. The last group received corneal autograft transplantation. Corneal allograft survival time and percentage of CD4+ T cells were detected. ELISPOT and Footpad swelling test were used to measure IL-2 and IFN-γ, and delayed-type hypersensitivity (DTH) response, respectively. Results: Mice that had received an injection of Foxp3-expressing sensitized T cells prior to an allograft corneal transplantation, showed significantly longer survival time of corneal allograft, decreased percentage of CD4+ T cells, IL-2 and IFN-γ, and alleviated footpad swelling than the mice that had received either Foxp3-Teff or Treg cells. Conclusion: Foxp3-sensi-Teff cell treatment that prolongs corneal allograft survival in the mouse model, might partly through suppressing CD4+ T cells, IL-2 and IFN-γ. © 2015 Elsevier B.V.


Yang J.,Liaoning Medical University | Zhu X.-B.,General Hospital of Liaohe Oil Field | He L.-X.,Fushun Second Hospital | Gu Z.-W.,Liaoning Medical University | And 2 more authors.
Oncology Letters | Year: 2015

The aim of the present study was to investigate the association between O6-methylguanine-DNA methyltrans­ferase (MGMT) gene expression levels, and DNA methylation status and histone modifications in laryngeal squamous cell carcinoma (LSCC). Chromatin immunoprecipitation, meth­ylation-specific polymerase chain reaction (PCR), and reverse transcription-quantitative PCR were performed to analyze histone modifications, DNA methylation status and mRNA expression levels in the promoter region of the MGMT gene in laryngeal carcinoma HEp-2 cells, as well as in 50 paired healthy and LSCC tissue samples. The present study demonstrated that treatment of HEp-2 cells with 5-aza-2'-deoxycytidine (Aza), a DNA methyltransferase inhibitor, significantly upregulated MGMT mRNA expression levels, reduced MGMT DNA methylation, reduced MGMT histone H3 lysine 9 (H3K9) di-methylation, and increased MGMT histone H3 lysine 4 di-methylation without a signifi­cant change in H3K9 acetylation. Trichostatin A (TSA), a histone deacetylase inhibitor, marginally upregulated MGMT mRNA expression levels without affecting the DNA methyla­tion status, or H3K9 or H3K4 di-methylation, however, TSA treatment caused a significant increase in H3K9 acetylation. Furthermore, Aza and TSA combination treatment produced a synergistic effect. In the LSCC samples, the rate of DNA methylation in the MGMT gene was 54%, compared with 24% in the healthy control group (P<0.05). Therefore, data from the present study indicates that MGMT may serve as a novel therapeutic target in the treatment of LSCC. © 2015, Spandidos Publications. All rights reserved.


Chen G.-L.,Shandong University | Zhang J.-J.,Third Peoples Hospital of Jinan | Zhao J.,General Hospital of Liaohe Oil Field | Wang D.-J.,General Hospital of Peoples Liberation Army | Zhang H.,Shandong University
International Journal of Ophthalmology | Year: 2013

AIM: To investigate the characteristics and criterion of graft rejection in mice model. METHODS: C57BL/6 or BALB/c mice corneal grafts were grafted onto BALB/c hosts. Each group was divided into two subgroups according to the corneal opacity scores 12d after transplantation. The characteristics of opacity and neovascularization were observed. Mice of the 12th, 50th day after transplantation, the grafts biopsy of mice in allogeneic group 1, which opacity score exceed 3, were prepared for histological observation and those restore transparent were endothelial stained. RESULTS: There was no difference of corneal opacity score on the 7th and 12th day after operation; the histological results had no disparity between syngeneic group and allogeneic group. On the 12th day after surgery, the turbidity curve was apparent in grafts with opacity score <2. Mononuclear cells were shown in grafts with opacity score reached 3 in allogeneic group 1. Different rejection performance was observed in tissue sections on the 50th day after surgery. CONCLUSION: Grafts, opacity score exceeds 3 from the 7th to the 12th day after operation could not be judged as a rejection. We should pay more attention to the variation of grafts opacity since 12d after corneal transplantation. Copyright International Journal of Ophthalmology Press.


PubMed | General Hospital of Liaohe Oil Field, University of California at Los Angeles, Shandong University, Zibo Kangming Ophthalmology Hospital and Dalian Medical University
Type: Journal Article | Journal: International journal of ophthalmology | Year: 2016

To compare the outcomes of vision using two different intraocular lens (IOL) replacement techniques, iris-fixated foldable intraocular lens (IF-IOL) and scleral-fixated foldable intraocular lens (SF-IOL) in patients with insufficient capsular support.Total 63 eyes (62 patients) with insufficient posterior capsule support underwent replacement of IF-IOL or SF-IOL between January 2008 and August 2011. Outcome measures included changes in visual acuity, slit lamp examination, refractive indices and corneal curvatures.The mean improvement of uncorrected visual acuity (UCVA) was greater in IF-IOL group compared to the SF-IOL group (0.43 D0.19 D IF-IOL implantation can give a significant improvement in vision with fewer complications than SF-IOL in patients with insufficient capsular support.


PubMed | The General Hospital of PLA, General Hospital of Liaohe Oil Field and Shandong University
Type: Journal Article | Journal: Transplant immunology | Year: 2015

The study aimed to investigate whether Foxp3-expressing sensitized Teff cells could inhibit allograft rejection in corneal allograft transplantation mouse model.Foxp3-expressing sensitized Teff cells were constructed by transfection of retroviral expression plasmid expressing Foxp3 into the sensi-Teff cells from a Balb/c mouse immunized by C57BL/6(H2b) mouse splenocytes. Balb/c mice were randomly divided into 5 groups: Four groups received tail vein injection of Foxp3-expressing sensitized Teff cells, or Foxp3-expressing Teff cells, or Treg cells or no intervention 1 day prior to corneal allograft transplantation. C57BL/6(H2b) was the donor mouse. The last group received corneal autograft transplantation. Corneal allograft survival time and percentage of CD4(+) T cells were detected. ELISPOT and Footpad swelling test were used to measure IL-2 and IFN-, and delayed-type hypersensitivity (DTH) response, respectively.Mice that had received an injection of Foxp3-expressing sensitized T cells prior to an allograft corneal transplantation, showed significantly longer survival time of corneal allograft, decreased percentage of CD4(+) T cells, IL-2 and IFN-, and alleviated footpad swelling than the mice that had received either Foxp3-Teff or Treg cells.Foxp3-sensi-Teff cell treatment that prolongs corneal allograft survival in the mouse model, might partly through suppressing CD4(+) T cells, IL-2 and IFN-.


PubMed | General Hospital of Liaohe Oil Field
Type: Journal Article | Journal: Journal of cancer research and therapeutics | Year: 2016

The aim of our study was to assess the association of CYP1A1 2454A > G polymorphism and colorectal cancer (CRC) risk.Electronic databases, including PubMed, MEDLINE Springer, Elsevier Science Direct, Cochrane Library, and Google Scholar were searched for relevant trials until December 2013. Pooled odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated to assess the strength of the association. Subgroup analyses were conducted based on geographical region and size of the study samples.Thirteen eligible studies were included in this meta-analysis with 3490 cases and 4076 controls. There were significant associations under the overall ORs for G-allele comparison (G vs. A, pooled OR 1.29, 95% CI 1.03-1.61, P = 0.03), GG versus AA comparison (pooled OR 1.50, 95% CI 1.17-1.91, P < 0.01), recessive model (GG vs. GA + AA, pooled OR 1.52, 95% CI 1.20-1.94, P < 0.01) between CYP1A1 2454A > G polymorphism and CRC risk. Subgroup analyses stratified by geographical region demonstrated an association in Asia and Europe, but not in America.This meta-analysis suggests that CYP1A1 2454A > G polymorphism might be associated with susceptibility of CRC and the allele G might increase the CRC risk in Asia and Europe.

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