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Zhang Y.,First Hospital of Liaoning Medical College | Liang W.,Morphological Test Center | Peng D.,General Hospital of Liaohe Oil Field | Li C.,First Hospital of Liaoning Medical College | Gao Z.,First Hospital of Liaoning Medical College
Chinese Journal of Cancer Biotherapy | Year: 2013

Objective: To explore the clinical significance of the expressions of XIAP(X-linked inhibitor of apoptosis protein) and caspase-3 in colorectal adenocarcinoma and adenoma. Methods: Sixty-seven cases with colorectal adenocar-cinoma, 30 cases of colorectal adenoma cases selected from the Department of Pathology, First Affiliated Hospital of Liaoning Medical College from 2010 to 2012 with surgical resection, and 30 cases of corresponding adjacent mucosa (the distance from the edge of the cancerous tissue 5 cm) were used as a control. Immunohistochemistry was used to detect the expressions of XIAP and caspase-3 proteins in colorectal adenocarcinoma and adenoma tissues; Western blotting was used to detect the expression of XIAP in the colorectal adenocarcinoma and adenoma tissues; The relationship between the expression of XIAP and the clinical pathology parameters of colorectal adenocarcinoma was analyzed. Results: The positive rate of XIAP expression in the colorectal adenoma group (71. 6%) was higher than that in colorectal adenocarcinoma (46. 7%), and its expression rate was increasing with the decrease of the tissue differentiation degree (x2 = 16.132, P < 0. 05); the positive rate of caspase-3 expression in the colorectal adenocarcinoma tissues (18. 0%) was lower than that in the colorectal adenoma group (43.3%), and its expression rate was decreased with the decrease of the pathological differentiation degree (P <0. 05). The expression of XIAP protein was in a negative correlation with that of caspase-3 (r = 0. 396, P < 0. 05). Conclusion: The XIAP protein might play a significant role in promoting the progress from colorectal adenoma to colorectal adenocarcinoma bv inhibiting caspase-3. Source


Zhao J.,Shandong University | Li Z.,Chinese PLA General Hospital | Wang L.,Shandong University | Liu J.,Shandong University | And 4 more authors.
Transplant Immunology | Year: 2015

Objective: The study aimed to investigate whether Foxp3-expressing sensitized Teff cells could inhibit allograft rejection in corneal allograft transplantation mouse model. Methods: Foxp3-expressing sensitized Teff cells were constructed by transfection of retroviral expression plasmid expressing Foxp3 into the sensi-Teff cells from a Balb/c mouse immunized by C57BL/6(H2b) mouse splenocytes. Balb/c mice were randomly divided into 5 groups: Four groups received tail vein injection of Foxp3-expressing sensitized Teff cells, or Foxp3-expressing Teff cells, or Treg cells or no intervention 1day prior to corneal allograft transplantation. C57BL/6(H2b) was the donor mouse. The last group received corneal autograft transplantation. Corneal allograft survival time and percentage of CD4+ T cells were detected. ELISPOT and Footpad swelling test were used to measure IL-2 and IFN-γ, and delayed-type hypersensitivity (DTH) response, respectively. Results: Mice that had received an injection of Foxp3-expressing sensitized T cells prior to an allograft corneal transplantation, showed significantly longer survival time of corneal allograft, decreased percentage of CD4+ T cells, IL-2 and IFN-γ, and alleviated footpad swelling than the mice that had received either Foxp3-Teff or Treg cells. Conclusion: Foxp3-sensi-Teff cell treatment that prolongs corneal allograft survival in the mouse model, might partly through suppressing CD4+ T cells, IL-2 and IFN-γ. © 2015 Elsevier B.V. Source


Yang J.,Liaoning Medical University | Zhu X.-B.,General Hospital of Liaohe Oil Field | He L.-X.,Fushun Second Hospital | Gu Z.-W.,Liaoning Medical University | And 2 more authors.
Oncology Letters | Year: 2015

The aim of the present study was to investigate the association between O6-methylguanine-DNA methyltrans­ferase (MGMT) gene expression levels, and DNA methylation status and histone modifications in laryngeal squamous cell carcinoma (LSCC). Chromatin immunoprecipitation, meth­ylation-specific polymerase chain reaction (PCR), and reverse transcription-quantitative PCR were performed to analyze histone modifications, DNA methylation status and mRNA expression levels in the promoter region of the MGMT gene in laryngeal carcinoma HEp-2 cells, as well as in 50 paired healthy and LSCC tissue samples. The present study demonstrated that treatment of HEp-2 cells with 5-aza-2'-deoxycytidine (Aza), a DNA methyltransferase inhibitor, significantly upregulated MGMT mRNA expression levels, reduced MGMT DNA methylation, reduced MGMT histone H3 lysine 9 (H3K9) di-methylation, and increased MGMT histone H3 lysine 4 di-methylation without a signifi­cant change in H3K9 acetylation. Trichostatin A (TSA), a histone deacetylase inhibitor, marginally upregulated MGMT mRNA expression levels without affecting the DNA methyla­tion status, or H3K9 or H3K4 di-methylation, however, TSA treatment caused a significant increase in H3K9 acetylation. Furthermore, Aza and TSA combination treatment produced a synergistic effect. In the LSCC samples, the rate of DNA methylation in the MGMT gene was 54%, compared with 24% in the healthy control group (P<0.05). Therefore, data from the present study indicates that MGMT may serve as a novel therapeutic target in the treatment of LSCC. © 2015, Spandidos Publications. All rights reserved. Source


Chen G.-L.,Shandong University | Zhang J.-J.,Third Peoples Hospital of Jinan | Zhao J.,General Hospital of Liaohe Oil Field | Wang D.-J.,General Hospital of Peoples Liberation Army | Zhang H.,Shandong University
International Journal of Ophthalmology | Year: 2013

AIM: To investigate the characteristics and criterion of graft rejection in mice model. METHODS: C57BL/6 or BALB/c mice corneal grafts were grafted onto BALB/c hosts. Each group was divided into two subgroups according to the corneal opacity scores 12d after transplantation. The characteristics of opacity and neovascularization were observed. Mice of the 12th, 50th day after transplantation, the grafts biopsy of mice in allogeneic group 1, which opacity score exceed 3, were prepared for histological observation and those restore transparent were endothelial stained. RESULTS: There was no difference of corneal opacity score on the 7th and 12th day after operation; the histological results had no disparity between syngeneic group and allogeneic group. On the 12th day after surgery, the turbidity curve was apparent in grafts with opacity score <2. Mononuclear cells were shown in grafts with opacity score reached 3 in allogeneic group 1. Different rejection performance was observed in tissue sections on the 50th day after surgery. CONCLUSION: Grafts, opacity score exceeds 3 from the 7th to the 12th day after operation could not be judged as a rejection. We should pay more attention to the variation of grafts opacity since 12d after corneal transplantation. Copyright International Journal of Ophthalmology Press. Source


Fan H.-B.,General Hospital of Liaohe Oil Field | Chen D.,General Hospital of Liaohe Oil Field | Ou L.-H.,General Hospital of Liaohe Oil Field | Song J.-L.,General Hospital of Liaohe Oil Field | Song G.,Urologic
Chinese Journal of Tissue Engineering Research | Year: 2013

Background: Few studies are reported at home and abroad regarding detection of leukemia stem cell concentration in childhood acute myeloid leukemia and the correlation between leukemia stem cell level in childhood acute myeloid leukemia after remission and minimal residual disease level. Objective: To determine leukemia stem cell level and leukemia stem cell-IPIC level in childhood acute myeloid leukemia both at initial diagnosis and at remission and correlate them to minimal residual disease level in childhood acute myeloid leukemia. Methods: A total of 113 samples from patients with childhood acute myeloid leukemia were collected. All heparinized bone marrow mononuclear cells were separated by cell density gradient centrifugation on ficoll-hypaque solution. After washes with PBS containing 0.1% fetal calf serum, mononuclear cell suspension was prepared. Mononuclear cells were stained with fluochrome labeled monoclonal antibodies. Leukemia stem cell level was determined and leukemia-related immunophenotypes were acquired. Immunophenotype determination and flow cytometry analysis were performed. Results and Conclusion: Leukemia stem cell level in childhood acute myeloid leukemia group at initial diagnosis was significantly higher than that in acute lymphocytic leukaemia and non-malignancy control groups (both P < 0.017). The leukemia stem cell-IPIC level in childhood acute lymphocytic leukemia at initial diagnosis was significantly higher than that in the non-malignancy control group (P < 0.017). There was a significant negative correlation between leukemia stem cell level and minimal residual disease level in childhood acute myeloid leukemia. The results indicate that (1) the phenotypically same leukemia stem cell populations were also found in the bone marrow of patients with acute lymphocytic leukaemia at initial diagnosis, but the levels were not significantly different when complete remission was achieved. These phenotypically same populations were hardly found in the non-malignancy control group. (2) There was a significant negative correlation between leukemia stem cell level in childhood acute myeloid leukemia and minimal residual disease level in childhood acute myeloid leukemia patients after remission. Source

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