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Kardítsa, Greece

Flouris A.D.,University of Thessaly | Flouris A.D.,Center for Research and Technology Hellas | Dinas P.C.,University of Thessaly | Tsitoglou K.,University of Thessaly | And 4 more authors.
Physiological Measurement | Year: 2015

We introduce a non-invasive and accurate method to assess tibialis anterior muscle temperature (Tm) during rest, cycling exercise, and post-exercise recovery using the insulation disk (INDISK) technique. Twenty-six healthy males (23.6 ±6.2 years; 24.1 ±3.1 body mass index) were randomly allocated into the 'model' (n = 16) and the 'validation' (n = 10) groups. Participants underwent 20 min supine rest, 20 min cycling exercise at 60% of age-predicted maximum heart rate, and 20 min supine post-exercise recovery. In the model group, Tm (34.55 ±1.02 °C) was greater than INDISK temperature (Tid; 32.44 ±1.23 °C; p < 0.001) and skin surface temperature (Tsk; 29.84 ±1.47 °C; p < 0.001) throughout the experimental protocol. The strongest prediction model (R2 = 0.646) incorporated Tid and the difference between the current Tid temperature and that recorded four minutes before. No mean difference (p > 0.05) and a strong correlation (r = 0.804; p < 0.001) were observed between Tm and predicted Tm (predTm) in the model group. Cross-validation analyses in the validation group demonstrated no mean difference (p > 0.05), a strong correlation (r = 0.644; p < 0.001), narrow 95% limits of agreement (-0.06 ±1.51), and low percent coefficient of variation (2.24%) between Tm (34.39 ±1.00 °C) and predTm (34.45 ±0.73 °C). We conclude that the novel technique accurately predicts Tm during rest, cycling exercise, and post-exercise recovery, providing a valid and cost-efficient alternative when direct Tm measurement is not feasible. © 2015 Institute of Physics and Engineering in Medicine.

Samaras A.,General Hospital of Giannitsa | Tsarouhas K.,General Hospital of Karditsa | Paschalidis E.,General Hospital of Giannitsa | Giamouzis G.,University of Thessaly | And 5 more authors.
Food and Chemical Toxicology | Year: 2014

It is well established that exercise induces excessive production of reactive species leading to oxidative stress, which has been implicated in oxidative damage of macromolecules, immune dysfunction, muscle damage and fatigue. The present study examined the effect of supplementation of ultra-marathon runners for a two-months-period with a special whey protein bar containing carbohydrates and protein in a specific ratio (1:1) (N= 16), prepared using as starting material the by-products of cheese manufacturing, and supplementation with commercially available tomato juice (N= 15). Thiobarbituric-acid reactive substances and protein carbonyls were significantly decreased in both supplementation groups, while a pronounced increased in reduced glutathione was observed in the protein bar group. Total anti-oxidant activity remained unchanged in both groups. Flow-mediated dilatation, used as an estimate of endothelial function, was increased in both groups, with a significant rise observed only in the tomato juice administration group. In conclusion, supplementation of ultra marathon runners for a two-months-period with a special protein bar and tomato juice significantly improved the oxidative status of the subjects, while tomato juice also improved vascular endothelial function in these athletes. © 2014 .

Germanakis I.,University of Crete | Tsarouhas K.,General Hospital of Karditsa | Fragkiadaki P.,University of Crete | Tsitsimpikou C.,General Chemical State Laboratory of Greece | And 5 more authors.
Food and Chemical Toxicology | Year: 2013

Highlights:The present study focuses on the short term effects of repeated low level administration of turinabol and methanabol on cardiac function in young rabbits (4. months-old). The experimental scheme consisted of two oral administration periods, lasting 1. month each, interrupted by 1-month wash-out period. Serial echocardiographic evaluation at the end of all three experimental periods was performed in all animals. Oxidative stress markers have also been monitored at the end of each administration period. Treated animals originally showed significantly increased myocardial mass and systolic cardiac output, which normalized at the end of the wash out period. Re-administration led to increased cardiac output, at the cost though of a progressive myocardial mass reduction. A dose-dependent trend towards impaired longitudinal systolic, diastolic and global myocardial function was also observed. The adverse effects were more pronounced in the methanabol group. For both anabolic steroids studied, the low dose had no significant effects on oxidative stress markers monitored, while the high dose created a hostile oxidative environment. In conclusion, anabolic administration has been found to create a possible deleterious long term effect on the growth of the immature heart and should be strongly discouraged especially in young human subjects. © 2013 Elsevier Ltd.

Liatsos G.D.,General Hospital of Athens G. Gennimatas | Thanellas S.,General Hospital of Karditsa | Pirounaki M.,Hippokration General Hospital of Athens | Ketikoglou I.,Hippokration General Hospital of Athens | Moulakakis A.,Hippokration General Hospital of Athens
Scandinavian Journal of Gastroenterology | Year: 2012

Backround. Spontaneous bacterial peritonitis (SBP) is a serious complication in cirrhotic patients. Gram () (E. coli, Klebsiella pneumoniae), and Gram () (Streptococci, Staphylococci) bacteria are most frequently cultured from patients'ascites. Listeria monocytogenes (Lm) is scarcely reported as a causative agent. Objective. Our objective is to describe Lm peritonitis as a clinical entity, including its presentation, clinical features, treatment, and the potential factors that might affect survival outcome. Material and methods. Data sources: MEDLINE, Scholar.Google, Scopus databases, including English, Spanish, French, and German language papers published between 1966 and June 2011, and reference lists. Data extraction: investigators abstracted details about medical history, disease presentation, laboratory data, treatment and outcome. Data synthesis: One-hundred and twenty-eight cases with known survival outcome eighty-six cirrhotics, seventeen individuals undergoing continuous ambulatory peritoneal dialysis and another twenty-five with other or no underline condition were reviewed. An additional number of twenty-five cases with unknown outcome were searched in Listeria studies published from 1990 to 2009 and were only used for calculating worldwide distribution. Conclusion. Cirrhotics, mostly alcoholics, presented with fever and abdominal pain. Those who succumbed had significantly higher peripheral WBC count (15622 vs. 8155 cells/mm3, p 0.01) and (%) polymorphonuclear cells in differential count (83.3 vs. 71%, p 0.001). Higher mortality was experienced in those with comorbidities, and those who presented with encephalopathy. Lower mortality was experienced in patients on continuous ambulatory peritoneal dialysis. Ascites was neutrocytic in 86% of the samples. In the sum of the cases mortality was 27.3%, with significantly highest rates in the elderly, in patients with bacteremia, immunosuppression, hematological malignancies, and lowest rates in those who presented with abdominal pain and in diabetics (type I or II). The latter observation was surprising and could be considered a single fortuitous fact. Initial appropriate treatment was associated with significantly better outcome (p 0.002) than inappropriate; combination therapy with an aminoglycoside was superior to monotherapy (p 0.038). © Informa Healthcare.

Tsitsimpikou C.,General Chemical State Laboratory of Greece | Tsarouhas K.,General Hospital of Karditsa | Stamatopoulos P.,ORTHOBIOTIKI Prevention and Anti aging | Rentoukas E.,Amalia Fleming General Hospital of Athens | And 5 more authors.
Food and Chemical Toxicology | Year: 2013

Creatinine phosphokinase (CPK) and lactate dehydrogenase (LDH) are important biological markers of various myocardial disorders and exercise-induced muscle damage. Lycopene, on the other side, is a natural anti-oxidant with protective action against cardiovascular risk. Fifteen anaerobically trained athletes with elevated LDH and CPK baseline levels were enrolled in this study after undergoing thorough biochemical and cardiovascular evaluation with echocardiocraphy. In nine athletes tomato juice, a lycopene plain juice, was administered during and after exercise, replacing the carbohydrate supplementation beverages commonly used during training for over a 2-month period. Tomato juice administration significantly reduced LDH and CPK levels, which returned back to almost normal levels. At the same time homocysteine and C-reactive protein were also attenuated. No changes were observed in the control group, where the usual carbohydrate supplementation had been followed. © 2012 Elsevier Ltd.

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