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Zhang Y.,General Hospital of Jinan Command PLA | Mu N.-N.,General Hospital of Jinan Command PLA | Wang H.,General Hospital of Jinan Command PLA | Dong L.,General Hospital of Jinan Command PLA
Chinese Journal of Medical Imaging Technology | Year: 2011

Objective: To analyze the clinical application of color Doppler flow imaging (CDFI) and contrast-enhanced ultrasonography in diagnosis of nutcracker syndrome (NCS). Methods: Totally 80 patients clinically suspected as NCS underwent CDFI (patient group), of which 17 patients were inspected with contrast-enhanced ultrasonography. And we choosed 40 cases of healthy to be control group (P<0.01).The angle (β) between superior mesenteric artery (SMA) and abdominal aorta (AO), the interior diameter (Db, Da) of compression (b) and the proximal expansion (a) section of left renal vein (LRV), as well as the flow velocity Va and Vb were measured. Da/Db and Vb/Va were calculated. Results: (1)There were 40 NCS diagnosised with CDFI in patient group, of which 15 were diagnos ed combining with contrast-enhanced ultrasonography. The angle β with horizontal supines was (28±11)° and (19±9)° with standing supines 20 min (P<0.01). And it was (48±14)° and (37±12)° in control group (P<0.05). There was significant difference in patient group compared with control group. (2)LRV was significantly compressed in horizontal supines and standing for 20 min at patient group, Da/Db and Vb/Va were statistically different in both position (all P<0.001). The blood flow velocity at proximal expansion(a) reduced, while there was a colorful mosaic at b. The diameter and velocity of LRV were clearly showed with contrast-enhanced ultrasonography in vein phases. There was significant difference for Da/Db, Vb/Va compare with horizontal supines (all P<0.001), but not significant difference compare with those of standing for 20 min (P>0.05). It was not compressed in horizontal supines and standing for 20 min in control group. There were significant difference for Da/Db, Vb/Va in patient group compared with control group (P<0.001). Conclusion: NCS can be accurately diagnosed with CDFI combining with contrast-enhanced ultrasonography. Source


Kong F.,General Hospital of Jinan Command PLA | Zhou F.,General Hospital of Jinan Command PLA | Ge L.,General Hospital of Jinan Command PLA | Liu X.,General Hospital of Jinan Command PLA | Wang Y.,General Hospital of Jinan Command PLA
International Journal of Nanomedicine | Year: 2012

Background: Liposomes can be modified with different ligands to control their biological properties, such as longevity, targeting ability, and intracellular penetration, in a desired fashion. The aim of this study was to modify liposomes with a novel mannosylated polyethylene glycolphosphatidylethanolamine (M-PEG-PE) ligand to achieve active targeted gene delivery. Methods: Rat Kupffer cells were isolated and used as model cells for in vitro evaluation of cytotoxicity and transfection efficiency. The modified liposomes were intravenously injected into the rats, and Kupffer cells were isolated and analyzed by flow cytometry for in vivo gene delivery and expression. Results: The M-PEG-PE-modified liposome-enhanced green fluorescence protein plasmid (M-PEG-PE-Lipo-pEGFP) complexes had a particle size of 237 nm and a loading efficiency of 90%. The M-PEG-PE-Lipo-pEGFP complexes displayed remarkably higher transfection efficiency than unmodified Lipo-pEGFP, both in vitro (51%-30%) and in vivo (43%-27%). Conclusion: M-PEG-PE could function as an excellent active targeting ligand, and M-PEG-PEmodified liposomes could be promising active targeted drug delivery vectors. © 2012 Kong et al, publisher and licensee Dove Medical Press Ltd. Source

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