Zhang Y.,Peking University |
Chen H.,Peking University |
Zhao C.,Shanghai Ruijin Hospital |
Zhou B.,General Hospital Group of Daqing Oil field
Medical Journal of Wuhan University | Year: 2010
Objective: To investigate the role of breast cancer anti-estrogen resistance-3 (BCAR3) in epithelial-mesenchymal transition (EMT) induction of tamoxifen resistant of breast cancer cells, and to determine the relationship between BCAR3 induced tamoxifen resistance and invasiven metastatic behaviors. Methods: Real time PCR and Western blot were used to detect BCAR3 expression levels in epithelium and mesenchymal like breast cancer cell lines. The expression levels of E-cadherin, N-cadherin, and Twist were assessed by Western blot in MCF-7, MCF-BCAR3, and BT-549 cancer cells. Transwell migratory and invasive assays were employed to detect BCAR3 overexpression induced cell migration and invasion. Results: BCAR3 was expressed at higher levels in mesenchymal like- than in epithelial like- breast cancer cell lines. Stable over-expression of BCAR3 in MCF-7 cells leads to downregulation of E-cadherin expression and upregulation of Twist and N-cadherin expression. Coincidently in cells with relatively low migratory potential, BCAR3 overexpression resulted in enhanced migration and invasion. Conclusion: During the development of tamoxifen resistance, BCAR3 may regulate breast cell migration by promotion of EMT, thereby coordinates multiple signaling pathways.