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Yang X.-K.,General Hospital of Chengdu Military Command Area | Su J.,Peoples Hospital of Sichuan Province | Xu G.-S.,General Hospital of Chengdu Military Command Area
Tropical Journal of Pharmaceutical Research | Year: 2014

Purpose: To investigate the protective effects of various resuscitating fluids on severe hemorrhagic shocked (HS) rats by comparing the expression changes of hsp90α in cardiac muscles and survival of rats. Methods: Western-blot and immunohistochemistry methods were performed to determine hsp90α expressions in cardiac muscles of HS rats following treatment with different resuscitation fluids. In addition, the survival rates of of all the test groups were investigated. Results: The expression of hsp90α decreased following treatment with resuscitation fluids based on both Western blot and immunohistochemistry data. Ringer lactate solution (RLS) was the most effective therapeutic fluid in this regard, and this was buttressed by survival rate data (90%). Conclusion: The expression of hsp90α in rat cardiac muscle is decreased to various degrees by treatment with different resuscitating fluids, with the effect most pronounced for ringer lactate solution. Furthermore, hsp90α plays an important role in hemorrhagic rat cardiac muscle as a regulatory factor. © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved.


Li J.,General Hospital of Chengdu Military Command Area | Liu Y.-H.,General Hospital of Chengdu Military Command Area | Ou S.,General Hospital of Chengdu Military Command Area | Dai X.-M.,General Hospital of Chengdu Military Command Area | And 2 more authors.
Molecular Medicine Reports | Year: 2012

Steroid receptor coactivator-3 (SRC-3) is a transcriptional coactivator that plays an important role in the regulation of cytokine mRNA translation. In the present study, SCR-3 gene knockout mice were used to study the effects of SCR-3 on the regulation of the inflammatory response in peritoneal macrophages induced by lipopolysaccharides (LPS). Peritoneal macrophages (PMs) of SRC-3 -/- mice showed a decrease in the release of TNF-α, IL-1β and IL-6, and an increase in the release of IL-10. Furthermore, results of RT-PCR also showed that levels of TNF-α, IL-1β and IL-6 mRNA expression were significantly lower, while the level of IL-10 mRNA expression was higher in the SRC-3 -/- mice, compared to those of wild-type mice, following treatment with LPS (p<0.01). In addition, western blotting revealed that: i) the extent of reduction of the glucocorticoid receptor in PMs from SRC-3 -/- mice was significantly lower than that in wild-type mice (p<0.01); ii) the extent of increase of AP-1 in PMS from SRC-3 -/- mice was significantly lower than that in wild-type mice (p<0.01); iii) the extent of increase of NF-κB p65 in PMs from SRC-3 -/- mice was significantly higher than that in wild-type mice (p<0.01). Collectively, our studies revealed that SRC-3 may play a key role in the maintenance of innate immunity. Furthermore, absence of the SRC-3 protein may result in the partial loss of inflammation and phagocytosis barrier function, including suppression of LPS-induced transcriptional activity, release of TNF-α, IL-1β and IL-6, and obstruction of the function of phagocytes and elimination of bacteria, as well as their production.


Liu Y.-H.,General Hospital of Chengdu Military Command Area | Ye Z.-Y.,General Hospital of Chengdu Military Command Area | Liu H.-N.,General Hospital of Chengdu Military Command Area | Ou S.,General Hospital of Chengdu Military Command Area
World Journal of Gastroenterology | Year: 2011

AIM: To observe the hepatic injury induced by carbon dioxide pneumoperitoneum (CDP) in rabbits, compare the effects oflow- and high-pressure pneumoperitoneum, and to determine the degree ofhepatic injury induced by these two clinically relevant CDPpressures. METHODS: Thirty healthy male New Zealand rabbits weighing 3.0 to 3.5 kg were randomly divided into three groups (n = 10 for each group) and subjected to the following to CDPpressures: no gas control, 10 mmHg, or 15 mmHg. Histological changes in liver tissues were observed with hematoxylin and eosin staining and transmission electron microscopy. Liver function was evaluated using an automatic biochemical analyzer. Adenine nucleotide translocator (ANT) activity in liver tissue was detected with the atractyloside-inhibitor stop technique. Bax and Bcl-2 expression levels were detected by western blotting. RESULTS: Liver functions in the 10 mmHg and 15 mmHg experimental groups were significantly disturbed compared with the control group. After CDP, the levels ofalanine transaminase and aspartate transaminase were 77.3 ± 14.5 IU/L and 60.1 ± 11.4 IU/L, respectively, in the 10 mmHg experimental group and 165.1 ± 19.4 IU/L and 103.8 ± 12.3 IU/L, respectively, in the 15 mmHg experimental group, which were all higher than those ofthe control group (P< 0.05). There was no difference in pre-albumin concentration between the 10 mmHg experimental group and the control group, but the pre-albumin level ofthe 15 mmHg experimental group was significantly lower than that of the control group (P< 0.05). No significant differences were observed in the levels oftotal bilirubin or albumin among the three groups. After 30 and 60 min ofCDP, pH was reduced (P< 0.05) and PaCO 2 was elevated (P< 0.05) in the 10 mmHg group compared with controls, and these changes were more pronounced in the 15 mmHg group. Hematoxylin and eosin staining showed no significant change in liver morphology, except for mild hyperemia in the two experimental groups. Transmission electron microscopy showed mild mitochondrial swelling in hepatocytes ofthe 10 mmHg group, and this was more pronounced in the 15 mmHg group. No significant difference in ANT levels was found between the control and 10 mmHg groups. However, ANT concentration was significantly lower in the 15 mmHg group compared with the control group. The expression ofhepatic Bax was significantly increased in the two experimental groups compared with the controls, but there were no differences in Bcl-2 levels among the three groups. Twelve hours after CDPinduction, the expression ofhepatic Bax was more significant in the 15 mmHg group than in the 10 mmHg group. CONCLUSION: A CDPpressure of15 mmHg caused more substantial hepatic injury, such as increased levels ofacidosis, mitochondrial damage, and apoptosis; therefore, 10 mmHg CDPis preferable for laparoscopic operations.2011 Baishideng. All rights reserved.


Li J.,General Hospital of Chengdu Military Command Area | Niu J.,General Hospital of Chengdu Military Command Area | Ou S.,General Hospital of Chengdu Military Command Area | Ye Z.-Y.,General Hospital of Chengdu Military Command Area | And 4 more authors.
Molecular and Cellular Biochemistry | Year: 2012

Steroid receptor coactivator-3 (SRC-3) is a multifunctional protein that plays an important role in mammary gland growth, development, and tumorigenesis. In this study, SCR-3 gene knockout mice were used to study the effects of SCR-3 on the immunosuppression accompanied with systemic inflammatory response syndrome (SIRS). Bacterial clearance assay was performed by blood culture and frozen sections, and the results showed that the absence of SCR-3 protein serious damaged the innate immune system and the body's ability to inactivate or phagocytosis of bacteria was significantly decreased, and the absence of SCR-3 protein also weakened phagocytes' ability to degrade bacteria and their metabolites. Furthermore, animal model of inflammatory reaction was established and the immune function was determined, and the results revealed that SRC-3 protein may play an important role in maintenance of T-cells' immune function, and severe T-cell immune function disorder would be resulted once SRC-3 protein is missing. In addition, the results of our study showed the steady-state of lymphocyte subsets was destroyed after SIRS, leading the suppression of cellular immune function, and the absence of SCR-3 protein may aggravate the suppression of T-lymphocyte function. Therefore, the present study demonstrated that the absence of SCR-3 protein would aggravate immunosuppression. In addition, SRC-3 protein is a significant regulator of infection and inflammation, and SRC-3 protein play an essential role in the development of immunosuppression accompanied with SIRS. © Springer Science+Business Media, LLC. 2011.


Li J.,General Hospital of Chengdu Military Command Area | Che J.-X.,The 44th Hospital of PLA | Bai S.-R.,General Hospital of Chengdu Military Command Area | Qiu Q.-M.,The 44th Hospital of PLA | And 3 more authors.
PLoS ONE | Year: 2013

Liver regeneration is the basic physiological process after partial hepatectomy (PH), and is important for the functional rehabilitation of the liver after acute hepatic injury. This study was designed to explore the effects of neurolytic celiac plexus block (NCPB) on liver regeneration after PH. We established a model of PH in rats, assessing hepatic blood flow, liver function, and serum CRP, TNF-α, IL-1β and IL-6 concentrations of the residuary liver after PH. Additionally, histopathological studies, immunohistochemistry, and western blotting were also performed. Our results indicated that NCPB treatment after PH improved liver regeneration and survival rates, increased hepatic blood flow, reduced hepatocyte damage, decreased the secretion and release of inflammatory cytokines, increased the expression of B cell lymphoma/leukemia-2 (Bcl-2), and decreased the expression of Bcl-2 associated X protein (Bax). Additionally, Western blotting revealed that the expression of NF-κB p65 and c-Jun were decreased in liver after NCPB. In conclusion, the results of our present study indicate that NCPB treatment has a favorable effect on liver regeneration after PH. We suggest that NCPB can be utilized as an effective therapeutic method to help the functional rehabilitation of the liver after acute hepatic injury or liver cancer surgery. © 2013 Li et al.

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