General Hospital of Beijing Military Area Command

Beijing, China

General Hospital of Beijing Military Area Command

Beijing, China

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Shen T.,Chongqing Medical University | Qiu L.,General Hospital of Beijing Military Area Command | Chang H.,Chongqing Medical University | Yang Y.,Chongqing Medical University | And 4 more authors.
International Journal of Clinical and Experimental Pathology | Year: 2014

Orthodontic forces result in alveolar bone resorption and formation predominantly on the pressure and tension sides of the tooth roots, respectively. Human periodontal ligament stem cells (PDLSCs) have demonstrated the capacity to differentiate into osteoblasts, and they play important roles in maintaining homeostasis and regenerating periodontal tissues. However, little is known about how PDLSCs contribute to osteoblastogenesis during orthodontic tooth movement on the tension side. In this study, we applied a 12% cyclic tension force to PDLSCs cultured in osteoinductive medium. The osteogenic markers Runx2, ALP, and OCN were detected at the mRNA and protein levels at different time points using real-time PCR and western blot analyses. We discovered that the mRNA and protein levels of Runx2, ALP and OCN were significantly up-regulated after 6, 12 and 24 hours of mechanical loading on PDLSCs compared to levels in unstimulated PDLSCs (P < 0.05). This study demonstrates, for the first time, the effects of mechanical tensile strain on the osteogenic differentiation of PDLSCs, as examined with a Flexcell FX-4000T Tension Plus System. Our findings suggested that cyclic tension could promote the osteogenic differentiation of PDLSCs. Furthermore, the effects of orthodontic force on alveolar bone remodeling might be achieved by PDLSCs.


Wang D.-W.,General Hospital of Beijing Military Area Command | Wang D.-W.,Chinese PLA General Hospital | Zhou R.-B.,General Hospital of Beijing Military Area Command | Yao Y.-M.,General Hospital of Beijing Military Area Command | And 6 more authors.
Journal of Pharmacology and Experimental Therapeutics | Year: 2010

α7 Nicotinic acetylcholine receptor (α7 nAChR) has been found in several non-neuronal cells and is described as an important regulator of cellular function. Naturally occurring CD4+CD25+ regulatory T cells (Tregs) are essential for the active suppression of autoimmunity. The present study investigated whether naturally occurring Tregs expressed α7 nAChR and investigated the functionary role of this receptor in controlling suppressive activity of these cells. We found that CD4 +CD25+ Tregs from naive C57BL/6J mice positively expressed α7 nAChR, and its activation by nicotine enhanced the suppressive capacity of Tregs. Nicotine stimulation up-regulated the expression of cytotoxic T-lymphocyte-associated antigen (CTLA)-4 and forkhead/winged helix transcription factor p3 (Foxp3) on Tregs but had no effect on the production of interleukin (IL)-10 and transforming growth factor-β1 by Tregs. In the supernatants of CD4+CD25+ Tregs/CD4+CD25 - T-cell cocultures, we observed a decrease in the concentration of IL-2 in nicotine-stimulated groups, but nicotine stimulation had no effect on the ratio of IL-4/interferon (IFN)-γ, which partially represented T-cell polarization. The above-mentioned effects of nicotine were reversed by a selective α7 nAChR antagonist, α-bungarotoxin. In addition, the ratio of IL-4/IFN-γ was increased by treatment with α-bungarotoxin. We conclude that nicotine might increase Treg-mediated immune suppression of lymphocytes via α7 nAChR. The effect is related to the up-regulation of CTLA-4 as well as Foxp3 expression and decreased IL-2 secretion in CD4 +CD25+ Tregs/ CD4+CD25- T-cell coculture supernatants. α7 nAChR seems to be a critical regulator for immunosuppressive function of CD4+CD25+ Tregs. Copyright © 2010 by The American Society for Pharmacology and Experimental Therapeutics.


Zhang D.,PLA Fourth Military Medical University | Ding Y.,General Hospital of Beijing Military Area Command | Wang Z.,The No 113 Hospital Of Pla | Wang Y.,PLA Fourth Military Medical University | Zhao G.,PLA Fourth Military Medical University
Tumor Biology | Year: 2015

A T>G single nucleotide polymorphism (SNP, rs2279744) of the MDM2 gene has been investigated in sarcoma community, but the findings are conflicting. This study was designed to well define the relationship between SNP rs2279744 and sarcoma risk. We did a systematic computerized search of the PubMed, Web of Science, and Science Direct databases to identify the human case–control studies investigating the relationship between SNP rs2279744 and sarcoma risk with complete genetic data. Pooled odds ratios (ORs) were calculated with the Mantel–Haenszel fixed-effect model or the DerSimonian and Laird random effects model to estimate the risk of sarcoma. Overall analysis included five independent studies. On the whole, the T/G genotype or the combined G/G and T/G genotypes appeared to be associated with approximately 1.40-fold higher risk of sarcoma relative to the T/T genotype (T/G vs. T/T: OR 1.33, 95 % CI 1.00–1.77; G/G + T/G vs. T/T: OR 1.42, 95 % CI 1.08–1.85). We noted that the Caucasian populations showed a similarly increased risk of sarcoma ascribed to the carriage of the same genotypes (T/G vs. T/T: OR 1.41, 95 % CI 1.05–1.90; G/G + T/G vs. T/T: OR 1.49, 95 % CI 1.13–1.97). This meta-analysis provides evidence that MDM2 SNP rs2279744 may be significantly associated with increased risk of sarcoma in Caucasian individuals. © 2014, International Society of Oncology and BioMarkers (ISOBM).


Ren S.,National Center for Clinical Laboratories and Beijing Hospital | Hu J.,National Center for Clinical Laboratories and Beijing Hospital | Chen Y.,General Hospital of Beijing Military Area Command | Yuan T.,National Center for Clinical Laboratories and Beijing Hospital | And 2 more authors.
Clinical and Experimental Immunology | Year: 2016

Inflammation instigated by interleukin (IL)-17-producing cells is central to the development and pathogenesis of several human autoimmune diseases and animal models of autoimmunity. The expansion of IL-17-producing cells from healthy donors is reportedly promoted by mesenchymal stem cells derived from fetal bone marrow. In the present study, human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) were examined for their effects on lymphocytes from healthy donors and from patients with systemic lupus erythematosus (SLE). Significantly higher levels of IL-17 were produced when CD4+ T cells from healthy donors were co-cultured with hUC-MSCs than those that were cultured alone. Blocking experiments identified that this effect might be mediated partially through prostaglandin E2 (PGE2) and IL-1β, without IL-23 involvement. We then co-cultured hUC-MSCs with human CD4+ T cells from systemic lupus erythematosus patients. Ex-vivo inductions of IL-17 by hUC-MSCs in stimulated lymphocytes were significantly higher in SLE patients than in healthy donors. This effect was not observed for IL-23. Taken together, our results represent that hUC-MSCs can promote the IL-17 production from CD4+ T cells in both healthy donor and SLE patients. PGE2 and IL-1β might also be partially involved in the promotive effect of hUC-MSCs. © 2016 British Society for Immunology.


Chhen Y.Q.,General Hospital of Beijing Military Area Command
Zhongguo gu shang = China journal of orthopaedics and traumatology | Year: 2011

To explore the features of rats organs functional changes after femur shaft fracture combined with brain injury through testing biochemical indicators in rats. Thirty 4-month-old male SD rats, weight (280 +/- 10) g, were divided into 5 groups according to random number table involving normal control group, at the 1st day of injured group, the 2nd day injured group, the 3rd day injured group, the 5th day injured group, the 7th day injured group, 5 rats in each group. The animal injury model of right femur shaft fracture combined with brain injury were made by instruments in 5 injured groups. At the 1st, 2nd, 3rd, 5th, 7th day after made model, the biochemical indicators of blood serum from abdominal aorta including AST, ALT ,Cr, BUN, LDH and CK were detected and compared. The biochemical indicator of blood serum (AST, ALT, Cr, BUN, LDH, CK) changed significantly among different groups. AST, ALT, BUN and CK reached peaks at the 1st day after injured (P<0.05). Cr reached peaks at the 3rd day after injured (P<0.05). LDH reached peak at the 2nd day after injured (P<0.05). After brain injury combined with right femoral fractures in rats, a variety of serum biochemical indicators increase significantly, especially in early 3 days after injured. These indicators shows indirectly heart, liver and kidney organ dysfunction by trauma.


Geng P.,Chinese People's Liberation Army | Ding Y.,General Hospital of Beijing Military Area Command | Qiu L.,General Hospital of Beijing Military Area Command | Lu Y.,Beijing 302 Hospital
Diabetes care | Year: 2015

OBJECTIVE: Inflammation and complement activation initiated by mannose-binding lectin (MBL) may be implicated in the pathogenesis of diabetic vascular complications. We investigated serum MBL levels in patients with diabetes with and without diabetic retinopathy (DR).RESEARCH DESIGN AND METHODS: Serum MBL levels were determined in 348 patients with diabetes and in 100 healthy control subjects. The prediction value of MBL was compared with diabetes duration, hs-CRP, and other known predictors. Multivariate analyses were performed using logistic regression models.RESULTS: MBL levels on admission were significantly increased in patients with diabetes with DR (P < 0.0001) and vision-threatening DR (VTDR; P < 0.0001). Multivariate logistic regression analysis adjusted for common indictors showed that serum MBL levels ≥3,385 μg/L were an independent predictor of DR (odds ratio [OR] 3.14, 95% CI 1.77-5.57) and VTDR (OR 7.83, 95% CI 3.35-18.31). The area under the receiver operating characteristic curve of MBL was 0.81 (95% CI 0.76-0.86) for DR and 0.84 (95% CI 0.74-0.93) for VTDR.CONCLUSIONS: The current study demonstrated that MBL appears to be an independent biomarker for DR in the Chinese population, suggesting a possible role of MBL in the pathogenesis of DR complications in diabetes. © 2015 by the American Diabetes Association. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered.


Men J.,Beijing Institute of Pharmacology and Toxicology | Lang L.,Beijing Institute of Pharmacology and Toxicology | Wang C.,Beijing Institute of Pharmacology and Toxicology | Wu J.,Beijing Institute of Pharmacology and Toxicology | And 4 more authors.
Analytical Biochemistry | Year: 2010

This work aimed to evaluate a method to detect the residual ricin in animal tissues. Immunoprecipitation and sandwich enzyme-linked immunosorbent assay (ELISA) were used to detect ricin in the tissues of intoxicated mice. The monoclonal antibodies (Mabs) 4C13 and 3D74 were used to assay the whole ricin molecules via sandwich ELISA. Mab 4C13 was conjugated with Sepharose 4B to capture ricin or ricin A chain by immunoprecipitation. Mice injected intravenously with ricin at the dosage of 5 μg/mouse were killed at different time points after intoxication. The serum, liver, kidney, lung, and intestine were harvested. High levels of ricin were found in serum and liver samples at each poisoning time point by sandwich ELISA, suggesting the possibility of determining ricin intoxication by detecting residual ricin in the serum. However, this method turned out to be ineffective for examining ricin in the kidney, lung, and intestine of poisoned mice. Although the same tissue samples of intoxicated mice were analyzed by immunoprecipitation, positive bands were found. This indicated that some components in the kidney, lung, and intestine could bind with ricin and interfere in its binding activity with the coated antibody. Immunoprecipitation could be used to measure the existence of ricin in these samples. © 2010 Elsevier Inc.


Geng P.,Wuhan University | Geng P.,Chinese People's Liberation Army | Geng P.,Beijing Tsinghua Changgung Hospital | Geng P.,Beijing 302 Hospital | And 3 more authors.
Diabetes Care | Year: 2015

OBJECTIVE: Inflammation and complement activation initiated by mannose-binding lectin (MBL) may be implicated in the pathogenesis of diabetic vascular complications. We investigated serum MBL levels in patients with diabetes with and without diabetic retinopathy (DR). RESEARCH DESIGN AND METHODS: Serum MBL levels were determined in 348 patients with diabetes and in 100 healthy control subjects. The prediction value of MBL was compared with diabetes duration, hs-CRP, and other known predictors. Multivariate analyses were performed using logistic regression models. RESULTS: MBL levels on admission were significantly increased in patients with diabetes with DR (P < 0.0001) and vision-threatening DR (VTDR; P < 0.0001). Multivariate logistic regression analysis adjusted for common indictors showed that serum MBL levels ≥3,385μg/L were an independent predictor of DR (odds ratio [OR] 3.14, 95% CI 1.77-5.57) and VTDR (OR 7.83, 95% CI 3.35-18.31). The area under the receiver operating characteristic curve of MBL was 0.81 (95% CI 0.76-0.86) for DR and 0.84 (95% CI 0.74-0.93) for VTDR. CONCLUSIONS: The current study demonstrated that MBL appears to be an independent biomarker for DR in the Chinese population, suggesting a possible role of MBL in the pathogenesis of DR complications in diabetes. © 2015 by the American Diabetes Association.


PubMed | General Hospital of Beijing Military Area Command, Beijing 302 Hospital and Chinese People's Liberation Army
Type: Journal Article | Journal: Diabetes care | Year: 2015

Inflammation and complement activation initiated by mannose-binding lectin (MBL) may be implicated in the pathogenesis of diabetic vascular complications. We investigated serum MBL levels in patients with diabetes with and without diabetic retinopathy (DR).Serum MBL levels were determined in 348 patients with diabetes and in 100 healthy control subjects. The prediction value of MBL was compared with diabetes duration, hs-CRP, and other known predictors. Multivariate analyses were performed using logistic regression models.MBL levels on admission were significantly increased in patients with diabetes with DR (P < 0.0001) and vision-threatening DR (VTDR; P < 0.0001). Multivariate logistic regression analysis adjusted for common indictors showed that serum MBL levels 3,385 g/L were an independent predictor of DR (odds ratio [OR] 3.14, 95% CI 1.77-5.57) and VTDR (OR 7.83, 95% CI 3.35-18.31). The area under the receiver operating characteristic curve of MBL was 0.81 (95% CI 0.76-0.86) for DR and 0.84 (95% CI 0.74-0.93) for VTDR.The current study demonstrated that MBL appears to be an independent biomarker for DR in the Chinese population, suggesting a possible role of MBL in the pathogenesis of DR complications in diabetes.


PubMed | National Center for Clinical Laboratories and Beijing Hospital, Capital Medical University and General Hospital of Beijing Military Area Command
Type: Journal Article | Journal: Clinical and experimental immunology | Year: 2016

Inflammation instigated by interleukin (IL)-17-producing cells is central to the development and pathogenesis of several human autoimmune diseases and animal models of autoimmunity. The expansion of IL-17-producing cells from healthy donors is reportedly promoted by mesenchymal stem cells derived from fetal bone marrow. In the present study, human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) were examined for their effects on lymphocytes from healthy donors and from patients with systemic lupus erythematosus (SLE). Significantly higher levels of IL-17 were produced when CD4(+) T cells from healthy donors were co-cultured with hUC-MSCs than those that were cultured alone. Blocking experiments identified that this effect might be mediated partially through prostaglandin E2 (PGE2 ) and IL-1, without IL-23 involvement. We then co-cultured hUC-MSCs with human CD4(+) T cells from systemic lupus erythematosus patients. Ex-vivo inductions of IL-17 by hUC-MSCs in stimulated lymphocytes were significantly higher in SLE patients than in healthy donors. This effect was not observed for IL-23. Taken together, our results represent that hUC-MSCs can promote the IL-17 production from CD4(+) T cells in both healthy donor and SLE patients. PGE2 and IL-1 might also be partially involved in the promotive effect of hUC-MSCs.

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