Pan B.,Peking University |
Ren H.,Peking University |
He Y.,Military General Hospital of Beijing |
Lv X.,Military General Hospital of Beijing |
And 9 more authors.
Clinical Cancer Research | Year: 2012
Purpose: Epidemiologic studies suggested complicated associations between type 2 diabetes mellitus and breast cancer. High-density lipoprotein (HDL) is inversely associated with the risk and mortality of breast cancer. Our study is to determine the different effects of normal and diabetic HDL on breast cancer cell metastasis. Experimental Design: MDA-MB-231 and MCF7 cells were treated with N-HDL, D-HDL, G-HDL, and Ox-HDL. Cell metastasis potency was examined using a tail-vein injection model, and cell adhesion abilities to human umbilical vein endothelial cells (HUVEC) and extracellular matrix (ECM) were determined in vitro. Integrin expression and protein kinase C (PKC) activity were evaluated, and PKC inhibitor was applied. Results: D-HDL dramatically promoted cell pulmonary metastasis (103.6% increase at P < 0.001 for MDA-MB-231 with 1 × 10 5 cell injection; 157.1% increase at P < 0.05 for MCF7 with 4 × 10 5 cell injection) and hepatic metastasis (18.1-fold increase at P < 0.001 for MCF7 with 4 × 10 5 cell injection), and stimulated higher TC-HUVECs adhesion (21.9% increase at P < 0.001 for MDA-MB-231; 23.6% increase at P < 0.05 for MCF7) and TC-ECM attachment (59.9% and 47.9% increase, respectively, for MDA-MB-231 and MCF7, both at P < 0.01) compared with N-HDL. D-HDL stimulated higher integrin (β1, β2, β3, and an) expression on cell surface and induced higher PKC activity. Increased TC-HUVECs and TC-ECM adhesion induced by D-HDL, G-HDL, and Ox-HDL could be inhibited by staurosporine. Conclusions: Our study showed that glycation and oxidation of HDL in diabetic patients could lead to abnormal actions on breast cancer cell adhesion to HUVECs and ECM, thereby promoting metastasis progression of breast cancer. This will largely draw the attention of HDL-based treatments in the diabetes patients with breast cancer. ©2012 AACR.
Yang W.,China Japan Friendship Hospital |
Lv X.,Military General Hospital of Beijing |
Li Q.,Chongqing Medical University |
Jia W.,Shanghai JiaoTong University |
Tian H.,University of Sichuan
Current Medical Research and Opinion | Year: 2012
Objective: To evaluate the efficacy, safety and treatment satisfaction of insulin glargine plus oral antidiabetic drugs (OADs) in Chinese individuals with Type 2 diabetes inadequately controlled with premixed insulin plus OADs. Methods: In this open-label, single-arm, 16-week, phase IV study, 313 subjects with Type 2 diabetes inadequately controlled with premixed insulin plus OADs were switched to insulin glargine plus OADs. Changes in glycaemic control, incidence of hypoglycaemia and treatment satisfaction using the Diabetes Treatment Satisfaction Questionnaire (DTSQ) were evaluated. Results: Switching to insulin glargine was associated with significant reductions in levels of glycosylated haemoglobin (HbA1c; 8.4±0.6 to 7.9±1.0%; p<0.001) and fasting plasma glucose (FPG; 9.50±2.10 to 6.58±2.07mmol/L; p<0.001). A total of 32.9% of subjects experienced hypoglycaemia, including two cases of severe hypoglycaemia. Treatment satisfaction was improved with insulin glargine (DTSQ 8-item scores, all p<0.001). Logistic regression analysis showed a significant association between baseline HbA1c, disease duration, endpoint FPG and HbA 1c<7%. Conclusion: This single-arm study suggested that switching to insulin glargine plus OADs significantly improved glycaemic control, with a low incidence of hypoglycaemia, in patients with Type 2 diabetes uncontrolled on premixed insulin plus OADs. Switching to insulin glargine was also associated with better patient treatment satisfaction compared with previous treatment. The main limitations to this study are the open-label design and the lack of a control arm. © 2012 Informa UK Ltd.
Wang X.,Sino Japan Friendship Hospital |
Li Z.,University of California at Los Angeles |
Liu Y.,306 Hospital Of Pla |
Lv X.,Military General Hospital of Beijing |
Yang W.,Sino Japan Friendship Hospital
Nutrition Journal | Year: 2012
Background: Studies have shown that pistachios can improve blood lipid profiles in subjects with moderate hypercholesterolemia which could reduce the risk of cardiovascular disease. However, there is also a widely perceived view that eating nuts can lead to body weight gain due to their high fat content. Purpose. To investigate the impact of different dosages of pistachios on body weight, blood pressure, blood lipids, blood glucose and insulin in subjects with metabolic syndrome. Methods. Ninety subjects with metabolic syndrome (consistent with 2005 International Diabetes Federation metabolic syndrome standard without diabetes) were enrolled in three endocrinology outpatient clinics in Beijing. All subjects received dietary counseling according to the guidelines of the American Heart Association Step I diet. After a 4 week run-in, subjects were randomized to consume either the recommended daily serving of 42 g pistachios (RSG), a higher daily serving of 70 g pistachio (HSG) or no pistachios (DCG) for 12 weeks. Results: Subjects in all three groups were matched at baseline for BMI: DCG 28.03 4.3; RSG 28.12 3.22; and HSG 28.01 4.51 kg/m 2. There were no significant changes in body weight or BMI in any groups during the study nor any change from baseline at any time point in any group. During the entire study, there were no significant differences in waist-to-hip ratio among the groups or any change from baseline in any group (DCG -0.00 0.03, RSG -0.01 0.02 and HSG 0.01 0.04). There were no significant differences detected among groups in triglycerides, fasting glucose and 2 hour postprandial glucose following a 75 gram glucose challenge. Exploratory analyses demonstrated that glucose values 2 h after a 75 gm glucose challenge were significantly lower at week 12 compared with baseline values in the HSG group (-1.13 2.58 mmol/L, p = 0.02), and a similar trend was noted in the RSG group (-0.77 2.07 mmol/L, p = 0.06), while no significant change was seen in the DCG group (-0.15 2.27 mmol/L, p = 0.530). At the end of study, serum triglyceride levels were significantly lower compared with baseline in the RSG group (-0.38 0.79 mmol/L, p = 0.018), but no significant changes were observed in the HSG or DCG groups. Conclusion: Despite concerns that pistachio nut consumption may promote weight gain, the daily ingestion of either 42 g or 70 g of pistachios for 12 weeks did not lead to weight gain or an increase in waist-to-hip ratio in Chinese subjects with metabolic syndrome. In addition, pistachio consumption may improve the risk factor associated with the metabolic syndrome. © 2012 Wang et al; licensee BioMed Central Ltd.
Yi F.,Harbin Medical University |
He X.,Military General Hospital of Beijing |
He X.,Chinese PLA General Hospital |
Wang D.,Harbin Medical University
Neurochemical Research | Year: 2013
Mitochondrial dysfunction is implicated in pathogenesis of Parkinson's disease (PD). Lycopene, a member of the carotenoid family of phytochemicals, exerts its neuroprotective effects by reducing oxidative damage and improving mitochondrial function in several experimental models. In an attempt to clarify the protective effect of lycopene on toxin-insulted dopaminergic neuronal death, the present study was carried out by using a typical PD-1-methyl-4- phenylpyridinium iodide (MPP+)-induced dopaminergic SH-SY5Y cellular model. SH-SY5Y cells were preincubated with different dose of lycopene for 2 h, followed by the challenge with 500 μM MPP+ for 24 h. It is found that lycopene attenuated MPP+-induced cytotoxicity, as evidenced by the improved cell viability and the decreased apoptotic rate. Additionally, lycopene suppressed the reactive oxygen species accumulation and lipid peroxidation caused by MPP+. Lycopene also ameliorated MPP +-induced mitochondria-derived ROS production and mitochondrial morphological changes. Furthermore, lycopene attenuated MPP+-induced opening of the mitochondrial permeability transition pore and the concomitant disruption of the mitochondrial membrane potential, reversed MPP +-induced reduction in ATP concentration and decreases in mitochondrial DNA copy numbers and mitochondrial RNA transcript levels. Together, the protective effects of lycopene against MPP+-induced cytotoxicity may be attributable to its roles in improving mitochondrial function. These data suggest that lycopene may provide a valuable therapeutic strategy for the treatment of PD. © 2013 Springer Science+Business Media New York.
Du H.-W.,Military General Hospital of Beijing |
Li J.-Y.,Chinese PLA General Hospital |
He Y.,Chinese PLA General Hospital
Journal of Geriatric Cardiology | Year: 2011
Backgroud: Numerous studies have confirmed the effectiveness of slowing the progression of atherosclerosis by blood pressure (Bp) control in patients with hypertension and several studies also showed the efficacy of intensive glycemic control in decreasing progression of carotid intima-media thickness (CIMT) in patients with type 1 and type 2 diabetes. However, few studies have compared the relative importance of glycemic vs. Bp control in patients with diabetes and hypertension. We aimed to investigate the association between Bp and glycemic control and subclinical carotid atherosclerosis in older patients with hypertension and type 2 diabetes. Methods: In a cross-sectional study, B-mode high-resolution ultrasonography of the carotid artery was performed in 670 subjects (508 males and 162 females) aged 60 years or over who had self-reported hypertension and diabetes but no history of coronary heart disease or stroke. Subjects were categorized by their systolic blood pressure: tight control, < 130 mmHg; usual control, 130-139 mmHg; or uncontrolled, ≥ 140 mmHg, and by their hemoglobin A1c (HbA1c) level: tight control, < 6.5%; usual control, 6.5%-7.5%; or uncontrolled, ≥ 7.5%, respectively. Results: The mean CIMT was 8.20 ± 0.11 mm, and carotid plaque was found in 52.5% (352/670) subjects. Overall, 62.1% of the subjects had subclinical carotid atherosclerosis, defined as having either carotid plaque or elevated CIMT (≥ 1.1 mm). The mean CIMT was significantly different between Bp control categories (7.60 ± 0.09 mm, 7.90 ± 0.08 mm, and 8.60 ± 0.12 mm, respectively, P = 0.03) but not between glycemic control categories (8.20 ± 0.10 mm, 8.1 ± 0.08 mm, and 8.40 ± 0.14 mm, respectively, P = 0.13) using ANCOVA analysis. Multivariable logistic regression adjusting for potential confounding factors showed that usual or uncontrolled Bp control were associated with having carotid plaque (OR = 1.08 and OR = 1.42, respectively), or elevated CIMT [Odd ratio (OR) = 1.17, 95% confidence interval (CI) 1.04-2.24, and OR = 1.54, 95% CI 1.36-2.96, respectively compared to tight Bp control; but did not show glycemic control as independent predictor of either having carotid plaque or elevated CIMT. Conclusions: In older patients with hypertension and diabetes, blood pressure control, but not glycemic control is associated with subclinical carotid atherosclerosis. ©2011 IGC All rights reserved.
Zhang Y.,Military General Hospital of Beijing |
Tong Z.,Military General Hospital of Beijing |
Zhang Y.,Wake forest University
Journal of X-Ray Science and Technology | Year: 2013
Splenogonadal fusion is a rare congenital anomaly where the spleen is attached to either testicular or ovarian tissues. In present case, splenogonadal fusion presents as a large mass consciously connected to atrophic testis in left scrotum. The diagnosis of splenic gonadal fusion relies on radiologic and pathologic findings. Uniform density and vessels connection originating from splenic hilum between principle spleen and let testicle were observed with ultrasound and X-ray computed tomography (CT). Surgical exploration ruled out malignancy, extratesticular scrotal mass with affected testicle was removed. Pathologic examination demonstrated that ectopic spleen tissue and ipsilateral atrophic testis. Our data suggested that orchiectomy should be performed when contralateral testicle is normal and the affected testicular appeared significantly atrophy. © 2013 - IOS Press and the authors. All rights reserved.
Bian X.,Military General Hospital of Beijing |
Chen H.,Fuzhou General Hospital |
Liao L.,Fujian University of Traditional Chinese Medicine
International Journal of Clinical Oncology | Year: 2012
Background Nasopharyngeal carcinoma is sensitive to radiotherapy. When there is local relapse, re-irradiation treatment is inevitably associated with serious complications and decreased quality of life. Surgical resection offers an alternative treatment option with acceptable morbidity. Methods Seventy-one consecutive patients with primary recurrence of nasopharyngeal carcinoma after radiation underwent nasopharyngectomy from January 1, 1990 to June 30, 2006. Follow-up ranged from 12 to 127 months. Results The actuarial 1-, 2-, 3-, and 5-year survival rates were 88.1, 62.1, 48.9, and 42.1%, respectively. The 1-, 2-, 3-, and 5-year local control rates were 74.6, 61.9, 56.3, and 53.5%, respectively. There was no surgical mortality. The 3-year overall survival rates for recurrent T1, T2, T3, and T4 disease after surgery were 56, 61.1, 30.6, and 0%, respectively; the corresponding 5-year overall survival rates were 49.1, 24.7, 0, and 0%, respectively. Other prognostic factors with a negative effect on survival include lymph node metastasis, invasion of skull base and parapharyngeal space, and positive margin. Conclusions Advances in skull base surgery make possible the effective control of primary recurrence of nasopharyngeal carcinoma for patients with rT1 and rT2 stages, with acceptable mortality and morbidity. © 2011 CARS.
Sun Y.,Military General Hospital of Beijing |
Shuang F.,94 Hospital of PLA |
Chen D.M.,Military General Hospital of Beijing |
Zhou R.B.,Military General Hospital of Beijing
Osteoporosis International | Year: 2013
Treatment with molecular hydrogen alleviates microgravity-induced bone loss through abating oxidative stress, restoring osteoblastic differentiation, and suppressing osteoclast differentiation and osteoclastogenesis. Introduction: Recently, it has been suggested that hydrogen gas exerts a therapeutic antioxidant activity by selectively reducing cytotoxic reactive oxygen species (ROS). The aim of the present study was to elucidate whether treatment with molecular hydrogen alleviated bone loss induced by modeled microgravity in rats. Methods: Hindlimb suspension (HLS) and rotary wall vessel bioreactor were used to model microgravity in vivo and in vitro, respectively. Sprague-Dawley rats were exposed to HLS for 6 weeks to induced bone loss and simultaneously administrated with hydrogen water (HW). Then, we investigated the effects of incubation with hydrogen-rich medium (HRM) on MC3T3-E1 and RAW264.7 cells exposed to modeled microgravity. Results: Treatment with HW alleviated HLS-induced reduction of bone mineral density, ultimate load, stiffness, and energy in femur and lumbar vertebra. Treatment with HW alleviated HLS-induced augmentation of malondialdehyde content and peroxynitrite content and reduction of total sulfhydryl content in femur and lumbar vertebra. In cultured MC3T3-E1 cells, incubation with HRM inhibited modeled microgravity-induced ROS formation, reduction of osteoblastic differentiation, increase of ratio of receptor activator of nuclear factor kappa B ligand to osteoprotegerin, inducible nitric oxide synthetase upregulation, and Erk1/2 phosphorylation. In cultured RAW264.7, incubation with HRM aggravated modeled microgravity-induced ROS formation, osteoclastic differentiation, and osteoclastogenesis. Conclusion: Treatment with molecular hydrogen alleviates microgravity-induced bone loss in rats. Molecular hydrogen could thus be envisaged as a nutritional countermeasure for spaceflight but remains to be tested in humans. © 2012 International Osteoporosis Foundation and National Osteoporosis Foundation.
Wang X.B.,Military General Hospital of Beijing
Zhonghua xin xue guan bing za zhi | Year: 2010
To investigate the anti-atherotic effects of heme-L-lysinate in a rabbit model of atherosclerosis and related machanisms. Adult rabbits were treated with 1% cholesterol diet (chol group, n = 8) or 1% cholesterol diet plus heme-L-lysinate (9 mgxkg(-1)xd(-1), Heme group, n = 8) or 1% cholesterol diet plus isotonic Na chloride (Na chloride group, n = 8) for 10 weeks. Eight rabbits fed with normal diet served as normal control. Aortic carbon monoxide (CO) was quantified spectrophotometrically by the formation of carboxyhaemoglobin (HbCO). Aortic heme oxygenase-1 (HO-1) and HSP70 mRNA and protein expressions were detected by RT-PCR and immunohistochemical staining. Aortic CO production and HO-1 activity were significantly increased in chol group and Na chloride group compared those in normal control group (P < 0.01). Aortic plaque area was significantly reduced in heme group (26.6% +/- 9.2%) than that in chol group (42.3% +/- 8.7%, P < 0.01). Aortic HO-1 expression, CO production and HSP70 were significantly increased in heme group than those in chol group and Na chloride group (all P < 0.01). Heme-L-lysinate could attenuate atherosclerotic progression through upregulating HO-1 and HSP70 expression and increasing CO production in this model.
Zhou J.,Military General Hospital of Beijing
Brazilian Journal of Medical and Biological Research | Year: 2016
Autologous blood transfusion (ABT) has been gradually attracting more attention due to the increasingly prominent problem of blood transfusion safety and blood shortage in recent years. With the rapid development of blood conservation techniques, blood component separation technology, blood transfusion medicine and a constant increase in clinical needs, ABT technology has been expanded and innovated to a large degree. In this study, the development of preoperative autologous blood donation (PABD), acute normovolemic hemodilution (ANH), intraoperative and postoperative autotransfusion, and other new technologies and theories are reviewed and existing questions are analyzed. Challenges and applications are also discussed in order to provide reference for peers. © 2016, Associacao Brasileira de Divulgacao Cientifica. All rights reserved.