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Hao T.,General Hospital of Beijing Command
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery | Year: 2010

To investigate the effectiveness of autogenous platelet-rich plasma (PRP) gel with acellular xenogeneic dermal matrix in the treatment of deep II degree burns. From January 2007 to December 2009, 30 cases of deep II degree burns were treated. There were 19 males and 11 females with an average age of 42.5 years (range, 32-57 years). The burn area was 10% to 48% of total body surface area. The time from burn to hospitalization was 30 minutes to 8 hours. All patients were treated with tangential excision surgery, one side of the wounds were covered with autogenous PRP gel and acellular xenogeneic dermal matrix (PRP group), the other side of the wounds were covered with acellular xenogeneic dermal matrix only (control group). The healing rate, healing time, infection condition, and scar formation were observed. At 7 days after operation, the infection rate in PRP group (6.7%, 2/30) was significantly lower than that in control group (16.7%, 5/30, P < 0.05). The healing times were (18 +/- 4) days and (22 +/- 4) days respectively in PRP group and control group, showing significant difference (P < 0.05). The healing rates at 14 days and 21 days were 75% +/- 7% and 88% +/- 5% in PRP group, were 62% +/- 15% and 73% +/- 7% in control group, showing significant difference (P < 0.05). RPR group was superior to control group in elasticity, color, appearance, softness, scar formation, and healing quality. Autogenous PRP gel with acellular xenogeneic dermal matrix can accelerate the wound healing of deep II degree burns as well as alleviate the scar proliferation.

Zhou J.,PLA Fourth Military Medical University | Yang J.,PLA Fourth Military Medical University | Fan X.,PLA Fourth Military Medical University | Hu S.,PLA Fourth Military Medical University | And 12 more authors.
Autophagy | Year: 2016

ABSTRACT: LAMP2A is the key protein of chaperone-mediated autophagy (CMA), downregulation of LAMP2A leads to CMA blockade. CMA activation has been implicated in cancer growth, but the exact mechanisms are unclear. Elevated expression of LAMP2A was found in 8 kinds of tumors (n=747), suggesting that LAMP2A may have an important role in cancer progression. Unsurprisingly, LAMP2A knockdown in gastric cancer (GC) cells hindered proliferation, accompanied with altered expression of cell cycle-related proteins and accumulation of RND3/RhoE. Interactomic and KEGG analysis revealed that RND3 was a putative CMA substrate. Further study demonstrated that RND3 silencing could partly rescue the proliferation arrest induced by LAMP2A knockdown; RND3 was increased upon lysosome inhibition via both chemicals and LAMP2A-shRNA; Furthermore, RND3 could interact with CMA components HSPA8 and LAMP2A, and be engulfed by isolated lysosomes. Thus, constant degradation of RND3 by CMA is required to sustain rapid proliferation of GC cells. At last, the clinical significance of LAMP2A was explored in 593 gastric noncancerous lesions and 173 GC tissues, the results revealed that LAMP2A is a promising biomarker for GC early warning and prognosis of female GC patients. © 2016 Taylor & Francis.

Zou Z.,University of South China | Jiang X.,University of South China | Zhang W.,University of South China | Zhou Y.,University of South China | And 3 more authors.
Brain Research | Year: 2010

The aim of this study is to determine the efficacy of injecting adult bone marrow derived stem cells (BMSCs) transfected with a pEGFP-C2 plasmid containing the gene for Tyrosine Hydroxylase (TH) into the lateral ventricle for treating rats with Parkinson's Disease (PD) induced by injections into the Substantia Nigra pars compacta (SNc) with 6-hydroxydopamine (6-OHDA), a potent and selective neurotoxin for catecholamine expressing neurons. BMSCs were obtained from the femur of rats; transfected with plasmid constructed with TH and green fluorescent protein (GFP) (with about 85% co-transfection efficiency rate) and then cultured with neuronal differentiation media. Eighty rats were injected into the SNc with 6-OHDA and tested behaviorally to verify the model was induced. Then, 12 PD rats were injected into the anterior horn of the lateral ventricle with x105 cells, while 12 more rats were given saline as control. We found that 10 days after transplantation there was a significant (P < 0.01) reduction in Apomorphine induced rotations in rats receiving transplanted cells. Also, combined SNc and Striatal dopamine contents (μg/g wet tissue weight) in transplanted rats were greater than controls (0.19 ± 0.06 vs 0.63 ± 0.14 P < 0.01). Immunohistological examination found GFP expression, indicating the presence of transplanted cells within the brain, some of which had migrated through the nerve fibers along the ventricular wall. We feel this study shows the efficacy of genetically engineered BMSCs in the treatment of a rat model of PD. However, future experiments are needed to determine the mechanisms.

Yan H.,General Hospital of Beijing Command | Chen Z.,Headquarter of Hospital | Sun S.-G.,General Hospital of Beijing Command | Zhai S.-D.,Peking University
Medical Journal of Chinese People's Liberation Army | Year: 2014

Objective To comprehend the development status quo of the management specialty of high-risk medications, to elucidate the trend on relational research field, and to provide a theoretical basis for rational clinical medication. Methods The literature published domestically and abroad was reviewed, the development status on the management specialty of high-risk medications was reviewed, an idea of future development on the research field of high-risk medications was formed, and responsibility of clinicians on high-risk medication management was elucidated. Results The research work of high-risk medication management is still in its infancy in China. Its studies were dispersed in certain areas, with remarkable repetition, and large gaps were found in the research field as compared with that in developed countries. Development of the said discipline and its related research should be strengthened at the present and henceforth. The corresponding society should play a leading role in the development of the discipline. Systemic analysis and comprehensive evaluation should be implemented by applying pharmaceutical epidemiology and evidence-based pharmacology methodology. The catalogue of high-risk medications and the evidence-based guideline of injury prevention against high-risk medications should be established, and the control regulation of high-risk medications should be formulated. The research achievements should be assessed and improved, and then disseminated and applied. An integrated and scientific theoretical system of high-risk medications management should be established, which should contain institutional framework, education, research, and management regulation. Conclusions A joint participation of several disciplines and professions is needed in the research work of high-risk medications management in order to guide the safe and rational use of the drugs. The responsibilities of different professionals, including physicians, pharmacists, nurses and administrator should be stressed in the research work of high-risk medications management in order to raise the safety and optimal use of the drugs.

Gong J.,General Hospital of Beijing Command | Zhang Y.,Chinese Institute of Aviation Medicine | Yang Z.,Chinese Institute of Basic Medical Sciences | Huang Y.,General Hospital of Beijing Command | And 2 more authors.
Psychology, Health and Medicine | Year: 2013

The framing effect, identified by Tversky and Kahneman, is one of the most striking cognitive biases, in which people react differently to a particular choice depending whether it is presented as a loss or as a gain. Numerous studies have subsequently demonstrated the robustness of the framing effect in a variety of contexts, especially in medical decision-making. Compared to daily decisions, medical decisions are of low frequency but of paramount importance. The framing effect is a well-documented bias in a variety of studies, but research is inconsistent regarding whether and how variables influence framing effects in medical decision-making. To clarify the discrepancy in the previous literature, published literature in the English language concerning the framing effect was retrieved using electronic and bibliographic searches. Two reviewers examined each article for inclusion and evaluated the articles' methodological quality. The framing effect in medical decision-making was reviewed in these papers. No studies identified an influence of framing information upon compliance with health recommendations, and different studies demonstrate different orientations of the framing effect. Because so many variables influence the presence or absence of the framing effect, the unexplained heterogeneity between studies suggests the possibility of a framing effect under specific conditions. Further research is needed to determine why the framing effect is induced and how it can be precluded. © 2013 Taylor & Francis.

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