Time filter

Source Type

Cui G.,Peking University | Leng H.,Peking University | Wang K.,Peking University | Wang J.,Peking University | And 6 more authors.
PLoS ONE | Year: 2013

This study aims to evaluate the effects of Remifemin (isopropanolic extract of Cimicifuga Racemosa) on postmenopausal osteoporosis. 120 female Sprague-Dawley rats were randomly assigned to four groups: sham surgery with vehicle, ovariectomy with vehicle, ovariectomy with estradiol valerate, or ovariectomy with Remifemin. Daily oral administrations of the vehicle, estradiol valerate, or Remifemin began 2 weeks after surgery and lasted to 4, 8, or 12 weeks. Ten rats in each group were sacrificed at each timestep with assessment of bone mineral density, trabecular bone structure, and biomechanical parameters of the femur and lumbar vertebra. Bone turnover markers were evaluated 12 weeks after surgery. Both drugs prevented bone density loss in the distal end of the femur and preserved the trabecular bone structure in both the lumbar vertebra and distal end of the femur following ovariectomy. Both drugs protected bone stiffness at the tested regions and reduced bone reabsorption in ovariectomized rats. The preventive effects of Remifemin against bone-loss can rival those of estradiol valerate if treatment duration is adequately extended. In conclusion, Remifemin may demonstrate equivalent effects to estradiol valerate in terms of preventing postmenopausal osteoporosis. Copyright: © 2013 Cui et al.


Yang L.,Peking University | Chen X.,Peking University | Liu H.,Hebei Yanda Hospital | Chen Y.,Zunyi Medical College Affiliated Hospital | And 3 more authors.
Transplant Immunology | Year: 2015

We investigated the clinical characteristics of acute lymphoblastic leukemia (ALL) patients undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT) using haploidentical donors carrying HLA-B*:01/. TNFα - 308A (B*:01-TNF2) haplotype. A total of 136 B*:01-TNF2 haplotype in related donors was determined B*:01-TNF2 haplotype (21 cases), those using haploidentical donors without B*:01-TNF2 haplotype (100 cases), and those using HLA-identical siB*:01-TNF2 haplotype (44 cases). Compared with the other two groups, patients using haploidentical donors carrying B*:01-TNF2 haplotype had higher overall mortality (adjusted P= 0.039) and non-relapse mortality (adjusted P= 0.001) within 2. years, delayed platelet engraftment (adjusted P<. 0.0001), higher incidences of severe acute graft-versus-host disease (aGVHD) (P= 0.007), severe late-onset hemorrhagic cystitis (P= 0.002), B*:01-TNF2 haplotype may cause more serious complications and poorer outcomes to ALL recipients. © 2014 Elsevier B.V.


Yang L.,Beijing 301 Hospital | Yang L.,General Hospital of Armed Police | Liu H.,Beijing Daopei Hospital | Zhao J.,Peking University | And 5 more authors.
Leukemia Research | Year: 2011

To address whether mutations and single nucleotide polymorphisms (SNPs) in perforin gene (PRF1) are correlated with acute lymphoblastic leukemia (ALL) in Chinese, we screened mutations in codon region of PRF1 in 111 ALL patients, and correlated the results with patients' immunophenotype, karyotype and fusion genes. Four novel monoallelic missense and two novel monoallelic synonymous mutations (G198R, R225Q, D486G, R509K, S388S and Q540Q) were identified in 9 B-ALL, of whom 7 cases carried BCR-ABL gene, one carried MLL-AF4 fusion gene, and one lost two chromosomes. Our results suggest that mutations in PRF1 may play a role in the pathogenesis of B-ALL. © 2010 Elsevier Ltd.


Sun X.,Capital Medical University | Ying J.,Puren Hospital of Beijing | Wang Y.,The First Hospital of DandongLiaoning | Li W.,Shanghai University | And 5 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

Aim: Meta-analysis on the effectiveness of the autologous stem cell transplantation in curing limb ischemic. Method: Consulting papers relate to the autologous stem cell transplantation in curing limb ischemic in PubMed, CNKI, Wan-fang Data and VIP. Based on include and exclude standards, we arrange at least 2 evaluators sifting these papers separately, doing Quality evaluation and information extraction and then cross checking. Negotiate through a third party if any disagreement comes out. Review Manager 4.2 is used in Meta-analysis. Result: Involved 7 papers, all in English version. Patients involved all diagnosed as critical limb ischemia (CLI). Results show that no adverse reaction occurred during this study. Amputation rate in patients with stem cell group treatment is lower than control group (P < 0.05). And no notable difference in improving ABI (P > 0.05). Conclusions: Applying autologous stem cell transplantation in curing limb ischemic does not have obviously effectiveness in the improvement of ABI of the limb ischemic patients. But it can dramatically reduce the rate of amputation. So autologous stem cell transplantation is a good and safe choice for patients have no choice but amputation. © 2015, E-Century Publishing Corporation. All rights reserved.


Wang W.,Peking University | Bai W.,Peking University | Cui G.,Peking University | Cui G.,Civil Aviation General Hospital | And 5 more authors.
Neuroendocrinology | Year: 2015

Aims: We investigated the norepinephrine pathway changes from the locus coeruleus (LC) to the preoptic area of the hypothalamus (POAH) in the brain of ovariectomized rats under low estrogen levels and explored the therapeutic effects of estradiol valerate (E2) and Remifemin (ICR) on these changes. Methods: 40 female Sprague-Dawley rats were randomly divided into the following groups: surgery with vehicle (SHAM), ovariectomy surgery with vehicle (OVX), ovariectomy with E2 treatment (OVX + E2), and ovariectomy with Remifemin (OVX + ICR). After 4 weeks of treatment, we observed the changes by immunohistochemistry. Results: (1) The average optical density of DBH-ir fibers and the number of α1-adrenoreceptor-and estrogen receptor (ER)α-positive neurons in the main nuclei of POAH were all reduced in OVX rats compared with the SHAM group. The above changes were normalized in all nuclei of the POAH in the E2 group, while they were normalized in some nuclei in the ICR group. Coexpression of ERα and α1-adrenoreceptor was observed in the POAH. (2) The number of DBH-and ERα-positive neurons in the LC decreased in the OVX group compared with the SHAM group and increased after treatment with E2 and ICR. Coexpression of ERα and DBH was observed in the LC. Conclusion: Low estrogen (OVX) altered norepinephrine synthesis in the LC, the projection of norepinephrine fibers and α1-adrenoreceptor expression in the POAH. Both E2 and ICR normalized the norepinephrine pathway, but E2 achieved greater effects than ICR. ICR had different effects in different nuclei in the POAH and its therapeutic effect was better in the LC. © 2015 S. Karger AG, Basel.


PubMed | General Hospital of Armed Police and Peking University
Type: Journal Article | Journal: PloS one | Year: 2013

This study aims to evaluate the effects of Remifemin (isopropanolic extract of Cimicifuga Racemosa) on postmenopausal osteoporosis. 120 female Sprague-Dawley rats were randomly assigned to four groups: sham surgery with vehicle, ovariectomy with vehicle, ovariectomy with estradiol valerate, or ovariectomy with Remifemin. Daily oral administrations of the vehicle, estradiol valerate, or Remifemin began 2 weeks after surgery and lasted to 4, 8, or 12 weeks. Ten rats in each group were sacrificed at each timestep with assessment of bone mineral density, trabecular bone structure, and biomechanical parameters of the femur and lumbar vertebra. Bone turnover markers were evaluated 12 weeks after surgery. Both drugs prevented bone density loss in the distal end of the femur and preserved the trabecular bone structure in both the lumbar vertebra and distal end of the femur following ovariectomy. Both drugs protected bone stiffness at the tested regions and reduced bone reabsorption in ovariectomized rats. The preventive effects of Remifemin against bone-loss can rival those of estradiol valerate if treatment duration is adequately extended. In conclusion, Remifemin may demonstrate equivalent effects to estradiol valerate in terms of preventing postmenopausal osteoporosis.


PubMed | General Hospital of Armed Police and Peking University
Type: Journal Article | Journal: Acta histochemica | Year: 2014

The purpose of this study was to investigate the difference in neuronal activity in the preoptic area of the hypothalamus (POAH) under low estrogen condition induced by ovariectomy. One hundred and twenty sham-operated (SHAM) and ovariectomized (OVX) rats were placed in different temperatures for 2h. Twelve rats from each group were stimulated by 4C, 10C, 25C, 33C and 38C, respectively. c-Fos expression in the POAH was detected by immunohistochemistry. Following exposure to warm and cold stimuli, there were markedly lower c-Fos-positive cell densities in the OVX group compared with the SHAM group in the median preoptic nucleus (MnPO) at 4C, 10C, 33C and 38C, in the medial preoptic area (MPA) at 25C and 38C, in the ventromedial preoptic nucleus (VMPO) at 4C, 10C and 38C and in the ventrolateral preoptic nucleus (VLPO) at 4C and 38C. Both temperature and surgery had an impact on c-Fos expression by two-way ANOVA method except in the lateral preoptic area (LPO). c-Fos expression differed within different nuclei of the two groups in the same and different temperature stimuli. This indicated that the temperature-sensitive nuclei in the POAH exhibited lower and different activities during temperature stimuli following ovariectomy, which possibly resulted in abnormal thermoregulation and menopausal symptoms.


Tian Y.-M.,No 474 Hospital Of Chinese Pla | Xiao L.-H.,General Hospital of Armed Police | Gao X.-W.,No 474 Hospital Of Chinese Pla
International Journal of Ophthalmology | Year: 2011

AIM: To assess features of cavernous hem angioma (CH) in the orbit revealed by CT and MRI and summarize prediction of preoperative CT and MRI for the adhesion degree of CH in the orbit. METHODSA: total of 97 patients with pathologically confirmed CH in the orbit were exam ined with axial and coronal CT scan, and axial, coronal, sagittal, and enhanced fat suppression MRI scan. CT and MRI findings and intraoperative adhesion degrees were retrospectively analyzed. RESULTS: There were 47 patients with slight adhesion, for whom CT and MRI showed round masses with well defined margins in the extraocular muscles; 1 4 patients with mild adhesion, for whom CT and MRI revealed irregular masses with unclear boundary between CH and the opticnerve in coronal images, and emissaryveins in the posterior region of masses in contrast-enhanced images; 36 patients with severe adhesion, for whom CT and MRI exhibited an irregular or ovoid mass filling the orbital apex, or showed distorted and even spiky margins in the posterior region of masses in contrast enhanced images at the presence of a transparent triangle between the mass and the orbital apex. CONCLUSION: Preoperative CT and MRI aid in accurate diagnosis, selection of the surgical approach, and assessment of the adhesion degree and surgicalrisks for CH. © International Journal of Ophthalmology Press.


Mu X.,Chinese PLA General Hospital | Mu X.,General Hospital of Armed Police | Wang H.,General Hospital of Armed Police | Ma Q.,General Hospital of Armed Police | And 2 more authors.
Academic Radiology | Year: 2014

Rationale and Objectives: The objective of this study was to determine the diagnostic accuracy of contrast-enhanced magnetic resonance angiography (MRA) when used in the preoperative evaluation of hepatic vascular anatomy in living liver donors. Materials and Methods: A computer-assisted literature searching of EMBASE, PubMed (MEDLINE), and the Cochrane library databases was conducted to identify potentially relevant articles which primarily examined the utility of contrast-enhanced MRA in the preoperative evaluation of hepatic vascular anatomy in living liver donors. We used the Q statistic of chi-squared value test and inconsistency index (I-squared, I2) to estimate the heterogeneity of the data extracted from all selected studies. Meta-Disc software (version 1.4) (ftp://ftp.hrc.es/pub/programas/metadisc/Metadisc_update.htm) was used to perform our analysis. Results: Eight studies were included in the present meta-analysis. A total of 289 living liver donor candidates and 198 patients who underwent liver harvesting were included in the present study. The pooled sensitivities of hepatic artery (HA), portal vein (PV), and hepatic vein (HV) in this meta-analysis were 0.84, 0.97, and 0.94, respectively. The pooled specificities of HA, PV, and HV were 1.00, 1.00, and 1.00, respectively. The pooled diagnostic odds ratios of HA, PV, and HV were 127.28, 302.80, and 256.59, respectively. The area under the summary receiver-operating characteristic curves of HA, PV, and HV were 0.9917, 0.9960, and 0.9813, respectively. Conclusions: The high sensitivity and specificity demonstrated in this meta-analysis suggest that contrast-enhanced MRA was a promising test for the preoperative evaluation of hepatic vascular anatomy in living liver donors. © 2014 AUR.


Wang H.,General Hospital of Armed Police | Wu C.-N.,General Hospital of Armed Police | Mu X.-T.,General Hospital of Armed Police
Chinese Journal of Medical Imaging Technology | Year: 2010

Objective: To compare 3D-SPACE-T2WI-MRC with Gd-BOPTA contrast enhanced MRC (CE-MRC) for evaluation on biliary anatomy and variation in liver transplant donor candidates. Methods: Totally 32 healthy liver transplant donor candidates underwent CE-MRC and 3D-SPACE-T2WI-MRC. According to intraoperative cholangiography, the diagnosing ability of biliary variation were analyzed and compared between two methods. Results: CE-MRC provided a superior visualization of the common bile duct, hepatic duct, left hepatic duct and right hepatic duct than 3D-SPACE-T2WI-MRC did (P<0.05), whereas no difference was found in displaying of the cystic duct, left anterior and posterior segment duct, right anterior and posterior segment duct and bile duct above the third grade branches between the two methods (P>0.05). Biliary variation was proved with intraoperative cholangiography in 17 cases, 14 of them were diagnosed with 3D-SPACE-T2WI-MRC, 15 with CE-MRC, while all were diagnosed correctly with combining application of these two methods. Conclusion: 3D-SPACE-T2WI-MRC and CE-MRC can be both used to evaluate biliary anatomy of liver transplant donor candidates, CE-MRC is superior to 3D-SPACE-MRC in display of part of the bile duct. The effect of combining application of these two methods is much better.

Loading General Hospital of Armed Police collaborators
Loading General Hospital of Armed Police collaborators