Echalier C.,Genepep Domaine des Pins |
Al-Halifa S.,Max Mousseron Institute of Biomolecules |
Kreiter A.,Genepep Domaine des Pins |
Enjalbal C.,Max Mousseron Institute of Biomolecules |
And 7 more authors.
Amino Acids | Year: 2013
Despite correct purity of crude peptides prepared on trityl resin by Fmoc/tBu microwave assisted solid phase peptide synthesis, surprisingly, lower yields than those expected were obtained while preparing C-terminal acid peptides. This could be explained by cyclization/cleavage through diketopiperazine formation during the second amino acid deprotection and third amino acid coupling. However, we provide here evidence that this is not the case and that this yield loss was due to high temperature promoted hydrolysis of the 2-chlorotrityl ester, yielding premature cleavage of the C-terminal acid peptides. © 2013 Springer-Verlag Wien.