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Human MECP2 gene located at q28 arm of X chromosome was identified as target for thermal co-amplification with HIV-1 proviral DNA of infected individuals. The selected MECP2 gene-specific primers functioned at a wide range of annealing temperature, extension time and exhibited no significant interaction with pathogen specific primers. A 466 bp PCR amplicon originating from human MECP2 gene was found to be diagnostic for inhibition-free PCR reaction when co-amplified with the HIV-1 target gene in a multiplexed, nested PCR reaction. The 5' end of the MECP2 primers were engineered to position an EcoRI restriction endonuclease site to facilitate rapid cloning in various DNA vector molecules at the corresponding EcoRI sites. Cell mass of Escherichia coli (XL1Blue) harboring the recombinant plasmid when added to pleural fluid of HIV-1 infected individuals co-infected with Mycobacterium tuberculosis, generated the diagnostic 466 bp MECP2 PCR amplicon as well as the 194 bp PCR amplicon of target gene from M. tuberculosis. The experiment underlined potential of the region spanning nucleotide position 4118099 to 4118552 of human MECP2 gene (NCBI accession number NT_011726.13) as a reliable target for multiplex PCR to accommodate a wide range of thermal cycling and multiplex reaction conditions. In both cases of this study, electrophoresis-based separation of the 466 bp MECP2 fragment and the 232 bp and 194 bp HIV-1 and M. tuberculosis fragments respectively was distinct and unambiguous. The potential of this human MECP2 gene available from human genome or recombinant plasmid as a potent target to monitor PCR inhibition for a range of different PCR reactions is discussed. Source

Kunjadia P.D.,Gujarat University | Kunjadia P.D.,M B Patel Science College | Kunjadia P.D.,Shri An Patel Pg Institute | Patel F.D.,M B Patel Science College | And 5 more authors.
BioResources | Year: 2012

The extracellular enzyme production capacity of Pleurotus ostreatus MTCC 142 was investigated for decolorization of crystal violet under solid and submerged conditions. Laccases are the major extracellular lignocellulolytic enzymes produced by fungus. Pleurotus ostreatus provided an effective decolorization of dye at 20 mg/L concentration up to 92%. Mycelial growth was observed maximum on plate for a dye concentration 20 mg/L while lowest on 200 mg/L on day 12, respectively. At all concentrations of dye studied, maximum laccase activity was observed on day 8. For 20 mg/L of dye laccase activity was 133 U/L. The decolorization was attributed to microbial action and without role of pH change; less than 0.4 pH change was observed. Manganese dependent peroxidase activity was 106 U/L, maximum on day 8 incubated with 20 mg/L dye concentration. The present study suggested that the high efficiency decolorization of crystal violet by P. ostreatus was assisted by laccase and manganese-dependent peroxidase activity and can be exploited as a promising in biological treatment of waste water containing crystal violet. Source

Kunjadia P.D.,M B Patel Science College | Kunjadia P.D.,B N Patel Institute Of Paramedical And Science | Nagee A.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science | Pandya P.Y.,M B Patel Science College | And 4 more authors.
International Journal of Medicinal Mushrooms | Year: 2014

Oyster mushrooms, species of the genus Pleurotus, are recognized for producing secondary metabolites with important medicinal properties. Investigations were carried out to evaluate the antioxidative and antimicrobial properties of the edible mushroom Pleurotus ostreatus (MTCC142) extracts cultivated on banana agrowastes. Ethanolic extracts showed antimicrobial activities against gram-positive and gram-negative bacteria, and their in vitro antifungal activities against all fungi tested revealed a promising role. Qualitative phytochemical analysis of Pleurotus grown on yeast dextrose broth and banana agrowaste confirmed the presence of steroids, cardiac glycosides, terpenoids, and alkaloids, whereas ethanolic extract after 40 days exhibited a phenol concentration of 521.67 μg/mL in banana waste compared to 155 μg/mL in yeast dextrose broth. The minimum inhibitory concentration of ethanolic extracts ranged from 19.74 to 56.84 mg/mL and 35.53 to 102.31 mg/mL in solid-state and submerged grown mycelium extracts, respectively, after 40 days. Moreover, banana agrowaste could be a significant economic source for the production of the oyster mushroom P. ostreatus. The nutritive, medicinal, and antimicrobial properties of P. ostreatus can be used to develop a new nutraceutical formulation; it can also be used as an additive to routine and fast food. © 2014 Begell House, Inc. Source

Mukhopadhyaya P.N.,geneOmbio Technologies | Acharya A.,geneOmbio Technologies | Chavan Y.,geneOmbio Technologies | Purohit S.S.,geneOmbio Technologies | Mutha A.,Diabetes Care and research Foundation
Genetics and Molecular Research | Year: 2010

A population-based study was undertaken to evaluate linkage between single-nucleotide polymorphisms known as risk factors and type 2 diabetes in an Indian population. The study population was comprised of 40 normal glucose-tolerant individuals (21 males and 19 females) and 40 type 2 diabetes patients (21 males and 19 females). The genes and their corresponding single-nucleotide polymorphisms that we screened were VDR (rs 731236 and rs 1544410), IL-6 (rs 1800795), TCF7L2 (rs 7903146) and TNF-α (rs 1800629). The risk alleles were more frequent in the subjects with type 2 diabetes, except for the TNF-α gene, which was very infrequent in the population; the normal allele occurred at high and similar frequencies in both normal and diabetic individuals. © FUNPEC-RP. Source

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