Gene Engineering Co.
Gene Engineering Co.
Hong J.-X.,Gene Engineering Co. |
Zhang Q.-Z.,Shanghai University |
Zhang Q.-Z.,Zhejiang Sci-Tech University |
Han J.-L.,Gene Engineering Co. |
And 5 more authors.
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010
BACKGROUND: In vitro culture condition and culture efficiency are different in reported umbilical cord-derived mesenchymal stem cells, and lacked of unified standards. Different derived mesenchymal stem cells have different biological properties. Therefore, it is very necessary to establish a simple and high-performance culture system for umbilical cord-derived mesenchymal stem cells. OBJECTIVE: To observe the growth state of human umbilical cord-derived mesenchymal stem cells in different culture systems in vitro and adenovirus infection efficiency. METHODS: Mesenchymal stem cells were separated from healthy full-termed delivery fetus using collagenase digestion method and purified by adherent culture. These cells were cultured and amplified in DMEM (low glucose), MesenPRO RS™ Medium and STEMPRO® MSC SFM in vitro. The 3-5 passage mesenchymal stem cells were infected by the Ad5-EGFP, Ad5/11-EGFP, Ad5/35-EGFP as multiplicity of infection (MOI)=1, 10, 100. Viral infection and green fluorescence expression were observed at post-infection 24, 56 and 72 hours using inverted fluorescence microscope. RESULTS AND CONCLUSION: The cell morphology in STEMPRO® MSC SFM was different from other two culture system and these cells were not easy to adherent after trypsin digestion. Cell doubling time in the MesenPRO RS™ Medium was shorter than other two groups. Mesenchymal stem cells were infected by Ad5/35-EGFP with higher efficiency than other two kinds of adenovirus, but part of cells appeared apoptosis. The infection efficiency of Ad5/11-EGFP was highest. The fluorescence intensity was gradually increased with increased MOI.
Shi Y.-Z.,Institute of Hematology |
Shi Y.-Z.,Gene Engineering Co. |
Liu H.-Q.,Gene Engineering Co. |
Jiang L.-L.,Institute of Hematology |
And 10 more authors.
Chinese Pharmacological Bulletin | Year: 2014
Aim: To study targeting capability of anti-CD19 (Fab)-LDM to CD19 + B lymphoma cells in vivo and in vitro. Methods: Flow cytometry was employed to determine the affinity of Cy5 labeled anti-CD19 (Fab)-LDP to human lymphoma Raji cells. And the optical imaging system was used to analyze the distribution of Cy5-anti-CD19 (Fab)-LDP in lymphoma-transplanted xenograft nude mice in vivo. Results: The results of flow cytometry demonstrated that Cy5-anti-CD19(Fab)-LDP had remarkable affinity with lymphoma Raji cells; Raji lymphoma xenograft model was established successfully in nude mice and in vivo fluorescence imaging analysis indicated that the antibody-drug conjugates could specially be localized in the target tumor. Conclusion: The experiments in vivo and vitro confirm that anti-CD19(Fab)-LDP has remarkable affinity to targeting CD19 + lymphoma cells, and the antibody drugs anti-CD19 (Fab)-LDP have the probability to be new drugs for the treatment of malignant lymphoma.