Kim H.J.,Chung - Ang University |
Lee J.Y.,Chung - Ang University |
Kang H.A.,Chung - Ang University |
Lee Y.,GenDocs Inc. |
And 2 more authors.
Letters in Applied Microbiology | Year: 2014
Weak antibody responses to protein antigens after oral immunization remain a serious problem. Yeasts have a rigid cell wall and are inherently resistant to harsh conditions, suggesting that recombinant antigens made in yeast could have a greater chance of making contact with the immune cells of the gastrointestinal (GI) tract in intact form. We compared antibody responses to oral immunization with purified recombinant antigen, used in the conventional manner, and responses to whole recombinant yeast producing the antigen intracellularly. Recombinant capsid protein (CP) of red-spotted grouper necrosis virus (RGNNV) was used as model antigen and Saccharomyces cerevisiae as host. The purified CP was obtained from the S. cerevisiae producing the RGNNV CP. Whole recombinant yeast producing RGNNV CP provoked 9-27 times higher anti-RGNNV CP IgG titres than purified RGNNV CP. Moreover, sera from mice immunized with the recombinant yeast had neutralizing activity against RGNNV, while those from mice immunized with purified CP did not. These results show that whole recombinant yeast is a promising platform for antigen delivery by oral immunization. © 2013 The Society for Applied Microbiology.
Windram O.,University of Warwick |
Windram O.,Imperial College London |
Madhou P.,University of Warwick |
Madhou P.,King's College London |
And 32 more authors.
Plant Cell | Year: 2012
Transcriptional reprogramming forms a major part of a plant's response to pathogen infection. Many individual components and pathways operating during plant defense have been identified, but our knowledge of how these different components interact is still rudimentary. We generated a high-resolution time series of gene expression profiles from a single Arabidopsis thaliana leaf during infection by the necrotrophic fungal pathogen Botrytis cinerea. Approximately one-third of the Arabidopsis genome is differentially expressed during the first 48 h after infection, with the majority of changes in gene expression occurring before significant lesion development. We used computational tools to obtain a detailed chronology of the defense response against B. cinerea, highlighting the times at which signaling and metabolic processes change, and identify transcription factor families operating at different times after infection. Motif enrichment and network inference predicted regulatory interactions, and testing of one such prediction identified a role for TGA3 in defense against necrotrophic pathogens. These data provide an unprecedented level of detail about transcriptional changes during a defense response and are suited to systems biology analyses to generate predictive models of the gene regulatory networks mediating the Arabidopsis response to B. cinerea. © 2012 American Society of Plant Biologists. All rights reserved.
Seo J.Y.,Chonnam National University |
Chung H.J.,GenDocs Inc. |
Kim T.J.,Chonnam National University
Journal of Fish Diseases | Year: 2013
Fish iridovirus causes systemic disease with high morbidity and mortality in various species of wild and farm-raised fish, resulting in severe economic losses. Recently, frequent outbreaks of iridovirus infection have occurred among cultured fish in many Asian countries, emphasizing the need for a protective vaccine programme or the development of a suitable therapy. In this study, we expressed a recombinant major capsid protein (rMCP) of rock bream iridovirus (RBIV) from yeast using codon optimization. The rMCP in yeast was added to feed in an attempt to induce intestinal mucosal immunity for protection against and/or to reduce the severity of fish iridovirus infection. We found that fish immunized orally with rMCP underwent a successful induction of antibodies (P < 0.05) and were protected (P = 0.0001) against viral challenge. Based upon these results, oral administration of immunogenic protein as an antigen can be considered a useful method for implementation of vaccine programmes against iridovirus as well as other marine viral diseases. © 2013 John Wiley & Sons Ltd.
Do T.X.,Hankyong National University |
Lim Y.-I.,Hankyong National University |
Jang S.,GenDocs Inc. |
Chung H.-J.,GenDocs Inc. |
Lee Y.-W.,GenDocs Inc.
Computer Aided Chemical Engineering | Year: 2014
Approximately one million metric ton of empty fruit bunch (EFB), which is a waste of the palm oil industry are discharged every year mainly in Malaysia and Indonesia. It is one of the most recent renewable energy resources and promises high yield in bioethanol productions. The objectives of this study are to conceptually design and economically analyze a whole bioethanol plant using eletrolyzed-reduced water (ERW) as the pretreatment method. To achieve the first objective, a comprehensive model of the bioethanol production plant is developed employing a process simulator on the basis of an experimental study in a pilot plant. The bioethanol production plant consists of five main areas: feed handling, pretreatment & conditioning, saccharification & co-fermentation, product purification, and wastewater treatment. For the second objective, the total capital investment (TCI) is estimated for 100 kton-dry EFB/yr plant. The economic analysis is performed in terms of the payback period (PBP), return on investment (ROI) and the product value (PV). The sensitivity of key variables is analyzed to find the potential reduction of PV. © 2014 Elsevier B.V.
Park J.Y.,GenDocs Inc. |
Lee D.S.,Korea University |
Chung H.-J.,GenDocs Inc.
Plant Biosystems | Year: 2013
Many organisms have developed mechanisms to sense and respond to internal or external soluble sugars for the maintenance of growth and metabolism. In higher plants, the soluble sugars act as important signaling molecules that affect a wide range of biological functions, including flowering time, seed, and early seedling development. Although these sugars act in concert with various cellular components, only few are currently known. Trehalose is present in many prokaryotic and eukaryotic organisms. Its function as an energy source or cellular protectant under stress conditions has been well studied in yeast and Escherichia coli. In plants, however, there is only limited knowledge of the functions of endogenous trehalose and its hydrolytic enzyme trehalase. Therefore, we isolated a T-DNA knockout plant, Attre1, with impaired trehalase activity. The Attre1 mutant contained elevated levels of endogenous trehalose, and exhibited phenotypic abnormalities in both vegetative and reproductive organ development, including growth retardation, abnormal leaf and flower morphologies, and impaired pollen production. Interestingly, a disruption of AtTRE1 resulted in alterations in trehalose synthesis and expression of hexokinase genes. The presented results indicate that Arabidopsis contains a trehalose-signaling network which might be functionally coupled to a hexokinase-dependent signaling pathway, consequently controlling plant metabolism and development. © 2013 Societá Botanica Italiana.
Noh S.A.,Korea University |
Lee H.-S.,Korea Research Institute of Bioscience and Biotechnology |
Kim Y.-S.,GenDocs Inc. |
Paek K.-H.,Korea University |
And 2 more authors.
Journal of Experimental Botany | Year: 2013
The role of an expansin gene (IbEXP1) in the formation of the storage root (SR) was investigated by expression pattern analysis and characterization of IbEXP1-antisense sweetpotato (Ipomoea batatas cv. Yulmi) plants in an attempt to elucidate the molecular mechanism underlying SR development in sweetpotato. The transcript level of IbEXP1 was high in the fibrous root (FR) and petiole at the FR stage, but decreased significantly at the young storage root (YSR) stage. IbEXP1-antisense plants cultured in vitro produced FRs which were both thicker and shorter than those of wild-type (WT) plants. Elongation growth of the epidermal cells was significantly reduced, and metaxylem and cambium cell proliferation was markedly enhanced in the FRs of IbEXP1-antisense plants, resulting in an earlier thickening growth in these plants relative to WT plants. There was a marked reduction in the lignification of the central stele of the FRs of the IbEXP1-antisense plants, suggesting that the FRs of the mutant plants possessed a higher potential than those of WT plants to develop into SRs. IbEXP1-antisense plants cultured in soil produced a larger number of SRs and, consequently, total SR weight per IbEXP1-antisense plant was greater than that per WT plant. These results demonstrate that SR development was accelerated in IbEXP1-antisense plants and suggest that IbEXP1 plays a negative role in the formation of SR by suppressing the proliferation of metaxylem and cambium cells to inhibit the initial thickening growth of SRs. IbEXP1 is the first sweetpotato gene whose role in SR development has been directly identified in soil-grown transgenic sweetpotato plants. © 2012 The Author. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.
GenDocs Inc. | Date: 2010-03-31
The present invention relates to environmental stress-inducible 972 promoter isolated from rice, a recombinant plant expression vector comprising said promoter, a method of producing a target protein by using said recombinant plant expression vector, a method of producing a transgenic plant using said recombinant plant expression vector, a transgenic plant produced by said method, a method of improving resistance of a plant to environmental stress by using said promoter, and a primer set for amplification of said promoter.
GenDocs Inc. | Date: 2010-07-26
The present invention relates to environmental stress-inducible 557 promoter isolated from rice, a recombinant plant expression vector comprising said promoter, a method of producing a target protein by using said recombinant plant expression vector, a method of producing a transgenic plant using said recombinant plant expression vector, a transgenic plant produced by said method, a method of improving resistance of a plant to environmental stress by using said promoter, and a primer set for amplification of said promoter.