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Bora A.,Gauhati University | Hazarika N.K.,Gauhati Medical College and Hospital | Shukla S.K.,Sanjay Gandhi Post Graduate Institute of Medical Sciences | Prasad K.N.,Sanjay Gandhi Post Graduate Institute of Medical Sciences | And 2 more authors.
Indian Journal of Pathology and Microbiology | Year: 2014

Aim: This study was carried out to determine the presence of bla TEM , blaSHV and blaCTX-M genes in extended-spectrum β-lactamase (ESBL) producing Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) at a tertiary care referral hospital in Northeast India. Materials and Methods: A total of 270 E. coli and 219 K. pneumoniae isolates were recovered during the period between August 2009 and July 2010. Kirby-Bauer disk diffusion method was performed to determine the antibiotic resistance pattern. Screening and phenotypic confirmatory test for ESBL production were performed using standard disc diffusion methods. Each of the initial ESBL screening test isolate was investigated for the presence of blaTEM , blaSHV and blaCTX-M genes via polymerase chain reaction (PCR) using gene-specific primers. Results: Phenotypic confirmatory test able to detect ESBL production in 73.58% of E. coli and 67.24% of K. pneumoniae. However, PCR amplification showed the presence of one or more ESBL genes in each of the initial ESBL screening positive isolate. Among three ESBL genotypes, the most prevalent genotype was found to be bla CTX-M in E. coli (88.67%) and blaTEM in K. pneumoniae (77.58%) ESBL producing isolates. Majority of ESBL producing isolates possess more than one ESBL genes. Conclusion: This study constituted a primer report on high prevalence of blaTEM and blaCTX-M genes in ESBL producing isolates of E. coli and K. pneumoniae and denotes the need of more extensive studies on these antibiotic genes to determine the magnitude of the problem of antibiotic resistance exiting in this locality.

Mazumder A.,Gauhati Medical College and Hospital
Journal of Indian Academy of Forensic Medicine | Year: 2014

Pregnancy is the condition of having a developing embryo or foetus in the female, when an ovum is fertilized by spermatozoa. In twin pregnancy there are two developing foetus in the uterus. The present case is a 29 years old lady with 33 weeks twin pregnancy, who died suddenly and brought for medico-legal autopsy. On examination all the organs were found pale. Heart was found enlarged. Left lung was found to be adherent to the under surface of lungs and chest wall. Post-mortem hypostasis was faint and not fixed. Heart, lungs and liver has been send for Histo-pathological examination. Opinion regarding cause of death was kept pending till the receipt of histopathology report. This is a case of sudden death in advanced pregnancy coming to the mortuary of Forensic Medicine, Gauhati Medical College and Hospital. Such cases emerging as a major burden now days and needs thorough discussion. Hence this case was discussed in details.

Thakur B.K.,Sikkim Manipal Institute of Medical science | Verma S.,Sikkim Manipal Institute of Medical science | Hazarika D.,Gauhati Medical College and Hospital
Indian Journal of Dermatology | Year: 2012

Background: Cutaneous tuberculosis forms a small subset of extra pulmonary tuberculosis and has a worldwide distribution. Aims: The present study is an attempt to find out the incidence, clinical spectrum, and histopathological features of cutaneous tuberculosis. Materials and Methods: A total of 42 cases of newly diagnosed patients of cutaneous tuberculosis attending dermatology out patient department over a period of 1 year were included in the study. A detailed clinical examination and investigations including histopathological examination were carried out. Results: Scrofuloderma was the most common form seen in 50% cases followed by lupus vulgaris in 42.86%, tuberculosis verrucosa cutis in 4.76%, and lichen scrofulosorum in 2.38% cases. The Mantoux test was positive in 83.33% cases. Characteristic tuberculoid granulomas were seen in 72.22% cases of lupus vulgaris, 42.86% cases of scrofuloderma and all cases of tuberculosis verrucosa cutis and lichen scrofulosorum. Conclusion: Cutaneous tuberculosis is still highly prevalent in upper Assam. Early diagnosis and treatment are essential to prevent its complications.

Jyoti Nath S.,Gauhati Medical College and Hospital
UroToday International Journal | Year: 2013

Introduction: Urolithiasis typically affects adult men more commonly than women with a two- to threefold higher incidence. Stone disease has been found to have an inverse relationship as one ages. This gender and age differentiation invites the role of testosterone as a possible lithogenic factor. Our study aims at determining the role of testosterone as lithogenic factor and its relation to urinary and stone components. Methods: A total of 87 men older than 18 years were enrolled in our study. Early morning free and total testosterone, serum level of the parathyroid hormone, calcium acid, and uric acid were estimated between the 2 groups. Twenty-four hours of urinary oxalate, uric acid, and calcium were estimated in all patients and the control. Stone analysis was done to determine stone composition for stones in the former group. Results: Of the 87 patients, 48 patients had a history of urolithiasis and 39 patients had no history of urolithiasis. In comparison to non-stone formers, stone formers have higher serum-free and total testosterone, and 24-hour urinary oxalate. A positive and weak-positive correlation was found in relation to 24 hours of urinary oxalate for free and total testosterone, respectively. Calcium oxalate stones were found in 40 patients. Other differences between the 2 groups were not statistically significant. Conclusions: A higher serum-free and total testosterone, and 24 hours of urinary oxalate were found in male stone formers compared to non-stone formers. Serum testosterone had a positive correlation to urinary oxalate. These findings have a similarity with animal and human studies. A larger prospective study is necessary to establish testosterone as a lithogenic factor in male former-stone patients. © 2013 Digital Science Press, Inc.

Mahanta P.,Gauhati Medical College and Hospital
International Journal of Medical Toxicology and Legal Medicine | Year: 2010

This presentation describes 100 deaths with abdomino-pelvic injuries. Most vulnerable age group was (21-30) years with male female ratio 9:1. Incidence of lower socioeconomic, under graduate, bachelor and businessmen group was more. Urban incidents were more with 43% that to in between 09.01pm to 12.00 am. A heavy truck was the most common offending vehicle with pedestrian as common category. Spleen was the most common (52%) organ injured. Peritoneal haemorrhages (54%) were the common accompanying finding. Injuries to two or more than two body region were present in 27% of cases. The present study also described four burn cases. Maximum (34%) cases died on spot. Person of age group 21-30 years and 31-40 years had long survival period as compared to the children up to 10 years of age and elderly people more than 60 years. Shock and haemorrhage was the main cause of death for 55% of cases. Seat belt and crash helmets were found important besides other like mass people education in preventing fatal injuries. Development of infrastructure of road agent and easy availability of multi disciplinary medical staff as and where required is also equally important.

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