Kurt Y.G.,GATA Tibbi Biyokimya AD |
Cayci T.,GATA Tibbi Biyokimya AD |
Akgul E.O.,GATA Tibbi Biyokimya AD |
Aydn I.,GATA Tibbi Biyokimya AD |
And 7 more authors.
Turkish Journal of Biochemistry | Year: 2010
Purpose: The hemoglobin A1c test is widely used in monitoring the treatment of diabetes mellitus patients. In this study, it is aimed to investigate the effect of variant hemoglobins on hemoglobin A1c measurements by evaluating hemoglobin A1c values of patients with the results of hemoglobin electrophoresis. Materials and Methods: The results of 60 patients which had been studied both hemoglobin A1c and hemoglobin electrophoresis was analyzed retrospectively. Hemoglobin A1c measurements were performed with high pressure liquid chromatography system with UV-Vis detector by using two different programs (standard and corrected). Results: Average hemoglobin A1c level of 60 patients measured by standart program was 6.5% ± 2.3 (mean ± standard deviation). Hemoglobin A1c results of 22 patients (45%) were above 6.5%. The mean hemoglobin A1c level measured by the corrected program was 5.5% ± 1.5 (p<0.001). With the corrected program, the number of patients that had hemoglobin A1c results over 6.5% was 12 (20%). In 33 of 60 patients (55%), hemoglobin electrophoresis results were pathologic. Conclusion: Unnecessary and wrong treatments could be given to patients depending on the wrong high hemoglobin A1c results, measured by high pressure liquid chromatography. Therefore to minimize morbidity and mortality due to incorrect hemoglobin A1c measurements, to consider the interference due to hemoglobin variant is of great importance in terms of laboratories. © TurkJBiochem.com.