Gansu Provincial Tumor Hospital

Lanzhou, China

Gansu Provincial Tumor Hospital

Lanzhou, China
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Kamarajugadda S.,University of Florida | Cai Q.,University of Florida | Chen H.,University of Florida | Nayak S.,Florida College | And 8 more authors.
Cell Death and Disease | Year: 2013

Normal cells require adhesion to extracellular matrix for survival. Cell detachment causes a drastic increase in reactive oxygen species (ROS) that promotes anoikis. In the present study, we observed that upon detachment from matrix, human mammary epithelial cells strongly upregulate manganese superoxide dismutase (MnSOD, or SOD2), a principal mitochondrial antioxidant enzyme that detoxifies ROS through dismutation of superoxide. Induction of MnSOD by cell detachment is dependent on the NFκB transcription factor. Detachment of mammary epithelial cells potently increases mitochondrial superoxide levels, which are further elevated by depletion of MnSOD in suspended cells. Consequently, cells depleted of MnSOD are hypersensitive to matrix detachment and exhibit increased anoikis. These results suggest that detachment-induced MnSOD counters mitochondrial superoxide accumulation and confers anoikis resistance. Taken together with our previous finding that detached cells evade excessive ROS production by attenuating oxidative metabolism of glucose, we conclude that mammary epithelial cells coordinate their responses to detachment through increasing MnSOD and decreasing ROS generation from mitochondrial glucose oxidation, thereby mitigating anoikis. Anoikis is a barrier to tumor metastasis. Indeed, MnSOD expression is elevated in human breast cancer metastases compared with primary tumors. Expression of MnSOD correlates with histologic tumor grades in human cancer and contributes to cancer cell's resistance to anoikis. Our study suggests that inhibition of ROS detoxification coupled with stimulation of glucose oxidative metabolism may be an efficient strategy to enhance anoikis and block metastasis. © 2013 Macmillan Publishers Limited All rights reserved.


Su H.-X.,Institute for Medical Research | Su H.-X.,Gansu Provincial Tumor Hospital | Zhou H.-H.,Institute for Medical Research | Zhou H.-H.,Gansu Provincial Tumor Hospital | And 15 more authors.
PLoS ONE | Year: 2014

C-reactive protein (CRP) is an established marker of inflammation with pattern-recognition receptor-like activities. Despite the close association of the serum level of CRP with the risk and prognosis of several types of cancer, it remains elusive whether CRP contributes directly to tumorigenesis or just represents a bystander marker. We have recently identified recurrent mutations at the SNP position -286 (rs3091244) in the promoter of CRP gene in several tumor types, instead suggesting that locally produced CRP is a potential driver of tumorigenesis. However, it is unknown whether the -286 site is the sole SNP position of CRP gene targeted for mutation and whether there is any association between CRP SNP mutations and other frequently mutated genes in tumors. Herein, we have examined the genotypes of three common CRP non-coding SNPs (rs7553007, rs1205, rs3093077) in tumor/normal sample pairs of 5 cancer types (n = 141). No recurrent somatic mutations are found at these SNP positions, indicating that the -286 SNP mutations are preferentially selected during the development of cancer. Further analysis reveals that the -286 SNP mutations of CRP tend to co-occur with mutated APC particularly in rectal cancer (p = 0.04; n = 67). By contrast, mutations of CRP and p53 or K-ras appear to be unrelated. There results thus underscore the functional importance of the -286 mutation of CRP in tumorigenesis and imply an interaction between CRP and Wnt signaling pathway. © 2014 Su et al.


Song J.,Gansu Provincial Tumor Hospital | Li J.,Gansu Provincial Tumor Hospital | Han F.,Gansu Provincial Tumor Hospital | Fu L.,Gansu Provincial Tumor Hospital
Chinese Journal of Clinical Oncology | Year: 2010

Objective: To assess the effect of simplifying surgical processes, shortening the period of perfusion and increasing surgical safety for the improved hyperthermic isolated limb perfusion (HILP) technique. Methods: Ninety-seven patients with malignant tumor (71 with malignant melanoma, 18 with soft tissue sarcoma and 8 with bone sarcoma) of an extremity underwent HILP. Sixteen patients were treated with standard HILP and 81 patients were treated with improved HILP. This method included changing the way for inserting cannulas, adding a drug for blood vessel dilatation in the perfusate and measuring the temperature of limb muscles. Results: Fifty-four patients were followed-up for 6 months to 8 years. All tumors became small and soft after HILP. Tumor resection was performed at 15 to 25 days after perfusion. Complete histopathological response of tumor was evident in 23 cases, partial histopathological response was evident in 52 cases and no histopathological response was evident in 22 cases. In the group that received improved HILP, no fascial compartment syndrome occurred (3 cases in the standard HILP group), the perfusion speed was increased, and limb swelling was moderate. Conclusion: Inserting cannulas into the femoral vein or axillary vein from the amputation stump of the long saphenous vein or cephalic vein made the procedure easier and there was no obstruction of blood or backflow that occurred. Expanding the vessel of the limb being perfused can increase the infusion speed to achieve hyperthermia (41°C-42°C) earlier. Measuring the temperature of limb muscles and maintaining a temperature below 43°C may prevent the occurrence of fascial compartment syndrome.


Shi H.-P.,Lanzhou University | Zhang Q.-N.,Gansu Provincial Tumor Hospital | Liu G.-Q.,Lanzhou University | Wang X.-H.,Lanzhou University | Wang X.-H.,Gansu Provincial Tumor Hospital
Journal of Practical Oncology | Year: 2014

Objective: To evaluate the clinical efficacy and safety of hyperthermia (HT) combined with pleural infusion chemotherapy(observation group) vs pleural infusion chemotherapy alone(control group) for malignant pleural effusion. Methods: Randomized controlled trials (RCTs) on HT plus pleural infusion chemotherapy vs pleural infusion chemotherapy alone for malignant pleural effusion were searched in PubMed, Cochrane Library, EMBASE, VIP, Wanfang, CNKI, CBM, Chinese Journal Full-text Database and other electronic databases. Two reviewers independently screened studies according to the inclusion and exclusion criteria, extracted data and assessed quality of the included studies independently, based on the cochrane system evaluation method. Then meta-analyses were performed using RevMan 5.1. Results: A total of 19 RCTs involving 1 134 cases were included. Meta-analysis results showed that the efficacy rate(OR=3.82, 95%CI: 2.89~5.06;P<0.00001) and life quality improvement rate (OR=3.34, 95%CI: 2.39~4.68;P<0.00001) of observation group were higher than those of control group. In the safety aspect, there were no significant differences in the incidence of gastrointestinal reaction, myelosuppression and chest pain between the two groups; but the incidence rates of skin burn(OR=4.88, 95%CI: 1.38~17.22, P=0.01) and subcutaneous scleroma(OR=5.41, 95%CI: 1.36~21.47, P=0.02) in observation group were higher than those in control group. Conclusion: Compared with pleural infusion chemotherapy alone, hyperthermia combined with pleural infusion chemotherapy can significantly improve the efficacy and life quality of patients with malignant pleural effusion, and its side effects are acceptable.


Li J.,Gansu Provincial Tumor Hospital | Wang P.,Gansu Provincial Tumor Hospital | Zhu X.-J.,Gansu Provincial Tumor Hospital | Song J.-G.,Gansu Provincial Tumor Hospital | Song J.-M.,Gansu Provincial Tumor Hospital
Chinese Journal of Oncology | Year: 2012

Objective To investigate the effect of isolated limb hyperthermic perfusion chemotherapy for melanoma of the extremities. Methods Limb isolated hyperthermic perfusion chemotherapy was performed in 41 patients with malignant melanoma of the extremities, and then the primary lesions in 24 patients were removed at 14-21 days after chemotherapy. Tumor necrosis was examined by pathology. Results Among the 41 patients, 40 cases were followed up for 6-113 months, and one was lost. There was no local recurrence in those patients. 29 cases were followed up for more than 3 years, and 26 of them were surviving. Forteen cases were followed up for more than five years, among them 9 cases were surviving. The 3-year and 5-year survival rates of the whole group were 95.0% and 70.0%, respectively. The average reduction of the tumor volume was 55.6% after perfusion. The pathological examination showed that tumor necrosis was 90%-100% (complete response) in 21 cases (87.5%) and 60%-89% (partial response) in 3 cases (12.5%). Conclusions The isolated limb hyperthermic perfusion chemotherapy is an effective treatment of limb malignant melanoma. It can significantly reduce the local recurrence rate, and improve the 5-year survival rale, prognosis and the quality of life of the patients.


Luo H.-T.,Gansu Provincial Tumor Hospital | Wang X.-H.,Gansu Provincial Tumor Hospital | Wei S.-H.,Gansu Provincial Tumor Hospital | Tian J.-H.,Lanzhou University | Yang K.-H.,Lanzhou University
Chinese Journal of Cancer Prevention and Treatment | Year: 2010

OBJECTIVE: To evaluate the efficiency and safety of external beam radiotherapy plus brachytherapy ( EBR + B) compared with external beam radiotherapy alone in the treatment of esophageal cancer. METHODS: CBMdisc, VIP, CNKI, Cochrane Library, Pubmed, Embeas etc. were searched by computer and eight major Chinese journals of oncology were searched by manual. Randomized and quasi-randomized controlled trials compared external beam radiotherapy plus brachytherapy with external beam radiotherapy alone in the treatment of esophageal cancer were included. The statistical software RevMan 4. 2. 10 was used. RESULTS: Fourteen published articals were included (1400 patients), and all trails methodological quality were grade C. The results of Meta-analysis showed that: 1,3-year survival rates of EBR+B was significantly higher than that in EBR alone, while the tumor regrowth rates reduced, and the difference was significant (P values were 0. 03, <0. 000 01, 0. 005 and 0. 02 respectively), but the 5-year survival rates and tumor metastasis rates in the two groups were not significant different (P values were 0. 29 and 0. 43 respectively). As for the occurrence rates of radiation esophagitis, bleeding, benign esophageal stenosis in EBR+ B were higher than those to EBR, and there were significant difierent (P value were 0. 04, 0. 002 and 0. 03 respectively), while esophageal perforation and esophageal ulcer were not significantly different between the two groups (P values were 0. 77 and 0. 20 respectively). Radiation pneumonitis in EBR was higher than that in EBR+B with a significant difference (P = O. 000 5). CONCLUSIONS: Compared with EBR alone, EBR+B can improve shortterm survival rates and reduce the toumor regrowth, but not improve longterm survival rates and reduce metastasis rates. There are more incidences of adverse reactions in EBR+B.


Wan Q.,Gansu Provincial Tumor Hospital | Xi A.,Gansu Academy of Medical Science | Zhang C.,Gansu Provincial Tumor Hospital | Liu X.,Gansu Provincial Tumor Hospital
Zhongguo Zhongyao Zazhi | Year: 2011

Objective: To assess the efficacy and toxicity of the kangai injection combination of fludarabine(Flud), cytosine arabinoside (Ara-C), and granulocyte colony-stimulating factor (G-CSF) (FLAG) in refractory/relapsed acute leukemia (AL) patients. Method: From 2004 to 2010 in our hospital, the 49 cases of refractory/relapsed acute luekemia were randomly divided into treatment group(28 cases) and control group(21 cases). The control group were treated by kangai injection plus FLAG regimen,and the control group were treated by FLAG regimen. Result: The remission rate of treatment and total effective rate treatment group were 57.1%(16/28) and 71.4% (21/28), the control group were 52.3% (11/21) and 61.9% (13/21), there were no significant differences in the two groups. Duration of neutrophils less than 0.5 × 10 9/L in treatment group was (14±6) day, control group was (23 ± 3) day, Duration of platelet less than 25 ± 10 9/L in treatment group was (17 ± 6) day, control group was (31 ± 2) day, treatment group of III-IV degree of infection was 6.9% (1/28) and control group was 23.8% (5/21) between the two groups were significantly different (P < 0.05). treatment group of III-IV degree of gastrointestinal toxicity was 10.7% (3/28) arid control group was 28.5% (6/21). Conclusion: Kangai injection plus FLAG regimen could increase the remission rate, shorten the period of bone marrow suppression, significantly reduced the incidence and degree of infection, play a important role in attenuated efficiency.


Zhang Z.-G.,Lanzhou University | Zhang Q.-N.,Lanzhou University | Zhang Q.-N.,Gansu Provincial Tumor Hospital | Wang X.-H.,Lanzhou University | And 2 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2013

Background and Objective: Though researched for years, the prognostic role of hypoxia-inducible factor 1 alpha (HIF-1α) in gastric cancer is still controversial. We thus undertook a systematic review to assess the relationship. Method: A systematically literature search of Pubmed, Embase, Web of Science, China Biological Medicine Disc and Cochrane Library was undertaken in February 2013, and the reference lists of articles were retrieved. Results: 12 trials (1,555 participants) were included to assess the association between HIF-1α expression and survival. Summary hazard ratios (HRs) were calculated. HIF-1α expression was significantly correlated with poor overall survival of gastric cancer patients (HR=1.34, 95%CI: 1.13-1.58; P=0.0009), but not with poor disease free survival of gastric cancer patients (HR=1.67, 95%CI: 0.99-2.82; P=0.06). Conclusion: HIF-1α was associated with poor OS, but not DFS, especially for Asian patients. But studies evaluating relationships of HIF-1α with OS and DFS in non-Asian gastric cancer patients appear needed.


PubMed | Gansu Provincial Tumor Hospital
Type: Journal Article | Journal: Zhonghua zhong liu za zhi [Chinese journal of oncology] | Year: 2013

To investigate the effect of isolated limb hyperthermic perfusion chemotherapy for melanoma of the extremities.Limb isolated hyperthermic perfusion chemotherapy was performed in 41 patients with malignant melanoma of the extremities, and then the primary lesions in 24 patients were removed at 14 - 21 days after chemotherapy. Tumor necrosis was examined by pathology.Among the 41 patients, 40 cases were followed up for 6-113 months, and one was lost. There was no local recurrence in those patients. 29 cases were followed up for more than 3 years, and 26 of them were surviving. Forteen cases were followed up for more than five years, among them 9 cases were surviving. The 3-year and 5-year survival rates of the whole group were 95.0% and 70.0%, respectively. The average reduction of the tumor volume was 55.6% after perfusion. The pathological examination showed that tumor necrosis was 90% - 100% (complete response) in 21 cases (87.5%) and 60% - 89% (partial response) in 3 cases (12.5%).The isolated limb hyperthermic perfusion chemotherapy is an effective treatment of limb malignant melanoma. It can significantly reduce the local recurrence rate, and improve the 5-year survival rate, prognosis and the quality of life of the patients.


PubMed | Gansu Provincial Tumor Hospital
Type: Journal Article | Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica | Year: 2012

To assess the efficacy and toxicity of the kangai injection combination of fludarabine (Flud), cytosine arabinoside (Ara-C), and granulocyte colony-stimulating factor (G-CSF) (FLAG) in refractory/relapsed acute leukemia (AL) patients.From 2004 to 2010 in our hospital, the 49 cases of refractory/relapsed acute luekemia were randomly divided into treatment group (28 cases) and control group (21 cases). The control group were treated by kangai injection plus FLAG regimen, and the control group were treated by FLAG regimen.The remission rate of treatment and total effective rate treatment group were 57.1% (16/28) and 71.4% (21/28), the control group were 52.3% (11/21) and 61.9% (13/21), there were no significant differences in the two groups. Duration of neutrophils less than 0.5 x 10(9)/L in treatment group was (14 +/- 6) day, control group was (23 +/- 3) day, Duration of platelet less than 25 x 10(9)/L in treatment group was (17 +/- 6) day, control group was (31 +/- 2) day, treatment group of III-IV degree of infection was 6.9% (1/28) and control group was 23.8% (5/21) between the two groups were significantly different (P < 0.05). treatment group of III- IV degree of gastrointestinal; toxicity was 10.7% (3/28) and control group was 28. 5% (6/ 21).Kangai injection plus FLAG regimen could increase the remission rate, shorten the period of bone marrow suppression, significantly reduced the incidence and degree of infection, play a important role in attenuated efficiency.

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