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Yao Y.,Lanzhou University | Cui H.,Gansu Provincial Maternity and Child Care Hospital | Liu Y.,China Mobile Communications Group | Li L.,Lanzhou University | And 2 more authors.
Mathematical Problems in Engineering | Year: 2015

We propose an optimized Support Vector Machine classifier, named PMSVM, in which System Normalization, PCA, and Multilevel Grid Search methods are comprehensively considered for data preprocessing and parameters optimization, respectively. The main goals of this study are to improve the classification efficiency and accuracy of SVM. Sensitivity, Specificity, Precision, and ROC curve, and so forth, are adopted to appraise the performances of PMSVM. Experimental results show that PMSVM has relatively better accuracy and remarkable higher efficiency compared with traditional SVM algorithms. © 2015 Yukai Yao et al. Source


Liu J.,Peoples Hospital of Gansu Province | Cao L.,Gansu Provincial Maternity and Child Care Hospital | Liu L.,Peoples Hospital of Gansu Province | Guo S.,Peoples Hospital of Gansu Province | And 2 more authors.
Tumor Biology | Year: 2014

Hemangiopericytoma (HPC) is an uncommon highly vascular neoplasm that originated from Zimmerman’s pericytes which surrounds the endothelial tissue. Primary epidural HPC of the sacrum is extremely rare. We reported an unusual case of primary epidural malignant HPC of the sacrum that invaded vertebral bone and caused rectum compression in a 57-year-old male for the first time. The patient presented progressive low back pain and ribbon-like stool over 3 months. The surgical intervention involved sacrectomy and en bloc resection of the tumor. We described the clinical, radiological, and histological features of this tumor and reviewed the literature. © 2014, International Society of Oncology and BioMarkers (ISOBM). Source


Li X.,Lanzhou University | Wang Z.-X.,Gansu Provincial Maternity and Child Care Hospital | Wang Z.-S.,Lanzhou University | Li Q.-F.,Lanzhou University
Asian Pacific Journal of Tropical Medicine | Year: 2015

Objective: To explore the effect of microRNA-101 on apoptosis of condylar cartilage cells and the specific mechanism of molecular biology. Methods: IL-1 was used to stimulate and establish the model of apoptosis of condylar cartilage cells. The expression change of miR-101 in control group was compared with that in IL-1 stimulation group by qRT-PCR. Overexpression and down-regulation models of miR-101 were established by transfecting Mimics and Inhibitor and verified by qRT-PCR. Flow cytometry was used to detect the effect of miR-101 overexpression and down-regulation on apoptosis. Target gene of miR-101 was analyzed and calculated through bioinformatics. Western blot and Luciferase report assay were used to detect whether SOX9 could become the target gene of miR-101. Results: qRT-PCR results showed that IL-1 stimulation could cause the increase of miR-101 expression. After the transfection of rabbit condylar cartilage cells by Mimics and Inhibitor, qRT-PCR results confirmed the significant effect of miR-101 overexpression and down-regulation. It was confirmed by flow cytometry that overexpression of miR-101 could promote the apoptosis of condylar cartilage cells, and down-regulation of miR-101 could reduce the apoptosis. It was confirmed by Western blot and Luciferase report assay that SOX9 was the target gene of miR-101, and miR-101 inhibited SOX9 expression through complementary pairing with 3'UTR of SOX9 mRNA. Conclusions: miR-101 can promote the apoptosis of condylar cartilage cells through inhibiting the protein level of target gene SOX9. © 2015 Hainan Medical College. Source


Jin Y.,Nanjing University | Zhang R.-F.,Gansu Provincial Maternity and Child Care Hospital | Xie Z.-P.,Chinese National Institute for Viral Disease Control and Prevention | Yan K.-L.,Nanjing Childrens Hospital | And 5 more authors.
Virology Journal | Year: 2013

Background: Human adenovirus (HAdV) is an important agent causing respiratory tract infection in children. Information on the epidemiological and clinical features of HAdV is limited in children with acute respiratory tract infections (ARTIs) in China, especially those of a novel genotype, Ad55. Methods. In total, 1169 nasopharyngeal aspirates were collected from children younger than 14 years with ARTIs between November 2006 and November 2009. The polymerase chain reaction (PCR) was used to screen HAdVs. All PCR-positive products were sequenced. Results: 74 of 1169 (6.33%) specimens were positive for HAdVs. Among positive cases, AdV3 (58/74) was detected most frequently, followed by AdV11 (10/74), AdV2 (2/74), AdV7 (2/69), AdV6 (1/74), and AdV1 (1/74). AdV55 was found in one case. The incidence of HAdV infection peaked in children aged 3-7 years. The most common clinical diagnosis was upper respiratory infection, and the most common syndrome was fever and cough.The comparison of HAdV and RSV group revealed that Children infected with group AdV were significant older than children infected with group RSV, had more fever but less frequently wheezing, and cough, crackles, and cyanosis, The duration of hospitalization between the AdV group and RSV group was not significant, but a greater frequency of LRTIs was observed in RSV group. Conclusions: HAdV is an important viral agent in children with ARTIs in Lanzhou City, China. Multiple HAdV serotypes co-circulated with Ad3, which was predominant in this 3-year study. The novel AdV55 genotype was found in one case. No fixed seasonal rhythm could be identified. © 2013 Jin et al.; licensee BioMed Central Ltd. Source


Lv Y.,Lanzhou Jiaotong University | Xue X.,Gansu Provincial Maternity and Child Care Hospital | Tao L.,Lanzhou Jiaotong University | Zhang D.,Wuhan University of Technology | And 2 more authors.
Bulletin of Environmental Contamination and Toxicology | Year: 2016

In this study, extracellular polymeric substances of Aphanizomenon flos-aquae (EPS-A) were investigated in order to explore their effect on astrocytes of zebrafish and potential risk for environment. Astrocytes were treated with varying concentrations of EPS-A, the results showed that EPS-A inhibited astrocytes growth in a dose-and time-dependent manner. With the concentrations of EPS-A increasing, the adherent ability of astrocytes decreased and the number of astrocytes floating in the culture medium increased. When treated with 2.35 µg/mL EPS-A, EPS-A induced cell cycle arrest and made the collapse of mitochondrial membrane potential and then led to astrocytes apoptosis. The results suggested that EPS-A could pose a threat to zebrafish and represent risk for environment, so regularly monitoring the presence of EPS-A was very important in nutrient-rich freshwaters when A. flos-aquae blooms broke out. © 2016 Springer Science+Business Media New York Source

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