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Liu Y.,CAS Lanzhou Institute of Chemical Physics | Liu Y.,University of Chinese Academy of Sciences | Sun X.,CAS Lanzhou Institute of Chemical Physics | Sun X.,University of Chinese Academy of Sciences | And 4 more authors.
Clinica Chimica Acta | Year: 2011

Background: Uric acid (UA) is the only biomedical indicator for gout in clinic that always leads to an uncertain diagnose. Due to the lack of reliable metabolites, it is now already highly desirable to diagnose gout definitely. Methods: Metabonomics was employed to screen and identify novel biomarkers of gout based on human serum and urine. High performance liquid chromatography-diode array detector (HPLC-DAD) and orthogonal signal correction partial least squares discriminate analysis (OSC-PLS-DA) were also used for metabonomics study. Results: Several potential biomarkers including uric acid, creatinine, tryptophan in serum and uric acid, creatinine, guanosine, hippuric acid in urine, were respectively screened and identified. For serum and urine, the predictive levels about the OSC-PLS-DA models of the gout and controls were 95.76% and 100%, and the correction levels about the seriousness of the disease were 90.32% and 87.5%, respectively. Conclusion: Compared with intermittent gout, the acute gout shows clearly the dysfunctions of purine, protein and glucose metabolism. The metabolizing of guanosine to UA increases the levels of UA in serum at the acute stage. Our research contributes to a better understanding of the metabolic mechanism and allowing the targeted therapy of gout at different stages. © 2011 Elsevier B.V. Source


Xia J.-F.,Lanzhou University | Xia J.-F.,CAS Lanzhou Institute of Modern Physics | Wang Z.-Z.,CAS Lanzhou Institute of Modern Physics | Liu Q.-F.,CAS Lanzhou Institute of Modern Physics | And 3 more authors.
Nuclear Science and Techniques | Year: 2014

To study cytotoxic effect of heavy ion irradiation in the plateau region, and investigate whether autophagy induced by heavy ion irradiation is cytoprotective, HeLa cells were irradiated with 350 MeV/u carbon ions beams, and the clonogenic survival was analyzed. The results showed that cell survival decreased with increasing doses. It was also found that G2/M-phase cells increased, and the autophagy-related activity was significantly higher than the control. When autophagy was blocked by 3-methyladenine in carbon-ion irradiated cells, G2/M phase arrest and the percentage of apoptosis cells were further elevated, and cell survival decreased significantly, indicating the induction of cytoprotective autophagy by carbon-ion irradiation. Our results demonstrated that autophagy induced by carbon ion irradiation provided a self-protective mechanism in HeLa cells, short-time inhibition of autophagy before carbon-ion irradiation could enhance radiation cytotoxicity in HeLa cells. © 2014, Science Press. All rights reserved. Source


Fangming G.,Tianjin Medical University | Xiaohuan W.,Gansu Province People Hospital | Guangping L.,Tianjin Medical University | Xin C.,Tianjin Medical University | Juexin F.,Yantai Hill Hospital and China France Friendship Hospital
Journal of Medical Colleges of PLA | Year: 2010

Objective: The aim of the present study was to assess the early clinical outcome and risk factors in old patients with acute ST elevation myocardial infarction (STEMI) following primary percutaneous coronary intervention (PCI). Methods: A total of 136 patients older than 60 years with STEMI who received successful PCI were included in this study. The patients were classified in 2 age groups: patients =75 years and <75 years of age. The extent of coronary artery lesions was measured by quantitative coronary artery angiography (QCA). Subjects were tracked for subsequent cardiovascular events: cardiac death, myocardial infarction, heart failure, percutaneous coronary intervention, coronary artery bypass and stroke. Results: Though the older group had a higher prevalence of adverse baseline characteristics and lower final TIMI flow than patients<75y (P<0.05), the procedural success did not make difference between the two groups. In 12 months follow-up of 136 study participants, there occurred 39 CV events: cardiac death (five patients), heart failure (nineteen patients), and stroke (six patients). Three patients received coronary bypass grafts and six patients underwent PCI. Heart failure and overall cardiovascular event rates were higher in older patients compared with those in patients<75y. The main adverse clinical events (MACE) for the old group were a little higher comparing with the younger in 12-month follow-up (P=0.029 6 and P=0.043 4). Multivariate cox analysis identified that a diagnosis of diabetes (HR 2.495, 95%CI 1.224 to 5.083, P= 0.011 8) and time from symptom(HR 1.450, 95%CI 1.143 to 1.841, P= 0.008 2) to PCI as independent predictors of CV events after adjustment of all entered baseline variables. Conclusion: Our study suggests that drug-eluting stent implantation in older patients with acute ST elevation myocardial infarction has high initial procedural success rates despite having more severe baseline risk characteristics, and to shorten the time from symptom onset to PCI may decrease cardiovascular events in old patients following PCI. © 2010 The Editorial Board of Journal of Medical Colleges of PLA. Source


Hou M.,Lanzhou University | Xie J.-F.,Lanzhou University | Kong X.-P.,Lanzhou University | Kong X.-P.,Hunan Normal University | And 7 more authors.
Toxins | Year: 2015

Onabotulinumtoxin A (BoNTA) has been reported to be effective in the therapy for migraines. Acupuncture has been used worldwide for the treatment of migraine attacks. Injection of a small amount of drug at acupuncture points is an innovation as compared to traditional acupuncture. The purpose of this study was to evaluate and compare the effectiveness of fixed (muscle)-site and acupoint-site injections of BoNTA for migraine therapy in a randomized, double-blinded, placebo-controlled clinical trial extending over four months. Subjects with both episodic and chronic migraines respectively received a placebo (n = 19) or BoNTA (2.5 U each site, 25 U per subject) injection at fixed-sites (n = 41) including occipitofrontalis, corrugator supercilii, temporalis and trapeziue, or at acupoint-sites (n = 42) including Yintang (EX-HN3), Taiyang (EX-HN5), Baihui (GV20), Shuaigu (GB8), Fengchi (GB20) and Tianzhu (BL10). The variations between baseline and BoNTA post-injection for four months were calculated monthly as outcome measures. BoNTA injections at fixed-sites and acupoint-sites significantly reduced the migraine attack frequency, intensity, duration and associated symptoms for four months compared with placebo (p < 0.01). The efficacy of BoNTA for migraines in the acupoint-site group (93% improvement) was more significant than that in the fixed-site group (85% improvement) (p < 0.01). BoNTA administration for migraines is effective, and at acupoint-sites shows more efficacy than at fixed-sites. Further blinded studies are necessary to establish the efficacy of a low dose toxin (25 U) introduced with this methodology in chronic and episodic migraines. © 2015 by the authors; licensee MDPI, Basel, Switzerland. Source


Zhang X.,Gansu Province People Hospital | Gao J.W.,The Second Hospital of LanzhouUniversity | Jabbour P.,Thomas Jefferson University
Turkish Neurosurgery | Year: 2016

Mortality associated with the occlusion of large vessels in acute ischemic stroke is particularly high despite best available medical therapy. Early and safe revascularization of the primary occlusion is correlated with good clinical result. We report two patients with acute ischemic stroke in whom the mechanical device Penumbra System was used for thrombolysis and embolectomy. The Penumbra System provided the revascularization of the primary occlusion site in the two patients and complete revascularization was obtained. Improvement was observed in both cases on the National Institutes of Health Stroke Scale and on modified Rankin scale scores at 1 and 30 days post-procedure. Neither of the patients had intracranial hemorrhage. The Penumbra System is a valuable device as a treatment for acute ischemic stroke secondary to large vessel occlusion. Source

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