Gansu Province People Hospital

Lanzhou, China

Gansu Province People Hospital

Lanzhou, China
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Liu X.-P.,Lanzhou University | Liu H.,Gansu Province People Hospital | Wang X.-J.,Gansu Province People Hospital | Luo T.-W.,Gansu Province People Hospital | And 2 more authors.
Chinese Journal of Cancer Prevention and Treatment | Year: 2016

OBJECTIVE: This study was to evaluate the coordination of parthenolide (PTL) with cisplatin (DDP) on lung adenocarcinoma A549 cells from the level of molecular biology. METHODS: After treated by parthenolide and (or) cisplatin, the viability of cell lines was detected through microscope and MTT. The apoptosis of cell lines and cell cycle was detected by flow cytometry (FCM). The expressions of Bcl-2 and Bax mRNAs were measured by reverse transcription-PCR. RESULTS: Determined by MTT/FCM/PCR, the results showed that small doses (<10 μmol/L) of parthenolide had an effect on promoting proliferation of A549 cells, high dose (>10 μmol/L) of parthenolide could inhibit proliferation of A549 cells compared to control group or single medication group (P<0.05). The apoptotic rate (24.45%) was significantly increased in A549 cells treated with PTL in combination with cisplatin, compared with that in cisplatin-treated (3.07%) or PTL-treated (2.8%) A549 cells, and the cell cycle (81.10%) was arrested at phase G1+S vs cisplatin-treated (77.91%) or PTL-treated (70.83). The expressionlevel of Bcl-2 protein was decreased (P<0.05) and the expression level of Bax was increased (P<0.05) in a concentration-dependent manner in A549 cells treated with different drugs, and these effects (0.015±0.002, 32.175±1.651) were significantly enhanced in A549 cells treated with PTL in combination with cisplatin compared with cells treated with cisplatin (0.284±0.009, 23.873±0.76) or PTL (0.732±0.022, 2.925±0.146) alone (P<0.05). CONCLUSION: Parthenolide (10, 15, 20 μmol/L) and cisplatin (2 mg/L) have synergistic effects on inhibiting cellular proliferation and inducing cellular apoptosis of lung adenocarcinoma A549 cells, which may be associated with the increased proportion of G1+S phase cells, inducing apoptosis and decreasing the ratio of Bcl-2/Bax mRNAs. © 2016, Editorial Board of Chinese Journal of Cancer Prevention and Treatment. All right reserved.


Song X.,Lanzhou University | Song S.-K.,Lanzhou University | Zhao P.,GanSu Province People Hospital | Wei L.-M.,Lanzhou University | Jiao H.-S.,Lanzhou University
Pharmaceutical Development and Technology | Year: 2013

A sustained drug release system based on the injectable poly(lactic-co-glycolic acid) (PLGA) microspheres loaded with β-methasone was prepared for localized treatment of rheumatic arthritis. The microscopy and structure of microspheres were characterized by scanning electron microscope (SEM) and Fourier transform infrared (FTIR). The effects of various formulation parameters on the properties of microspheres and in vitro release pattern of β-methasone were also investigated. The results demonstrated that increase in drug/polymer ratio led to increased particle size as well as drug release rate. Increase in PLGA concentration led to increased particle size, but decreased burst release. The drug encapsulation efficiency increased sharply by increasing polyvinyl alcohol (PVA) concentration in the aqueous phase from 1.5 to 2.0%. β-methasone release rate decreased considerately with decreasing OP (organic phase)/AP (aqueous phase) volume ratio. Stirring rate had significantly influence on the particle size and encapsulation efficiency. Independent of formulation parameters, β-methasone was slowly released from the PLGA microspheres over 11 days. The drug release profile of high drug loaded microspheres agree with Higuchi equation with a release mechanism of diffusion and erosion, that of middle drug loaded microspheres best agreed with Hixcon-Crowell equation and controlled by diffusion and erosion as well. The low drug loaded microspheres well fitted to logarithm normal distribution equation with mechanism of purely Fickian diffusion. © 2013 Informa Healthcare USA, Inc.


Guo L.-J.,Peking University | Guo L.-J.,Gansu Province People Hospital | Tang Y.,Peking University | Guo C.-M.,Peking University | Zhang X.-H.,Peking University
Chinese Medical Journal | Year: 2010

Background Both benign prostatic hyperplasia (BPH) and primary hypertension are common in the elderly men. The purpose of this study was to investigate the possible effect of primary hypertension on the hematuria in patients with BPH. Methods All patients who underwent transurethral resection of prostate or opening operation had confirmed diagnoses of BPH histologically. comparative analysis of packet was used to analyze the incidence of hematuria in 423 BPH patients with or without hypertension. Immunostaining of CD34 and vascular endothelial growth factor (VEGF) was carried out in tissues of 50 cases of simple BPH and 50 cases of BPH accompanied with hypertension. Results The incidence of hematuria in the BPH with hypertension was significantly higher than that in the simple BPH (P <0.01). Furthermore, the incidence of hematuria in patients who had hypertension for more than 10 years was clearly higher than that in the patients who had hypertension for less than 10 years (P <0.01). Both microvessel density (MVD) based on CD34 immunostaining and VEGF expression were significantly higher in the BPH tissues of patients with hypertension than that in the simple BPH (P <0.01, P <0.05). Conclusions Long-term hypertension may significantly increase the incidence of hematuria in patients with both BPH and hypertension. Increased MVD level and VEGF expression may account for the higher incidence of hematuria in these patients.


Zhang X.,Gansu Province People Hospital | Gao J.W.,The Second Hospital of LanzhouUniversity | Jabbour P.,Thomas Jefferson University
Turkish Neurosurgery | Year: 2016

Mortality associated with the occlusion of large vessels in acute ischemic stroke is particularly high despite best available medical therapy. Early and safe revascularization of the primary occlusion is correlated with good clinical result. We report two patients with acute ischemic stroke in whom the mechanical device Penumbra System was used for thrombolysis and embolectomy. The Penumbra System provided the revascularization of the primary occlusion site in the two patients and complete revascularization was obtained. Improvement was observed in both cases on the National Institutes of Health Stroke Scale and on modified Rankin scale scores at 1 and 30 days post-procedure. Neither of the patients had intracranial hemorrhage. The Penumbra System is a valuable device as a treatment for acute ischemic stroke secondary to large vessel occlusion.


Liu Y.,CAS Lanzhou Institute of Chemical Physics | Liu Y.,University of Chinese Academy of Sciences | Sun X.,CAS Lanzhou Institute of Chemical Physics | Sun X.,University of Chinese Academy of Sciences | And 4 more authors.
Clinica Chimica Acta | Year: 2011

Background: Uric acid (UA) is the only biomedical indicator for gout in clinic that always leads to an uncertain diagnose. Due to the lack of reliable metabolites, it is now already highly desirable to diagnose gout definitely. Methods: Metabonomics was employed to screen and identify novel biomarkers of gout based on human serum and urine. High performance liquid chromatography-diode array detector (HPLC-DAD) and orthogonal signal correction partial least squares discriminate analysis (OSC-PLS-DA) were also used for metabonomics study. Results: Several potential biomarkers including uric acid, creatinine, tryptophan in serum and uric acid, creatinine, guanosine, hippuric acid in urine, were respectively screened and identified. For serum and urine, the predictive levels about the OSC-PLS-DA models of the gout and controls were 95.76% and 100%, and the correction levels about the seriousness of the disease were 90.32% and 87.5%, respectively. Conclusion: Compared with intermittent gout, the acute gout shows clearly the dysfunctions of purine, protein and glucose metabolism. The metabolizing of guanosine to UA increases the levels of UA in serum at the acute stage. Our research contributes to a better understanding of the metabolic mechanism and allowing the targeted therapy of gout at different stages. © 2011 Elsevier B.V.


Fangming G.,Tianjin Medical University | Xiaohuan W.,Gansu Province People Hospital | Guangping L.,Tianjin Medical University | Xin C.,Tianjin Medical University | Juexin F.,Yantai Hill Hospital and China France Friendship Hospital
Journal of Medical Colleges of PLA | Year: 2010

Objective: The aim of the present study was to assess the early clinical outcome and risk factors in old patients with acute ST elevation myocardial infarction (STEMI) following primary percutaneous coronary intervention (PCI). Methods: A total of 136 patients older than 60 years with STEMI who received successful PCI were included in this study. The patients were classified in 2 age groups: patients =75 years and <75 years of age. The extent of coronary artery lesions was measured by quantitative coronary artery angiography (QCA). Subjects were tracked for subsequent cardiovascular events: cardiac death, myocardial infarction, heart failure, percutaneous coronary intervention, coronary artery bypass and stroke. Results: Though the older group had a higher prevalence of adverse baseline characteristics and lower final TIMI flow than patients<75y (P<0.05), the procedural success did not make difference between the two groups. In 12 months follow-up of 136 study participants, there occurred 39 CV events: cardiac death (five patients), heart failure (nineteen patients), and stroke (six patients). Three patients received coronary bypass grafts and six patients underwent PCI. Heart failure and overall cardiovascular event rates were higher in older patients compared with those in patients<75y. The main adverse clinical events (MACE) for the old group were a little higher comparing with the younger in 12-month follow-up (P=0.029 6 and P=0.043 4). Multivariate cox analysis identified that a diagnosis of diabetes (HR 2.495, 95%CI 1.224 to 5.083, P= 0.011 8) and time from symptom(HR 1.450, 95%CI 1.143 to 1.841, P= 0.008 2) to PCI as independent predictors of CV events after adjustment of all entered baseline variables. Conclusion: Our study suggests that drug-eluting stent implantation in older patients with acute ST elevation myocardial infarction has high initial procedural success rates despite having more severe baseline risk characteristics, and to shorten the time from symptom onset to PCI may decrease cardiovascular events in old patients following PCI. © 2010 The Editorial Board of Journal of Medical Colleges of PLA.


Ling Y.,Gansu Agricultural University | Ling Y.,Lanzhou University | Xu X.,Lanzhou University | Hao J.,Gansu Province People Hospital | And 4 more authors.
Cancer Genetics and Cytogenetics | Year: 2010

The thyroid hormone receptors (TR) have three major isoforms, TRα1, TRα2, and TRβ1; these are ligand-dependent nuclear transcription factors. THRB, the gene encoding TRβ1, is considered a potential cancer suppressor. The mechanism of its inactivation is not yet clear. Aberrant silencing of THRB in breast cancer tissue and plasma by promoter hypermethylation was investigated in the present study. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to examine THRB mRNA expression in the breast cancer tissues. Methylation-specific polymerase chain reaction (MSP) combined with nested PCR was used to determine the methylation status of the THRB gene promoter region in 40 cancer tissue and 40 plasma samples from breast cancer patients. Methylation status of MSP product in plasma was also evaluated by direct sequencing. The expression of THRB mRNA in breast cancer tissues was lower than that in the normal tissues; hypermethylation was found in 32 of 40 breast cancer tissues (80%) and in 28 of 40 plasma samples (70%). Loss of THRB gene expression was associated with the CpG island hypermethylation of promoter regions. THRB gene CpG island methylation was not related to clinical pathologic parameters. Sequencing results were identical to agarose gel electrophoresis results. The present results indicate that hypermethylation of THRB as an alternative gene silencing mechanism is highly prevalent in breast cancer. Methylated tumor-specific DNA may serve as a plasma biomarker for prognosis in patients with breast cancer. © 2010 Elsevier Inc. All rights reserved.


Xia J.-F.,Lanzhou University | Xia J.-F.,CAS Lanzhou Institute of Modern Physics | Wang Z.-Z.,CAS Lanzhou Institute of Modern Physics | Liu Q.-F.,CAS Lanzhou Institute of Modern Physics | And 3 more authors.
Nuclear Science and Techniques | Year: 2014

To study cytotoxic effect of heavy ion irradiation in the plateau region, and investigate whether autophagy induced by heavy ion irradiation is cytoprotective, HeLa cells were irradiated with 350 MeV/u carbon ions beams, and the clonogenic survival was analyzed. The results showed that cell survival decreased with increasing doses. It was also found that G2/M-phase cells increased, and the autophagy-related activity was significantly higher than the control. When autophagy was blocked by 3-methyladenine in carbon-ion irradiated cells, G2/M phase arrest and the percentage of apoptosis cells were further elevated, and cell survival decreased significantly, indicating the induction of cytoprotective autophagy by carbon-ion irradiation. Our results demonstrated that autophagy induced by carbon ion irradiation provided a self-protective mechanism in HeLa cells, short-time inhibition of autophagy before carbon-ion irradiation could enhance radiation cytotoxicity in HeLa cells. © 2014, Science Press. All rights reserved.


PubMed | Lanzhou University and Gansu Province People Hospital
Type: Journal Article | Journal: Toxins | Year: 2015

Onabotulinumtoxin A (BoNTA) has been reported to be effective in the therapy for migraines. Acupuncture has been used worldwide for the treatment of migraine attacks. Injection of a small amount of drug at acupuncture points is an innovation as compared to traditional acupuncture. The purpose of this study was to evaluate and compare the effectiveness of fixed (muscle)-site and acupoint-site injections of BoNTA for migraine therapy in a randomized, double-blinded, placebo-controlled clinical trial extending over four months. Subjects with both episodic and chronic migraines respectively received a placebo (n = 19) or BoNTA (2.5 U each site, 25 U per subject) injection at fixed-sites (n = 41) including occipitofrontalis, corrugator supercilii, temporalis and trapeziue, or at acupoint-sites (n = 42) including Yintang (EX-HN3), Taiyang (EX-HN5), Baihui (GV20), Shuaigu (GB8), Fengchi (GB20) and Tianzhu (BL10). The variations between baseline and BoNTA post-injection for four months were calculated monthly as outcome measures. BoNTA injections at fixed-sites and acupoint-sites significantly reduced the migraine attack frequency, intensity, duration and associated symptoms for four months compared with placebo (p < 0.01). The efficacy of BoNTA for migraines in the acupoint-site group (93% improvement) was more significant than that in the fixed-site group (85% improvement) (p < 0.01). BoNTA administration for migraines is effective, and at acupoint-sites shows more efficacy than at fixed-sites. Further blinded studies are necessary to establish the efficacy of a low dose toxin (25 U) introduced with this methodology in chronic and episodic migraines.


PubMed | Gansu Province People Hospital
Type: Case Reports | Journal: Turkish neurosurgery | Year: 2016

Mortality associated with the occlusion of large vessels in acute ischemic stroke is particularly high despite best available medical therapy. Early and safe revascularization of the primary occlusion is correlated with good clinical result. We report two patients with acute ischemic stroke in whom the mechanical device Penumbra System was used for thrombolysis and embolectomy. The Penumbra System provided the revascularization of the primary occlusion site in the two patients and complete revascularization was obtained. Improvement was observed in both cases on the National Institutes of Health Stroke Scale and on modified Rankin scale scores at 1 and 30 days post-procedure. Neither of the patients had intracranial hemorrhage. The Penumbra System is a valuable device as a treatment for acute ischemic stroke secondary to large vessel occlusion.

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