Time filter

Source Type

Wang M.,General Hospital of Lanzhou | Wang J.,PLA Fourth Military Medical University | Kang Z.,Jilin University | Zhao Q.,General Hospital of Lanzhou | And 4 more authors.
Viral Immunology | Year: 2015

Immunodominant T cell responses are important for protection against virus challenge. However, studies screening for the immunodominant T cell responses and following their kinetics in acute Hantaan virus (HTNV) infection are very limited. Herein, the HTNV nucleocapsid protein-specific T cell responses were longitudinally screened in 15 patients with acute HTNV infection, eight of whom had a particularly severe hemorrhagic fever with renal syndrome (HFRS). An extremely impaired IFN-γ-producing T cell response was observed in patients with severe HFRS at the early stage of infection, especially to the immunodominant epitopes detected in the mild to moderate group, namely peptides N127-141, N139-153, N241-255, and N355-369. The initially insufficient T cell response to the immunodominant epitopes may play a role in influencing the severity of HTNV infection. These findings provide information that may aid the design of future vaccines against hantaviruses. © 2015 Copyright Mary Ann Liebert, Inc. Source

Wang M.,General Hospital of Lanzhou | Wang M.,PLA Fourth Military Medical University | Zhu Y.,PLA Fourth Military Medical University | Wang J.,PLA Fourth Military Medical University | And 3 more authors.
Viral Immunology | Year: 2011

Hantaan virus (HTNV) is a member of the Hantavirus genus that causes human hemorrhagic fever with renal syndrome (HFRS) in humans. The CTL response seems to play a key role in control of viral infection, but only a few HTNV epitopes recognized by the CTLs have been reported. Herein, we screened a panel of overlapping peptides covering the HTNV nucleocapsid protein by ELISPOT assays for those that can elicit IFN-γ production in vitro. Three novel CD8 + CTL epitopes, N197-205 (RYRTAVCGL), N245-253 (KLLPDTAAV), and N258-266 (GPATNRDYL), were defined on the nucleocapsid protein and were found to be restricted by various HLA alleles including A11, A24, and B7. The epitopes were highly conserved among the reported HTNV strains and other hantanviruses, including Dobrava-Belgrade virus and Seoul virus, supporting their potential use in vaccine designs. © Copyright 2011, Mary Ann Liebert, Inc. Source

Wang M.,General Hospital of Lanzhou | Wang J.,PLA Fourth Military Medical University | Wang T.,PLA Fourth Military Medical University | Li J.,PLA Fourth Military Medical University | And 4 more authors.
PLoS ONE | Year: 2013

Background: Hematological abnormalities often occur several days before kidney injury in patients with hemorrhagic fever with renal syndrome (HFRS). We aimed to investigate the prevalence and prognostic value of the early hematological markers in patients with HFRS caused by Hantaan virus (HTNV) infection. Methods: In a retrospective cohort study, we analyzed the case records of 112 patients with acute HTNV infection and evaluated the hematological markers for early prediction and risk stratification of HFRS patients with acute kidney injury (AKI). Results: Of 112 patients analyzed, 66 (59%) developed severe AKI, defined as either receipt of acute dialysis or increased serum creatinine ≥354 μmol/L. The prognostic accuracy of hematological markers, as quantified by the area under the receiver-operating-characteristic curve (AUC), was highest with the nadir platelet count (AUC, 0.89; 95% CI, 0.83-0.95), as compared with the admission platelet count (AUC, 0.84; 95% CI, 0.77-0.92), and the admission and peak leukocyte counts. The nadir platelet count correlated moderately with the levels of peak blood urea nitrogen (r = -0.616) and serum creatinine (r = -0.589), the length of hospital stay (r = -0.599), and the number of dialysis sessions that each patient received during hospital stay (r = -0.625). By multivariate analysis, decreased nadir platelet count remained independently associated with the development of severe AKI (odds ratio, 27.57; 95% CI, 6.96-109.16; P<0.0001). Conclusions: Thrombocytopenia, rather than leukocytosis, is independently associated with subsequent severe AKI among patients with acute HTNV infection. © 2013 Wang et al. Source

Discover hidden collaborations