Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement

Lanzhou, China

Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement

Lanzhou, China
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Bai J.,Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement | Bai J.,Gansu Agricultural University | Mao J.,Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement | Mao J.,Gansu Agricultural University | And 11 more authors.
BMC Genetics | Year: 2017

Background: The SnRKs (sucrose non-fermenting 1 related protein kinase) are a gene family coding for Ser/Thr protein kinases and play important roles in linking the tolerance and metabolic responses of plants to abiotic stresses. To date, no genome-wide characterization of the sucrose non-ferment 1 related protein kinase 2 (SnRK2) subfamily has been conducted in potato (Solanum tuberosum L.). Results: In this study, eight StSnRK2 genes (StSnRK2.1- StSnRK2.8) were identified in the genome of the potato (Solanum tuberosum L.) cultivar 'Longshu 3', with similar characteristics to SnRK2 from other plant species in gene structure, motif distribution and secondary structures. The C-terminal regions were highly divergent among StSnRK2s, while they all carried the similar Ser/Thr protein kinase domain. The fluorescence of GFP fused with StSnRK2.1, StSnRK2.2, StSnRK2.6, StSnRK2.7 and StSnRK2.8 was detected in the nucleus and cytoplasm of onion epidermal cells with StSnRK2.3 and StSnRK2.4 mainly associated to the nucleus while StSnRK2.5 to subcellular organelles. Expression level analysis by qRT-PCR showed that StSnRK2.1, 2.2, 2.5 and 2.6 were more than 1 fold higher in the root than in the leaf, tuber and stem tissues. The expressions of StSnRK2.3, 2.7, and 2.8 were at least 1.5 folds higher in the leaf and stem than in the root, but lower in the tuber. The expression of StSnRK2.4 was also significantly (P < 0.05) higher in leaf, stem, and tuber than in the root. From the perspective of the relative expressions of StSnRK2 genes in potato, ABA treatment had a different effect from NaCl and PEG treatments. Conclusion: In the present study, we identified and characterized eight SnRK2s in the potato genome. The eight StSnRK2s exhibit similar gene structure and secondary structures in potato to the SnRK2s found in other plant species. The relative expression of eight genes varied among various tissues (roots, leaves, tubers, and stems) and abiotic stresses (ABA, NaCl and PEG-6000) with the prolongation of treatments. This study provides valuable information for the future functional dissection of potato SnRK2 genes in stress signal transduction, plant growth and development. © 2017 The Author(s).


Qin S.,Gansu Agricultural University | Qin S.,Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement | Zhang J.,Gansu Agricultural University | Zhang J.,Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement | And 4 more authors.
Agricultural Water Management | Year: 2014

Field experiments were conducted to study the effects of different ridge-furrow plastic-mulching planting patterns (RFM) on potato ( Solanum tuberosum L.) growth, tuber yield and quality, and water use efficiency (WUE) in an arid area of Northwestern China in 2010 and 2011.Six treatments were used: (1) a flat plot without mulch (CK); (2) alternating mulched with plastic film and bare plots with no ridges (MNR); (3) completely mulched alternating wide and narrow ridges with furrow planting (CF); (4) completely mulched alternating wide and narrow ridges with ridge planting (CR); (5) alternating mulched ridges and bare plots with no ridges and with furrow planting (HF); (6) alternating mulched ridges and bare plots with no ridges and with ridge planting (HR).RFM systems greatly improved tuber yield and WUE of potato in comparsion to CK. Compared to CK, the magnitude of yield in RFM increase were 50.1-86.8% in 2010 and 36.3-60.5% in 2011, respectively. Two completely mulched treatments (CF, CR) produced the highest tuber yield. Compared to CK, the highest increase in WUE was 83.9% (CR) and 65.8% (CF) in 2010 and 2011, respectively. Evapotranspiration in RFM was significantly decreased compared to CK during the early and end growing stages. But ET in CF, CR, HF and HR became higher at vigorous growth stages (from 6 July to 27 August) due to higher transpiration, which may imply a higher ratio of transpiration/evaporation. CF and CR treatments resulted in higher dry matter and relative growth rate than other treatments, and had higher output efficiency of dry matter from aboveground to tuber. Potato in CR showed the highest tuber yield, output value, net revenue and WUE, produced tubers with good size, low percentages of green and blemished tubers, and high protein content. In conclusion, CR is the best planting pattern for rain-fed potato. © 2013 Elsevier B.V.


Mao Y.,Northwest University, China | Wang W.,Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement | Cheng N.,Northwest University, China | Li Q.,Northwest University, China | Tao S.,Northwest University, China
Gene | Year: 2013

Local secondary structures in coding sequences have important functions across various translational processes. To date, however, the local structures and their functions in the early stage of translation elongation remain poorly understood. Here, we surveyed the structural stability in the first 180 nucleotides of the coding sequence of 27 species using computational method. We found that the structural stability in the 30-80 nucleotide interval was significantly higher than that in other regions in eukaryotes and most prokaryotes. No significant correlation between local translation efficiency and structural stability was observed, suggesting that this structural region has undergone selection pressure directly to maintain high stability. Furthermore, ribosome was blocked by this region, providing an opportunity for co-translational regulation. Remarkably, in eukaryotes, we found that mRNAs with higher structural stability in the 30-80 nucleotide interval tended to encode the secreted proteins. Overall, our results revealed a previously unappreciated correlation between structural stability and protein localization. © 2012 .


Yin Y.,Gansu Agricultural University | Bi Y.,Gansu Agricultural University | Li Y.,Gansu Agricultural University | Li Y.,Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement | And 2 more authors.
International Journal of Food Science and Technology | Year: 2012

The effects of thiamine (vitamin B 1) against Alternaria rot caused by Alternaria alternata and its possible mechanism in harvested Asian pear fruit were investigated. Thiamine strongly inhibited spore germination and mycelial growth of A. alternata in vitro. Thiamine at a concentration of 100mm effectively inhibited the development of Alternaria rot, enhanced the activities of defence-related enzymes, such as phenylalanine ammonia-lyase (PAL), polyphenoloxidase (PPO) and peroxidase (POD), and increased the content of flavonoids, phenolics and lignin. Thiamine also affected reactive oxygen metabolism of the pear fruit by increasing its production and H 2O 2 content, and enhancing the activities of major detoxifying enzymes, such as superoxide dismutase (SOD). These findings suggest that the effects of thiamine on Alternaria rot in pear fruit may be associated with its direct fungitoxic property against the pathogens, and the elicitation of biochemical defence responses in the fruit. © 2012 The Authors. International Journal of Food Science and Technology © 2012 Institute of Food Science and Technology.


Ru Z.,Vegetable Institute of Gansu Academy of Agricultural science | Ru Z.,Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement | Di W.,Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement | Di W.,Gansu Agricultural University
African Journal of Biotechnology | Year: 2012

One hundred and forty six (146) isolates of Trichoderma spp. were obtained from rhizosphere soils of potato plants in the middle areas of Gansu Province, China. By means of dual culture method, they were examined for antagonism against Fusarium sambucinum, which causes potato dry rot. Ten of the isolates were found to be evidently antagonistic to the pathogen. Based on morphological characteristics and molecular analyses, the antagonistic isolates were identified as Trichoderma harzianum Rifai, Trichoderma longibrachiatum Rifai, Trichoderma atroviride Karsten and Trichoderma virens. Among these isolates, D-3-1 (T. longibrachiatum) showed the strongest inhibition of the growth of Fusarium sambucinum. © 2012 Academic Journals.


Wang C.,Gansu Agricultural University | Wang C.,Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement | Wu R.,Gansu Agricultural University | Li F.-D.,Gansu Agricultural University | And 5 more authors.
Virus Genes | Year: 2011

Determination of the transcription level of cellular prion protein (PrPC) is essential for understanding its role in organisms and revealing mechanism of susceptibility and resistance to scrapie. However, the expression of prion protein (PrP) mRNA in sheep has not been quantified in great detail in digestive tract which is important during scrapie spread through oral route. Herein, we report on measurement of sheep PrP mRNA using absolute quantitative real-time RT-PCR. Total RNA was isolated from five different regions of the central nervous system (CNS), four regions of lymphoid system, eleven regions of digestive tract, and two reproductive organ tissues of eight sheep of two different genotypes (ARR/ARQ and ARH/ARQ) and PrP mRNA was quantified by real-time RT-PCR using molecular beacon. The results showed that highest levels of PrP mRNA were expressed in thalamus and cerebrum (P < 0.01) of CNS examined, followed by cerebellum, spinal cord, and brain stem. In peripheral organs examined, lymph tissue showed moderate level of PrP expression similar to that in digestive tract and reproduction organs. PrP expression levels in the same tissue of different genotype sheep had significant variation. Our study provided the first detail, tissue-specific and genotype-specific data of PrP mRNA expression in sheep for further studies of pathogenesis of prion diseases. © 2011 Springer Science+Business Media, LLC.


Cui T.,Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement | Cui T.,Gansu Provincial Key Laboratory of Aridland Crop Science | Cui T.,Gansu Agricultural University | Bai J.,Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement | And 8 more authors.
New Zealand Journal of Crop and Horticultural Science | Year: 2014

Red light (10-12 lx) has been shown to induce the accumulation of steroidal glycoalkaloids (SGA) in potato (Solanum tuberosum) tubers. This study was to analyse the impact of genotypes, red light illumination and duration of incubation on SGA biosynthesis at the transcriptional level. The microtubers of wild species S. chacoense and cultivated potato varieties Shepody, Favorita, Longshu-3 and Zhuangshu-3 were tested. After 24 h incubation in the dark, the genes coding for hydroxymethylglutaryl coenzyme A reductase (hmg1, hmg2) and squalene synthase (pss1) remained relatively stable in S. chacoense and Longshu-3. The gene coding for vetispiradiene synthase (pvs1) was significantly induced in Favorita and Longshu-3. The expressions of sgt1 (solanidine galactosyl transferase) and sgt3 (rhamnosyltransferase) were induced in all tested varieties except S. chacoense. The transcript abundance of hmg1, hmg2, sgt1 and sgt3 was increased with red light illumination and incubation duration, but pvs1 was decreased. The results imply a feedback regulation system at the transcriptional level in SGA biosynthesis during red light illumination. © 2014 The Royal Society of New Zealand.


Meng Y.,Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement | Meng Y.,Lanzhou University | Ren P.,Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement | Ren P.,Lanzhou University | And 12 more authors.
Journal of Agricultural Science and Technology | Year: 2016

The yield performance of 23 barley (Hordeum vulgare L.) genotypes in sixteen test environments across a barley growing region of China was evaluated. The experiment was conducted using a randomized complete block design with three replicates, in two cropping seasons (2010-2011, in the South; 2012-2013, in the North). The GGE biplot was applied to analyze the data obtained in the multi-environment trials. The results indicated that either the North or South test sites could be grouped into three possible mega-environments, the best- performing and candidate genotypes for the North and South were G7 (Zhongsimai1), G5 (08B26), G17 (G231M004M), and G13 (Zhe3521), respectively. Among the sixteen test environments, E6 (Shihezi) and E12 (Yancheng) had the greatest discriminating ability, while E1 (Haerbing), E4 (Shang kuli), E8 (Wuhan), and E16 (Chengdu) could be dismissed from the future trials due to the similarity of their ability of discrimination and representation. © 2016, Tarbiat Modares University. All Rights reserved.


Yin Y.,Gansu Agricultural University | Yin Y.,Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement | Li Y.-C.,Gansu Agricultural University | Li Y.-C.,Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement | And 5 more authors.
Agricultural Sciences in China | Year: 2010

The effectiveness of postharvest β-aminobutyric acid (BABA) treatment was studied for inducing resistance against dry rot caused by Fusarium sulphureum in tubers and slices of two potato cultivars (resistant cultivar Shepody and susceptible cultivar Xindaping). The results showed that BABA at 100 mmol L-1 significantly reduced lesion diameter in inoculated both tubers and slices. The chemical at 100 mmol L-1 showed an effective reduction in infection ability of F. sulphureum inoculated 48 and 72 h after treatment in slices of resistant cultivar, and 72 and 96 h in susceptible ones. BABA increased the activitives of peroxidase (POD), polyphenoloxidase (PPO) and phenylalanine ammonialyase (PAL), and accumulated the contents of lignin, flavonoids and phenolics in slices. The resistant cultivar had a stronger resistant response than the susceptible one. These findings suggest that the BABA treatment can induce the resistance in potato tubers, however, the inducing degree depends on the original level of resistance present in each cultivar. © 2010 Chinese Academy of Agricultural Sciences.


Mao Y.,Northwest University, China | Li Q.,Northwest University, China | Wang W.,Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement | Liang P.,Northwest University, China | Tao S.,Northwest University, China
Genome Biology and Evolution | Year: 2013

Various regulatory elements in messenger RNAs (mRNAs) carrying the secondary structure play important roles in a wide range of expression processes. Numerous recent works have focused on the discovery of these functional elements that contain the conserved mRNA structures. However, to date, regions with high structural stability have been largely overlooked. In this study, we defined high stability regions (HSRs) in the coding sequences (CDSs) in bacteria based on the normalized folding free energy. We found that CDSs had high number of HSRs, and these HSRs showed high structural context robustness compared with random sequences, indicating a direct selective constraint imposed on HSRs. A reduced ribosome speed was detected near the start position of HSR, implying a possibility that HSR acted as obstacle to drive translational pausing that coordinated protein synthesis. Interestingly, we found that genes with high HSR density were enriched in the processes of translation, protein folding, and cell division. In addition, essential genes exhibited higher HSR density than nonessential genes. Overall, our study presented the previously unappreciated correlation between the number variation of HSRs and cellular processes. © The Author(s) 2013.

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