Wang Q.,Gansu College of Traditional Chinese Medicine |
Xue Y.,San Ai Tang Hospital
Oncology Letters | Year: 2015
Renal-type clear cell carcinoma of the prostate is a rare and novel tumor that has only been identified in recent years. The present study describes a lesion in the prostate of a 64-year-old male with a two-year history of urinary frequency, urgency and difficulty, who was admitted to the San Ai Tang Hospital for benign prostatic hyperplasia, and subsequently underwent transurethral resection of the prostate. In total, 12 g of tissue was resected, which demonstrated morphological and immunohistochemical similarities to clear cell carcinoma of the kidney. Ultrasound inspection and computed tomography revealed prostate enlargement. Although no renal-enclosed mass was identified, metastatic lesions were revealed in the lungs, sternum and clavicles. In addition, right pleural thickening and a small amount of effusion in the pleural cavity were detected. Clear cell carcinoma was identified throughout the prostate, with surrounding regions of ordinary-type prostatic adenocarcinoma (Gleason score, 4+4). The urinary bladder exhibited no dysplasia or neoplasia. It was therefore concluded that the tumor represented a primary renal-type clear cell carcinoma that had arisen in the prostate. To the best of our knowledge, this type of extra-renal tumor has only been reported in three other previous studies. © 2015, Spandidos Publications. All rights reserved.
Zhang S.,Lanzhou Industry Research Institute |
Gao S.,Gansu College of Traditional Chinese Medicine |
Gao G.,Lanzhou Industry Research Institute
Applied Biochemistry and Biotechnology | Year: 2010
A study of the cross-linking of β-galactosidase on magnetic beads is reported here. The magnetic beads were prepared from artemisia seed gum, chitosan, and magnetic fluid in the presence of a cross-linking regent (i.e., glutaraldehyde). The reactive aldehyde groups of the magnetic beads allowed the reaction of the amino groups of the enzymes. The animated magnetic beads were used for the covalent immobilization of β-galactosidase. The effect of various preparation conditions on the activity of the immobilized β-galactosidase, such as immobilizing time, amount of enzyme, and the concentration of glutaraldehyde, were investigated. The influence of pH and temperature on the activity and the stability of the enzyme, both free and immobilized, have been studied. And o-nitrophenyl-β-d-galactopyranoside (ONPG) was chosen as a substrate. The β-galactosidase immobilized on the magnetic beads resulted in an increase in enzyme stability. Optimum operational temperature for immobilized enzyme was 10 °C higher than that of free enzyme and was significantly broader. © 2009 Humana Press.
Yao N.,Gansu College of Traditional Chinese Medicine |
Wang X.,Xi'an Jiaotong University
American Journal of Chinese Medicine | Year: 2014
Oxymatrine, extracted from the herb Sophora alopecuraides L., was investigated to determine its anti-HBV immunomodulatory mechanism in vitro. Human peripheral lymphocytes were isolated from heparinized whole blood from 48 chronic hepatitis B (CHB) patients. The lymphocytes from each patient were divided into two groups according to pretreatment or no pretreatment with Oxymatrine in vitro. We examined the changes of expression and function of the toll-like receptor 9 (TLR9) signal transduction pathway in the peripheral lymphocytes with different treatment methods and investigated the synergism of Oxymatrine and the TLR9 ligand on antiviral cytokine secretions in vitro. The data showed Oxymatrine could induce antiviral cytokine secretions directly from the peripheral lymphocytes. For the TLR9 signal pathway, Oxymatrine not only augmented the expressions of TLR9 signal transduction molecules, but also activated the TLR9 signal function. This study has clearly demonstrated that TLR9 ligand could stimulate peripheral lymphocytes that have been pretreated with Oxymatrine. Furthermore, the quantity of antiviral cytokines secreted by the pretreated lymphocytes was greater than that of those without pretreatment. The interaction between the Oxymatrine and the TLR9 ligand appears to be synergistic. This study suggests Oxymatrine could be a strong immunomodulator, influence TLR9 signaling transduction, and synergistically improve the immune efficacy of the TLR9 ligand against CHB. © 2014 World Scientific Publishing Company & Institute for Advanced Research in Asian Science and Medicine.
Guo X.,Northwest Normal University |
Shang D.,Gansu College of Traditional Chinese Medicine
Mathematical Problems in Engineering | Year: 2013
The approximate solution of n th-order fuzzy linear differential equations in which coefficient functions maintain the sign is investigated by the undetermined fuzzy coefficients method. The differential equations is converted to a crisp function system of linear equations according to the operations of fuzzy numbers. The fuzzy approximate solution of the fuzzy linear differential equation is obtained by solving the crisp linear equations. Some numerical examples are given to illustrate the proposed method. It is an extension of Allahviranloo's results. © 2013 Xiaobin Guo and Dequan Shang.
Guo X.,Northwest Normal University |
Shang D.,Gansu College of Traditional Chinese Medicine
Advances in Fuzzy Systems | Year: 2012
The fuzzy symmetric solution of fuzzy matrix equation A X = B , in which A is a crisp m m nonsingular matrix and B is an m n fuzzy numbers matrix with nonzero spreads, is investigated. The fuzzy matrix equation is converted to a fuzzy system of linear equations according to the Kronecker product of matrices. From solving the fuzzy linear system, three types of fuzzy symmetric solutions of the fuzzy matrix equation are derived. Finally, two examples are given to illustrate the proposed method. Copyright © 2012 Xiaobin Guo and Dequan Shang.
Li Y.,Gansu College of Traditional Chinese Medicine |
Zhuo D.,Gansu College of Traditional Chinese Medicine
Cancer Research and Clinic | Year: 2014
Objective: To compare the efficacy and toxicity of SOX and FOLFOX4 regimen in the treatment of advanced gastric cancer. Methods: Total of 32 patients with advanced gastric cancer proved pathologically were randomly divided into 2 groups: 16 patients received SOX regimen [oxaliplatin 1.30 mg/m2 as a 2-hour infusion on day 1, S-1 capsules 80 mg/m2·d) twice a day per oral from day 1 to day 14 every 3 weeks], the other 16 patients received FOLFOX4 regimen [oxaliplatin 85 mg/m2 as a 2 hour infusion on day 1 and a 2 hour infusion of LV 200/(m2·d) followed by a 5-Fu bolus 400/(m2·d) and 22 hour infusion 600/(m 2·d) for 2 consecutive days every 2 weeks]. Efficacy was evaluated at least 2 cycles. Results: The response rates of SOX group and FOLFOX4 group were 56.25% (9/16) and 43.57% (7/16), the disease control rates were 81.25% (13/16) and 75.00% (12/16). The median progress free survival times were 7.2 months and 5.6 months, the median overall survival times were 9.4 months and 7.9 months. The main toxicity in the two groups was hematological toxicity, nausea/vomiting, and peripheral neuropathy. The main grade 3 hematologic toxicity was neutropenia, with no significant difference between them [12.50% (2/16) vs 31.25% (5/16), P = 0.394]. Conclusions: Both SOX and FOLFOX4 regimens are effective for patients with advanced gastric cancer. Compared with FOLFOX4 regimen, SOX regimen is better tolerated for advanced gastric cancer with longer progress free survival and overall survival.
Cheng F.,Gansu College of Traditional Chinese Medicine
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2011
To study the active ingredients of analgesic and anti-inflammatory from Meconopsis quintuplinervia, Tracking the active ingredients of such medicines. The compounds Meconopsis quintuplinervia,were separated with chromatography and its chemical structure was elucidated by means of MS, NMR spectroscopy methods respectively. Five compounds were obtaine as O-methylflavinantine (I), flavinantin (II), tricin (III), quercitrin (IV) and methyl linoleate (V). Compounds I is obtained from the plant for the first time.
Chen L.,South-Central University for Nationalities |
Zhao L.,Gansu College of Traditional Chinese Medicine |
Zhang C.,China Pharmaceutical University |
Lan Z.,Hubei University of Chinese Medicine
Inflammation | Year: 2014
In the previous study, the anti-inflammatory effect of p-cymene had been found. In this study, we investigated anti-inflammatory effects of p-cymene on acute lung injury using lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. The cell counting in the bronchoalveolar lavage fluid (BALF) was measured. The animal lung edema degree was evaluated by wet/dry weight (W/D) ratio. The superoxidase dismutase (SOD) activity and myeloperoxidase (MPO) activity was assayed by SOD and MPO kits, respectively. The levels of inflammatory mediators including tumor necrosis factor alpha (TNF-α), IL-1β, and IL-6 were assayed by enzyme-linked immunosorbent assay method. The pathological changes of the lung tissues were observed by hematoxylin and eosin staining. The inflammatory signal pathway-related protein levels of NF-κB were measured using Western blotting. The data showed that treatment with the p-cymene markedly attenuated inflammatory cell numbers in the BALF, decreased NF-κB protein level in the lungs, improved SOD activity, and inhibited MPO activity. Histological studies demonstrated that p-cymene substantially inhibited LPS-induced neutrophils in the lung tissue compared with the model group. The results indicated that p-cymene had a protective effect on LPS-induced ALI in mice. © 2013 Springer Science+Business Media New York.
Sa R.N.,Gansu College of Traditional Chinese Medicine
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2012
To study the variation of the biomass of the root and active components of Angelica sinensis during different growth periods. 27 batches of Angelica sinensis were harvested from different growth periods, and the biomass of underground parts were determined; The Gas Chromatography-Mass Spectrometry (GC-MS) was used for determining the contents of Z-ligustilide and n-Butylidenephthalide in essential oil of Radix Angelicae Sinensis. The average contents of n-Butylidenephthalide and Z-ligustilide were more than 1% and 40% in the total essential oil of Radix Angelicae sinensis respectively. Their contents showed larger difference during different growth period. The contents of Z-ligustilide and n-Butylidenephthalide of Radix Angelicae Sinensis is closely related to their growth period.
Liu A.G.,Gansu College of Traditional Chinese Medicine
Zhen ci yan jiu = Acupuncture research / [Zhongguo yi xue ke xue yuan Yi xue qing bao yan jiu suo bian ji] | Year: 2013
To observe the effect of electroacupuncture (EA) at "Sanyin" acupoints (bilateral "Jiayin" and "Zhongyin") on the cellular immune function of rats with chronic abacterial prostatitis (CAP), so as to explore its mechanism underlying relieving CAP. Forty male Wistar rats were randomly divided into four groups: control, model, medication (Cernilton) and EA (n = 10 rats/group). The CAP model was made by injection of allogeneic prostatein purification liquid + Freund's complete adjuvant (1:1) and diphtheria, pertussis, tetanus (DPT) vaccine (0.5 mL). EA was applied to bilateral "Jiayin" acupoints (located at the bilateral groins at the horizontal level of the upper border of the pubic symphysis) and "Zhongyin" [the midpoint between the "Huiyin" (CV 1) and the scrotum root, punctured with filiform needle] for 20 min, once daily for two therapeutic courses (15 days/course, one day break between the two courses). Rats of the medication group were given with oral administration of Cernilton (13 mg/kg) once daily for 15 days. Following killing the rats under deep anesthesia, the prostate wet weight, and prostate index (prostate weight/body weight) were determined. Pathological changes of the prostate tissue were examined by light microscope after sectioning and haematoxylin (HE) staining. Plasma levels of CD4+ Th- and CD8+ T-lymphocytes were assayed with flow cytometry. Compared with the control group, the morphological structure of prostate tissues of rats in the model group was severely damaged (proliferation of the prostate epithelium and inflammatory cell infilitration), which was relatively milder in the EA group. The plasma CD4+ level and CD4+/CD8+ ratio were significantly lower in the model group than in the control group (P < 0.01, P < 0.05). Compared with the model group, plasma CD4+ levels were significantly increased in both medication and EA groups (P < 0.05, P < 0.01), and the CD4+ level of the EA group was considerably higher than that of the medication group (P < 0.05). The ratio of CD4+/CD8+ in the EA group was remarkably higher than that in the model group (P < 0.05). No significant differences were found among the four groups in the prostate weight, prostate index and plasma CD8+ levels (P > 0.05). EA stimulation of "Sanyin" points is beneficial to relieve chronic abacterial prostatitis, which may contribute to its effect in strengthening the cellular immune function via upregulation of plasma CD4+ and CD4+/ CD8+ levels.