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Yu F.-R.,Gansu Institute of Political Science and Law | Liu Y.,Gansu Academy of Medical science | Cui Y.-Z.,Northwest Normal University | Chan E.-Q.,Gansu Provincial Peoples Hospital | And 3 more authors.
American Journal of Chinese Medicine | Year: 2010

The present study investigated the effects of a flavonoid extract from Cynomorium songaricum on the swimming endurance of rats by measuring changes of free radical scavenging enzymes, such as CuZn-SOD (copper, zinc-superoxide dismutase) and GSH-px (glutathione peroxidase), and body weights. Significant and dose-dependent antioxidant and anti-fatigue effects of flavonoids (rutin, catechin and isoquercitrin) on swimming rats were observed during 10 days of swimming exercise. After treatment with the flavonoid extract at doses of 0.5, 1.0, and 2.0 g/kg body weight, the CuZn-SOD and GSH-px activities in swimming rats were increased by 1.4%, 3.3%, 4.1% and 112.2%, 208.7%, 261.7%, respectively, while the levels of MDA (malondialdehyde) were decreased by 64.7%, 79.4%, and 86.4% respectively. Furthermore, the average body weight and the total swimming time were increased by 3.1%, 8.8%, 10.6%, and 7.7%, 34.5%, 61.5%, respectively. Our experimental results suggest that flavonoid supplementation could not only reduce free radical formation and scavenge free radicals, but also enhance endurance exercise performance by reducing muscle fatigue. © 2010 World Scientific Publishing Company & Institute for Advanced Research in Asian Science and Medicine.

Zheng X.,Lanzhou University | Dong F.,Gansu Academy of Medical science | Yang J.,Lanzhou University | Duan X.,Lanzhou University | And 3 more authors.
Journal of Radioanalytical and Nuclear Chemistry | Year: 2011

This work reports the synthesis and preliminary biodistribution results of [131I]SIB-PEG4-CHC in tumor-bearing mice. The tributylstannyl precursor ATE-PEG4-CHC was synthesized by conjugation of ATE to amino pegylated colchicine NH2-PEG4-CHC. [ 131I]SIB-PEG4-CHC was radiosynthesized by electrophilic destannylation of the precursor with a yield of ~44%. The radiochemical purity (RCP) appeared to be >95% by a Sep-Pak cartridge purification. [ 131I]SIB-PEG4-CHC was lipophilic and was stable at room temperature. Biodistribution studies in tumor-bearing mice showed that [ 131I]SIB-PEG4-CHC cleared from background rapidly, and didn't deiodinate in vivo. However, the poor tumor localization excluded it from further investigations as a tumor-targeted radiopharmaceuticals. © 2010 Akadémiai Kiadó, Budapest, Hungary.

Wang J.,Bioactive Products Engineering Research Center For Gansu Distinctive Plants | Wang J.,Northwest Normal University | Guo H.,Gansu Academy of Medical science | Zhang J.,Bioactive Products Engineering Research Center For Gansu Distinctive Plants | And 8 more authors.
Carbohydrate Polymers | Year: 2010

Sulfated polysaccharides exerted potent biological property which was relative to degree of sulfation (DS), Mw, substitution position and chain conformation. In the present study, sulfated derivatives of Artemisia sphaerocephala polysaccharide (ASP) with different DS were synthesized by chlorosulfuric acid/pyridine method. FT-IR and 13C NMR analysis indicated that C-6 substitution was predominant in sulfated ASP (SASP) compared with other positions at C-2. In the sulfation reaction, a sharp decrease in Mw was observed in SASP. The df values from 1.96 to 2.77 indicated that the -SO3H groups leading to the relatively expanded conformation of SASP. Antioxidant assays showed that SASP had better antioxidant activities. The data obtained in vitro models indicated that high DS and moderate Mw showed the best antioxidant capacities. Crown Copyright © 2010.

Yuan J.C.,Northwest Normal University | Xie X.L.,Northwest Normal University | Zeng X.W.,Gansu Academy of Medical science | Guo H.Y.,Gansu Academy of Medical science | Miao C.P.,Northwest Normal University
Chinese Chemical Letters | Year: 2012

To develop new tumor targeting macromolecular conjugates, poly(HPMA)-SD-APMA-DTPA (HPMA: N-(2-hydroxypropyl)-methacrylamide; APMA: N-(3-aminopropyl)methacrylamide; DTPA: diethylenetriaminepentaacetic acid; SD: sulfadiazine) was synthesized and characterized. The poly(HPMA)-SD-DTPA conjugates were radiolabeled with the radionuclide 99mTc and tested for uptake by cultured H22 cells in vitro. DTPA- 99mTc (radiotracer 1) and poly(HPMA)-DTPA- 99mTc (radiotracer 2) were also synthesized and characterized for comparison. The uptake of poly(HPMA)-SD-DTPA- 99mTc (radiotracer 3, 34.76%) was significantly higher than that of poly(HPMA)-DTPA- 99mTc (16.40%), indicating that uptake of the poly(HPMA)-SD-DTPA- 99mT was active binding. The uptake of poly(HPMA)-DTPA- 99mTc was significantly higher than that of DTPA- 99mTc (2.98%), suggesting that uptake of the poly(HPMA)-DTPA- 99mT was passive binding. The data suggest that the poly(HPMA)-SD-APMA-DTPA conjugates might be useful as tumor targeting macromolecular conjugates. © 2012 Jian Chao Yuan.

Yuan J.,Northwest Normal University | Li J.,Northwest Normal University | Jia Z.,Northwest Normal University | Song F.,Northwest Normal University | And 2 more authors.
Journal of Bioactive and Compatible Polymers | Year: 2012

Recent studies have identified that sulfadiazine derivatives can be concentrated in the hepatocellular carcinoma tissue. Herein, we report the synthesis, characterization, and evaluation of a novel sulfamethazine N-(2-hydroxypropyl)methacrylamide copolymer conjugates for tumor targeting. N-(3-Aminopropyl)methacrylamide-diethylenetriaminepentaacetic acid monomer 1, methacryloyl-sulfamethazine monomer 2, poly(N-(2-hydroxypropyl)methacrylamide)- sulfamethazine-diethylenetriaminepentaacetic acid conjugate 4, and poly(N-(2-hydroxypropyl)methacrylamide)-sulfamethazine- diethylenetriaminepentaacetic acid-99mTc were successfully synthesized and characterized. Poly(N-(2-hydroxypropyl)methacrylamide)- diethylenetriaminepentaacetic acid conjugate 3, diethylenetriaminepentaacetic acid-99mTc, and poly(N-(2-hydroxypropyl)methacrylamide)- diethylenetriaminepentaacetic acid-99mTc were also synthesized and characterized for comparison (99mTc: metastable technetium-99). A 24-h necropsy data in the hepatocellular carcinoma tumor model showed significantly higher (p < 0.001) tumor localization for poly(N-(2-hydroxypropyl)methacrylamide)-sulfamethazine- diethylenetriaminepentaacetic acid-99mTc (4.82%ID/g ± 0.46%ID/g (percentage injected dose per gram tissue)) compared with poly(N-(2- hydroxypropyl)methacrylamide)-diethylenetriaminepentaacetic acid-99mTc (2.69%ID/g ± 0.15%ID/g) and diethylenetriaminepentaacetic acid-99mTc (0.83%ID/g ± 0.03%ID/g). Moreover, higher tumor/organ ratios for poly(N-(2-hydroxypropyl)methacrylamide)-sulfamethazine- diethylenetriaminepentaacetic acid-99mTc indicated reduced extravasation of the targeted polymeric conjugates in normal tissues. Thus, the poly(N-(2- hydroxypropyl)methacrylamide)-sulfamethazine-diethylenetriaminepentaacetic acid can potentially be used as a macromolecular targeting carrier for hepatocellular carcinoma in mice. © The Author(s) 2012.

Zhang J.M.,Gansu Academy of Medical Science
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2010

To study the chemical constituents from pine needles of Cedrus deodara. Chemical constituents were isolated from 95% ethanol extract by silica gel and Sephadex LH-20 column chromatography. The structures were elucidated through spectroscopic analysis. The compounds were identified as 10-nonacosanol (1), dibutyl phthalate (2), protocatechuic acid (3), phthalic acid bis-(2-ethylhexyl) ester (4), (E)-1-O-p-coumaroyl-beta-D-glucopyranoside (5), 5-p-trans-coumaroylguinic acid (6). Compounds 1- 6 are obtained from this genus for the first time.

Liu D.-Y.,Gansu Academy of Medical Science | Shi X.-F.,Gansu Academy of Medical Science | Wang D.-D.,Gansu Academy of Medical Science | He F.-J.,Gansu Academy of Medical Science | And 2 more authors.
Chemistry of Natural Compounds | Year: 2011

Two new myricetin glycosides, myricetin-3-O-(6″-O-E-p-coumaroyl)- α-D-glucocopyranoside (1) and 3′,5′-di-O-methylmyricetin-3-O- (6″-O-acetyl)-α-D-glucopyranoside (2), and three known flavonoids, myricetin (3), cedrin (4), and 2R,3R-dihydromyricetin (5), were isolated from the pine needles of Cedrus deodara. Their structures were elucidated on the basis of extensive spectroscopic analysis and chemical evidence. © 2011 Springer Science+Business Media, Inc.

Ma Q.H.,Gansu Academy of Medical Science
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2012

To study the chemical constituents from Patrinia scabra Bunge. Chemical constituents were isolated and purified by silica gel and Sephadex LH-20 column chromatography. Their structures were elucidated on the basis of spectroscopic analysis and chemical evidence. Seven compounds were isolated and purified. Their structures were identified as villosol (I), patriscabrol (II), protocatechuic acid (III), beta-daucosterol (IV), oleic acid (V), beta-sitosterol (VI), erucic acid (VII). Compounds I, III, V, VII are isolated from this plant for the first time.

Shi X.F.,Gansu Academy of Medical Science
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2012

To study the chemical constituents of the dichloromethane extracted from pine needles of Cedrus deodara. Compounds were isolated and purified from the dichloromethane extract of pine needles by chromatography on silica gel and Sephadex LH-20. Their structures were identified on the basis of spectroscopic analysis and physicochemical property. Nine compounds were isolated and purified. Their structures were identified as stigmasterol (1), oleanolic acid (2), parahydroxybenzaldehyde (3), beta-sitosterol (4), syringaresinol (5), daucosterol (6), p-hydroxybenzoic acid (7), gallicin (8) and gallic acid (9). Compounds 1-3, 5 -9 are isolated from pine needles of this genus for the first time.

Chen X.-Z.,Gansu Academy of Medical science
Chinese Journal of Oncology | Year: 2012

Objective: To explore the pathogenesis of tumors by blocking the normal differentiation process of stem cells. Methods: Bone marrow mesenchymal stem cells (BMSCs) from rats were isolated, cultured and purified by whole bone marrow adherence method. The rat BMSCs were induced to differentiate into adipocytes with dexamethasone, insulin and indomethacin. Blockage of the differentiation process was induced by 3-methylcholanthrene (3-MC). Results: The differentiation experiment showed that at 30 days after the induction, oil red 0 staining-positive cells occurred with increased intracytolasmic lipid droplets, characteristic for adipocytes. The differentiation blockage experiment showed that at 30 days after induction, the deposits of oil red 0 staining-cytoplasmic lipid droplets was significantly reduced, indicating that the blocked cells were adipocytes, but not fully differentiated. Morphological identification showed that cell contact inhibition disappeared, abnormal cell nuclei, increased number of micronucleus aberration and karyotype abnormalities, indicating that malignant transformation of the stem cells occurred after the differentiation blockage. Conclusions: The results of this study show a blockage of the differentiation of that stem cells at the intermediate phase, and a tendency of malignant transformation of the stem cells. The results of our study provide new evidence that cancer stem cells may be originated by suppression of stem cell differentiation.

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