Patel A.,Ganpat University
2016 International Conference on Signal and Information Processing, IConSIP 2016 | Year: 2016
Edges are instant change in intensity value of pixels in the digital image. Image edge detection converts original image in to binary image. This binary image contains information about edges of digital image. Recently Ant Colony Optimization Meta-heuristic algorithm is used for the image edge detection. Ant colony optimization algorithm is inspired from the behaviour of real ants. From this ACO based algorithm we get pheromone matrix at end, which contains the information about edge pixels of original image. Discrete wavelet transform is used to decompose original image in for sub-bands. ACO algorithm is separately applied on that four sub-bands and reconstructed image of these four components give better result than single ACO edge detected image. Dual tree complex wavelet transform is advance than discrete wavelet transform. Problems in DWT like overemphasized, distortion and less visible image are removed using DT-CWT with ACO algorithm. It has higher orientation property. K-means clustering algorithm and Fuzzy C-means algorithms are used to decide whether pixels in image are edge or not. So that proposed algorithms which are ACO + DT-CWT with K-means clustering algorithm and Fuzzy C-means algorithm, they are able to find all possible edges from image and give better result compared to other edge detection algorithms. © 2016 IEEE.
Patel S.A.,Ganpat University
International Journal of Pharmaceutical Research | Year: 2012
A simple, sensitive, accurate, precise, economical and rapid visible spectrophotometric method has been developed for the estimation of linezolid in tablets. The method is based on the reaction of linezolid with Folin-Ciocalteu reagent in presence of 20% sodium carbonate solution, giving blue colour chromogen, which shows maximum absorbance at 765 nm against reagent blank. The chromogen obeyed Beer's law in the concentration range of 10-100 μg/ml for linezolid. The results of the analysis have been validated statistically and by recovery studies.
Sharma R.N.,Ganpat University |
Pancholi S.S.,Babria Institute of Pharmacy
Scientia Pharmaceutica | Year: 2014
Carotid intima-media thickness is used as a surrogate marker for cardiovascular complications in diabetes mellitus. The combination of atorvastatin and pioglitazone was found to be effective in reducing the thickness of the carotid intima-media layer. The method of RP-HPLC coupled with a diode array detector (DAD) was developed for the pharmacokinetic interaction study of atorvastatin with pioglitazone and cholestyramine, respectively, in Wistar rats. Atorvastatin (ATR) and pioglitazone (PIO) were resolved on a C18 column with a mobile phase composed of 48% methanol, 19% acetonitrile, and 33% 10 mM ammonium formate (v/v/v; pH 3.5±0.3, by formic acid) and a 260 nm detection wavelength on the diode array detector. The method was validated according to international standards with good reproducibility and linear response; mean (r) 0.9987 and 0.9972 to ATR and PIO, respectively. The coefficients of variation of intra- and interassay precision ranged between 4.95-8.12 and 7.29- 9.67, respectively. Pharmacokinetic parameters were determined in rats following an oral administration of atorvastatin in the presence and absence of pioglitazone and also with cholestyramine. Compared with the control given atorvastatin alone, the Cmax and AUC of atorvastatin were merely unchanged in rats with the co-administration of pioglitazone, while they decreased by nearly 21 and 15%, respectively, with the concurrent use of cholestyramine. There were no significant changes in Tmax and the plasma half-life (T1/2) of atorvastatin in both cases. The performed experiment demonstrated that the presented method was suitable for the estimation and pharmacokinetic interaction study of atorvastatin with pioglitazone and cholestyramine in Wistar rat plasma. ©Sharma and Pancholi.
Sharma R.N.,Ganpat University |
Pancholi S.S.,Babria Institute of Pharmacy
Acta Pharmaceutica | Year: 2012
A simple RP-HPLC method for the quantification of valsartan (VAL), amlodipine (AML) and hydrochlorothiazide (HCT) in human plasma was developed and validated. VAL, AML and HCT were resolved using a Gemini C 18 column and mobile phase gradient starting from 20 % acetonitrile and 80 % 10 mmol L -1 ammonium formate (V/V, pH 3.5 ± 0.2, by formic acid) to 70 % acetonitrile and 30 % 10 mmol L -1 ammonium formate, over 20 minutes, with a flow rate of 1 mL min -1. The samples were purified by protein precipitation and extraction. Telmisartan was used as internal standard. The method was validated according to USFDA and EMEA guidelines with good reproducibility and linear responses R = 0.9985 (VAL), 0.9964 (AML), and 0.9971 (HCT). RSDs of intra-and inter-day precision ranged 2.2-8.1 and 4.6-11.7 %, respectively, for all three drugs. Mean extraction recoveries of three QCs for the triple drug combination were 76.5 (VAL), 72.0 (AML) and 73.0 (HCT) % for human plasma. Although the LC-MS/MS method is more sensitive than HPLC, HPLC is still suitable for preliminary pharmacokinetic study. The experiments performed demostrated that simultaneous determination of all components of the triple drug combination in human plasma can be done by this method. Proposed method can be also used for guidance to the LC-MS/MS method.
Patel D.,Ganpat University |
Patel N.,Ganpat University
Acta Pharmaceutica | Year: 2010
Reversed-phase high-performance liquid chromatography (RP-HPLC) and thin-layer chromatography (TLC) methods have been developed and validated for simultaneous estimation of tamsulosin hydrochloride and finasteride in bulk drug and in combined dosage forms. RP-HPLC separation was achieved on a Phenomenex C 18 co lumn using methanol/0.02 mol L -1 ammonium acetate buffer/triethylamine (79.9 + 20 + 0.1, V/V/V) (pH 9.2) as mobile phase. TLC separation was achieved on an aluminium-backed layer of silica gel 60 F254 using toluene/methanol/triethylamine (9 + 1.5 + 1, V/V/V) as eluent. Quantification was achieved with photodiode array (PDA) detection at 235 nm over the concentration range 0.5-16 and 1-50 μg mL -1 with mean recovery of 99.8 ± 0.9 and 100.0 ± 0.8 % for tamsulosin hydrochloride and finasteride, respectively, by the RP-HPLC method. Quantification was achieved with UV detection at 270 nm over the concentration range 100-2000 ng per spot and 250-5000 ng per spot with mean recovery of 98.9 ± 0.9 and 99.6 ± 0.7 % for tamsulosin hydrochloride and finasteride, respectively, by the TLC method. Both methods are simple, precise, accurate and sensitive and are applicable to the simultaneous determination of tamsulosin hydrochloride and finasteride in bulk drug and in combined dosage forms.
Korac R.R.,University of Belgrade |
Khambholja K.M.,Ganpat University
Pharmacognosy Reviews | Year: 2011
Herbs have been used in medicines and cosmetics from centuries. Their potential to treat different skin diseases, to adorn and improve the skin appearance is well-known. As ultraviolet (UV) radiation can cause sunburns, wrinkles, lower immunity against infections, premature aging, and cancer, there is permanent need for protection from UV radiation and prevention from their side effects. Herbs and herbal preparations have a high potential due to their antioxidant activity, primarily. Antioxidants such as vitamins (vitamin C, vitamin E), flavonoids, and phenolic acids play the main role in fighting against free radical species that are the main cause of numerous negative skin changes. Although isolated plant compounds have a high potential in protection of the skin, whole herbs extracts showed better potential due to their complex composition. Many studies showed that green and black tea (polyphenols) ameliorate adverse skin reactions following UV exposure. The gel from aloe is believed to stimulate skin and assist in new cell growth. Spectrophotometer testing indicates that as a concentrated extract of Krameria triandra it absorbs 25 to 30% of the amount of UV radiation typically absorbed by octyl methoxycinnamate. Sesame oil resists 30% of UV rays, while coconut, peanut, olive, and cottonseed oils block out about 20%. A "sclerojuglonic" compound which is forming from naphthoquinone and keratin is the reaction product that provides UV protection. Traditional use of plant in medication or beautification is the basis for researches and making new trends in cosmetics. This review covers all essential aspects of potential of herbs as radioprotective agents and its future prospects.
Soni P.,Ganpat University
Proceedings of the IEEE | Year: 2010
M-commerce is the next generation of today's PC-based wired, e-commerce technology. The evolving success of mobile communication is transforming e-commerce into m-commerce. Users as a group of mobile devices are considered as a large potential market. Mobile commerce is any transaction involving the transfer of ownership or rights to use goods and services which is initiated and/or completed by using mobile access to computer-mediated networks with the help of an electronic device. Things are more complex when one or more operators are involved. There can be the case where the consumer and the merchant have accounts in different banks. Credit card or debit card information is stored in the mobile phone, so hackers can interpret it and use it for malicious purposes. The mobile operator will ask the consumer for his mobile personal identification number (MPIN). After verification of the MPIN, the mobile operator will verify the consumer's bank information.
Jadeja Y.,Ganpat University |
Modi K.,Ganpat University
2012 International Conference on Computing, Electronics and Electrical Technologies, ICCEET 2012 | Year: 2012
With the advent internet in the 1990s to the present day facilities of ubiquitous computing, the internet has changed the computing world in a drastic way. It has traveled from the concept of parallel computing to distributed computing to grid computing and recently to cloud computing. Although the idea of cloud computing has been around for quite some time, it is an emerging field of computer science. Cloud computing can be defined as a computing environment where computing needs by one party can be outsourced to another party and when need be arise to use the computing power or resources like database or emails, they can access them via internet. Cloud computing is a recent trend in IT that moves computing and data away from desktop and portable PCs into large data centers. The main advantage of cloud computing is that customers do not have to pay for infrastructure, its installation, required man power to handle such infrastructure and maintenance. In this paper we will discuss what makes all this possible, what is the architectural design of cloud computing and its applications. © 2012 IEEE.
Sharma R.N.,Ganpat University |
Ravani R.,Ganpat University
Medicinal Chemistry Research | Year: 2013
Synthesis of novel quinazolinone derivatives was performed from the reaction of N-benzoyl substituted piperazine-1-carbothioamide with 2-chloromethyl quinazolinone derivatives and screened for their in vitro cytotoxic activity by MTT assay. The cell lines used were NCI (human lung cancer cell), MCF 7 (Breast cancer cell), and HEK 293 (Normal epidermal kidney cell). Result of screening on cell line showed moderate to good anticancer activity for all the compounds. Compound 3d (IC50 = 1.1 ± 0.03 μM) was found to be the most active compared to standard methotrexate (IC50 = 2.20 ± 0.18 μM) and 5-florouracil (IC50 = 2.30 ± 0.49 μM). Structure activity relationship of synthesized analogs suggested that the presence of NH linker with aryl moiety at the third position of quinazolinone ring was important for potent anticancer activity. Electron donating group on phenyl ring at the third position of quinazolinone ring gave better anticancer activity then unsubstituted phenyl and electron withdrawing group. Activity by substituted piperazine at 2nd position of thiazole linked with quinazolinone scaffold gave better activity in the order of H > CH 3 > CO-C6H5. Our findings may impart new direction to medicinal chemists and biochemists for further investigations of quinazolinone-thiazole containing anticancer agents. © 2012 Springer Science+Business Media New York.
Sharma R.,Ganpat University |
Pancholi S.,Ganpat University
Acta Pharmaceutica | Year: 2010
A simple, sensitive and precise RP-HPLC-DAD method was developed and validated for the determination of olmesartan medoxomil (AT-II receptor blocker) in the presence of its degradation products. Olmesartan medoxomil and all the degradation products were resolved on a C18 column with the mobile phase composed of methanol, acetonitrile and water (60:15:25, V/V/V, pH 3.5 by orthophosphoric acid) at 260 nm using a photodiode array detector. The method was linear over the concentration range of 1-18 μg mL-1 and precise with RSD < 1 % in intra- and inter-day study. Excellent recoveries of 99.3 ± 0.9 to 100.8 ± 1.2% proved the accuracy of the method. Developed method was specific, as indicated by chromatographic resolution > 2.0 for each peak and sensitive with LOD 0.03 μg mL-1 and LOQ 0.1 μg mL-1. The method was used to study the drug degradation behavior under forced conditions. Four degradation products (DP-I, II, III, IV) were formed during the degradation study in 0.1 mol L-1 HCl whereas only DP-I, II and III were formed in water, 0.01 mol L-1 NaOH and 3% H2O2. No significant thermal or photolytic degradation was observed in solid drug. The method was applied successfully for the assay of olmesartan medoxomil in the tablet dosage form.