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Luo R.,CAS Shanghai Institute of Organic Chemistry | Luo R.,Gannan Medical University | Li K.,CAS Shanghai Institute of Organic Chemistry | Hu Y.,CAS Shanghai Institute of Organic Chemistry | Tang W.,CAS Shanghai Institute of Organic Chemistry
Advanced Synthesis and Catalysis | Year: 2013

A new C2-symmetrical, chiral bisphosphorus ligand proved to be efficient for the rhodium-catalyzed nucleophilic addition of arylboronic acids to trifluoromethyl ketones, providing a series of chiral trifluoromethyl- substituted tertiary alcohols in high yields (up to 93%) and excellent enantioselectivities (>99%). Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Liu L.,Wuhan University | Chen X.,Wuhan University | Xie S.,Gannan Medical University | Zhang C.,Wuhan Medical Care Center for Women and Children | And 2 more authors.
Hepatology | Year: 2012

KIAA0101 overexpression was detected in numerous malignant solid tumors and involved in tumor progression; however, the correlation between KIAA0101 expression level and human hepatocellular carcinoma (HCC) was controversial. Our data revealed abnormal expression of the KIAA0101 transcript variant 1 (KIAA0101 tv1) at both messenger RNA and protein levels in HCC tissues and cell lines assessed by semiquantitative reverse-transcription polymerase chain reaction (RT-PCR), virtual northern blot, western blot, and immunohistochemical analysis, especially in stage 3-4 HCCs. NIH3T3 cells transfected with KIAA0101 tv1 induced colony formation in vitro and tumor xenorafts in vivo, implying the oncogenic potential of KIAA0101 tv1. Semiquantitative RT-PCR, real-time quantitative RT-PCR, and western blot analysis demonstrated that doxorubicin (Adriamycin, ADR) treatment down-regulated expression of the KIAA0101 tv1, whereas it increased the acetylation of the p53 protein. Additionally, KIAA0101 tv1 prevented cells from apoptosis caused by ADR through suppressing the acetylation of p53 at Lys382. Immunoprecipitation analysis and mammalian two-hybrid assay indicated that KIAA0101 tv1 bound to the transactivation region (1-42 amino acids) of p53 and strongly inhibits its transcriptional activity. Taken together, our data suggest that KIAA0101 tv1 played an important role in the late stage of metastatic HCC and prevented apoptosis after chemotherapeutic drug treatment through inhibiting the transcriptional activity of the p53 gene. Conclusion: KIAA0101 tv1 may function as a regulator, promoting cell survival in HCC through regulating the function of p53. Suppression of the KIAA0101 tv1 function is likely to be a promising strategy to develop novel cancer therapeutic drugs. © 2012 American Association for the Study of Liver Diseases.


Chen L.,Gannan Medical University
International Journal of Multimedia and Ubiquitous Engineering | Year: 2016

Airway suffocation is a kind of unexpected emergency. The author's research group proposed a new spot-first-aid method of foreign body asphyxia in the hypopharynx, and compares it with traditional “Heimlich Maneuver” first aid method by simulation comparative study. This article mainly introduced the main contents and methods of the research, primarily on: the processing of CT Imaging data, including: Visual C++ program is used to realize the contour extraction algorithm processing based on CT images pre-processing, and restore the respiratory organs in asphyxia status in the processing of image morphology. Compare the two spot first aid methods by simulation. The Main research steps including: establishing simulated equivalent circuit simulation to compare two different spot first aid methods influence on the change of respiratory airway pressure and the animals steps of the experiments. © 2016 SERSC.


Shi J.,CAS Shanghai Institute of Materia Medica | Yan Y.,CAS Shanghai Institute of Materia Medica | Yan Y.,Gannan Medical University | Li Q.,CAS Shanghai Institute of Materia Medica | And 3 more authors.
Chemical Communications | Year: 2014

Here a new, mild and versatile method for efficient synthesis of a diverse range of 2-acetate substituted indoles via Rh(iii)-catalyzed and alcohol-mediated C2-selective carbenoid insertion functionalization of indoles by α-diazotized Meldrum's acid has been developed. Furthermore, for the first time, a Rh(iii)/Cu(ii)-catalyzed direct C7-alkenylation of such functionalized products has also been demonstrated. This journal is © the Partner Organisations 2014.


Jing Z.,Gannan Medical University
Advances in Experimental Medicine and Biology | Year: 2010

Objective: To research the 3'-daidzein sulfonic sodium's (DSS) effect on antioxidation of retinal ischemia/reperfusion (RI/R) injury in rats. Methods: RI/R in rats was made by reperfusion for 1 h after occlusion of common carotid artery (CCA) for 1 h and the rats were divided into four groups randomly, including sham control group, model group of RI/R injury, low-dosage DSS group and high-dosage DSS group. 1 ml/kg NS was injected through sublingual vein after CCA was dissociated in sham group. Other groups were treated with normal saline, low-dosage DSS (1 mg/kg) and high-dosage DSS (2 mg/kg) through sublingual vein respectively before occlusion of CCA. After occlusion of CCA for 1 h and reperfusion for 1 h, blood was put out and separated into the serum. Then detect the contents of MDA and NO, the activity of SOD and GSH-PX. Result: (1) The content of MDA was increased in model group (P<0.05), and lowdosage DSS can reverse it (P<0.05). (2) The content of serum NO in model group was lower than in sham group (P<0.001). The content of serum NO in model group is lower than in low-dosage DSS group (P<0.05) and high-dosage DSS group (P<0.01) respectively. (3) The activity of GSH-PX in high-dosage group was higher than in model group (P<0.05). (4) The activity of SOD in low-dosage group was higher than in model group (P<0.05). Conclusion: DSS can decrease the cellular damage and protect the optical function in RI/R injury through elevating the content of NO, the activity of SOD and GSH-PX, and increasing anti-oxidation. © Springer Science+Business Media, LLC 2010.

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