Ping S.,Gannan Medical College
Journal of Central South University (Medical Sciences) | Year: 2016
Objective: To detect the expressions of human epidermal growth factor receptor 2 (HER2) and Topo IIα in breast cancer, and to analyze the clinical significance of neoadjuvant chemotherapy for the anthracycline-based drugs. Methods: The HER2 and Topo Ila gene and protein expressions in cancer tissues from 189 patients with breast cancer were detected by fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC). And the objective response rate (ORR) and pathological complete rate (PCR) were analyzed. Results: The HER2 protein expression in 46 patients (24.3%) and Topo Ila protein expression in 55 patients (29.1%) was 3+ by IHC or they were 49 (25.9%) and 94 (49.0%) by FISH, respectively. The ORR and PCR in HER2 negative or positive patients were 47.4% and 20.3% or 32.7% and 16.3%, respectively, with significant differences (All P<0.05). The ORR and PCR in Topo Ila positive or negative patients were 69.1% and 36.0% or 28.4% and 2.2%, respectively, with significant differences (All P<0.05). Conclusion: FISH and IHC were consistent in the determination of HER2 expression whereas they were inconsistent in the determination of Topo IIα expression. The amplification of Topo Ila can effectively improve the effect of the adjuvant treatment effect of the anthracyclines.
Xiong J.,Gannan Medical College |
Song T.,Teaching and Research Section of Biochemistry and Molecular Biology
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010
BACKGROUND: With development of modern science, various high energy rays, which are widely applied for military affairs, communication, medical science, and daily life, directly cause increasing of radiation dose annually. Therefore, image quality and radiation protection attract more and more attention. Radiation protection plays an important role in modern medical science. For protecting human health, protection tools are necessary for shielding varying rays and microwaves. OBJECTIVE: To summarize mechanism, classification, characteristics, and research status of radiation protection materials. METHODS: Articles were retrieved from PubMed database (http://www.ncbi.nlm.nih.gov/ PubMed) and Wanfang database (http://www.wanfangdata.com.cn) with the key words of "shielding materials, shielding mechanism, protection, classification" from 1982 to 2010. Inclusion criteria: Articles related to shielding mechanism, classification, characteristics, and research status were included. Duplicated papers were excluded. Among 23 articles, 16 ones were included in the final analysis. RESULTS AND CONCLUSION: Shielding materials absorbed rays depending on energy absorption and particle absorption. Medical ray protection materials included organic glass, compound protection materials mixed by a certain ratio of lead, wolfram, and barium, and varying radiation protection clothing. With the development of science and technology, adverse effects induced by various radiations should be relieved, so protection materials and techniques need to be further studied for clinical application.
Mao X.,Sun Yat Sen University |
Yin W.,Sun Yat Sen University |
Liu M.,Sun Yat Sen University |
Ye M.,Sun Yat Sen University |
And 5 more authors.
Brain Research | Year: 2011
Previously we demonstrated that Osthole, a natural coumarin, protects against focal cerebral ischemia/reperfusion-induced injury in rats. In the present study, the effects of Osthole on neurobehavioral functions, infarct volume and matrix metalloproteinase-9 (MMP-9) in a rat 2 h focal cerebral ischemia model were investigated. Osthole (100 mg/kg per dose) was administrated intraperitoneally 30 min before ischemic insult and immediately after reperfusion. Osthole treatment significantly reduced neurological deficit score and infarct volume by 38.5% and 33.8%, respectively, as compared with the untreated animals. Osthole reversed ischemia-reperfusion-induced increase in MMP-9 protein level/activity as evidenced by Western blotting and gelatin zymography. Taken together, these results for the first time demonstrate that Osthole reduces infarct volume, restores neurobehavioral functions and downregulates MMP-9 protein level/activity in ischemia/reperfused brain. © 2011 Elsevier B.V.
Xiang X.J.,Nanchang University |
Liu Y.W.,Nanchang University |
Zhang L.,Nanchang University |
Qiu F.,Nanchang University |
And 6 more authors.
Anti-Cancer Drugs | Year: 2012
This study aimed at assessing the efficacy and safety of biweekly oxaliplatin in combination with continuous infusional 5-fluorouracil and leucovorin (modified FOLFOX regimen) in patients with advanced small bowel adenocarcinoma (SBA). Thirty-three eligible patients with previously untreated SBA received 85 mg/m of oxaliplatin intravenously over a 2-h period on day 1, together with 400 mg/m of leucovorin over 2 h, followed by a 46-h infusion of 5-FU 2600mg/m every 2 weeks. All patients were evaluable for efficacy and toxicity. A median of nine cycles (range 3-18) was administered. The objective response rate was 48.5% [95% confidence interval (95% CI): 31-67%], with one complete response, 15 partial responses, 12 stable diseases, and five progressions. The median time to progression was 7.8 months (95% CI: 6.0-9.6) and the median overall survival was 15.2 months (95% CI: 11.0-19.4). Toxicity was fairly mild. Grade 3 toxicities included neutropenia (12.1%), thrombocytopenia (3.0%), nausea (6.1%), vomiting (3.0%), diarrhea (3.0%), peripheral neuropathy (9.1%), and fatigue (3.0%), and grade 4 toxicities occurred in none of the patients. The modified FOLFOX regimen is highly active and well tolerated as first-line chemotherapy for advanced SBA patients. © 2012 Lippincott Williams & Wilkins, Inc.
Shumei L.,Gannan Medical College
Advances in Experimental Medicine and Biology | Year: 2010
Objective: Mutations in the inosine monophosphate dehydrogenase 1 gene (IMPDH1) have recently been discovered that IMPDH1 gene plays a critical role in pathogenesis of autosomal dominant retinitis pigmentosa (adRP). Aiming towards an understanding of the molecular background of retinitis pigmentosa (RP), this paper investigates the mutation frequency of IMPDH1 genes in the Han patients with adRP in Ganzhou City. Methods: The whole blood samples were collected randomly from 56 adRP patients and 62 unrelated normal controls who were residents of Han population in Ganzhou City, and then their genomic DNA samples were extracted respectively. Genic polymorphism was examined by the polymerase chain reaction and restrictionfragment-length polymorphisms (PCR-RFLP). The statistical significance of the data was further analyzed by SPSS 14.0 software. Results: Mutation rate of IMPDH1 gene had no significance between in adRP patients and in the normal control by exact probabilities in 2 × 2 table (p = 0.232). The mutation frequency of IMPDH1gene in the Han samples was 3.6%. Conclusion: The mutation frequency of IMPDH1 gene of the Han population in Ganzhou city was similar as approximately 2-5% of the adRP cases among Americans of European origin and Europeans. © Springer Science+Business Media, LLC 2010.
He W.,Gannan Medical College |
Chen W.,Gannan Medical College |
Zhou Y.,Gannan Medical College |
Tian Y.,Gannan Medical College |
Liao F.,Gannan Medical College
Cellular and Molecular Neurobiology | Year: 2013
We previously found that xanthotoxol, one of the major active ingredients in Cnidium monnieri (L.) Cusson, exerts protective effects in a rat model of focal cerebral ischemia/reperfusion injury by alleviating brain edema, inhibiting the neutrophil infiltration, and decreasing the expression of intercellular adhesion molecule-1 (ICAM-1) and E-selectin. The present study was designed to further determine the possible mechanisms of action of neuroprotective properties of xanthotoxol after cerebral ischemia. Transient focal cerebral ischemia/reperfusion model in male Sprague-Dawley rats was induced by 2-h middle cerebral artery occlusion followed by 24-h reperfusion. Xanthotoxol (5 and 10 mg/kg) or vehicle were administered intraperitoneally at 1 and 12 h after the onset of ischemia. At 24 h after reperfusion, we assessed the effect of xanthotoxol on the blood-brain barrier (BBB) permeability, the production of pro-inflammatory mediators such as interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-8, nitric oxide (NO), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and the p65 subunit of the transcription factor, nuclear factor-κB (NF-κB) in the cortex after ischemic insult. The results showed that xanthotoxol treatment significantly attenuated BBB disruption, reduced the IL-1β, TNF-α, IL-8 and NO level, and attenuated the iNOS activity compared with vehicle-treated animals. Further, xanthotoxol treatment also significantly prevented the ischemia/reperfusion-induced increase in the protein expression of iNOS, COX-2, and the nuclear NF-κB p65. These results, taken together with those of our previous study, suggest that the neuroprotection may be attributed to the ability of xanthotoxol to attenuate the expression of pro-inflammatory mediators and thereby inhibit the inflammatory response after cerebral ischemia. © 2013 Springer Science+Business Media New York.
He W.,Huazhong University of Science and Technology |
He W.,Gannan Medical College |
Xu X.,Huazhong University of Science and Technology |
Lv Q.,Huazhong University of Science and Technology |
Guo L.,Huazhong University of Science and Technology
Cellular and Molecular Neurobiology | Year: 2014
Focal cerebral ischemia can impair the induction of activity-dependent long-term potentiation (LTP) in the hippocampus. This impairment of hippocampal synaptic plasticity can be caused by excitotoxicity and subsequent perturbation of hippocampal LTP-relevant transmitter systems, which include NR2B and PSD-95. It has been suggested that hyperpolarization-activated cyclic-nucleotide-gated (HCN) channels may play an important role in the control of membrane excitability and rhythmic neuronal activity. Our previous study has indicated that the selective HCN channel blocker ZD7288 can produce a dose-dependent inhibition of the induction of LTP at the Schaffer collateral-CA1 synapse of hippocampus by reducing the amount of glutamate released. It has also been demonstrated that ZD7288 can protect against neuronal injury caused by oxygen glucose deprivation. In the present study, we investigated the effect of ZD7288 on the induction of activity-dependent LTP and the expression of NR2B and PSD-95 after focal cerebral ischemia/reperfusion injury. The results showed that the induction of LTP was significantly impaired and the levels of NR2B and PSD-95 mRNA and protein were markedly decreased in the CA1 region of hippocampus following focal cerebral ischemia/reperfusion injury. Administration of low dose ZD7288 (0.25 μg) at 30 min and 3 h after the onset of ischemia attenuated the impairment of LTP induction and alleviated the NR2B and PSD-95 mRNA and protein down-regulation commonly induced by cerebral ischemia/reperfusion injury. These results suggest that low dose ZD7288 can ameliorate the ischemia/reperfusion-induced impairment of synaptic plasticity in the hippocampal CA1 region. © 2014 Springer Science+Business Media New York.
Shumei L.,Gannan Medical College
Advances in Experimental Medicine and Biology | Year: 2010
Objective: To study the effect of hesperidin on expression of inducible nitric oxide synthase (iNOS) in cultured rabbit retinal pigment epithelial (RPE) cells under the condition of high glucose in vitro. Method: Hesperidin was extracted from Pericarpium Citri Reticulatae by ultrasound and ethanol precipitation and was detected qualitatively by high performance liquid chromatogram. The third to fifth primary cultured rabbit RPE were selected. The cells were divided into 6 groups including the control group cultured in DMEM, the model group cultured in DMEM containing 33 mmol/L glucose without any drug and four experimental groups which were exposed to hesperidin at the concentration of 10, 20, 40 and 80 mg/L at 37°C under 5% CO2 for 2 h and then cultured in DMEM containing 33 mmol/L glucose. The proliferation of RPE was measured by the MTT assay. The levels of NO produced were measured by spectrophotometry. The changes of iNOS expressed in RPE cells were determined with immunohistochemistry. Results: The growth rate of RPE cells was associated with the concentration of hesperidin. NO production induced by high glucose was significantly inhibited by hesperidin. iNOS expression in hesperidin-treated group was decreased compared with the control group (p <0.001). Conclusion: Hesperidin can increase the proliferation of rabbit RPE cells, and inhibit the level of NO and iNOS expression, so hesperidin can protect rabbit RPE cells. © Springer Science+Business Media, LLC 2010.
Zhong B.Y.,Gannan Medical College
Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery | Year: 2012
To explore the clinical value of 64-slice spiral 3-phase CT enhanced scanning for preoperative TNM staging assessment of gastric carcinoma. A retrospective study was performed to review the 64-slice spiral 3-phase CT enhanced scanning of 120 patients with gastric cancer diagnosed by biopsy prior to operation and postoperative pathological reports. All the findings were reviewed by two senior radiologic diagnosticians separately and compared with pathological findings. The accuracy of 64-slice spiral CT enhanced scan was 79.2%(95/120) for T staging, 66.7%(10/15) for T1, 66.7%(14/21) for T2, 84.0%(42/50) for T3, and 85.3%(29/34) for T4. For gastric wall with single layer and multiple layers, the accuracy of CT enhanced scanning was 59.4%(19/32) and 81.8%(72/88) for T staging, and the difference was statistically significant(P<0.05). The accuracy of 64-slice spiral CT enhanced scan was 73.9%(85/115) for N staging, 75.5%(37/49) for N0, 70.3%(26/37) for N1, 75.9%(22/29) for N2. The accuracy of 64-slice spiral CT enhanced scanning was 89.2% for M staging. 64-slice spiral CT 3-phase enhanced scanning can monitor the invasion, lymphatic metastasis, and distant metastasis of gastric cancer dynamically, which may become an important examination item for the preoperative evaluation of gastric cancer.
PubMed | Gannan Medical College
Type: Journal Article | Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences | Year: 2016
To detect the expressions of human epidermal growth factor receptor 2 (HER2) and Topo II in breast cancer, and to analyze the clinical significance of neoadjuvant chemotherapy for the anthracycline-based drugs. Methods: The HER2 and Topo II gene and protein expressions in cancer tissues from 189 patients with breast cancer were detected by fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC). And the objective response rate (ORR) and pathological complete rate (pCR) were analyzed. Results: The HER2 protein expression in 46 patients (24.3%) and Topo II protein expression in 55 patients (29.1%) was 3+ by IHC or they were 49 (25.9%) and 94 (49.0%) by FISH, respectively. The ORR and pCR in HER2 negative or positive patients were 47.4% and 20.3% or 32.7% and 16.3%, respectively, with significant differences (All P<0.05). The ORR and pCR in Topo II positive or negative patients were 69.1% and 36.0% or 28.4% and 2.2%, respectively, with significant differences (All P<0.05). Conclusion: FISH and IHC were consistent in the determination of HER2 expression whereas they were inconsistent in the determination of Topo II expression. The amplification of Topo II can effectively improve the effect of the adjuvant treatment effect of the anthracyclines.