Gangwon Agricultural Research and Extension Services

Hongch’ŏn, South Korea

Gangwon Agricultural Research and Extension Services

Hongch’ŏn, South Korea
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Han K.,National Institute of Agricultural Science | Han S.,Hanyang University | Kim E.,Foundation of Agricultural Technology Commercialization and Transfer | Cho H.,National Institute of Agricultural Science | And 6 more authors.
Applied Biological Chemistry | Year: 2017

Excess nutrients are easily accumulated in greenhouse soils due to the interception of rainfall by plastic film and repeated over-application of compost and fertilizers. This study was conducted to evaluate the application of electrokinetic (E/K) technology for salt removal from soils with high electrical conductivity (EC) in greenhouses. Three types of soil in plastic film greenhouses were used: artificial soil (Site A), poorly drained soil (Site B), and well-drained soil (Site C). The salt-enriched surface soils were used to fill 37-cm-long-box-type E/K cells, and a constant voltage gradient was applied at a rate of 1 V cm−1 for 30 days. The decrease in soil EC was achieved with water content of greater than 30% for silt loam (Sites A and B) and 20% for sandy loam (Site C). The E/K technology decreased soil EC by more than 80%, with a greater reduction ratio for sandy loam than for silt loam. After 30 days of the E/K treatment, 98–99% of NO3-N and 95–99% of sodium were removed in all three sites under saturated condition, implying that nitrate and sodium ions had higher mobility than the other ions during the E/K process. The results obtained from the study suggested, therefore, that the E/K technology is highly efficient for wet and nitrate-enriched sandy loam soils, and the technology can be a feasible and environmentally sound practice for the removal of excessive nutrients in greenhouse soils without water pollution by nutrients such as nitrate as can be caused by flooding and repeated washing with water. © 2017 The Korean Society for Applied Biological Chemistry


Lee K.J.,Gangwon Agricultural Research and Extension Services | Yun I.J.,Gangwon Agricultural Research and Extension Services | Kim K.H.,Gangwon Agricultural Research and Extension Services | Lim S.H.,Gangwon Agricultural Research and Extension Services | And 3 more authors.
Journal of Food Composition and Analysis | Year: 2011

The amino acid profiles and fatty acid composition of Agrocybe chaxingu were studied to evaluate the nutritional value of this species. The most abundant essential amino acid and non-essential amino acid were leucine and glutamate, respectively. The fruiting body of A. chaxingu contained 0.61. g leucine/100. g of edible weight, while those of Pleurotus ostreatus and Flammulina velutipes contained 0.53. g and 0.38. g leucine/100. g of edible weight, respectively. The content of glutamate ranged from 1.00 to 1.20. g/100. g of edible weight. Total amount of the essential amino acids of A. chaxingu (2.70. g/100. g of edible weight) was higher than those of the P. ostreatus and F. velutipes used in this study. Linoleic acid, a polyunsaturated fatty acid, was the major component in A. chaxingu (69.3% of total fatty acids), and the second highest component of fatty acids was palmitic acid (13.7%). The amount of MUFA (monounsaturated fatty acid) and PUFA (polyunsaturated fatty acid) in the fruiting body of A. chaxingu was 5.0% and 69.3% of the total fatty acid content, respectively. © 2010 Elsevier Inc.


Sa K.J.,Kangwon National University | Park J.Y.,Kangwon National University | Park J.Y.,Gangwon Agricultural Research and Extension Services | Woo S.Y.,Kangwon National University | And 3 more authors.
Genes and Genomics | Year: 2015

In this study, 80 F7:8 recombinant inbred lines, derived from a cross between dent corn and waxy corn, were evaluated for ten grain yield and kernel quality-related traits over a 2-year period. A total of 39 quantitative trait loci (QTLs) and 74 epistatic interactions were confirmed in 2011 and 2012. All QTLs detected in 2011 showed greater than 10 % phenotypic variation (10.21–50.3 %). In 2012, 19 of 23 QTLs showed phenotypic variation greater than 10 % (6.46–93.71 %). Among all QTLs detected, qAC9 had the highest phenotypic variance (93.71 %). Additionally, qEH4, qSEL6, and q100KW10 were stably expressed. These QTLs had higher phenotypic variance and were observed in both years; therefore, they may be considered major QTLs. Some new QTLs identified in this study were located on different loci compared with other studies. The genetic region (bin 4.08) strongly controls plant height and ear height, and results from pleiotropy and/or tight linkage. qST3 and qEH3 were co-located within two common adjacent simple sequence repeat (SSR) markers (umc2275 and umc1273), whereas qEL6 and qSEL6 were co-located within two common adjacent SSR markers (umc2309 and bnlg238). The QTLs related to kernel quality traits, qWC4 (water content) was tightly linked to the gene bt2 (brittle endosperm 2) and qAC9 (amylose content) located between the flanking markers umc1634 and wx1 (waxy1). Thus, these SSR markers are a useful selection tool for screening grain yield and yield component traits. © 2014, The Genetics Society of Korea and Springer-Science and Media.


Park J.Y.,Kangwon National University | Park J.Y.,Gangwon Agricultural Research and Extension Services | Ramekar R.V.,Kangwon National University | Sa K.J.,Kangwon National University | Lee J.K.,Kangwon National University
Genes and Genomics | Year: 2015

Assessing genetic diversity, population structure, and linkage disequilibrium is important in identifying potential parental lines for breeding programs. In this study, we assessed the genetic and phenotypic variation of 174 normal maize (Zea mays) inbred lines and made association analyses with respect to nine agronomical traits, using 150 simple sequence repeats (SSR). From population structure analysis, the lines were divided into three groups. Association analysis was done with a mixed linear model and a general linear model. Twenty-one marker-trait associations involving 19 SSR markers were observed using the mixed model, with a significance level of P < 0.01. All of these associations, as well as 120 additional marker-trait associations involving 77 SSR markers, were observed with the general model. Two significant marker-trait associations (SMTAs) were detected at P ≤ 0.0001. In the mixed linear model, one locus was associated with water content, two loci were associated with 100-kernel weight, setted ear length, ear thickness and stem thickness; three loci were associated with ear height, four loci were associated with total kernel weight and five loci were associated with plant height. These results should prove useful to breeders in the selection of parental lines and markers. © 2015, The Genetics Society of Korea and Springer-Science and Media.


Kang K.,Korea Institute of Science and Technology | Kang K.,Seoul National University | Jho E.H.,Korea Institute of Science and Technology | Lee H.J.,Korea Institute of Science and Technology | And 8 more authors.
Journal of Medicinal Food | Year: 2011

Improvement of liver function is one of the most popular commercial health claims of functional foods in Asian countries, including Korea. After examining the potential of several traditional Korean wild vegetables for enhancing liver function, we found that Youngia denticulata Kitam. has strong hepatoprotective effects against oxidative stress induced by tert-butylhydroperoxide (t-BHP). We are the first to report that the extract and ethyl acetate fractions of Y. denticulata have radical scavenging activities and inhibit oxidative stress-induced cell death and DNA damage in HepG2 cells. The extract and ethyl acetate fractions significantly decreased cellular reactive oxygen species production and apoptosis induced by t-BHP in HepG2 cells. In addition, they prevented the depletion of cellular glutathione, which is an important defense molecule against oxidizing xenobiotics. Chlorogenic acid and 3,5-dicaffeoylquinic acid were found to be major active components responsible for the activity of Y. denticulata and could serve as marker compounds for standardization. These data suggest that Y. denticulata could be promoted as a potential antioxidative functional food candidate, particularly for hepatoprotection against oxidative stress. © 2011 Mary Ann Liebert, Inc.


Sa K.J.,Kangwon National University | Park J.Y.,Gangwon Agricultural Research and Extension Services | Park K.-C.,Kangwon National University | Lee J.K.,Kangwon National University
Genes and Genomics | Year: 2012

A maize genetic linkage map was generated using SSR and SNP markers in a F7:8 recombinant inbred line (RIL) population derived from a cross of waxy corn (KW7) and dent corn (Mo17). A total of 465 markers, including 459 SSR and 6 SNP markers, were assigned to 10 linkage groups which spanned 2,656.5 cM with an average genetic distance between markers of 5.7 cM, and the number of loci per linkage group ranged from 39 to 55. The SSR (85.4%) and SNP (83.3%) markers showed Mendelian segregation ratios in the RIL population at a 5% significance threshold. In linkage analysis of six SNP loci associated with kernel starch synthesis genes (ae1, bt2, sh1, sh2, su1, and wx1), all six loci were successfully mapped and are closely linked with SSR markers in chromosomes 3 (sh2), 4 (su1 and bt2), 5 (ae1), and 9 (sh1 and wx1). The SSR markers linked with genes in starch synthesis may be utilized in marker assisted breeding programs. The resulting genetic map will be useful in dissection of quantitative traits and the identification of superior QTLs from the waxy hybrid corn. Additionally, these data support further genetic analysis and development of maize breeding programs. © The Genetics Society of Korea and Springer 2012.


Sa K.J.,Kangwon National University | Park J.Y.,Kangwon National University | Park J.Y.,Gangwon Agricultural Research and Extension Services | Choi S.H.,Kangwon National University | And 3 more authors.
Genetics and Molecular Research | Year: 2015

Understanding genetic diversity, population structure, and linkage disequilibrium is a prerequisite for the association mapping of complex traits in a target population. In this study, the genetic diversity and population structure of 40 waxy and 40 normal inbred maize lines were investigated using 10 morphological traits and 200 simple sequence repeat (SSR) markers. Based on a population structure analysis, the 80 maize inbred lines were divided into three groups: I, II, and admixed. Significant marker-trait associations were identified between the markers and the 10 morphological traits, which were studied according to the model used to confirm the association. Using a general linear model, the lowest R2 value (9.03) was detected in umc1139, which was associated with ear number, and the highest (43.97) was in umc1858, which was associated with plant height. Using a mixed linear model, the lowest R2 value (18.74) was in umc1279, which was associated with ear weight; the highest (27.66) was in umc1858, which was associated with 100-kernel weight. The SSR markers identified in the present study may serve as useful molecular markers for selecting important yield and agronomic traits. These results will be useful for marker-assisted selection in maize breeding programs, to help breeders choose parental lines and markers for crosses. © FUNPEC-RP.


Jin S.K.,Kangwon National University | Kyong-Cheul P.,Kangwon National University | Vasudeo R.R.,Kangwon National University | Yeol P.J.,Gangwon Agricultural Research and Extension Services | Kyong L.J.,Kangwon National University
Research Journal of Biotechnology | Year: 2016

In this study, a total of 80 F7:8 recombinant inbred lines (RIL), derived from a cross between dent corn inbred line, Mo17 and waxy inbred line KW7, were evaluated for 11 flowering and yield-related traits. Correlation analysis was performed among the 11 flowering and yield-related traits. DA showed the highest correlation with DS (0.747∗∗). DS via ASI (0.619∗∗), TC via SC (0.595∗∗), CB via ER (0.535∗∗) showed relatively high correlation coefficients than the other trait combinations. Quantitative trait loci (QTL) and epistatic interactions were detected for 11 flowering and yield-related traits. Our study detected a total of 32 QTLs and 40 epistatic interactions for 11 flowering and yieldrelated traits. The 15 QTLs were major QTLs which had more than 15% phenotypic variation (16.29~82.45%). qKT9 had the highest phenotypic variance (82.45%) in marker interval between umc1634 and wx1. The qDS1a and qASI1a were colocated within a region flanked by two adjacent SSR markers on chromosome 1 (umc1354 and bnlg1179) and qDS1b and qASI1b were co-located within a region flanked by two adjacent SSR markers on chromosome 1 (umc1166 and umc1976). In addition, two QTLs, qCB3 and qDA3 shared umc1949 and Two QTL, q10V5 and qDS5 also shared umc2373. Another QTL, qER10 and qASI10 shared umc1645. This tight link supported high correlation of each trait. Finally, these SSR marker pairs are useful selection tools for screening flowering and yield related traits. To perform MAS efficiently, it is important to identify genes or traits on the chromosomal segment linked to the target gene. Thus, the detection and confirmation of loci associated with main agronomic traits presented may provide greater opportunities for maize breeders to improve quality with marker associated selection.


PubMed | Gangwon Agricultural Research and Extension Services and Kangwon National University
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

Understanding genetic diversity, population structure, and linkage disequilibrium is a prerequisite for the association mapping of complex traits in a target population. In this study, the genetic diversity and population structure of 40 waxy and 40 normal inbred maize lines were investigated using 10 morphological traits and 200 simple sequence repeat (SSR) markers. Based on a population structure analysis, the 80 maize inbred lines were divided into three groups: I, II, and admixed. Significant marker-trait associations were identified between the markers and the 10 morphological traits, which were studied according to the model used to confirm the association. Using a general linear model, the lowest R(2) value (9.03) was detected in umc1139, which was associated with ear number, and the highest (43.97) was in umc1858, which was associated with plant height. Using a mixed linear model, the lowest R(2) value (18.74) was in umc1279, which was associated with ear weight; the highest (27.66) was in umc1858, which was associated with 100-kernel weight. The SSR markers identified in the present study may serve as useful molecular markers for selecting important yield and agronomic traits. These results will be useful for marker-assisted selection in maize breeding programs, to help breeders choose parental lines and markers for crosses.

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