Gangneung Asan Hospital

Kang-neung, South Korea

Gangneung Asan Hospital

Kang-neung, South Korea
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Li H.,Korea Institute of Science and Technology | Li H.,Korean University of Science and Technology | Li H.,Qiqihar University | Jang W.,University of Ulsan | And 8 more authors.
NeuroReport | Year: 2015

In our previous study, the neuroprotective effect of calcitriol was confirmed in SH-SY5Y cells. In this article, we explored whether calcitriol showed neuroprotection in a subchronic 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)- treated mouse model of Parkinson's disease. After mice were treated with MPTP alone or cotreated with MPTP and calcitriol, the substantia nigra pars compacta was dissected, and related protein levels were detected by western blot. Our results suggest that the MPTP-injected mice treated with calcitriol had attenuated tyrosine hydroxylase expression and increased LC3-II conversion compared with those that were not. Above all, calcitriol showed neuroprotection in the MPTP mouse model. © 2015 Wolters Kluwer Health, Inc.


Jang W.,University of Ulsan | Jang W.,Gangneung Asan Hospital | Kim H.J.,Korea Institute of Science and Technology | Kim H.J.,Gangneung Asan Hospital | And 4 more authors.
Molecular Neurobiology | Year: 2016

Currently, the autophagy pathway is thought to be important for the pathogenesis of Parkinson’s disease (PD), and the modulation of autophagy may be a novel strategy for the treatment of this disease. Erythropoietin (EPO) has been reported to have neuroprotective effects through anti-oxidative, anti-apoptotic, and anti-inflammatory mechanisms, and it has also been shown to modulate autophagy signaling in an oxygen toxicity model. Therefore, we investigated the effects of EPO on autophagy markers and evaluated its neuroprotective effect on rotenone-induced neurotoxicity. We adapted the rotenone-induced neurotoxicity model to SH-SY5Y cells as an in vitro model of PD. We measured cell viability using MTT and annexin V/propidium iodide assays and measured intracellular levels of reactive oxygen species. Immunofluorescence analysis was performed to measure the expression of LC3 and α-synuclein. Intracellular signaling proteins associated with autophagy were examined by immunoblot analysis. EPO mono-treatment increased the levels of mammalian target of rapamycin (mTOR)-independent/upstream autophagy markers, including Beclin-1, AMPK, and ULK-1. Rotenone treatment of SH-SY5Y cells reduced their viability, increased reactive oxygen species levels, and induced apoptosis and α-synuclein expression, and simultaneous exposure to EPO significantly reduced these effects. Rotenone enhanced mTOR expression and suppressed Beclin-1 expression, indicating suppression of the autophagy system. However, combined treatment with EPO restored Beclin-1 expression and decreased mTOR expression. EPO protects against rotenone-induced neurotoxicity in SH-SY5Y cells by enhancing autophagy-related signaling pathways. The experimental evidence for the EPO-induced neuroprotection against rotenone-induced dopaminergic neurotoxicity may significantly impact the development of future PD treatment strategies. © 2015, Springer Science+Business Media New York.


Kim M.-G.,Korea University | Kim S.H.,Gangneung Asan Hospital | Noh H.,Soonchunhyang University | Ko Y.S.,Korea University | And 4 more authors.
PLoS ONE | Year: 2013

Previous studies have shown that induction of immune tolerance by mesenchymal stem cells (MSCs) is partially mediated via monocytes or dendritic cells (DCs). The purpose of this study was to determine the role of CD11c+ cells in MSC-induced effects on ischemia/reperfusion injury (IRI). IRI was induced in wildtype (WT) mice and CD11c+-depleted mice following pretreatment with or without MSCs. In the in-vitro experiments, the MSC-treated CD11c+ cells acquired regulatory phenotype with increased intracellular IL-10 production. Although splenocytes cocultured with MSCs showed reduced T cell proliferation and expansion of CD4+FoxP3+ regulatory T cells (Tregs), depletion of CD11c+ cells was associated with partial loss of MSCs effect on T cells. In in-vivo experiment, MSCs' renoprotective effect was also associated with induction of more immature CD11c+ cells and increased FoxP3 expression in I/R kidneys. However all these effects induced by the MSCs were partially abrogated when CD11c+ cells were depleted in the CD11c+-DTR transgenic mice. In addition, the observation that adoptive transfer of WT CD11c+ cells partially restored the beneficial effect of the MSCs, while transferring IL-10 deficient CD11c+ cells did not, strongly suggest the important contribution of IL-10 producing CD11c+ cells in attenuating kidney injury by MSCs. Our results suggest that the CD11c+ cell-Tregs play critical role in mediating renoprotective effect of MSCs. © 2013 Kim et al.


Yun Y.H.,Seoul National University | Lee M.K.,Research Institute and Hospital | Park S.M.,Seoul National University | Kim Y.A.,Research Institute and Hospital | And 9 more authors.
Annals of Oncology | Year: 2013

Background: We evaluated whether complementary and alternative medicine (CAM) use influenced outcomes [survival and health-related quality of life (HRQOL)] of cancer patients whose condition had just been judged terminal. Patients and methods: From July 2005 to October 2006, we conducted a prospective cohort study of 481 terminally ill cancer patients at 11 university hospitals and the National Cancer Center in Korea. We assessed how the use of CAM affected HRQOL and survival. Results: In a follow-up of 481 patients and 163.8 person-years, we identified 466 deceased cases. On multivariate analyses, CAM users did not have better survival compared with nonusers [adjusted hazard ratio (aHR), 0.91; 95% confidence interval (CI) 0.74-1.10]. Among mind-body interventions, prayer showed significantly worse survival (aHR, 1.56; 95% CI, 1.00-2.43). Clinically, CAM users reported significantly worse cognitive functioning (-11.6 versus -1.3; P < 0.05) and fatigue (9.9 versus -1.0; P < 0.05) than nonusers. Compared with nonusers in subgroup analysis, users of alternative medical treatments, prayer, vitamin supplements, mushrooms, or rice and cereal reported clinically significant worse changes in some HRQOL subscales. Conclusion: While CAM did not provide any definite survival benefit, CAM users reported clinically significant worse HRQOLs. © The Author 2012. Published by Oxford University Press on behalf of the European Society for Medical Oncology. All rights reserved.


PubMed | Soonchunhyang University, Chungnam National University and Gangneung Asan Hospital
Type: Journal Article | Journal: Iranian journal of radiology : a quarterly journal published by the Iranian Radiological Society | Year: 2016

Central vein stenosis or occlusion is a common complication that can lead to significant morbidity and dysfunction of access in the hemodialysis patient. More lesions can develop over time, and preserving access becomes a challenge as life expectancy of the hemodialysis patient increases.The goal was to compare long-term results and determine the outcomes of open-cell stent versus closed-cell stent for central vein stenosis or occlusion in hemodialysis patients.From 1997 to 2015, in 401 hemodialysis patients, stent placement for central vein stenosis or occlusion was performed if balloon angioplasty was unsatisfactory, due to elastic recoil or occurrence of restenosis within 3 months. When thrombus was present, primary stenting was performed. A total of 257 open-cell stents and 144 closed-cell stents were used. Angiographic findings including lesion site, central vein stenosis or occlusion, and presence of thrombosis and complication were evaluated. Primary patency rate and mean patency rate of the stent were compared between two stent groups by Kaplan-Meier survival analysis.For the open-cell stent group, 159 patients were diagnosed as central vein stenosis and 98 were occlusion. For the closed-cell stent group, 78 were stenosis and 66 were occlusion. There were two complications for central migration and two for procedure-related vein rupture. Open-cell stents and closed-cell stents had mean patency rates of 10.9 0.80 months and 8.5 10.87 months, respectively (P = 0.002).The open-cell stent is effective and its performance is higher than that obtained with the closed-cell stent for treating central vein stenosis or occlusion in hemodialysis patients.


Kweon H.,National Academy of Agricultural Science | Lee S.-W.,Gangneung Asan Hospital | Hahn B.-D.,Korea Institute of Materials Science | Lee Y.-C.,Bestian Hospital | Kim S.-G.,Gangneung - Wonju National University
Journal of Oral and Maxillofacial Surgery | Year: 2014

Purpose: The objective of this study was to compare bone formation after installation of uncoated (UC), hydroxyapatite-coated (HA), collagen plus HA-coated (CH), and silk plus HA-coated (SH) implants. Materials and Methods: Implants in the UC group had acid-etched surfaces. Surface coating was applied using the aerosol deposition method. Cellular responses on the coated surfaces were examined with scanning electron microscopy. Cellular responses to the surfaces were studied with the corresponding coated discs and MG63 cells. Subsequently, 3-(4, 5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and alkaline phosphatase (ALP) assays were performed. Peri-implant bone formation was evaluated with the rabbit tibia model. Twenty-four implants from each group were installed. The animals were sacrificed 6 weeks after implant installation. Peri-implant bone formation and implant-to-bone contact were measured in histologic sections. Significance of differences across groups was evaluated using analysis of variance. Results: Scanning electron microscopic images showed that the CH and SH groups exhibited cells that appeared more spread out than those in the other groups. The SH group exhibited the highest value in the MTT assay. The CH group exhibited the highest level of ALP activity. Comparisons of these modifications with the acid-etched surfaces showed that the CH and SH groups displayed significantly greater periimplant bone formation (P < .001). Conclusion: The SH group displayed significantly greater new bone formation and bone-to-implant contact than did the other groups. © 2014 American Association of Oral and Maxillofacial Surgeons


Kang W.S.,Gangneung Asan Hospital | Lim H.W.,Gangneung Asan Hospital | Suh J.K.,University of Ulsan | Chung J.W.,University of Ulsan
NeuroReport | Year: 2012

This study investigated the effect of a zinc-deficient diet on the hearing in CBA mice and aimed to verify whether this hearing change is reversible by supplementation of zinc afterwards. We assessed hearing through an auditory brainstem response (ABR) with tone burst stimulation in 4, 8, 16, and 32 kHz and distortion product otoacoustic emissions in 5.6, 8, 11.3, and 16 kHz every week. The ABR threshold started to increase after 4 weeks on a zinc-deficient diet. The difference in the threshold between control and zinc-deficient animals became greater over time and plateaued at about 6 weeks. The ABR threshold differences between control and zinc-deficient mice were greater at higher frequencies. Four weeks of normal diet, following 8 weeks of a zinc-deficient diet, restored the ABR threshold to normal at all measured frequencies. Zinc-deficient mice did not show any distortion product otoacoustic emission threshold change at all frequencies. This finding suggests that a zinc-deficient diet increased the ABR threshold in CBA mice and a zinc-adequate diet restored the ABR threshold to normal. © 2012 Wolters Kluwer Health | Lippincott Williams & Wilkins.


Kang W.S.,Gangneung Asan Hospital | Hyun S.M.,University of Ulsan | Lim H.K.,University of Ulsan | Shim B.S.,Gangneung Asan Hospital | And 2 more authors.
Laryngoscope | Year: 2012

Objectives/Hypothesis: To assess the normative size of the cochlear nerve (CN) and facial nerve (FN) in normal-hearing ears and to determine whether nerve size varies with age. Study Design: Cross-sectional. Methods: We included 169 ears with normal hearing between 2005 and 2010. The height, width, and cross-sectional area (CSA) of the CN and FN in the middle of the internal auditory canal were measured on oblique sagittal images of 3.0-T magnetic resonance imaging. Results were compared by age. Young subjects were divided into three age groups, 0-5, 6-10, and 11-15 years. Subjects over age 40 years were divided into groups at 10-year intervals. Results: Mean age was 32.6 years (range, 0.75-79 years). We found that the CN had significantly greater vertical (1.10 ± 0.21 mm vs. 0.95 ± 0.21 mm) and horizontal (1.11 ± 0.20 mm vs. 1.03 ± 0.22 mm) diameters than the FN. The CSA of the CN was larger than that of the FN (0.98 ± 0.33 mm 2 vs. 0.79 ± 0.31 mm 2). Except for the CN vertical diameter, there were no significant differences between right and left ears. Sex did not affect the nerve size. Although the CN was not affected by age, the FN vertical diameter and CSA of children <5 years were significantly smaller than those of children aged >5 years. The size of the two nerves did not differ among groups over age 40 years. Conclusions: The CN is not affected by age in normal-hearing ears. The FN vertical diameter and CSA of children <5 years are smaller than those of older children. Copyright © 2012 The American Laryngological, Rhinological, and Otological Society, Inc.


Yi S.W.,Gangneung Asan Hospital | Ahn J.H.,Gangneung Asan Hospital
Fertility and Sterility | Year: 2010

A 37-year-old woman with secondary postpartum hemorrhage due to a pseudoaneurysm rupture of the uterus was given selective uterine arterial embolization, and she was discharged in stable condition on the fourth postembolization day. Secondary postpartum hemorrhage due to rupture of a pseudoaneurysm is a rare condition, and selective uterine arterial embolization is the treatment of choice in hemodynamically stable patients. © 2010 American Society for Reproductive Medicine.


Won J.H.,Ajou University | Bista A.B.,Ajou University | Bae J.I.,Ajou University | Oh C.K.,Ajou University | And 3 more authors.
American Journal of Roentgenology | Year: 2012

OBJECTIVE. The objective of our study was to evaluate the efficacy and safety of a venotomy and manual propulsion technique that is performed to treat failed native arteriovenous fistulas (AVFs) with chronic organized thrombi. MATERIALS AND METHODS. For this study, we retrospectively reviewed a total of 69 venotomy and manual propulsion procedures performed from October 2005 to July 2009 in 56 patients for the treatment of native AVFs occluded by chronic thrombi. Inflow, anastomotic, and outflow veins were occluded using balloon catheters. Venotomy was made in the thrombi-bearing vein, and thrombi were propelled toward the venotomy site in a "milking" manner and were removed. After repair of the venotomy using simple interrupted sutures, the occlusion balloons were deflated. Angioplasty of the underlying stenosis was performed. RESULTS. Technical success was achieved in 95.7% of the procedures and clinical success was achieved in 91.3%. The follow-up duration was 1-50 months (mean, 16.7 months), with 3-, 6-, and 12-month primary patency rates of 92.5%, 80.8%, and 58.1%, respectively, and secondary patency rates of 98.1%, 96.2%, and 91.7%. The complication rate was 7.24%, with two major and three minor complications. CONCLUSION. The venotomy and manual propulsion technique is effective and safe for the removal of chronic and organized thrombi from occluded native AVFs. © American Roentgen Ray Society.

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