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Sen S.K.,Gandhi Institute of Engineering Technology | Dora T.K.,Gandhi Institute of Engineering Technology | Bandyopadhyay B.,Vidyasagar University | Mohapatra P.K.D.,Vidyasagar University | Raut S.,Gandhi Institute of Engineering Technology
Biocatalysis and Agricultural Biotechnology | Year: 2014

In the present investigation the production and characterization of a thermostable alpha-amylase enzyme from hot spring isolates Alcaligenes faecalis SSB17 has been studied. The individual and combined effect of temperature, pH and time has been investigated on the production of enzyme in four media (Luria Bertani, Nutrient Media, Semi-Synthetic Media l and Semi-Synthetic Media 2) using Box-Behnken design (BBD). Analysis of variance (ANOVA) shows high coefficient values of determination for each medium and satisfactory prediction second order regression models has been derived. Enzyme purification has been carried out by ammonium sulfate precipitation and size exclusion chromatography (SEC). Maximum purification has been obtained by SEC with 60 fold. SDS-PAGE of the purified enzyme has shown the presence of a single band associated with the amylase enzyme, having a molecular weight of 52 kDa. © 2014 Elsevier Ltd. Source

Sen S.K.,In Gene Research Laboratory Biostadt India Ltd | Das M.M.,Gandhi Institute of Engineering Technology | Bandyopadhyay P.,In Gene Research Laboratory Biostadt India Ltd | Dash R.R.,Gandhi Institute of Engineering Technology | Raut S.,Gandhi Institute of Engineering Technology
Ecological Engineering | Year: 2016

The disposal of fly ash as a by-product of incineration of coal is a more significant problem than ash produced from burning of municipal solid wastes, sugarcane bagasse, rice husks or tea dusts because of its volume. The present investigation aims at upgrading alumina in coal fly ash (CFA) through bioleaching, using Bacillus barbaricus (silicate bacterium) isolated from Taptapani hot spring, Odisha. Bioleaching has been done after washing CFA (WCFA) with water and the result obtained is compared with the results obtained by bioleaching of unwashed CFA (UCFA). The concentration of alumina in washed coal fly ash has increased to 34.72% from an initial concentration of 25.45% after 60 days. The residue also shows a reduction of silica content from an initial concentration of 62.14% to 40.71% after 60 days. All the experimental results are corroborated with chemical analysis as well as through characterization process. Thus present finding has opened an avenue for enrichment of CFA with respect to alumina which may be suitable for aluminum extraction. In addition, by-product may be used for production of silica sol. © 2015 Elsevier B.V. Source

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