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Gāndhī Nagar, India

Sri Manjari K.,Osmania University | Nallari P.,Osmania University | Balakrishna N.,National Institute of Nutrition | Vidyasagar A.,Gandhi General Hospital | And 3 more authors.
Biochemical Genetics | Year: 2013

This study investigated the role of -1607 (1G/2G) (rs1799750) polymorphism of the MMP-1 gene in chronic pancreatitis. We genotyped 100 patients with chronic pancreatitis and 100 control subjects using tetra-primer ARMS-PCR followed by agarose gel electrophoresis. Serum levels of MMP-1 were determined by Elisa. Statistical analysis was applied to test the significance of the results. The genotypic and allelic distribution varied significantly between the disease group and the control subjects [OD = 1.981 (1.236-3.181), p = 0.004]. MMP-1 levels were higher in subjects homozygous for the 2G allele than in subjects with the 1G allele. The present study revealed a significant association of the MMP-1 -1607 1G/2G (rs1799750) gene promoter polymorphism with chronic pancreatitis, and it can be considered a biological marker in the etiology of chronic pancreatitis. © 2013 Springer Science+Business Media New York. Source

Sri Manjari K.,Osmania University | Jyothy A.,Osmania University | Shravan Kumar P.,Gandhi General Hospital | Nallari P.,Osmania University | Venkateshwari A.,Osmania University
Indian Journal of Medical Research | Year: 2014

Background & objectives: Chronic pancreatitis is progressive and irreversible destruction of the pancreas. Matrix metalloproteinase-7 (MMP-7) is a secreted matrilysin, which contributes to angiogenesis and breakdown of basement membranes of pancreatic tissues. The present study was aimed to investigate the association of MMP-7 -181A/G (rs11568818) gene promoter polymorphism in patients with chronic pancreatitis.Methods: A total of 100 chronic pancreatitis patients and 150 unrelated healthy individuals were included in this case control study. The genotyping of the MMP-7 gene (- 181A/G) (rs11568818) was carried out based on PCR-RFLP. The serum levels of MMP-7 were determined by ELISA. Association between genotypes and chronic pancreatitis was examined by odds ratio (OR) with 95% confidence interval (CI).Results: The frequencies of the genotypes in promoter of MMP-7 were AA 49 per cent, AG 25 per cent and GG 26 per cent in chronic pancreatitis patients and AA 53 per cent, AG 38 per cent and GG 9 per cent in control subjects. Frequency of MMP-7 -181GG genotype and - 181G allele was significantly associated with chronic pancreatitis compared to healthy subjects [OR = 1.58 (95% CI: 1.06 -2.36), P =0.019]. There was no significant difference in the serum MMP-7 levels in the patients compared to control subjects.Interpretation &conclusions: The present study revealed a significant association of MMP-7 -181A/G (rs11568818) GG genotype with chronic pancreatitis patients, indicating its possible association with the disease. © 2014, Indian J Med Res. All Rights Reserved. Source

Sri Manjari K.,Osmania University | Jyothy A.,Osmania University | Shravan Kumar P.,Gandhi General Hospital | Prabhakar B.,Osmania General Hospital | And 4 more authors.
Gene | Year: 2014

Objective: Chronic pancreatitis is a gradual, long-term inflammation of the pancreas that results in alteration of its normal structure and function. The study aims to investigate the role of - 308 (G/A) polymorphism of TNF-α gene in chronic pancreatitis. Material and methods: A total of 200 subjects were included in this case-control study. A total of 100 in patients admitted in the Gastroenterology Unit of Gandhi Hospital and Osmania General Hospital, Hyderabad were included in the present study. An equal number of healthy control subjects were randomly selected for the study. The genotyping of TNF-α gene was carried out by tetra-primer ARMS PCR followed by gel electrophoresis. The TNF-α levels were assayed by enzyme-linked immunosorbent assay. Results: A significant variation with respect to the genotypic and allelic distribution in the disease group when compared to control subjects [OR. = 2.001 (1.33-3.005), p. < 0.0001**] was observed. Subjects homozygous for the A allele had higher TNF-α levels compared to G allele. Conclusion: The present study revealed a significant association of the TNF-α gene promoter polymorphism with chronic pancreatitis. Thus, TNF-α genotype can be considered as one of the biological markers in the etiology of chronic pancreatitis. © 2014 Elsevier B.V. Source

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