Li X.,University of Sichuan |
Cai H.,Gan Su Provincial Hospital |
He J.,Gan Su Provincial Hospital |
Ramachandran D.,Haus Gilead i |
And 6 more authors.
Clinical and Experimental Hypertension | Year: 2015
Tibetan monks are a special group in life style and diet customs. We have little information of hypertension about them. Therefore, the objective of this study was to investigate the information on the prevalence, awareness, treatment and control of hypertension in these populations. A cross-sectional study of hypertension was carried out in 984 monks and 1042 Tibetan residents controls in the same area. All the subjects were selected for interview, and physical examination involved blood pressure (BP) measurement. The overall prevalence of hypertension in monks was significantly lower than those in local residents (19.3% versus 34.1%; p < 0.01). The rates of awareness, treatment and control in monks were also lower than those in local residents (9.5% versus 16.9%, 4.2% versus 13.2% and 1.6% versus 4.5%; p < 0.01 for each). These findings indicated that a low prevalence of hypertension among monks aged 18 years and over in Gannan Tibetan autonomous district of Gansu province in China. We concluded that the relatively healthy diet and ways of life in monks were the major contributing factors to the lower prevalence of hypertension. However, the awareness, treatment and control rates of hypertension were also low. The possible reasons were most likely due to the difficult access to quality medical care and poor health education. © 2015 Taylor & Francis Group, LLC.
Wu S.,Gan Su Provincial Hospital |
Gao X.,Gan Su Provincial Hospital |
Yang S.,Gan Su Provincial Hospital |
Meng M.,Gan Su Provincial Hospital |
And 2 more authors.
Fundamental and Clinical Pharmacology | Year: 2015
Our and other studies have reported that homocysteine thiolactone (HTL) could induce endothelial dysfunction. However, the precise mechanism was largely unknown. In this study, we tested the most possible factor-endoplasmic reticulum (ER) stress, which was demonstrated to be involved in endothelial dysfunction in cardiovascular disease. Acetylcholine (Ach)-induced endothelium-dependent relaxation (EDR) and biochemical parameters were measured in rat isolated aorta. The level of reactive oxygen species (ROS) and NO was designed by specific fluorescent probe DCFH-DA and DAF-FM DA separately. The nuclear translocation of the NF-κB was studied by immune-fluorescence. The mRNA expression and protein expression of GRP78-a key indicator for the induction of ER stress-were assessed by real-time PCR and Western blot. Two ER stress inhibitors-4-PBA (5 mm) and Tudca (500 μg/mL)-significantly prevented HTL-impaired EDR and increased NO release, endothelial nitric oxide synthase (eNOS) and SOD activity, decreased ROS production, NADPH activity, NOX-4 mRNA and MDA level. We also found that 4-PBA and Tudca blocked HTL-induced NF-κB activation thus inhibiting the downstream target gene production including TNF-α and ICAM-1. Simultaneously, HTL increased the mRNA and protein level of GRP78. HTL could induce ER stress leading to a downstream enhancement of oxidative stress and inflammation, which finally caused vascular endothelial dysfunction. © 2015 Société Française de Pharmacologie et de Thérapeutique.