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Bieruń, Poland

Stolarz M.,University of Silesia | Binkowski M.,University of Silesia | Ficek K.,Galen Orthopaedics | Ficek K.,University Of Szczecin | And 2 more authors.
Journal of Medical Imaging and Health Informatics | Year: 2016

The anterior cruciate ligament is one of the most important ligaments in the knee joint. The number of its injuries and operations has been rising in recent years. In response to this, research has been done in order to improve operating techniques and to work out new methods. Presently there is no commonly acknowledged methodology for assessing bone tissue quality at the site of the transplant integration. In previous studies authors used different criteria to analyze the bone structure directly adjacent to the transplanted ligament. The aim of the present study was to formulate a methodology for X-ray microtomography measurements and assessment of regeneration and growth of bone tissue within the area of integration of the anterior cruciate ligament graft in sheep, as well as to identify the changes taking place in the fibrocartilage callus. The results of the study revealed that spatial reslicing and measuring the segmented region constituting 120% of the diameter of the bone tunnel successfully show histomorphometric changes in the structure of bone tissue. Visualization of the changes in the Drishti program displayed and confirmed the applied measurements. The presented methodology for X-ray microtomographic assessment of growth and regeneration of bone tissue within the bone tunnel in the context of integration of transplanted ligaments may be useful for further studies on the reconstruction of the anterior cruciate ligament. © 2016 American Scientific Publishers All rights reserved. Source


Ficek K.,Galen Orthopaedics | Kaminski T.,Academy of Physical Education in Katowice | Wach E.,Galen Orthopaedics | Cholewinski J.,Galen Orthopaedics | Cieszczyk P.,University Of Szczecin
Journal of Human Kinetics | Year: 2011

Any new method of treatment is associated with high expectations for its success, particularly if the therapy is based not only on the premise of achieving a symptomatic effect, but also improving functional quality and repairing structurally damaged tissues. Platelet Rich Plasma (PRP) application was shown to be a successful catalyst in the healing process for a wide variety of conditions in animal and human models. However, its use has been controversial due to many types of the PRP definition, optimal concentration, and modalities of implementation. In the qualification of patients for PRP treatment, not only should medical indications be considered, but also the role of participation in therapy with a physiotherapist supervising physical parameters and techniques used during recovery time. Further study is required in order to define optimal handling procedures of PRP injection. Long-term follow up will reveal if the promise of this substance can be realized and implemented to maximize its potential as a therapeutic remedy. © Editorial Committee of Journal of Human Kinetics. Source


Ficek K.,Galen Orthopaedics | Ficek K.,University Of Szczecin | Ficek K.,Academy of Physical Education in Katowice | Filipek J.,Galen Orthopaedics | And 6 more authors.
Journal of Materials Science: Materials in Medicine | Year: 2016

The aims in treating patients diagnosed with critical-sized bone defects resulting from bone cysts are to replace the lost bone mass after its removal and to restore function. The standard treatment is autologous or allogeneic bone transplantation, notwithstanding the known consequences and risks due to possible bone infection, donor site morbidity, bleeding and nerve injury and possible undesirable immune reactions. Additionally, allogeneic grafts are inhomogeneous, with a mosaic of components with difficult-to-predict regenerative potential, because they consist of cancellous bone obtained from different bones from various cadavers. In the present study, a 22-year-old patient with a history of right humerus fracture due to bone cysts was diagnosed with recurrent cystic lesions based on X-ray results. The patient qualified for an experimental program, in which he was treated with the application of a bioresorbable polylactide hybrid sponge filled with autologous platelet-rich plasma. Computed tomography and magnetic resonance imaging performed 3, 6, and 36 months after surgery showed progressive ossification and bone formation inside the defect cavity in the humerus. Three years after treatment with the bone substitute, the patient is pain free, and the cystic lesions have not reoccurred. © 2015, Springer Science+Business Media New York. Source


Ficek K.,Galen Orthopaedics | Stepien-Slodkowska M.,University Of Szczecin | Kaczmarczyk M.,Pomeranian Medical University | Maciejewska-Karlowska A.,University Of Szczecin | And 6 more authors.
Balkan Journal of Medical Genetics | Year: 2014

One of the most severe injuries sustained by athletes is rupture of the anterior cruciate ligament (ACL). Recent investigations suggest that a predisposition for ACL rupture may be the result of specific genetic sequence variants. In light of this, we decided to investigate whether the COL12A1 A9285G polymorphism was associated with ACL ruptures in Polish football players. We compared genotypic and allelic frequencies of the COL12A1 A9285G polymorphism in two groups of athletes: 91 male football players (23 ± 3 years) with surgically diagnosed primary ACL ruptures who qualified for ligament reconstruction (cases) and 143 apparently healthy, male football players of the same ethnicity, a similar age category, and a comparable level of exposure to ACL injury, who were without any self-reported history of ligament or tendon injury (controls). DNA samples extracted from the oral epithelial cells were genotyped by using a real-time polymerase chain reaction (Ri-Ti-PCR) method. The genotype distribution in the cases were not different from those in controls (p = 0.70). The frequency of the G allele was lower in the cases (18.1%) but not statistically significant (p = 0.40) when compared with controls (21.3%). Our results are in contradiction to the hypothesis that the COL12A1 A9285G polymorphism is associated with a predisposition for ACL injury. However, these conclusions should be supported with more experimental studies on COL12A1 polymorphisms. Source


Ficek K.,Galen Orthopaedics | Ficek K.,University Of Szczecin | Cieszczyk P.,Academy of Physical Education in Poznan | Kaczmarczyk M.,University Of Szczecin | And 9 more authors.
Journal of Science and Medicine in Sport | Year: 2013

Objectives: To examine the association of the COL1A1 -1997G/T and +1245G/T polymorphisms, individually and as haplotypes, with anterior cruciate ligament ruptures in professional soccer players. Design: Subjects were 91 male professional soccer players with surgically diagnosed primary anterior cruciate ligament ruptures. The control group consisted of 143 apparently healthy male professional soccer players, who were without any self-reported history of ligament or tendon injury. Both subjects and healthy controls are from the same soccer teams, of the same ethnicity (Polish, East-Europeans for ≥3 generations), a similar age category, and had a comparable level of exposure to anterior cruciate ligament injury. Methods: Genomic DNA was extracted from the oral epithelial cells using GenElute Mammalian Genomic DNA Miniprep Kit (Sigma, Germany). All samples were genotyped using a Rotor-Gene real-time polymerase chain reaction. Results: Genotype distributions for both polymorphisms met the Hardy-Weinberg expectations in both subjects and controls (p>. 0.05). Higher frequency of the COL1A1 G-T (-1997G/T and +1245G/T polymorphisms) haplotype was significantly associated with reduced risk for anterior cruciate ligament rupture (Hap.score -1.98, p= 0.048). The TT genotype was under-represented in the anterior cruciate ligament rupture group. However, this result was not statistically significant (p= 0.084 Fisher's exact test, recessive mode: TT vs GT+GG). Conclusions: Higher frequency of the COL1A1 G-T haplotype is associated with reduced risk of anterior cruciate ligament injury in a group of professional soccer players. Consequently, carrying two copies the COL1A1 G-T haplotype may be protective against anterior cruciate ligament injury. © 2012 Sports Medicine Australia. Source

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