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Markic J.,University of Split | Jeroncic A.,University of Split | Polancec D.,Galapagos Research Center Ltd. | Bosnjak N.,University of Split | And 3 more authors.
European Journal of Pediatrics | Year: 2013

Early recognition of serious bacterial infection (SBI) in children is essential for better treatment outcome. Flow cytometry analysis of neutrophil surface molecules has been more frequently utilized as a tool for diagnosis of infection. The infants (n = 105) under 6 months of age presenting to the pediatric emergency department with fever without apparent source who were hospitalized with suspicion of having SBI were enrolled in this prospective study. Sixty-nine infants were included into the training pool and were classified into bacterial or viral infection group. Validation pool consisted of 36 infants. The values of white blood cells counts, absolute neutrophil count (ANC), C-reactive protein (CRP), procalcitonin (PCT), neutrophil CD11b, CD15s and CD64 expression, and the percentage (%CD15s+) and absolute count (AC-CD15s+) of CD15s+ neutrophils were determined. In infants with SBI, %CD15s+ was 10.5 times more likely to be higher than the cut-off value. ANC, CRP, PCT, CD64, and AC-CD15s+ were also found as useful biomarkers for differentiation between bacterial and viral infection. The best fit multivariate logistic regression model included CRP, PCT, and %CD15s+ as strong predictors of SBI. The model's sensitivity (87 %) and specificity (83 %) indicated high model's accuracy. After validation on independent dataset, model's accuracy maintained high: 86 % sensitivity and 93 % specificity, confirming its reliability and supporting CRP, PCT, and %CD15s+ as real predictors. The findings of this study support assumption made in the literature on significance of CD15s in inflammation processes. Also, this study demonstrated for the first time that CD15s is potentially valuable biomarker of SBI in infants. © 2013 Springer-Verlag Berlin Heidelberg.

Gregorek A.C.,University of Zagreb | Gornik K.C.,University of Zagreb | Polancec D.S.,Galapagos Research Center Ltd. | Dabelic S.,University of Zagreb
Biochemical Genetics | Year: 2013

Abdominal aortic aneurysm (AAA) is a complex genetic disorder caused by the interplay of genetic and environmental risk factors. The number of (GT) n repeats in the heme oxygenase-1 (HO-1) gene promoter modulates transcription of this enzyme, which might have anti-inflammatory, antioxidant, antiapoptotic, and antiproliferative effect. The distribution of alleles and genotypes in Croatian individuals genotyped for the (GT) n HO-1 polymorphism was similar to that in other European populations. Frequency of the short (S) alleles (GT < 25) was higher in AAA patients (41.9%) than in non-AAA individuals (28.2%, p = 0.0026) because there were more SL heterozygotes among the AAA patients. The SL genotype appeared to increase the risk for AAA, but the increase was not statistically significant after adjustment for age, sex, smoking, hypertension, and hyperlipidemia (OR = 1.53, 95% CI 0.90-3.09, p = 0.062). These findings contradict those of the only other study performed so far on the association of (GT) n HO-1 polymorphism and AAA. © 2013 Springer Science+Business Media New York.

Kosol S.,University of Graz | Schrank E.,University of Graz | Krajacic M.B.,Galapagos Research Center Ltd. | Wagner G.E.,University of Graz | And 5 more authors.
Journal of Medicinal Chemistry | Year: 2012

Interactions of macrolide antibiotics with biological membranes contribute to their bioavailability but are also involved in the formation of phospholipidosis, which is caused by the inhibition of phospholipase A 1 activity. We determined the interaction strength and localization of macrolide antibiotics with membrane-mimetics. Macrolides bind to membrane-mimetics with the positively charged amino groups being close to the micelle surface and thereby protect the lipids from being degraded by phospholipase A 1 rather than inhibiting the enzyme. © 2012 American Chemical Society.

Ljubas D.,University of Zagreb | Curkovic L.,University of Zagreb | Marinovic V.,Forensic Science Center Ivan Vucetic | Bacic I.,Forensic Science Center Ivan Vucetic | Tavcar B.,Galapagos Research Center Ltd.
Reaction Kinetics, Mechanisms and Catalysis | Year: 2015

Two nanostructured sol–gel TiO2 films were prepared on a glass substrate by means of the dip-coating technique with titanium (IV) isopropoxide as a precursor with and without the addition of polyethylene glycol (PEG) as a structure-directing agent. The synthesized films were characterized by using thermal gravimetry, differential scanning calorimetry, micro-Raman spectroscopy, and atomic force microscopy (AFM). Results of the AFM analysis revealed that both films are nanostructured and that the TiO2 film prepared with the addition of PEG has higher values of roughness. The photocatalytic activity of the films was evaluated by the photocatalytic degradation of the methyl orange monoazo dye and the congo red diazo dye with predominant radiation wavelengths of 365 nm (UV-A) and 254 nm (UV-C). The effects of temperature (17.5, 25 and 35 °C) on the film stability and on the degradation process were also followed. The TiO2 film created with the addition of PEG showed heightened photoactivity at all reaction temperatures and higher degradation rates of both dyes were observed with the UV-C than with the UV-A radiation. In some cases, the total decolorization process was complete in 90 or 120 min, but the total mineralization of the dye solutions was not achieved after 120 min. The TiO2 films were stable at all three temperatures after more than 50 working hours. The degradation processes of dyes were monitored by means of the UV/VIS spectrophotometry and the liquid chromatography mass spectrometry together with the total organic carbon. © 2015 Akadémiai Kiadó, Budapest, Hungary

Litvic M.,BELUPO Pharmaceuticals Inc. | Regovic M.,BELUPO Pharmaceuticals Inc. | Smic K.,BELUPO Pharmaceuticals Inc. | Lovric M.,BELUPO Pharmaceuticals Inc. | And 2 more authors.
Bioorganic and Medicinal Chemistry Letters | Year: 2012

Mo(VI) and Mo(V) salts both react selectively with Hantzsch esters to produce substitute pyridines in good-to-excellent yield (75-99%). The remarkable reactivity and selectivity of MoOCl4 under reflux of acetonitrile and MoCl5 in dichloromethane at room temperature encouraged us to propose that molybdenum-containing enzymes (such as xanthine or aldehyde oxidase) also participate to some degree in the metabolism of 1,4-dihydropyridine drugs in the liver analogous to NADH in the respiratory chain. © 2012 Elsevier Ltd. All rights reserved.

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