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Czapla-Masztafiak J.,Polish Academy of Sciences | Okon K.,Jagiellonian University | Galka M.,Gabriel Narutowicz City Specialist Hospital | Huthwelker T.,Paul Scherrer Institute | Kwiatek W.M.,Polish Academy of Sciences
Applied Spectroscopy | Year: 2016

The use of synchrotron radiation may shed more light on the study of prostate cancer, one of the leading diseases among men. In the presented study the microbeam setup at the PSI Swiss Light Source combined with fluorescence detected X-ray absorption spectroscopy (XAS) was applied to determine two-dimensional (2D) imaging of distributions of various chemical sulfur forms in prostate cancer tissue sections, since sulfur is considered important and essential in cancer progression. The research focused on prostate tissues obtained during routine prostatectomies on patients suffering from prostate cancer. Our previous studies using 1/4-XAS point measurements on prostate cancer cell lines showed the differences in fractions of various forms of sulfur between cancerous and non-cancerous cells. Therefore, in this experiment the chosen areas of prostate cancer tissues were scanned to get the full picture of the chemical composition of tissue, which is highly heterogeneous. The incident X-ray beams of energies tuned to spectroscopic features of the near-edge region of sulfur K-edge absorption spectra were used to provide contrast between chemical species presented in the tissue. Next, the relative content of the three main sulfur forms, found in biological systems, was calculated and the results are presented in a form of 2D color maps. These maps are correlated with the microscopic histological image of the scanned area. The main findings show that sulfur occurs in prostate tissue mainly in reduced form. The oxidized form of sulfur is present mostly in prostatic stroma, while sulfur in intermediate oxidation state is present in trace amount. © Society for Applied Spectroscopy. © The Author(s) 2015. Source


Chalcarz W.,Eugeniusz Piasecki University | Merkiel S.,Eugeniusz Piasecki University | Marzecka A.,Gabriel Narutowicz City Specialist Hospital | Godyn-Swedziol R.,Gabriel Narutowicz City Specialist Hospital
New Medicine | Year: 2012

Aim. The aim of this study was to assess knowledge about nutritional prevention of diet-related diseases in pregnant women from Kraków. Material and methods. Questionnaires on the knowledge about nutritional prevention of diet-related diseases were filled in by 115 pregnant women who were admitted to Gabriel Narutowicz City Specialist Hospital in Kraków. Statistical analysis was carried out by means of the IBM SPSS Statistics 19. The studied population was divided according to the trimester of pregnancy. Results. Statistically significant differences were found for the answers to question whether decreasing food intake may prevent obesity. All women in the first trimester of pregnancy knew the correct answer to this question, whereas the percentages of correct answers given by the women in the second and the third trimester were 73.1% and 66.7%, respectively. Conclusions. The studied pregnant women, irrespective of the trimester of pregnancy, were characterised by a high level of knowledge about nutritional prevention of diet-related diseases. Most probably, such a high level of knowledge in this field is the result of spreading in the mass media the information about the importance of nutritional factors in diet-related diseases prevention. © Borgis. Source


Marzecka A.,Gabriel Narutowicz City Specialist Hospital | Chalcarz W.,Eugeniusz Piasecki University | Merkiel S.,Eugeniusz Piasecki University | Popierz-Rydlewska N.,Eugeniusz Piasecki University | Godyn-Swedziol R.,Gabriel Narutowicz City Specialist Hospital
New Medicine | Year: 2012

Aim. The aim of this study was to assess knowledge about rich sources of vitamins and minerals in pregnant women from Kraków. Material and methods. Questionnaires on the knowledge about rich sources of vitamins and minerals were filled in by 115 pregnant women who were admitted to Gabriel Narutowicz City Specialist Hospital in Kraków. Statistical analysis was carried out by means of the IBM SPSS Statistics 19. The studied population was divided according to the trimester of pregnancy. Results. Statistically significant differences were found for the answers to seven questions. The women in the second trimester of pregnancy gave the most correct answers to as many as six of these questions, as well as to the remaining questions, statistically insignificant. Conclusions. The level of nutritional knowledge in the studied pregnant women was varied and similar to the level observed in the populations studied previously. Most probably, the major factor which influences nutritional knowledge of pregnant women is not education, but the duration of pregnancy, the necessity to adapt to new conditions, and whether the pregnancy had been planned. © Borgis. Source

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