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Bad Langensalza, Germany

Muller C.,Karlsruhe Institute of Technology | Wagner K.,Innovent Jena | Frankenfeld K.,Fzmb GmbH | Franzreb M.,Karlsruhe Institute of Technology
Biotechnology Letters | Year: 2011

Classical purification of the glycoprotein equine chorionic gonadotropin (eCG) from serum includes pH fractionation with metaphosphoric acid, two ethanol precipitation steps as well as dialysis followed by fixed-bed chromatography. A simplified process requiring only 1/3 of the solvent and improving the yield from 53 to 65% has been developed. The process comprises an ultra-/diafiltration step after the first ethanol precipitation, directly followed by an adsorption/desorption procedure based on magnetic microadsorbents with N,N-diethyl-ammonium functionalization. The process reaches an overall purification factor of eCG of more than 1800 and an average product activity of 1300 IUELISA/mg. After adapting the parameters of the fractionation and the type of magnetic microadsorbents, the new concept is likely to be transferable to other serum proteins. © 2011 Springer Science+Business Media B.V. Source


Schlufter K.,Friedrich - Schiller University of Jena | Schlufter K.,Fzmb GmbH | Heinze T.,Friedrich - Schiller University of Jena
Macromolecular Symposia | Year: 2010

The carboxymethylation of bacterial cellulose (BC) was studied under typical heterogeneous reaction conditions. It was found that the BC possesses a significantly lower reactivity compared to wood cellulose converted under comparable conditions. Moreover, water-solubility of carboxymethyl cellulose (CMC) obtained from BC appears at rather high degree of substitution of about 1.5 although a nearly statistical functionalization pattern was analyzed by HPLC. Obviously, the nano-structure of BC is important for the reactivity and the properties of the synthesized CMC like water-solubility. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source


Lee K.-Y.,Imperial College London | Tammelin T.,VTT Technical Research Center of Finland | Schulfter K.,Fzmb GmbH | Kiiskinen H.,VTT Technical Research Center of Finland | And 2 more authors.
ACS Applied Materials and Interfaces | Year: 2012

This work investigates the surface and bulk properties of nanofibrillated cellulose (NFC) and bacterial cellulose (BC), as well as their reinforcing ability in polymer nanocomposites. BC possesses higher critical surface tension of 57 mN m -1 compared to NFC (41 mN m -1). The thermal degradation temperature in both nitrogen and air atmosphere of BC was also found to be higher than that of NFC. These results are in good agreement with the higher crystallinity of BC as determined by XRD, measured to be 71% for BC as compared to NFC of 41%. Nanocellulose papers were prepared from BC and NFC. Both papers possessed similar tensile moduli and strengths of 12 GPa and 110 MPa, respectively. Nanocomposites were manufactured by impregnating the nanocellulose paper with an epoxy resin using vacuum assisted resin infusion. The cellulose reinforced epoxy nanocomposites had a stiffness and strength of approximately ∼8 GPa and ∼100 MPa at an equivalent fiber volume fraction of 60 vol.-%. In terms of the reinforcing ability of NFC and BC in a polymer matrix, no significant difference between NFC and BC was observed. © 2012 American Chemical Society. Source


Lee K.-Y.,Imperial College London | Ho K.K.C.,Imperial College London | Ho K.K.C.,Cytec Industries Inc. | Schlufter K.,Fzmb GmbH | Bismarck A.,Imperial College London
Composites Science and Technology | Year: 2012

A novel robust non-woven sisal fibre preform was manufactured using a papermaking process utilising nanosized bacterial cellulose (BC) as binder for the sisal fibres. It was found that BC provides significant mechanical strength to the sisal fibre preforms. This can be attributed to the high stiffness and strength of the BC network. Truly green non-woven fibre preform reinforced hierarchical composites were prepared by infusing the fibre preforms with acrylated epoxidised soybean oil (AESO) using vacuum assisted resin infusion, followed by thermal curing. Both the tensile and flexural properties of the hierarchical composites showed significant improvements over polyAESO and neat sisal fibre preform reinforced polyAESO. These results were corroborated by the thermo-mechanical behaviour of the (hierarchical) composites, which showed an increased storage modulus and enhanced fibre-matrix stress transfer. Micromechanical modelling was also performed on the (hierarchical) composites. By using BC as binder for short sisal fibres, added benefits such as the high Young's modulus of BC, enhanced fibre-fibre and fibre-matrix stress transfer can be utilised in the resulting hierarchical composites. © 2012 Elsevier Ltd. Source


Patent
Fzmb GmbH | Date: 2012-02-10

A process for detecting cells from a sample, comprising the following steps:

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