Zhang Y.,U.S. Center for Disease Control and Prevention |
Zhang Y.,Huazhong University of Science and Technology |
Tong Y.,U.S. Center for Disease Control and Prevention |
Zhang Y.,BGI Shenzhen |
And 12 more authors.
Molecular Neurobiology | Year: 2014
Genome-wide association studies (GWAS) of ischemic stroke (IS) have been performed on several cohorts of Caucasian or African population and Japanese, resulting in somewhat inconsistent conclusion. We aimed to identify susceptibility loci for IS by exome sequencing in a Chinese Han population. Exome sequencing was used to screen susceptibility loci among 100 cases and 100 matched controls. Significant SNPs from the first stage were verified in up to 3,554 participants from three hospital-based case-control studies. In the initial exome sequencing analysis, rs10489177 in c1orf156 gene located on chromosome 1q24 (p<1×10-8) and rs17118 in XYLB gene located on chromosome 3p21 (p<1×10-6) were found to be significantly associated with IS. In the following validation stage, significantly increased odds ratios were observed in individuals with rs10489177 GG (OR=2.02, 95 % CI=1.35-3.03) or rs17118 AA genotype (OR=1.50, 95 % CI=1.17-1.91). The rs10489177 GG genotype was associated with significantly increased risk for IS in individuals without hypertension (OR=2.78, 95 % CI=1.59-4.86) and in individuals without diabetes (OR=1.93, 95 % CI=1.27-2.94). In contrast, the rs17118 AA genotype may significantly increase the risk for IS, particularly for individuals with hypertension (OR=1.73, 95 % CI=1.08-2.78) and for individuals without diabetes (OR=1.52, 95 % CI=1.17-1.98) or non-smoker (OR=1.59, 95 % CI=1.16-2.19). Collectively, our study identified two novel loci (rs17118 and rs10489177) which were associated with an increased risk for IS in Chinese Han populations. Further studies are needed to confirm these associations in other populations and elucidate the biological mechanisms underlying the observed associations. © 2013 Springer Science+Business Media.
Sang Z.,Futian Hospital |
Fu Y.,Futian Hospital |
Chen D.,Futian Hospital |
Gu Y.-D.,Futian Hospital |
Chen X.-T.,Jiamusi University
Chinese Journal of Tissue Engineering Research | Year: 2015
BACKGROUND: Bacterial adhesion is closely related to the surface properties of cobalt-chromium alloys, and therefore, the surface modification technology has become the focus of research in this area. OBJECTIVE: To verify whether the cobalt-chromium alloy with zirconium nitride coating can improve the bacterial adhesion of metal denture materials. METHODS: Magnetron sputtering deposition method was used to plate zirconium nitride film on the surface of cobalt-chromium alloys (experimental group), and cobalt-chromium alloy specimens uncoated served as control group. Streptococcus mutans, Candida albicans, and Actinomyces viscosus were respectively inoculated on the two kinds of test specimen, and at end of culture, the colony counting was done. RESULTS AND CONCLUSION: In the bacterial adhesion test, the number of colonies of three kinds of bacteria in the experimental group was significantly lower than that in the control group (P < 0.05). The number of bacterial adhesion in the control group was significantly higher than that in the experimental group. These findings indicate that the cobalt-chromium alloy covered with zirconium nitride coating can significantly reduce the adhesion amount of Streptococcus mutans, Candida albicans and Actinomyces viscosus, and thus improve bacterial adhesion properties of cobalt-chromium alloys. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.