Ni G.-X.,Southern Medical University |
Shu B.,Third Military Medical University |
Huang G.,Fuqing Municipal Hospital |
Lu W.W.,University of Hong Kong |
Pan H.-B.,University of Hong Kong
Journal of Biomedical Materials Research - Part B Applied Biomaterials | Year: 2012
In this study, the effects of a series of strontium (Sr)-substituted HA ceramics (0, 1, 5, and 10 mol % Sr substitution) were tested on their physical and biological properties. The crystal structure, composition, and solubility were investigated by TEM, XRD, and solid titration solubility isotherms, respectively. In addition, rat MSCs were cultured with culture media containing ions released from the strontium-substituted HA ceramics as they dissolved. MTT test, alkaline phosphatase activity, and osteoblast transcription factor gene (cbfa1) expression were conducted at different time points. Our results suggested that HA with Sr substitution may change its physical properties, especially its solubility, and consequently enhance undifferentiated MSCs into osteoblast lineage. The results from this and the previous study suggested that 5-10 mol % Sr substitution into HA may be a suitable choice for its use in bone regenerative field. © 2011 Wiley Periodicals, Inc.
Yao Z.-P.,Fujian Medical University |
Huang G.-T.,Fuqing Municipal Hospital |
Liu W.-G.,Fujian Medical University |
Lu W.W.,University of Hong Kong
Journal of Materials Science: Materials in Medicine | Year: 2011
The purpose of this study was to test the effects of a series of strontium-substituted HA (Sr-HA) ceramics (0, 1, 5, and 10 mol% Sr substitution) on osteoblasts, thereby demonstrating whether strontium incorporation with HA would favor osteoblast metabolism. Rat primary osteoblasts were cultured with culture media containing ions released from the Sr-HA ceramics as they dissolved. MTT test, alkaline phosphatase activity, osteoblast transcription factor gene (cbfa1) expression and Alizarin Red staining were conducted at different time-points. There is no significant difference in cell proliferation between groups. However, compared with HA group, Sr-HA groups presented significant enhancement with regard to ALP activity, cbfa1 mRNA expression, and mineralization nodules. Among Sr-HA groups, 5 and 10% groups showed much better performances in ALP activity, cbfa1 mRNA expression, and mineralization nodules than 1% group, however, no significant difference was found between 5 and 10% groups. This study has demonstrated that Sr incorporation in HA ceramic enhanced osteoblastic cell differentiation and mineralization. However, further detailed studies are needed to understand the mechanistic effects of this Sr incorporation on osteoblastic cells and the optimal percentage of calcium should be substituted with strontium in HA. © Springer Science+Business Media, LLC 2011.
Xue F.,Fuqing Municipal Hospital |
Ye Y.-S.,Fuqing Municipal Hospital
Chinese Journal of Tissue Engineering Research | Year: 2015
BACKGROUND: Percutaneous vertebroplasty with bone cement injection for osteoporotic vertebral compression fractures can quickly relieve pain and increase vertebral strength, but vertebral re-fractures often occur because of osteoporosis. OBJECTIVE: To investigate the therapeutic effect of percutaneous vertebroplasty with bone cement injection combined with zoledronic acid in the treatment of osteoporotic vertebral compression fractures. METHODS: Six patients with osteoporotic vertebral compression fractures were enrolled, including two males and four females, aged 60-86 years. These patients with T12-L4 vertebral fractures underwent percutaneous vertebroplasty with polymethyl methacrylate acrylate bone cement treatment. At 4 weeks after bone cement injection, all patients were administrated with intravenous injection of zoledronic acid (100 mL: 5 mg) as anti-osteoporosis treatment, 30 drops per minute, once a year, for consecutive 2 years. The 2-year periodic imaging was performed to observe the clinical efficacy on vertebral compression fractures, bone density and adjacent vertebral fractures. RESULTS AND CONCLUSION: At 48 hours after bone cement injection, pain symptoms were relieved in the six cases. At the end of 2-year follow-up, fractures healed in all the six cases without delayed union or nonunion, and there was no bone cement leakage, nerve root compression, dural involvement, needle opening or wound infection and pulmonary embolism; the bone density was significantly higher than that before treatment (P < 0.05), and no adjacent vertebral fractures occurred. These findings indicate that the percutaneous vertebroplasty with bone cement injection combined with zoledronic acid can significantly relieve pain, improve vertebral stability and bone density, and reduce the incidence of adjacent vertebral fractures in patients with osteoporotic vertebral compression fractures. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.