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Torremolinos, Spain

Del Pilar Carrera M.,University of Jaen | Ramirez-Exposito M.J.,University of Jaen | Mayas M.D.,Fundacion Imabis | Garcia M.J.,Laboratorio Central Of Sanidad Animal | Martinez-Martos J.M.,University of Jaen
Tumor Biology | Year: 2010

Angiotensin II in particular and/or the local renin-angiotensin system in general could have an important role in epithelial tissue growth and modelling; therefore, it is possible that it may be involved in breast cancer. In this sense, previous works of our group showed a predominating role of angiotensin II in tumoral tissue obtained from women with breast cancer. However, although classically angiotensin II has been considered the main effector peptide of the renin-angiotensin system cascade, several of its catabolism products such as angiotensin III and angiotensin IV also possess biological functions. These peptides are formed through the activity of several proteolytic regulatory enzymes of the aminopeptidase type, also called angiotensinases. The aim of this work was to analyse several specific angiotensinase activities involved in the renin-angiotensin system cascade in mammary tissue from control rats and from rats with mammary tumours induced by N-methyl-nitrosourea (NMU), which may reflect the functional status of their target peptides under the specific conditions brought about by the tumoural process. The results show that soluble and membrane-bound specific aspartyl aminopeptidase activities and membrane-bound glutamyl aminopeptidase activity increased in mammary tissue from NMU-treated animals and soluble aminopeptidase N and aminopeptidase B activities significantly decreased in mammary tissue from NMU-treated rats. These changes support the existence of a local mammary renin-angiotensin system and that this system and its putative functions in breast tissue could be altered by the tumour process, in which we suggest a predominant role of angiotensin III. All described data about the renin-angiotensin system in mammary tissue support the idea that it must be involved in normal breast tissue functions, and its disruption could be involved in one or more steps of the carcinogenesis process. © 2010 International Society of Oncology and BioMarkers (ISOBM).

Gervasini G.,University of Extremadura | Agundez J.A.G.,University of Extremadura | Garcia-Menaya J.,Infanta Cristina University Hospital | Martinez C.,University of Extremadura | And 5 more authors.
Allergy: European Journal of Allergy and Clinical Immunology | Year: 2010

Background: Nonsynonymous polymorphisms in genes coding for histamine-metabolizing enzymes, diamine oxidase and histamine N-methyltransferase are related to the risk of developing allergic diseases. The role of polymorphisms in the histidine decarboxylase gene remains unexplored. The objective of this study is to identify novel polymorphisms in the human histidine decarboxylase gene and to analyse the clinical association of nonsynonymous polymorphisms with rhinitis. Methods: We performed a single-strand conformational polymorphism analysis of the histidine decarboxylase gene sequence. The presence of two nonsynonymous polymorphisms Thr31Met (rs17740607) and Glu644Asp (rs2073440) was analysed in 442 unrelated patients with allergic rhinitis, 233 of whom also had asthma, and in 486 healthy subjects. Results: We observed three novel polymorphisms designated as ss50402829, ss50402830 and ss50402831-(rs17740607) with allele frequencies=0.005, 0.208 and 0.073, respectively. Statistically significant differences were observed for the histidine decarboxylase Glu644Asp (rs2073440) polymorphism, with OR (95% CI) values for homozygous carriers of the Glu644 allele equal to 3.12 (1.75-5.56, P<0.00005) for all patients, 3.38 (1.54-7.44, P=0.002) for patients with rhinitis alone, and 2.92 (1.43-5.95), P=0.003 for patients with rhinitis+asthma, when compared with healthy controls. A significant Glu644 gene-dose effect was observed for overall patients (P=0.0001), for patients with rhinitis alone (P=0.005) and for patients with rhinitis+asthma (P=0.010). Conclusions: The HDC allele Glu644 in homozygosity increases the risk of developing rhinitis in the studied population. This adds to increasing evidence supporting a prominent role of genetic variations related to histamine homeostasis in the risk to develop allergic diseases. © 2010 John Wiley & Sons A/S.

Rodriguez-Bailon I.,Hospital Universitario Virgen Of La Victoria | Jimenez-Navarro M.F.,Hospital Universitario Virgen Of La Victoria | Perez-Gonzalez R.,Fundacion Imabis | Garcia-Orta R.,Hospital Universitario Virgen Of Las Nieves | And 2 more authors.
Revista Espanola de Cardiologia | Year: 2010

Segmental contractility can be assessed quantitatively by analyzing deformation, or strain, and the rate of deformation, or the strain rate. This type of analysis can be performed using either tissue Doppler imaging or, more recently, two-dimensional speckle-tracking echocardiography. The aim of this study was to determine typical parameter values in healthy subjects and their reproducibility. The study involved 105 healthy individuals, including 55 women (52.45%). Their mean age was 38.8±9.5 years (range, 20-59 years). All underwent speckle-tracking echocardiography with velocity vector imaging. Mean values for the strain and strain rate for each segment as well as for the time-to-peak normalized by the length of the cycle (TPN) were obtained. The resulting mean values were: circumferential strain, 22.2±4.81% with a TPN of 0.39±0.06; longitudinal strain, 19.84±4.59% with a TPN of 0.42±0.06; circumferential strain rate, 1.64±0.48 1/s with a TPN of 0.23±0.06; and longitudinal strain rate, 1.3±0.49 1/s with a TPN of 0.21±0.09. Intra- and inter-observer variability were moderate in magnitude.

Lopez-Moreno J.A.,Complutense University of Madrid | Lopez-Jimenez A.,Complutense University of Madrid | Gorriti M.A.,Complutense University of Madrid | Rodriguez De Fonseca F.,Fundacion Imabis
Current Drug Targets | Year: 2010

Although the first studies regarding the endogenous opioid system and addiction were published during the 1940s, addiction and cannabinoids were not addressed until the 1970s. Currently, the number of opioid addiction studies indexed in PubMed-Medline is 16 times greater than the number of cannabinoid addiction reports. More recently, functional interactions have been demonstrated between the endogenous cannabinoid and opioid systems. For example, the cannabinoid brain receptor type 1 (CB1) and mu opioid receptor type 1 (MOR1) co-localize in the same presynaptic nerve terminals and signal through a common receptor-mediated G-protein pathway. Here, we review a great variety of behavioral models of drug addiction and alcohol-related behaviors. We also include data providing clear evidence that activation of the cannabinoid and opioid endogenous systems via WIN 55,512-2 (0.4-10 mg/kg) and morphine (1.0-10 mg/kg), respectively, produces similar levels of relapse to alcohol in operant alcohol self-administration tasks. Finally, we discuss genetic studies that reveal significant associations between polymorphisms in MOR1 and CB1 receptors and drug addiction. For example, the SNP A118G, which changes the amino acid aspartate to asparagine in the MOR1 gene, is highly associated with altered opioid system function. The presence of a microsatellite polymorphism of an (AAT)n triplet near the CB1 gene is associated with drug addiction phenotypes. But, studies exploring haplotypes with regard to both systems, however, are lacking. © 2010 Bentham Science Publishers Ltd.

Soriguer F.,Hospital Regional Universitario Carlos Haya | Soriguer F.,CIBER ISCIII | Gutierrez-Repiso C.,Fundacion Imabis | Gonzalez-Romero S.,Hospital Regional Universitario Carlos Haya | And 9 more authors.
Clinical Nutrition | Year: 2011

Background & aims: The importance of milk intake to the supply of dietary iodine is not fully known. We therefore undertook a study in Spain of the iodine concentration in cow's milk and the impact of the frequency of milk consumption on urinary iodine concentrations in three study populations. Methods: We studied the iodine concentration in 362 samples of milk from 45 commercial brands and compared it with the milk iodine status in studies undertaken 17 years earlier. The epidemiologic studies were performed in three different places in the south of Spain: two in school-age children (N = 757 and N = 1205 children) and one in adults (N = 1051). A milk consumption questionnaire was given and urinary iodine concentrations measured. Results: The mean concentration of iodine in the milk rose from 1991 (117 ± 37 μg/L) to 2008 (259 ± 58 μg/L) (P < 0.001). The iodine concentration was greater in skimmed milk (273 ± 52 μg/L) than in semi-skimmed milk (254 ± 57 μg/L) or whole milk (251 ± 61 μg/L) (P < 0.0001). The winter samples had a greater concentration of iodine (270 ± 55 μg/L) than the summer samples (247 ± 58 μg/L) (P < 0.0001), independently of the type of milk. The urinary iodine concentrations in all three epidemiologic studies were significantly associated with the frequency of milk intake. Conclusions: The concentration of iodine in cow's milk has risen over recent years, and it is higher in skimmed milk. The results also show that cow's milk is a relevant source of dietary iodine. © 2010 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism.

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