Fundacao Centro Universitario Estadual da Zona Oeste
Fundacao Centro Universitario Estadual da Zona Oeste
Caxito M.L.C.,Federal University of Rio de Janeiro |
Victorio C.P.,Federal University of Rio de Janeiro |
Victorio C.P.,Fundacao Centro Universitario Estadual da Zona Oeste |
Da Costa H.B.,Federal University of Espirito Santo |
And 4 more authors.
Tropical Journal of Pharmaceutical Research | Year: 2017
Purpose: To investigate Euphorbia tirucalli extract for probable geographic variations in its antiproliferative activity. Methods: The aerial parts of E. tirucalli were collected in the Brazilian states of Mato Grosso, Rio de Janeiro, Pará, Minas Gerais and Santa Catarina. The 70% ethanol extract was obtained according to the procedure described in Brazilian Homeopathic Pharmacopeia. The antiproliferative activity of extracts, in concentrations of 62, 125, 250, and 500 µg mL-1, was tested against leukemia (HL-60), lymphoma (Daudi) and melanoma (B16F10) cell lines using methyl thiazol tetrazolium assay (MTT). Phytochemical analysis were carried out using High-performance liquid chromatography-diode array (HPLC-UV-DAD) and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI(-) FT-ICR MS) assays. Results: There was significant regional variability in the cytotoxicity of E. tirucalli extracts in a dose-dependent manner. The extracts had similar activity towards leukemia cell line HL-60, decreasing cell viability to about 60 – 70%. The extract showed the presence of ellagitannins, flavonoids, veracylglucan, and acid triterpenes as the major compounds. Conclusion: While the results support the ethnopharmacological use of E. tirucalli throughout Brazil, regional quantitative differences found in some classes of secondary metabolites may explain the variations observed in antitumor activity. © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved.
Oliveira S.S.,Instituto Nacional Of Controle Of Qualidade Em Saude |
Sorgine M.H.F.,Federal University of Rio de Janeiro |
Bianco K.,Instituto Nacional Of Controle Of Qualidade Em Saude |
Pinto L.H.,State University of Rio de Janeiro |
And 4 more authors.
Environmental Science and Pollution Research | Year: 2016
The identification of fecal pollution in aquatic ecosystems is one of the requirements to assess the possible risks to human health. In this report, physicochemical parameters, Escherichia coli enumeration and Methanobrevibacter smithii nifH gene quantification were conducted at 13 marine waters in the coastal beaches of Rio de Janeiro, Brazil. The pH, turbidity, dissolved oxygen, temperature, and conductivity, carried out by mobile equipment, revealed varied levels due to specific conditions of the beaches. The bioindicators’ enumerations were done by defined substrate method, conventional, and real-time PCR. Six marine beach sites (46 %) presenting E. coli levels in compliance with Brazilian water quality guidelines (<2500 MPN/100 mL) showed nifH gene between 5.7 × 109 to 9.5 × 1011 copies. L−1 revealing poor correlation between the two approaches. To our knowledge, this is the first inquiry in qPCR using nifH gene as a biomarker of human-specific sources of sewage pollution in marine waters in Brazil. In addition, our data suggests that alternative indicator nifH gene could be used, in combination with other markers, for source tracking studies to measure the quality of marine ecosystems thereby contributing to improved microbial risk assessment. © 2016 Springer-Verlag Berlin Heidelberg
Carvalho S.T.,Institute Biofisica Carlos Chagas Filho |
Oliveira I.A.,Institute Biofisica Carlos Chagas Filho |
Pita S.,Institute Biofisica Carlos Chagas Filho |
Goncalves A.S.,Institute Biofisica Carlos Chagas Filho |
And 11 more authors.
Glycobiology | Year: 2010
One of the most interesting aspects of Trypanosoma cruzi is its adaptation to obtain sialic acid from its host, fulfilling this need exclusively through the reaction catalyzed by enzymatically active trans-sialidase (aTS), thought to play an important role in the pathogenesis of Chagas' disease. Herein, we report that 2-difluoromethyl-4-nitrophenyl-3,5-dideoxy-D-glycero-α-D-galacto-2-nonulopyranosid acid (NeuNAcFNP) inactivates aTS time-and dose-dependently, and this inhibition was not relieved removing the inhibitor. Also, NeuNAcFNP causes a decrease in infection of mammalian cells. Characterization of labeled aTS by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry revealed that inactivation of the enzyme occurs through formation of a covalent bond between Arg245 and Asp247 and the inhibitor aglycone. Participation of Asp247 in the catalytic mechanism was proved by constructing a TSD247A mutant, which presents only residual activity. Molecular dynamic simulations indicate that the D247A mutation results in a more open catalytic cleft. In summary, NeuNAcFNP is the first reported mechanism-based inhibitor of aTS, representing a new template for drug design and opening new possibilities for chemotherapy of Chagas' disease, as well as for the elucidation of aTS function in T. cruzi pathogenesis and biology. © The Author 2010. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: firstname.lastname@example.org.
Jordao A.K.,Federal University of Fluminense |
Jordao A.K.,Fundacao Centro Universitario Estadual da Zona Oeste |
Vargas M.D.,Federal University of Fluminense |
Pinto A.C.,Federal University of Rio de Janeiro |
And 2 more authors.
RSC Advances | Year: 2015
Lawsone (1) is a special naphthoquinone that is useful for many applications in various scientific and technological fields. For over 100 years, it has been used as the starting material for the synthesis of a variety of biologically active compounds and materials with interesting properties. In organic synthesis, it has been used in many reactions. This review aims to address the various aspects of its use in organic synthesis. This journal is © The Royal Society of Chemistry.
Barros L.C.,Fundacao Centro Universitario Estadual da Zona Oeste |
Hirakawa A.R.,University of Sao Paulo
IEEE Latin America Transactions | Year: 2014
The representation and mapping of unknown environments for robot navigation is a focus of research that presents great challenges, especially regarding the amount of data involved and the difficulty of dynamically performing computational structures updating. This paper innovates by applying the Adaptive Digitized Straight Line Segment (ADSLS) technique to reduce the amount of data for representing trajectories through the Adaptive Finite Automata (AFA), improving positive results of state of the art works. © 2003-2012 IEEE.
Gomes Junior S.F.,Fundacao Centro Universitario Estadual da Zona Oeste |
Soares de Mello J.C.C.B.,Federal University of Fluminense |
Meza L.A.,Federal University of Fluminense
International Transactions in Operational Research | Year: 2013
Classic data envelopment analysis (DEA) models determine the efficiency of productive units, called decision making units (DMUs). DEA uses as its methodology the equiproportional reduction of inputs or increase of outputs and the finding of a single target for each DMU. This target does not incorporate the preference of the decision maker. Later works propose obtaining alternative targets based on nonradial projections on the efficiency frontier that are obtained through nonproportional variations of inputs or outputs. However, the efficiencies are not calculated for these alternative targets. This impedes a comparison among the DMUs. Thus, diverse nonradial efficiency indexes have been proposed based on mathematical averages or weighted averages that do not consider the vectorial characteristics of the efficiency. In this work, we present a nonradial efficiency index based on the initial concept of efficiency associated with each alternative (nonradial) target obtained through a multiobjective model of an inefficient DMU. © 2013 The Authors. International Transactions in Operational Research © 2013 International Federation of Operational Research Societies.
Correa S.C.A.,Brazilian National Nuclear Energy Commission |
De Aquino J.O.,Brazilian National Nuclear Energy Commission |
De Souza E.M.,Fundacao Centro Universitario Estadual da Zona Oeste |
Da Silva A.X.,Federal University of Rio de Janeiro
International Journal of Low Radiation | Year: 2011
In recent years, X-ray body scanners have been introduced at airports, penitentiaries and other places with considerable movement of people in order to combat drug trafficking, the entry of illegal materials and terrorism. However, although the application of this equipment in the national security area is indeed relevant, its use has caused a great deal of controversy, especially with regard to the doses absorbed and to the cancer induction risk associated with these exposures. The aim of this study is to use the Monte Carlo MCNPX code and the male adult voxel (MAX) and female adult voxel (FAX) phantoms, to evaluate the absorbed dose, effective dose and risk of cancer incidence attributable to exposures of individuals submitted to transmission X-ray body scanners in several projections. The effective dose values were calculated as recommended by the ICRP Publication 103 and the risk of cancer incidence were estimated through the BEIR VII Report. Copyright © 2011 Inderscience Enterprises Ltd.
PubMed | University Estadual Do Rio Of Janeiro Uerj, Instituto Nacional Of Controle Of Qualidade Em Saude, Federal University of Rio de Janeiro and Fundacao Centro Universitario Estadual da Zona Oeste
Type: Journal Article | Journal: Journal of applied microbiology | Year: 2016
To evaluate the diversity of Pseudomonads and antibiotic resistance profiles of Pseudomonas aeruginosa in a hospital wastewater treatment plant (HWTP) located in Rio de Janeiro city, Brazil. Due its intrinsic multidrug resistance and its ability to colonize several environments, we selected Ps.aeruginosa isolates as indicator of antimicrobial resistance frequency.Twenty-seven Ps. aeruginosa strains isolated from five stages of HWTP identified by rrs 16S rDNA sequencing were submitted against 12 antimicrobials through disc diffusion method. Among these isolates, 629% showed aztreonam resistance, followed by ticarcillin/clavulanic acid (333%) and cefepime (222%). Of these isolates, 222% were classified as multidrug-resistant (MDR3 classes). Five 16S rRNA gene libraries of Pseudomonas genus were constructed, one for each stages of the plant, yielding 93 sequences clustered in 41 Operational Taxonomic Units (OTUs). Each treatment step showed unique OTUs composition, suggesting changes in Pseudomonas spp. communities during the process. Several Pseudomonas species involved in biodegradation and bioremediation of xenobiotics were detected suggesting a positive impact in the wastewater treatment.Our strategy using metagenomics associated with the isolation of Ps.aeruginosa strains as bio-indicator allowed us to assess their antimicrobial susceptibility, the viability and diversity of Pseudomonas species in the hospital wastewater.The presence of MDR bacteria from treated effluents alerts for the need to improve these systems to avoid the spreading of resistance genes in aquatic ecosystems. This has special relevance in Brazil, where a significant portion of the population has no access to treated water.
PubMed | University of Porto, Fundacao Oswaldo Cruz, Fundacao Centro Universitario Estadual da Zona Oeste and Instituto Nacional Of Cancer Jose Alencar Gomes Da Silva
Type: Journal Article | Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | Year: 2016
Prostate cancer antigen 3 (PCA3) is a prostate-specific long noncoding RNA (lncRNA) involved in the control of prostate cancer (PCa) cell survival, through modulating androgen receptor (AR) signaling. To further comprehend the mechanisms by which PCA3 modulates LNCaP cell survival, we characterized the expression patterns of several cancer-related genes, including those involved in epithelial-mesenchymal transition (EMT) and AR cofactors in response to PCA3 silencing. We also aimed to develop a strategy to stably silence PCA3. Small interfering RNA (siRNA) or short hairpin RNA (shRNA) was used to knock down PCA3 in LNCaP cells. The expression of 84 cancer-related genes, as well as those coding for AR cofactors and EMT markers, was analyzed by quantitative real-time PCR (qRT-PCR). LNCaP-PCA3 silenced cells differentially expressed 16 of the 84 cancer genes tested, mainly those involved in gene expression control and cell signaling. PCA3 knockdown also induced the upregulation of several transcripts coding for AR cofactors and modulated the expression of EMT markers. LNCaP cells transduced with lentivirus vectors carrying an shRNA sequence targeting PCA3 stably downregulated PCA3 expression, causing a significant drop (60%) in the proportion of LNCaP cells expressing the transgene. In conclusion, our data provide evidence that PCA3 silencing modulates the expression of key cancer-related genes, including those coding for AR cofactors and EMT markers. Transducing LNCaP cells with an shRNA sequence targeting PCA3 led to loss of viability of the cells, supporting the proposal of PCA3 knockdown as a putative therapeutic approach to inhibit PCa growth.
PubMed | Instituto Nacional Of Controle Of Qualidade Em Saude, Federal University of Rio de Janeiro, State University of Rio de Janeiro and Fundacao Centro Universitario Estadual da Zona Oeste
Type: Journal Article | Journal: Environmental science and pollution research international | Year: 2016
The identification of fecal pollution in aquatic ecosystems is one of the requirements to assess the possible risks to human health. In this report, physicochemical parameters, Escherichia coli enumeration and Methanobrevibacter smithii nifH gene quantification were conducted at 13 marine waters in the coastal beaches of Rio de Janeiro, Brazil. The pH, turbidity, dissolved oxygen, temperature, and conductivity, carried out by mobile equipment, revealed varied levels due to specific conditions of the beaches. The bioindicators enumerations were done by defined substrate method, conventional, and real-time PCR. Six marine beach sites (46%) presenting E. coli levels in compliance with Brazilian water quality guidelines (<2500 MPN/100mL) showed nifH gene between 5.710