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Naseem M.,Functional Genomics and Systems Biology Group
Plant signaling & behavior | Year: 2013

Crosstalk between auxin and cytokinin in plant growth and development has already been established. However, their dynamics in plant immunity is still not well understood and requires systems biological approaches for analysis. Omics based public databases are exploited for the reconstruction, integration and analysis of Boolean models for hormonal interactions in plants. We established a meta-network by combining the plant immune regulatory network and the pathogen virulence specific network and used it as substrate for dynamic simulations on hormonal aspects of plant immunity. Our integrated analysis of this meta-network reveals antagonistic crosstalk between auxin and cytokinin in the Pst DC3000 and Arabidopsis interaction. Moreover, we discuss here the importance of Boolean models in unfolding inferences relevant to plant pathogen interactions. Source


Kaltdorf M.,Functional Genomics and Systems Biology Group | Naseem M.,Functional Genomics and Systems Biology Group
Science Signaling | Year: 2013

The small-molecule hormone salicylic acid (SA) is a plant immune signal for which the receptors have only recently been identifi ed. Two recent studies reported that the transcriptional coactivator nonexpresser of pathogenesis-related genes 1 (NPR1) and its paralogues NPR3 and NPR4 are bona fi de SA immune signal receptors in plants. Fu et al. demonstrated that because of their binding affinity for SA, NPR3 and NPR4 are SA receptors for immune responses in Arabidopsis thaliana. Both NPR3 and NPR4 function as adaptors in proteasomal degradation of NPR1 in an SA-dependent manner. By applying nonequilibrium methods, they showed very low binding affi nity of NPR1 for SA, suggesting that it may not qualify as an SA immune signal transduction receptor. However, using a method of equilibrium dialysis, Wu et al. found that SA binds to NPR1 and induces a conformational change in NPR1 or introduces steric hindrance that relieves repression of the transcriptional activation domain of NPR1 by an autoinhibitory N-terminal domain. This derepression leads to the expression of SA-dependent defense genes. Here, we discuss the importance of emerging SA perception models. Source


Naseem M.,Functional Genomics and Systems Biology Group | Kunz M.,Functional Genomics and Systems Biology Group | Ahmed N.,University for Information Science and Technology | Dandekar T.,Functional Genomics and Systems Biology Group
Plant Signaling and Behavior | Year: 2013

Crosstalk between auxin and cytokinin in plant growth and development has already been established. However, their dynamics in plant immunity is still not well understood and requires systems biological approaches for analysis. Omics based public databases are exploited for the reconstruction, integration and analysis of Boolean models for hormonal interactions in plants. We established a meta-network by combining the plant immune regulatory network and the pathogen virulence specific network and used it as substrate for dynamic simulations on hormonal aspects of plant immunity. Our integrated analysis of this meta-network reveals antagonistic crosstalk between auxin and cytokinin in the Pst DC3000 and Arabidopsis interaction. Moreover, we discuss here the importance of Boolean models in unfolding inferences relevant to plant pathogen interactions. © 2013 Landes Bioscience. Source


Naseem M.,Functional Genomics and Systems Biology Group | Srivastava M.,Functional Genomics and Systems Biology Group | Tehseen M.,King Abdullah University of Science and Technology | Ahmed N.,University of Balochistan
Current Protein and Peptide Science | Year: 2015

The plant hormone auxin regulates a whole repertoire of plant growth and development. Many plant-associated microorganisms, by virtue of their auxin production capability, mediate phytostimulation effects on plants. Recent studies, however, demonstrate diverse mechanisms whereby plant pathogens manipulate auxin biosynthesis, signaling and transport pathways to promote host susceptibility. Auxin responses have been coupled to their antagonistic and synergistic interactions with salicylic acid and jasmonate mediated defenses, respectively. Here, we discuss that a better understanding of auxin crosstalk to plant immune networks would enable us to engineer crop plants with higher protection and low unintended yield losses. © 2015 Bentham Science Publishers. Source


Naseem M.,Functional Genomics and Systems Biology Group | Kaltdorf M.,Functional Genomics and Systems Biology Group | Hussain A.,Abdul Wali Khan University Mardan | Dandekar T.,Functional Genomics and Systems Biology Group
Plant Signaling and Behavior | Year: 2013

Cytokinin has long been shown to be an essential modulator of growth and development in plants. However, its implications in plant immunity have only recently been realized. The interaction between jasmonate and salicylate pathways is regarded as a central backbone of plant immune defense. However, the effect of cytokinin on the jasmonate and salicylate mediated balance in plant immunity is still not known. Here, we analyze the impact of cytokinin on the jasmonate-salicylate antagonism in Arabidopsis immunity regarding infection with hemibiotrophic pathogen Pseudomonas syringae pv tomato DC3000 (Pst DC3000). Systems biology analysis of a refined hormone immune pathway model provides insights into the impact of cytokinin on the balance between jasmonate and salicylic acid pathways in Arabidopsis. Targeted experiments validate model simulations monitoring bacterial growth in wild type plants as well as in jasmonate pathway mutants. An integrated analysis shows that CK promotes the SA pathway of plant immunity and does not promote JA-mediated Arabidopsis susceptibility against infection with Pst DC3000. Finally, we discuss these results in the context of an emerging model of auxin-cytokinin antagonism in plant immunity. © 2013 Landes Bioscience. Source

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