FumoPrep Ltd.

Mórahalom, Hungary

FumoPrep Ltd.

Mórahalom, Hungary
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Szekeres A.,FumoPrep Ltd. | Szekeres A.,University of Szeged | Lorantfy L.,FumoPrep Ltd. | Lorantfy L.,Eötvös Loránd University | And 5 more authors.
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2013

Fumonisin B1 (FB1) is a highly toxic mycotoxin produced by fungal strains belonging to the Fusarium genus, which can be found mainly in maize products, and is gaining interest in food safety. To produce large amounts of pure FB1, a novel purifying method was developed by using centrifugal partition chromatography, which is a prominent member of the liquid-liquid chromatographic techniques. Rice cultured with Fusarium verticillioides was extracted with a mixture of methanol/water and found to contain 0.87 mg of FB1 per gram. The crude extracts were purified on a strong anion-exchange column and then separated by using a biphasic solvent system consisting of methyl-tert-butyl-ether-acetonitrile-0.1% formic acid in water. The collected fractions were analysed by flow injection-mass spectrometry and high-performance liquid chromatography coupled with Corona-charged aerosol detector and identified by congruent retention time on high-performance liquid chromatography and mass spectrometric data. This method produced approximately 120 mg of FB1 with a purity of more than 98% from 200 g of the rice culture. The whole purification process is able to produce a large amount of pure FB1 for analytical applications or for toxicological studies. © 2013 Copyright Taylor and Francis Group, LLC.


Szecsi A.,Hungarian Academy of Sciences | Szekeres A.,FumoPrep Ltd. Co. | Bartok T.,FumoPrep Ltd. Co. | Bartok T.,Cereal Research Non profit Ltd. Co. | And 3 more authors.
World Mycotoxin Journal | Year: 2010

The fumonisin B1-4 (FB1-4) toxin-producing capacities of 60 strains of Fusarium verticillioides isolated in the main maize-cultivating areas of Hungary were screened on rice grains in vitro. The amounts of FB1, FB2, FB3 and FB4 in the extracts of the culture material were determined by RP-HPLC/ESI-IT MS without any sample clean-up. All F. verticillioides strains produced all analogues (FB1-4) of the fumonisin B series. The strains did not differ significantly in their cultural characteristics, though the analytical results allowed the distinction of three idiosyncratic FB1-4- producing chemotypes with characteristic proportions of the fumonisin B analogues. The dominant chemotype produced them in the sequence FB1 > FB2 > FB3 > FB4, with large amounts of FB1 and FB2. A second chemotype produced a higher amount of FB3; than of FB2, while the third chemotype produced large amounts of FB2 and FB4. The proportion of FB1 in the extracts was inversely correlated with the total FB yield. No differences in FB1-4 producing capacity were observed between strains isolated from various locations or different sources. © 2010 Wageningen Academic Publishers.


Varga J.,University of Szeged | Kocsube S.,University of Szeged | Suri K.,University of Szeged | Szigeti G.,University of Szeged | And 4 more authors.
International Journal of Food Microbiology | Year: 2010

Aspergillus niger isolates are able to produce fumonisins in high quantities on agar media with a low water activity. Several agricultural products fit this criterion, including dried vine fruits, dates and figs. Data on the occurrence and role of this species in fumonisin contamination of agricultural products with high sugar content are needed to clarify the importance of A. niger in human health. The mycobiota and fumonisin contamination of various dried vine fruit samples collected from different countries were examined to clarify the role of black Aspergilli in fumonisin contamination of such products. All except two of the examined samples were contaminated with black Aspergilli. Species assignment of the isolates was carried out using sequence analysis of part of the calmodulin gene. The range of fumonisin isomers present in the raisin samples, and produced by A. niger isolates collected from dried vine fruits was also examined using reversed-phase high-performance liquid chromatography/electrospray ionization-ion trap mass spectrometry (RP-HPLC/ESI-ITMS). Among the 30 A. niger/A. awamori isolates identified, 20 were found to be able to produce fumonisins (average contamination: 5.16mg/kg; range: 0.017-19.6mg/kg). The average fumonisin content of the 7 dried vine fruit samples which were found to be contaminated by potential fumonisin producing black Aspergilli was 7.22mg/kg (range: 4.55-35.49mg/kg). The isolates produced several fumonisin isomers also present in the dried vine fruit samples, including fumonisins B1-4, 3-epi-FB3, 3-epi-FB4, iso-FB1, and two iso-FB2,3 forms. Fumonisin B1 was detected for the first time in A. niger cultures. Most of these isomers have previously only been identified in Fusarium species. Our data indicate that A. niger and A. awamori are responsible for fumonisin contamination of dried vine fruits worldwide. The observed levels of contamination are alarming and pose a new threat for food safety. © 2010 Elsevier B.V.


Bartok T.,Cereal Research Non Profit Ltd. Co. | Bartok T.,FumoPrep Ltd. Co. | Tolgyesi L.,Eötvös Loránd University | Tolgyesi L.,Kromat Ltd. Co. | And 6 more authors.
Rapid Communications in Mass Spectrometry | Year: 2010

Fumonisin mycotoxins which are hazardous to humans and animals were produced in a Fusarium verticillioides-infected solid rice culture. To decrease the possibility of the formation of artifacts, the fumonisins were analysed by reversed-phase high-performance liquid chromatography/electrospray ionization time-of-flight (RP-HPLC/ESI-TOFMS) and ion trap mass spectrometry (RP-HPLC/ESI-ITMS) immediately after the extraction of the culture material, without any further sample clean-up. The fumonisin isomers were separated by using a flat gradient on a special, high-coverage C18, narrow-bore HPLC column (YMC-Pack J'sphere ODS H80) suggested for the separation of structural isomers by the manufacturer. Exact mass measurements (TOFMS) of the protonated molecules and extraction of the ion chromatogram corresponding to the empirical formula (C34H59NO15) of FB1 toxins led to the identification of 29 peaks and shoulders, including those of FB1. The FB1 toxin and 28 of its isomers were also detected by ITMS after separation with RP-HPLC. The characteristic m/z values of the product ions, including the backbones obtained by ITMS2, undoubtedly indicated the structures of the FB1 isomers for 28 peaks and shoulders. In the MS2 spectra of the protonated molecules of the FB1 isomers, with some exceptions, 15 characteristic product ions including the hydrocarbon backbone at m/z 299 were observed. The abundance ratio of the cation at m/z 299 ranged up to 5.8%. The relative quantities of the isomers found in the sample extract were expressed as percentages of the FB1 content (0.001-0.579%). The total amount of the 28 FB1 isomers was 2.803% of the quantity of FB1 that is important from the aspect of food and feed safety. © 2009 John Wiley & Sons, Ltd.


Csernetics A.,University of Szeged | Nagy G.,University of Szeged | Iturriaga E.A.,University of Salamanca | Szekeres A.,FumoPrep Ltd. | And 3 more authors.
Fungal Genetics and Biology | Year: 2011

The zygomycete Mucor circinelloides accumulates β-carotene as the main carotenoid compound. In this study, the applicability of some early genes of the general isoprenoid pathway to improve the carotenoid production in this fungus was examined. The isopentenyl pyrophosphate isomerase gene (ipi) was cloned and used together with the genes encoding farnesyl pyrophosphate synthase (isoA) and geranylgeranyl pyrophosphate synthase (carG) in overexpression studies. Transformation experiments showed that the first bottleneck in the pathway, from the aspect of carotenoid production, is the step controlled by the carG gene, but overexpression of the ipi and isoA genes also contributes to the availability of the precursors. Transformations with these isoprenoid genes in combination with a bacterial β-carotene ketolase gene yielded Mucor strains producing canthaxanthin and echinenone. © 2011 Elsevier Inc.


Kredics L.,University of Szeged | Laday M.,Hungarian Academy of Sciences | Kormoczi P.,University of Szeged | Manczinger L.,University of Szeged | And 3 more authors.
Acta Biologica Szegediensis | Year: 2012

One hundred and sixteen Trichoderma isolates were collected from chopped roots of winter wheat of five agricultural fields in the Great Hungarian Plain. The isolates were identified by the sequence analysis of the internal transcribed spacer (ITS) region with TrichOKEY 2.0 and BLAST similarity searches. The Trichoderma species detected in the samples were T. atroviride, T. brevicompactum, T. gamsii, T. harzianum, T. koningiopsis/T. ovalisporum, T. longibrachiatum/H. orientalis, T. pleuroticola, T. rossicum, T. spirale, T. tomentosum/T. cerinum and T. virens. Beneficial taxa widely used as biocontrol agents against plant pathogenic fungi (e.g. T. harzianum, T. virens, T. atroviride) could be isolated from the samples examined during this study, indicating that the winter wheat rhizosphere may be a rich source of potential biocontrol isolates. Several species could be characterized with well-defined isoenzyme patterns during cellulose-acetate electrophoresis, suggesting that this method can be used for the analysis of biochemical diversity between and within particular species of the genus Trichoderma.


Nagy G.,University of Szeged | Imre G.,University of Szeged | Csernetics A.,University of Szeged | Petkovits T.,University of Szeged | And 4 more authors.
Acta Biologica Szegediensis | Year: 2011

In this study, a prenyl pyrophosphate synthase gene was cloned and characterized in the zygomycete fungus Gilbertella persicaria. The coding region of the isolated gene comprises a total of 1328 bp determining a 350 amino acids length putative protein and five introns (60, 65, 43, 59, and 48 bp in length) dispersed in the whole coding region. Based on the analysis of the nucleotide and the corresponding amino acid sequence, the gene encodes a farnezyl pyrophosphate synthase, and it was named as isoA. In a phylogeny inferred from amino acid sequences of fungal prenyl pyrophosphate synthases, the Gilbertella isoA proved to be the most closely related to that of Mucor circinelloides. Carotenoid composition of G. persicaria was also investigated: it produces β-carotene as the main carotenoid, but it also contains small amounts of β-cryptoxanthin, zeaxanthin and astaxanthin.


PubMed | FumoPrep Ltd.
Type: Journal Article | Journal: Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment | Year: 2012

Fumonisin B1 (FB1) is a highly toxic mycotoxin produced by fungal strains belonging to the Fusarium genus, which can be found mainly in maize products, and is gaining interest in food safety. To produce large amounts of pure FB1, a novel purifying method was developed by using centrifugal partition chromatography, which is a prominent member of the liquid-liquid chromatographic techniques. Rice cultured with Fusarium verticillioides was extracted with a mixture of methanol/water and found to contain 0.87 mg of FB1 per gram. The crude extracts were purified on a strong anion-exchange column and then separated by using a biphasic solvent system consisting of methyl-tert-butyl-ether-acetonitrile-0.1% formic acid in water. The collected fractions were analysed by flow injection-mass spectrometry and high-performance liquid chromatography coupled with Corona-charged aerosol detector and identified by congruent retention time on high-performance liquid chromatography and mass spectrometric data. This method produced approximately 120 mg of FB1 with a purity of more than 98% from 200 g of the rice culture. The whole purification process is able to produce a large amount of pure FB1 for analytical applications or for toxicological studies.

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