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Zhang X.,Chongqing Medical University | Deng W.,Fuling Center Hospital of Chongqing City | Ban Y.,Chongqing Medical University | Gao J.,Chongqing Medical University | Ding M.,Chongqing Medical University
Analyst | Year: 2013

A capillary electrically driven assay with electrochemiluminescent (ECL) detection for total bile acids in human serum was developed and fully validated. Quantification was performed by multiple reactions. First, the bile acids react with nicotinamide adenine dinucleotide (NAD+) under catalysis of 3α-hydroxysteroid dehydrogenase (3α-HSD), which is converted to 3-ketosteroid and concomitantly NAD+ turns into reduced nicotinamide adenine dinucleotide (NADH). And then Ru(bpy)3 2+ is oxidized to be Ru(bpy)3 3+, which serves as an electron mediator, and reacts immediately with NADH coexisting in a carrier solution and is converted to Ru(bpy)3 2+*. NADH transfers an electron to form NAD+ and the unstable excited-state species, Ru(bpy)3 2+*, which emits photons and gives out light when it decays to the ground state, Ru(bpy)3 2+. Consequently, the concentration of total bile acids could be determined by the electrochemiluminescent intensity. The assay was linear from 0.1 fmol L -1 to 1000 fmol L-1, with a detection limit of 0.02 fmol L-1. The intra-day and inter-day precision had a coefficient of variation of less than 5.0%. The developed ECL assay had an acceptable correlation with an enzymatic cycling method commonly adopted in clinics for the determination of total bile acids (r = 0.7216). Based on the above-mentioned principle, we established a simple, accurate and highly sensitive approach for the determination of total bile acids. Furthermore, this assay has been applied successfully to the detection of total bile acids in human serum, indicating its practicality for bioanalysis. This journal is © The Royal Society of Chemistry.

Qing M.,Fuling Center Hospital of Chongqing City | Qiu L.,Chongqing Medical University | Gao Z.,Second Affiliated Hospital of ChongQing Medical | Bhandari K.,Jiamusi University
Journal of Forensic and Legal Medicine | Year: 2014

The purpose of the study was to estimate the chronology of third molar mineralization in Han population of southwestern China and find its unique characteristics so that it would provide a reference in several legal cases like forensic age estimation. The study used Demirjian's staging method to study 2192 orthopantomograms of 984 male and 1208 female subjects aged between 8 and 25 years. The statistical data was analyzed by Student's t test and ANOVA. The conclusions of the study are: (1) The chronological mineralization age of third molars of Han population in Southwestern China is similar to the Turkish and the Japanese, was earlier than the Austrian and Han of South China, but later than the Spanish. (2) The mineralization timing of the third molars between two sides in maxilla or mandible has no significant differences in the same gender group. (3) There is no significant difference in mineralization of third molars between male and female, except for tooth 48 in Demirjian's stage E. (4) The mineralization of third molar in maxilla is earlier than mandible. © 2014 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.

Fu X.,Chongqing Medical University | Zhou Q.,Fuling Center Hospital of Chongqing City | Zhang X.,Chongqing Medical University
Medicine (United States) | Year: 2016

It remains unclear whether the efficacy of nonsurgical organ-preservation modalities (NOP) in the treatment of advanced-stage laryngeal cancer was noninferiority compared with that of total laryngectomy (TL). The objective of this study was to compare the curative effects between TL and NOP in the treatment of advanced-stage laryngeal cancer through a meta-Analysis. Clinical studies were retrieved from the electronic databases of PubMed, Embase, Wanfang, and Chinese National Knowledge infrastructure. A meta-Analysis was performed to investigate the differences in the curative efficacy of advanced-stage laryngeal cancer between TL and the nonsurgical method. Two reviewers screened all titles and abstracts, and independently assessed all articles. All identified studies were retrospective. Sixteen retrospective studies involving 8308 patients (4478 in the TL group and 3701 in the nonsurgical group) were included in this meta-Analysis. The analysis results displayed the advantage of TL for 2-year and 5-year overall survival (OS)(OR 2.79, 95% CI 1.85-4.23 and OR 1.52, 95% CI 1.09-2.14) as well as in 5-year disease-specific survival (DSS)(OR 1.79, 95% CI 1.61-1.98), but no significant difference in 2-year DSS was detected between the 2 groups (OR=2.09,95% CI0.69-6.40). Additionally, there were no significant differences between TL and NOP for 5-year local control (LC) either (OR=1.75, 95% CI 0.87-3.53). When we carried out subgroup analyses, the advantage of TL was especially obvious in T4 subgroups, but not in T3 subgroups. This is the first study to compare the curative effects on advanced-stage laryngeal cancer using meta-Analytic methodology. Although there was a trend in favor of TL for OS and DSS, there is no clear difference in oncologic outcome between TL and NOP. Therefore, other factors such as tumor T-stage and size, lymph node metastasis, and physical condition are also important indicators for treatment choice. © 2016 Wolters Kluwer Health, Inc. All rights reserved.

He Y.,Fuling Center Hospital of Chongqing City | He Y.,Central South University | Jia S.-B.,Central South University | Zhang W.,Fuling Center Hospital of Chongqing City | Shi J.-M.,Central South University
International Journal of Ophthalmology | Year: 2013

Uveitis is one of the most important causes of blindness worldwide. Its etiology and pathogenesis are complicated and have not been well understood. The treatment for uveitis is predominantly based on steroids and immunosuppressants. However, systemic side effects limit their clinical application. With the advancement of molecular biology, some intravitreal implants and biologic agents have been used for the treatment of uveitis. Additionally, novel techniques such as gene therapy and RNA interference are being studied for using as uveitis therapy. This paper reviews recent advances in uveitis treatment. Copyright International Journal of Ophthalmology Press.

Tao T.,Fuling Center Hospital of Chongqing City | Zhu X.-H.,Fuling Center Hospital of Chongqing City | Zhang X.-B.,Fuling Center Hospital of Chongqing City | Gu C.-Y.,Fuling Center Hospital of Chongqing City | And 2 more authors.
Journal of Shanghai Jiaotong University (Medical Science) | Year: 2014

Objective: To investigate the effects of a disintegrin and metalloprotease 33 (ADAM33) on the proliferation and apoptosis of human airway smooth muscle cells (HASMCs) and the effects of interleukin-4 (IL-4) on the expression of ADAM33.Methods: The HASMCs were stimulated by IL-4 in concentrations of 1, 10, 50, and 100 ng/mL. The unstimulated HASMCs were used as controls. The expressions of ADAM33 mRNA and ADAM33 protein were detected by the Real-Time PCR and Western blotting. ADAM33-siRNA was designed, synthesized, and transiently transfected to HASMCs. siRNA with the highest inhibition rate was screened by the Real-Time PCR and Western blotting and transiently transfected to HASMCs. The proliferation and apoptosis of cells were detected by the MTS and flow cytometry, respectively.Results: The expressions of ADAM33 mRNA and ADAM33 protein were concentration-dependent for different concentrations of IL-4. Compared to the control group, the relative expressions of mRNA and protein of groups with IL-4 of 50 ng/mL and 100 ng/mL increased by 5.94 and 7.6 times, and 3.72 and 4.57 times, respectively. The differences were statistically significant (P<0.05). But the relative expressions of mRNA and ADAM33 protein of groups with IL-4 of 1 ng/mL and 10 ng/mL were not significant increased. The differences were not statistically significant (P>0.05). For three siRNA, the inhibition rate of ADAM33-siRNA-575 was the highest. The inhibition rate was 81.08% at the mRNA level. The cell proliferation of the intervention group was significantly lower than that of the negative control group at 24, 48, and 72 h (P<0.05). The inhibition rates were 15.38%, 32.83%, and 22.78%, respectively. The apoptotic rate of the intervention group was (25.88±3.37)%, which was significantly higher than that of the negative control group [(8.95±0.51)%] (P<0.05).Conclusion: ADAM33 can promote the proliferation of airway smooth muscle cells and cytokine IL-4 can promote its expression. Both of them may play important roles in airway remodeling.

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