Fukuoka Industrial Technology Center

Fukuoka, Japan

Fukuoka Industrial Technology Center

Fukuoka, Japan
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Kusumoto K.-I.,Fukuoka Industrial Technology Center | Ishikawa T.,Fukuoka Industrial Technology Center
Journal of Controlled Release | Year: 2010

Didodecyldimethylammonium bromide (DDAB) is widely used for an efficient delivery system into mammalian cells. However, the biological activities of DDAB nanoparticles in mammalian cells are insufficiently understood. The purpose of this study was to establish a critical role of DDAB in cellular response. Here, we demonstrate that DDAB is a potent inducer of cell death in a wide range of tumor cell lines, wherein leukemia cells (HL-60 and U937) and neuroblastoma cells (Neuro2a) were more sensitive to DDAB than carcinoma cells such as HepG2 and Caco-2 cells. Moreover, in HL-60 cells, treatment with DDAB led to increased numbers of apoptotic cells with fragmented DNA (99.6%) and high levels of caspase-3 activation in comparison to that with actinomycin D. Cotreatment with a caspase-8 inhibitor (Z-IETD-FMK) or a polyethylene glycol (Mr 2000) (PEG 2000) effectively prevented the activation of caspase-3 induced by DDAB. These results suggest that DDAB can trigger caspase-3-mediated apoptosis through the extrinsic caspase-8 pathway and cytotoxic pore formation in cell membrane. Therefore, the present findings provide new insight into the biological activity of DDAB to induce caspase-mediated apoptosis. © 2010 Elsevier B.V.


Nishida H.,Kyushu Institute of Technology | Andou Y.,Kyushu Institute of Technology | Watanabe K.,Kyushu Institute of Technology | Arazoe Y.,Kyushu Institute of Technology | And 2 more authors.
Macromolecules | Year: 2011

A biomass-based and racemization-free polymer, poly(tetramethyl glycolide) (PTMG) possessing superior depolymerizability for the reproduction was developed. Renewable resources, D-/L-lactic acids and pyruvic acid derived from biomasses were employed as starting materials for the synthesis of HIBA (αhydroxyisobutyric acid). The biomass-based HIBA is prepared by methylation of the acids and then converted into the cyclic dimer. Two synthetic routes of HIBA from the renewable resources were performed. One was the direct methylation of D-/L-lactic acid derivatives after the abstraction of R-hydrogen on a chiral carbon and the other was the methylation of an R-keto group of a pyruvic acid derivative by the Grignard reaction. These results reveal PTMG as a superior recyclable material by the virtue of its controllable conversion into each monomer.


Tanaka M.,Kyushu University | Onomoto T.,Fukuoka Industrial Technology Center | Tsuchiyama T.,Kyushu University | Higashida K.,Kyushu University
ISIJ International | Year: 2012

The brittle-to-ductile transition (BDT) behaviour in nickel-free austenitic stainless steel with high nitrogen was investigated. Fall-weight impact tests revealed that Fe-25mass%Cr-1.1mass%N austenitic steel exhibits a sharp BDT behaviour in spite of an fcc alloy. The aspects of plastic deformation after the impact tests indicate that the BDT observed in this austenitic steel is induced by poor ductility at low temperatures as is the same as that in ferritic steels. In order to measure the activation energy for the BDT, the strain rate dependence of the BDT temperature was examined by using four-point bending tests. The weak dependence of the BDT temperature on the strain rate was observed. The Arrhenius plot of the BDT temperature against the strain rate elucidated that the activation energy for the BDT of Fe-25mass%Cr-1.1mass%N is much higher than that of low carbon ferritic steels. The origins of such distinct BDT behaviour and its large value of the activation energy in this high-nitrogen steel are discussed in terms of the reduction of dislocation mobility at low temperatures due to the interaction between glide dislocations and nitrogen solute atoms. © 2012 ISIJ.


Nakano K.,Fukuoka Industrial Technology Center
Chemistry Letters | Year: 2016

The influence of ultrasonication was investigated on the synthesis of nickel particles from aqueous solution including nickel hydrazine complex. Nickel nanoparticles were formed by heating up precursor solution having been prepared under ultrasonication in advance. The mean size of nickel nanoparticles decreased with increasing time of ultrasonication, and it reached 43nm with ultrasonication for 60min. © 2016 The Chemical Society of Japan.


Okumura S.,Fukuoka Industrial Technology Center | Ishikawa T.,Fukuoka Industrial Technology Center | Saitoh H.,Fukuoka Industrial Technology Center | Akao T.,Fukuoka Industrial Technology Center | Mizuki E.,Fukuoka Industrial Technology Center
Biotechnology Letters | Year: 2013

Bacillus thuringiensis A1470 produces multiple proteins with similar molecular masses (~30 kDa) with cytotoxicity against human cell lines. One that was previously identified, parasporin-4, is a β-pore-forming toxin. The N-terminal sequence of a second cytotoxic protein was identical to a partial sequence of parasporin-2 produced by B. thuringiensis A1547. PCR was performed on total plasmid DNA from A1470 by using primers for parasporin-2 to amplify a gene which was then cloned. The cloned gene differed from A1547 parasporin-2 by 8 bp and the predicted protein differed by four amino acids. The gene was expressed in Escherichia coli, and the cytotoxic activities of the recombinant protein against four human cell lines (MOLT-4, Jurkat, HeLa, and HepG2) were similar to those of A1547 parasporin-2. We then confirmed that the A1470 strain simultaneously produces parasporin-2 and parasporin-4. © 2013 Springer Science+Business Media Dordrecht.


Tsukatani T.,Fukuoka Industrial Technology Center
Nippon Shokuhin Kagaku Kogaku Kaishi | Year: 2015

To determine microbial populations and their viability, methods assessing colonies formed and turbidity are generally used. However, these methods require a long incubation time. Thus, a rapid colorimetric microbial viability assay based on the reduction of the tetrazolium salt WST-8 via 2-methyl-1,4-naphthoquinone as an electron mediator was developed. The applicability of the assay for susceptibility testing of various bacteria to antibiotics, screening of antimicrobial substances, determination of water-soluble vitamins, and differentiation of Gram-positive and - negative bacteria was investigated. Good agreement was observed between results with the WST-8 colorimetric method and those obtained using conventional methods. The present assay method was superior to the conventional methods with respect to the overall rapidity, accuracy and convenience. Copyright © 2015, Japanese Society for Food Science and Technology.


Sakai S.,Osaka University | Liu Y.,Kyushu University | Yamaguchi T.,Fukuoka Industrial Technology Center | Watanabe R.,Japan Vilene Company | And 2 more authors.
Bioresource Technology | Year: 2010

Butyl-biodiesel production using electrospun polyacrylonitrile fibers with Pseudomonas cepacia lipase immobilized through physical adsorption was studied. About 80% conversion to butyl-biodiesel was achieved after 24 h by suspending the catalyst at 2.4 mg/mL in a mixture of rapeseed oil and n-butanol at a molar ratio of 1:3, containing water at 8000 ppm at 40 °C. A further 24 h of operation resulted in 94% conversion. The initial reaction rate detected for this process was 65-fold faster than those detected for Novozym 435 on a total catalyst mass basis. The immobilized lipase continued to work as a catalyst for 27 d, within a 15% reduction in conversion yield at the outlet of the reactor compared with the average value detected during the first 3 d of operation in a continuous butyl-biodiesel production system. © 2010 Elsevier Ltd. All rights reserved.


Tsukatani T.,Fukuoka Industrial Technology Center | Suenaga H.,Fukuoka Industrial Technology Center | Shiga M.,Dojindo Laboratories | Noguchi K.,Dojindo Laboratories | And 3 more authors.
Journal of Microbiological Methods | Year: 2012

The minimum inhibitory concentrations (MICs) obtained from the susceptibility testing of various bacteria to antibiotics were determined by a colorimetric microbial viability assay based on reduction of a tetrazolium salt {2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2. H-tetrazolium, monosodium salt (WST-8)} via 2-methyl-1,4-napthoquinone as an electron mediator and compared with those obtained by the broth microdilution methods approved by the Clinical and Laboratory Standard Institute (CLSI). Especially for drug-resistant bacteria, the CLSI method at an incubation time of 24. h tended to give lower MICs. The extension of incubation time was necessary to obtain consistent MICs for drug-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococi (VRE) and multi-drug resistant Pseudomonas aeruginosa (MDRP) in the broth microdilution method. There was excellent agreement between the MICs determined after 24. h using the WST-8 colorimetric method and those obtained after 48-96. h using the broth microdilution method. The results suggest that the WST-8 colorimetric assay is a useful method for rapid determination of consistent MICs for drug-resistant bacteria. © 2012 Elsevier B.V..


Okumura S.,Fukuoka Industrial Technology Center | Saitoh H.,Fukuoka Industrial Technology Center | Ishikawa T.,Fukuoka Industrial Technology Center | Inouye K.,Kyoto University | Mizuki E.,Fukuoka Industrial Technology Center
Biochimica et Biophysica Acta - Biomembranes | Year: 2011

Parasporin-4 (PS4) is a cytotoxic protein produced by Bacillus thuringiensis strain A1470. It exhibits specific cytotoxicity against human cancer cell lines, CACO-2, Sawano, and MOLT-4 cells, in particular. When cells were administrated with PS4, cell swelling and nuclear shrinkage were induced, and, the ballooned cells burst within 24 h. PSI-BLAST search showed that the protein shared homology not only with B. thuringiensis Cry toxins but also with aerolysin-type β-pore-forming toxins. Circular dichroism measurements suggested that PS4 was a β-sheet-rich protein. PS4 aggregated into oligomers on the plasma membrane of PS4-susceptible CACO-2 cells, but not on that of PS4-resistant HeLa cells. Leakage of lactate dehydrogenase and influx of extracellular FITC-dextrans were observed only in susceptible cells. The activation of effectors caspase 3 and/or 7 was not observed in PS4-treated CACO-2 cells. It was shown that cytotoxicity of the PS4 against CACO-2 cells was exhibited when treated by cyclodextrin which induces cholesterol depletion. These results suggest that PS4 is a unique β-pore-forming toxin with a cholesterol-independent activity. © 2010 Elsevier B.V. All rights reserved.


Tsukatani T.,Fukuoka Industrial Technology Center | Suenaga H.,Fukuoka Industrial Technology Center | Shiga M.,Dojindo Laboratories | Matsumoto K.,Sojo University
Letters in Applied Microbiology | Year: 2014

A rapid microplate method for the proliferation assay of fungi and the antifungal susceptibility testing using the colorimetric microbial viability assay based on the reduction in a tetrazolium salt 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt (WST-8) with 2-methyl-1,4-napthoquinone as the electron mediator was developed. The proposed method was useful to measure the proliferation of 18 kinds of moulds and seven kinds of yeasts, including representative pathogens such as Aspergillus spp., Candida spp. and Cryptococcus spp. Linear relationships between the absorbance and viable fungal cell density were obtained for all fungi, suggesting that the absorbance change reflected the fungal proliferation. In addition, the minimum inhibitory concentrations (MICs) against a variety of different pathogenic moulds and yeasts for amphotericin B, itraconazole and 5-flucytosine were determined by susceptibility testing using the proposed method and compared with those obtained using the conventional broth microdilution method. There was an excellent agreement between the results obtained using the WST-8 colorimetric method and those obtained using the conventional Clinical and Laboratory Standard Institute method. The WST-8 colorimetric assay is a useful method for rapid determination of accurate MICs for a variety of different fungi. © 2014 The Society for Applied Microbiology.

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